Variation in neutral and polar lipid compositions of ova in ten reproductively isolated populations of walleye (Sander vitreus)

2004 ◽  
Vol 61 (1) ◽  
pp. 110-121 ◽  
Author(s):  
Murray D Wiegand ◽  
Thomas A Johnston ◽  
Jennifer Martin ◽  
William C Leggett
Keyword(s):  
Fatty Acid ◽  
Neutral Lipid ◽  
Lipid Content ◽  
Polar Lipid ◽  
Lipid Fraction ◽  
Egg Mass ◽  
Sander Vitreus ◽  
Isolated Populations ◽  
Relative Abundances ◽  
High Level

We examined variation in the mass and composition of egg lipids within and among 10 walleye (Sander vitreus) populations. Larger eggs contained more lipid, but neither absolute lipid content (mg·egg–1) nor relative lipid content (percent of egg mass as lipid) were related to maternal age or size. Among populations, the proportion of egg mass as neutral lipid varied significantly, whereas the proportion as polar lipid did not. Egg fatty acid composition did vary with respect to maternal traits. In the Lake Ontario population, larger females appear to produce a qualitatively superior egg; relative abundances of docosahexaenoic acid (22:6(n–3), DHA) and arachidonic acid (20:4(n–6), AA) increased, and the relative abundance of eicosapentaenoic acid (20:5(n–3), EPA) decreased in egg fatty acids with increasing maternal length. We predicted that fatty acid profiles would be less variable in the polar than in the neutral lipid fraction. Relative abundances of DHA, AA, the sum of AA and EPA, and several other fatty acid classes were all significantly less variable in the polar than in the neutral fraction both among females and among populations. Our results indicate a high level of conservatism of egg lipid mass and polar lipid composition in this species.

The effect of long term storage on the lipid reserves and fatty acid composition of cysts and hatched juveniles of Globodera rostochiensis and G. pallida

1998 ◽  
Vol 72 (2) ◽  
pp. 133-141 ◽  
Author(s):  
R.A. Holz ◽  
D.J. Wright ◽  
R.N. Perry
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Neutral Lipid ◽  
Lipid Content ◽  
Neutral Lipids ◽  
Lipid Fraction ◽  
Neutral Lipid Fraction ◽  
Fatty Acid Profiles ◽  
Acidic Phospholipids

AbstractThe lipid composition of three batches of single generation cysts of Globodera rostochiensis, stored dry at 4°C for 1,7 and 13 years, comprised 81%, 74% and 53% neutral lipids, 14%, 18% and 27% non-acidic phospholipids and 5%, 8% and 20% free fatty acids, respectively. Lipids in eggs from two batches of G. pallida cysts, stored for 3 and 7 years, comprised 80% and 67% neutral lipids, 15% and 23% non-acidic phospholipids and 5% and 10% free fatty acids, respectively. All batches contained the same fatty acids which were dominated by C18:l, C20:l and C20:4. The fatty acid profiles of hatched J2 of G. rostochiensis from two batches, stored for 1 and 9 years, differed only in their free fatty acid fractions. Thus, while it is not possible to determine the age of cysts by their fatty acid profile, it may be possible to use the relative amounts of the main lipid classes as an indicator of age. Four batches of hatched J2 of G. pallida were investigated, with sample A hatched during the second week in potato root diffusate, B during week 3, C during week 4 and D during weeks 5 and 6 and stored for 3.5 days (on average) after hatching. Total lipid content was 27.2%, 31.5%, 18.5% and 6.3% of the dry weight for A, B, C and D, respectively. In the neutral lipid fraction of D an increase in C18:l and to a lesser extent C18:2 was observed. In the free fatty acid fraction of sample D, the percentages of C18:l, C18:2 and C18:3 were greater but the percentages of C20:3 and C20:4 were smaller compared with sample C. Fresh early hatched J2 of G. rostochiensis were compared with later hatched and stored (for 13 days on average) individuals for their lipid content and fatty acid composition. The lipid content was 26.1% and 11.4% in fresh and stored J2, respectively. Total lipid consisted of 77% and 70% neutral lipid, 18% and 26% non-acidic phospholipid and 6% and 4% free fatty acid in fresh and stored J2, respectively. In the neutral lipid fraction of stored J2 C18:l, C16:0 and C18:0 increased, whereas C20:4, C20:l and C20:3 decreased. Therefore, both neutral lipid and free fatty acid fractions showed changes in their fatty acid profiles after long delayed hatching and/or storage in both PCN species.

