Contrasting strategies of ova lipid provisioning in relation to maternal characteristics in three walleye (Sander vitreus) populations

2007 ◽  
Vol 64 (4) ◽  
pp. 700-712 ◽  
Author(s):  
M D Wiegand ◽  
T A Johnston ◽  
W C Leggett ◽  
K E Watchorn ◽  
A J Ballevona ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Content ◽  
Maternal Age ◽  
Lake Ontario ◽  
The Other ◽  
Sander Vitreus ◽  
Selection Pressures ◽  
Lake Winnipeg ◽  
Maternal Characteristics ◽  
Nutrient Reserves

We examined how the lipid content and fatty acid composition of walleye (Sander vitreus) ova varied with respect to maternal characteristics, particularly indices of body nutrient reserves, within three spawning populations that varied in maternal age, size, and somatic lipid reserves. We also compared the variability in ova lipid composition among three populations with that observed among spawning years within one of these populations. Maternal characteristics had little influence on ova lipid content. In Lake Ontario, percentages of docosahexaenoic acid (22:6(n-3), DHA) and arachidonic acid (20:4(n-6), AA) increased with maternal length, while those of eicosapentaenoic acid (20:5(n-3), EPA) declined. In Lake Nipissing, maternal age had similar effects on AA and EPA, but not DHA. The Lake Winnipeg population did not conform to the trends of the other two populations, and ova from this population had very low levels of AA. We hypothesize that there are similar selection pressures on the Lake Ontario and Lake Nipissing populations with respect to desirable ova fatty acid profiles. Lake Winnipeg walleye may experience different selection pressures or may be unable to conform to the trends observed in the other two lakes.

Variation in neutral and polar lipid compositions of ova in ten reproductively isolated populations of walleye (Sander vitreus)

2004 ◽  
Vol 61 (1) ◽  
pp. 110-121 ◽  
Author(s):  
Murray D Wiegand ◽  
Thomas A Johnston ◽  
Jennifer Martin ◽  
William C Leggett
Keyword(s):  
Fatty Acid ◽  
Neutral Lipid ◽  
Lipid Content ◽  
Polar Lipid ◽  
Lipid Fraction ◽  
Egg Mass ◽  
Sander Vitreus ◽  
Isolated Populations ◽  
Relative Abundances ◽  
High Level

We examined variation in the mass and composition of egg lipids within and among 10 walleye (Sander vitreus) populations. Larger eggs contained more lipid, but neither absolute lipid content (mg·egg–1) nor relative lipid content (percent of egg mass as lipid) were related to maternal age or size. Among populations, the proportion of egg mass as neutral lipid varied significantly, whereas the proportion as polar lipid did not. Egg fatty acid composition did vary with respect to maternal traits. In the Lake Ontario population, larger females appear to produce a qualitatively superior egg; relative abundances of docosahexaenoic acid (22:6(n–3), DHA) and arachidonic acid (20:4(n–6), AA) increased, and the relative abundance of eicosapentaenoic acid (20:5(n–3), EPA) decreased in egg fatty acids with increasing maternal length. We predicted that fatty acid profiles would be less variable in the polar than in the neutral lipid fraction. Relative abundances of DHA, AA, the sum of AA and EPA, and several other fatty acid classes were all significantly less variable in the polar than in the neutral fraction both among females and among populations. Our results indicate a high level of conservatism of egg lipid mass and polar lipid composition in this species.

Metabolic changes in biotin-deficient Aspergillus nidulans

1968 ◽  
Vol 14 (7) ◽  
pp. 813-815 ◽  
Author(s):  
K. K. Rao ◽  
V. V. Modi
Keyword(s):  
Fatty Acid ◽  
Protein Content ◽  
Aspergillus Nidulans ◽  
Lipid Content ◽  
Considerable Increase ◽  
Metabolic Changes ◽  
The Other ◽  
Biotin Deficiency ◽  
Mitochondrial Integrity ◽  
General Metabolism

Biotin deficiency has been found to result in greatly diminished ability to synthesize fatty acid, causing decrease in the lipid content of the mold. This decrease is accompanied by considerable increase in protein content of the mat.There is 30% reduction in lipid content of the mitochondria of the biotin-deficient molds. This should have resulted in the loss of mitochondrial integrity affecting the general metabolism of A. nidulans. On the other hand, under these conditions the mat weight of the mold increases.