Neutral lipids, phospholipids, and a betaine lipid of the snow mold fungus Microdochium nivale

1998 ◽  
Vol 44 (11) ◽  
pp. 1051-1059 ◽  
Author(s):  
Anita Istokovics ◽  
Naoki Morita ◽  
Kazuo Izumi ◽  
Tamotsu Hoshino ◽  
Isao Yumoto ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Neutral Lipid ◽  
Polar Lipid ◽  
Lipid Fraction ◽  
Microdochium Nivale ◽  
Neutral Lipid Fraction ◽  
Polar Lipid Fraction ◽  
Snow Mold ◽  
Betaine Lipid

The hyphae of the snow mold Microdochium nivale contained lipids in a yield of about 10% w/w of the dry matter of hyphae. The total lipid was fractionated into neutral and polar lipid fractions. In the neutral lipid fraction, triacylglylcerol was the sole major component. As minor components, ergosterol, diacylglycerol, free fatty acid, and fatty acyl ergosterol were identified. The polar lipid fraction contained phospholipids, glycolipids, and a lipid containing neither phosphorus nor sugar. Phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, and phosphatidic acid were identified as phospholipids. The polar lipid fraction included at least four kinds of glycolipids that have not been identified. A very unusual lipid in fungi, a betaine lipid, diacylglyceryltrimethylhomoserine, was identified by chemical and physicochemical analyses. The level of the neutral lipid fraction, which accounted for 60% of the total lipid in hyphae at the exponential phase, was significantly increased compared with that of the polar lipid fraction and constituted 80% of the total at the stationary phase. The neutral and polar lipids of Microdochium nivale contained 18:3 (9,12,15), 18:2 (9,12), 18:1 (9), and 16:0 as principal fatty acids. Among them, 18:2 (9,12) and 18:3 (9,12,15) were the major fatty acids of triacylglycerol, phosphatidylcholine, phosphatidylglycerol, and phosphatidylethanolamine, whereas in diacylglyceryltrimethylhomoserine, the major components were 16:0 and 18:3 (9,12,15).Key words: snow mold, phospholipids, betaine lipid, fatty acid, Microdochium nivale.

scholarly journals Seasonal Variations of Lipid Content and Composition in Starfish Asterias amurensis Lütken

2012 ◽  
Vol 15 (1) ◽  
pp. 45 ◽  
Author(s):  
A.K.M. Azad Shah ◽  
H. Kurihara ◽  
K. Takahashi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Content ◽  
Polar Lipid ◽  
Lipid Fraction ◽  
Total Lipid Content ◽  
Polar Lipid Fraction ◽  
Internal Organs ◽  
Asterias Amurensis ◽  
Lipid Fractions

The total lipid content, lipid classes and fatty acid composition in the internal organs of starfish (Asterias amurensis) were analyzed to determine the effects of seasons (winter and spring). The non-polar and polar lipid fractions obtained from starfish internal organs were analyzed through two seasons using thin layer chromatography (TLC) and gas liquid chromatography. Total lipid content of internal organs was 10.18% in spring and 8.21% in winter as wet weight basis. The predominant lipid class in spring was triglyceride whereas free fatty acids were the main lipid class in winter. The most abundant fatty acid of non-polar lipid fraction was eicosamonoenoic acid (C20:1) in spring having the highest proportion (29.2% of total fatty acid) as compared to the winter. Eicosapentaenoic acid (EPA) was also found significantly (P < 0.05) higher in spring compared to winter in the non-polar lipid fraction. On the other hand, comparatively lower amount of EPA was observed in spring than winter in the polar lipid fraction. Proportions of other fatty acids in non-polar and polar lipid fractions were also varied seasonally. This result might be useful for commercial production of lipid from internal organ of starfish with a view to potential use in food, pharmaceutical, cosmetics and other non-food industries.

The fatty acid composition of conidia of Aspergillus niger V. Tiegh.

1972 ◽  
Vol 18 (10) ◽  
pp. 1575-1576 ◽  
Author(s):  
M. Gunasekaran ◽  
W. M. Hess ◽  
D. J. Weber
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Aspergillus Niger ◽  
Lipid Content ◽  
Polar Lipid ◽  
Lipid Fraction ◽  
Lipid Bodies ◽  
Neutral Lipid Fraction ◽  
Total Fatty Acids

The lipids from conidia of Aspergillus niger were extracted and identified. The polar lipid content was higher (54.3%) than the neutral lipid fraction (45.7%). The major fatty acids present were C16:0, C18:0, C18:1, and C18:2. The amount of unsaturation in total fatty acids (62%) was higher than the unsaturation in the polar lipids (35%). Freeze-etched replicas of dormant conidia revealed the presence of lipid bodies.

scholarly journals Hypolipidemic effects of herbal extracts by reduction of adipocyte differentiation, intracellular neutral lipid content, lipolysis, fatty acid exchange and lipid droplet motility

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Renate Haselgrübler ◽  
Peter Lanzerstorfer ◽  
Clemens Röhrl ◽  
Flora Stübl ◽  
Jonas Schurr ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Neutral Lipid ◽  
Lipid Droplet ◽  
Lipid Content ◽  
Adipocyte Differentiation ◽  
Herbal Extracts ◽  
Neutral Lipid Content ◽  
Acid Exchange

NEUTRAL LIPID CONTENT AND FATTY ACID COMPOSITION OF 1,2-DIACYLGLYCEROLS IN THE HEMOLYMPH OF THE MALE GYPSY MOTH, LYMANTRIA DISPAR L. (LEPIDOPTERA: LYMANTRIIDAE)

1990 ◽  
Vol 122 (6) ◽  
pp. 1101-1109 ◽  
Author(s):  
J. Marshall Clark ◽  
J.R. Marion ◽  
L.J. Scarano ◽  
T.L. Potter ◽  
P.F. Gosselin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Neutral Lipid ◽  
Acid Composition ◽  
Adult Male ◽  
Lipid Content ◽  
Lymantria Dispar ◽  
Mass Spectrometry Analysis ◽  
Neutral Lipid Content

AbstractQualitative and quantitative changes in the neutral lipid content of hemolymph of gypsy moths, Lymantria dispar L., were assayed in larval, pupal, and adult stages. The major neutral lipid constituents of the hemolymph were 1,2-diacylglycerols and ranged in nominal concentration from 1.6–3.4 (larval), 3.1–4.9(pupal),toamaximumof 19.3 μg lipid per microlitre hemolymph in the adult male. When detected, triacylglycerols and monoacylglycerols never exceed diacylglycerol concentrations. The fatty acid composition of 1,2-diacylglycerols from adult male moths (0–12 h after emergence) was determined as fatty acid methyl esters using gas chromatography/mass spectrometry analysis. Nine fatty acid structures have been assigned. Of these, five are saturated, unbranched, aliphatic fatty acids (C14:0 – C18:0) which comprise 80.5% of the total fatty acid abundance. The remaining four fatty acids consist of two saturated, methyl-branched, aliphatic compounds, a mono-unsaturated aliphatic acid, and a tri-unsaturated, tricyclic, diterpenoid acid.

Contrasting strategies of ova lipid provisioning in relation to maternal characteristics in three walleye (Sander vitreus) populations

2007 ◽  
Vol 64 (4) ◽  
pp. 700-712 ◽  
Author(s):  
M D Wiegand ◽  
T A Johnston ◽  
W C Leggett ◽  
K E Watchorn ◽  
A J Ballevona ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Content ◽  
Maternal Age ◽  
Lake Ontario ◽  
The Other ◽  
Sander Vitreus ◽  
Selection Pressures ◽  
Lake Winnipeg ◽  
Maternal Characteristics ◽  
Nutrient Reserves

We examined how the lipid content and fatty acid composition of walleye (Sander vitreus) ova varied with respect to maternal characteristics, particularly indices of body nutrient reserves, within three spawning populations that varied in maternal age, size, and somatic lipid reserves. We also compared the variability in ova lipid composition among three populations with that observed among spawning years within one of these populations. Maternal characteristics had little influence on ova lipid content. In Lake Ontario, percentages of docosahexaenoic acid (22:6(n-3), DHA) and arachidonic acid (20:4(n-6), AA) increased with maternal length, while those of eicosapentaenoic acid (20:5(n-3), EPA) declined. In Lake Nipissing, maternal age had similar effects on AA and EPA, but not DHA. The Lake Winnipeg population did not conform to the trends of the other two populations, and ova from this population had very low levels of AA. We hypothesize that there are similar selection pressures on the Lake Ontario and Lake Nipissing populations with respect to desirable ova fatty acid profiles. Lake Winnipeg walleye may experience different selection pressures or may be unable to conform to the trends observed in the other two lakes.