Preliminary Study of Lipid Content and Fatty Acid Composition in the Spottail Pinfish, Diplodus holbrooki

Copeia ◽  
1999 ◽  
Vol 1999 (3) ◽  
pp. 766 ◽  
Author(s):  
Ileana E. Clavijo ◽  
Carolyn S. Dunn ◽  
Pamela J. Seaton
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Lipid Content ◽  
Preliminary Study

scholarly journals Identification of the molecular regulation of differences in lipid deposition in dedifferentiated preadipocytes from different chicken tissues

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Zheng Ma ◽  
Na Luo ◽  
Lu Liu ◽  
Huanxian Cui ◽  
Jing Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Lipid Content ◽  
Regulatory Network ◽  
Total Lipid Content ◽  
Fatty Acid Transport ◽  
Lipid Deposition ◽  
Ppar Signaling ◽  
The Difference

Abstract Background A body distribution with high intramuscular fat and low abdominal fat is the ideal goal for broiler breeding. Preadipocytes with different origins have differences in terms of metabolism and gene expression. The transcriptome analysis performed in this study of intramuscular preadipocytes (DIMFPs) and adipose tissue-derived preadipocytes (DAFPs) aimed to explore the characteristics of lipid deposition in different chicken preadipocytes by dedifferentiation in vitro. Results Compared with DAFPs, the total lipid content in DIMFPs was reduced (P < 0.05). Moreover, 72 DEGs related to lipid metabolism were screened, which were involved in adipocyte differentiation, fatty acid transport and fatty acid synthesis, lipid stabilization, and lipolysis. Among the 72 DEGs, 19 DEGs were enriched in the PPAR signaling pathway, indicating its main contribution to the regulation of the difference in lipid deposition between DAFPs and DIMFPs. Among these 19 genes, the representative APOA1, ADIPOQ, FABP3, FABP4, FABP7, HMGCS2, LPL and RXRG genes were downregulated, but the ACSL1, FABP5, PCK2, PDPK1, PPARG, SCD, SCD5, and SLC27A6 genes were upregulated (P < 0.05 or P < 0.01) in the DIMFPs. In addition, the well-known pathways affecting lipid metabolism (MAPK, TGF-beta and calcium) and the pathways related to cell communication were enriched, which may also contribute to the regulation of lipid deposition. Finally, the regulatory network for the difference in lipid deposition between chicken DAFPs and DIMFPs was proposed based on the above information. Conclusions Our data suggested a difference in lipid deposition between DIMFPs and DAFPs of chickens in vitro and proposed a molecular regulatory network for the difference in lipid deposition between chicken DAFPs and DIMFPs. The lipid content was significantly increased in DAFPs by the direct mediation of PPAR signaling pathways. These findings provide new insights into the regulation of tissue-specific fat deposition and the optimization of body fat distribution in broilers.

An unconventional two-stage cultivation strategy to increase the lipid content and enhance the fatty acid profile on Chlorella minutissima biomass cultivated in a novel internal light integrated photobioreactor aiming at biodiesel production

2020 ◽  
Vol 156 ◽  
pp. 591-601
Author(s):  
Mateus S. Amaral ◽  
Carla C.A. Loures ◽  
Guilherme A. Pedro ◽  
Cristiano E.R. Reis ◽  
Heizir F. De Castro ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Lipid Content ◽  
Biodiesel Production ◽  
Two Stage ◽  
Cultivation Strategy

scholarly journals miR-21-5p regulates mitochondrial respiration and lipid content in H9C2 cells

2019 ◽  
Vol 316 (3) ◽  
pp. H710-H721 ◽  
Author(s):  
Victoria L. Nasci ◽  
Sandra Chuppa ◽  
Lindsey Griswold ◽  
Kathryn A. Goodreau ◽  
Ranjan K. Dash ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acid ◽  
Palmitic Acid ◽  
Fatty Acid Oxidation ◽  
Lipid Content ◽  
Mitochondrial Respiration ◽  
Acid Oxidation ◽  
H9c2 Cells ◽  
Cellular Lipid ◽  
Transfected Cells

Cardiovascular-related pathologies are the single leading cause of death in patients with chronic kidney disease (CKD). Previously, we found that a 5/6th nephrectomy model of CKD leads to an upregulation of miR-21-5p in the left ventricle, targeting peroxisome proliferator-activated receptor-α and altering the expression of numerous transcripts involved with fatty acid oxidation and glycolysis. In the present study, we evaluated the potential for knockdown or overexpression of miR-21-5p to regulate lipid content, lipid peroxidation, and mitochondrial respiration in H9C2 cells. Cells were transfected with anti-miR-21-5p (40 nM), pre-miR-21-5p (20 nM), or the appropriate scrambled oligonucleotide controls before lipid treatment in culture or as part of the Agilent Seahorse XF fatty acid oxidation assay. Overexpression of miR-21-5p attenuated the lipid-induced increase in cellular lipid content, whereas suppression of miR-21-5p augmented it. The abundance of malondialdehyde, a product of lipid peroxidation, was significantly increased with lipid treatment in control cells but attenuated in pre-miR-21-5p-transfected cells. This suggests that miR-21-5p reduces oxidative stress. The cellular oxygen consumption rate (OCR) was increased in both pre-miR-21-5p- and anti-miR-21-5p-transfected cells. Levels of intracellular ATP were significantly higher in anti-mR-21-5p-transfected cells. Pre-miR-21-5p blocked additional increases in OCR in response to etomoxir and palmitic acid. Conversely, anti-miR-21-5p-transfected cells exhibited reduced OCR with both etomoxir and palmitic acid, and the glycolytic capacity was concomitantly reduced. Together, these results indicate that overexpression of miR-21-5p attenuates both lipid content and lipid peroxidation in H9C2 cells. This likely occurs by reducing cellular lipid uptake and utilization, shifting cellular metabolism toward reliance on the glycolytic pathway. NEW & NOTEWORTHY Both overexpression and suppression of miR-21-5p augment basal and maximal mitochondrial respiration. Our data suggest that reliance on glycolytic and fatty acid oxidation pathways can be modulated by the abundance of miR-21-5p within the cell. miR-21-5p regulation of mitochondrial respiration can be modulated by extracellular lipids.

scholarly journals Effect of Dietary Fat Composition and 2’Fucosyllactose on Neonatal Piglet Growth and Organ Lipid Composition

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1038-1038
Author(s):  
Michael Miklus ◽  
Pedro Prieto ◽  
Cynthia Barber ◽  
Robert Rhoads ◽  
Samer El-Kadi
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Fatty Acid ◽  
Body Weight Gain ◽  
Dietary Fatty Acids ◽  
The Body ◽  
The Other ◽  
Human Infant ◽  
Lps Challenge ◽  
Group 3

Abstract Objectives The objectives of this study were to determine the effect of 2’fucosyllactose (2’FL) and fat blends on growth, body composition and fatty acid profile of the liver and brain using the neonatal pig as a model for the human infant. Methods Pigs (3 d old) were randomly assigned to either: 1. control, 2. Palm Olein (PO) fat blend – Low 2'-FL, 3. PO – High 2'-FL, 4. High oleic acid (HO) – Low 2'-FL, 5. HO FB – High 2'-FL, 6. PO FB – GLA, or 7. kept with their sows. Pigs in groups 1 to 6 received 250 ml·kg−1·d−1 of formula in 5 equal meals for 15 d. On day 14 of the study, groups 1–6 received intraperitoneal E. coli LPS challenge at 100 µg·kg−1 weight. Results Body weight was greater for piglets fed by sows than those in the other groups (P &lt; 0.001). In addition, % fat and bone mineral content were higher in the sow-fed group while lean % was less sow-fed piglets (group 7) compared with those in the other groups (P &lt; 0.05). Only longissimus weight expressed as a % of body weight, was greater for group 7 compared with all other groups (P &lt; 0.001). Soleus, semitendinosus, brain, heart and spleen weights as a % of body weight were similar across all groups. However, liver weight as a % of body weight was greater in groups 1–6 (3.7%) compared with group 7 (2.8%; P &lt; 0.001). The proportion of brain 16:1 fatty acid was less (0.83%) for groups 1–6 than for group 7 pigs (1.08%; P &lt; 0.0001). The proportion of 20:3 N6 was greatest (0.66%) for group 3 compared with groups 1 and 4 (0.55%; P &lt; 0.05). In addition, the proportion of 20:5 N3 was greatest (0.12%) for group 3 compared with groups 1 and 7 (0.07%; P &lt; 0.05). The proportion of liver 16:1, 18:0, and 18:1 cis-11 fatty acids were greater for group 7 (2.3, 23, 2.2%) than groups 1–6 (0.2, 20, 1.2%; P &lt; 0.0001). Conversely, the contribution of 14:0, 18:1 cis-9, 18:3 N6 cis-6,9,12, and 22:6 N3 were greater for pigs in groups 1–6 (1.3, 0.6, and 14, 7.8%) compared with those in group 7 (0.5, 8.5, 0.2 and 3.5%; P &lt; 0.0001). Conclusions Our data suggest that feeding 2’fucosyllactose had no effect on the body weight gain and composition in neonatal pigs. Our data also suggest that dietary fatty acids have a greater effect on liver than on brain fatty acid composition. Funding Sources Funding for the work was provided by Perrigo Nutritionals, LLC.

Cholesterol, Lipid Content, and Fatty Acid Composition of Different Tissues of Farmed Cobia (Rachycentron canadum) from China

2009 ◽  
Vol 86 (12) ◽  
pp. 1155-1161 ◽  
Author(s):  
Shu Cheng Liu ◽  
De Tao Li ◽  
Peng Zhi Hong ◽  
Chao Hua Zhang ◽  
Hong Wu Ji ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Lipid Content ◽  
Rachycentron Canadum ◽  
Different Tissues
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