The metabolism of exogenous fatty acids by preimplantation mouse embryos developing in vitro

Development ◽  
1980 ◽  
Vol 56 (1) ◽  
pp. 157-168
Author(s):  
Thomas J. Flynn ◽  
Nina Hillman
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Palmitic Acid ◽  
Neutral Lipid ◽  
Polar Lipid ◽  
Mouse Embryos ◽  
Exogenous Fatty Acid ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse

The utilization of fatty acids from the culture medium has been examined in preimplantation mouse embryos developing in vitro. Incorporation of exogenous fatty acid into embryo lipids was examined by culturing 8-cell mouse embryos for 2 h in a medium containing 0·1 HIM [9, 10-3H]palmitic acid (900 mCi/mmol). Lipids were extracted from the embryos, and the total lipid extract was fractionated into various neutral lipid and polar lipid classes by thin-layer chromatography. Most of the radioactivity, over 93 %, was recovered in neutral glycerides (mono- di-, and triacylglycerols). about 2% of the total radioactivity was recovered in other neutral lipid species including fatty acids, fatty alcohols, and sterol esters. The remainder of the radioactivity was recovered in polar lipids. Seventy-four per cent of the polar lipid radioactivity was present in the choline phosphatides. Other labelled phospholipid and glycolipid species included ethanolamine phosphatides, inositol and/or serine phosphatides, sphingomyelin, choline lysophosphatides, sulfatides, cerebrosides, and monoglycosylglycerides. Chemical degradation studies of labelled embryo lipids indicated that the tritium label was entering into embryo lipids as the fatty acid and not via metabolic recycling. The oxidation of exogenous fatty acids by mouse embryos was assessed by incubating variously staged embryos for 4h in medium containing 0·1 mM [U-14C]palmitic acid (50 mCi/mmol) and quantitating the production of 14CO2. The rate of fatty acid oxidation was found to be relatively constant from the unfertilized egg up to the 8-cell stage and then increase significantly between the 8-cell and late blastocyst stages. The results suggest that preimplantation mouse embryos developing in vitro can utilize fatty acids from the medium both for incorporation into embryo lipids and for energy production via oxidation.

Changes in esterified fatty acids in the isolated, perfused rabbit heart

1968 ◽  
Vol 46 (10) ◽  
pp. 1241-1246 ◽  
Author(s):  
K. Kako ◽  
M. J. G. Dubuc
Keyword(s):  
Fatty Acid ◽  
Left Ventricle ◽  
Neutral Lipid ◽  
Lipid Fraction ◽  
Fatty Acid Distribution ◽  
Rabbit Heart ◽  
Left Ventricular ◽  
Buffer Solution ◽  
The Right ◽  
Lipid Stores

The hearts of fasted (48 h) or fed rabbits were perfused with buffer solution with or without glucose. The right ventricle was removed at the 10th min of perfusion. The left ventricle was perfused for 10, 70, or 130 min. Cardiac lipids were analyzed by the hydroxamic acid method and gas chromatography. The neutral lipid fraction from the heart of the fasted rabbit contained relatively more linoleic and less palmitic acid than that from the heart of the fed animal. Similarly, relatively more oleic and less stearic acids were found in the phospholipid fraction from the heart of the fasted rabbit. There was a slight difference in fatty acid distribution between the right and left ventricular lipids at the 10th min of perfusion.The left ventricle of the fasted rabbit lost two-thirds of its neutral lipid stores during perfusion for 120 min with a substrate-free medium. Some of the phospholipid also disappeared between the 70th and the 130th min of perfusion. No measurable loss of lipids was found in the hearts of fed animals perfused under the same conditions. In the presence of glucose in the perfusing medium, cardiac lipid contents remained unchanged during perfusion regardless of the nutritional state of the animal. Esterified fatty acid composition did not change significantly during perfusion. The significance of these observations is discussed in relation to possible metabolic control systems in fasted and fed animals.

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