EFFECTS OF LOW TEMPERATURES ON THE GROWTH OF MARINE BACTERIA

1933 ◽  
Vol 8 (1) ◽  
pp. 489-505 ◽  
Author(s):  
ERNEST HESS

Growth of Pseudomonas fluorescens, Flavobacterium deciduosum, and B. vulgatus (all of marine origin) in buffered nutrient broth at 37°, 20°, 5°, 0° and −3 °C. has been followed quantitatively by means of plate counts. Maximum crops were obtained at 5 °C. in all cases, and higher total crops at 0° and −3° than at 37° and 20°. Maximum crop yield is considered a better criterion for optimum growth temperature than the growth rate during logarithmic growth. These marine bacteria are therefore considered to be truly psychrophilic.


2002 ◽  
Vol 65 (7) ◽  
pp. 1179-1182 ◽  
Author(s):  
JOSEFINA BELLOQUE ◽  
ALFONSO V. CARRASCOSA

The degradation of natural phosphorylated compounds (galactose-1-phosphate, N-acetyl-glucosamine-1-phosphate, glycerophosphoethanolamine, and glycerophosphocholine) and added phosphorylated compounds (diphosphate) in milk was investigated by phosphorus 31 nuclear magnetic resonance on the incubation of a sterile milk with Pseudomonas fluorescens CECT381, Lactococcus lactis CECT539, and Kluyveromyces marxianus CECT10584. This preliminary study showed that the degradation of these compounds was dependent on the compound, microorganism, and temperature of incubation. K. marxianus CECT10584 did not show any capability to degrade these compounds, and L. lactis CECT539 was only able to degrade diphosphate at its optimum growth temperature. P. fluorescens CECT381 was the most active strain and possessed more hydrolytic capabilities at 10°C than at its optimum growth temperature. It is suggested that cold-induced enzymes are involved in the ability of P. fluorescens CECT381 to hydrolyze the natural phosphorylated compounds in milk. Consequent potential alterations of dairy products are discussed.



1975 ◽  
Vol 21 (10) ◽  
pp. 1553-1559 ◽  
Author(s):  
William H. Lynch ◽  
Janet MacLeod ◽  
Mervyn Franklin

The effect of the growth temperature, on the accumulation of glucose-oxidation products, was examined in aerated cultures with carbon excess in two strains of Pseudomonas fluorescens. At low growth temperatures (0 and 5 °C), 2-ketogluconate (KG) accumulated in the medium as the major oxidation product of glucose (up to 70%) before further metabolism. As the growth temperature was increased, the amount of 2-KG found to accumulate in the medium from glucose oxidation decreased. At a growth temperature of 20 °C, up to 25% of the glucose originally added accumulated in the medium as 2-KG. At the optimum growth temperature of 30 °C or above, no 2-KG was detected at any time during growth with glucose. Similar results were obtained when gluconate was used as the sole carbon and energy source.The results demonstrated a differential effect of growth temperature on the accumulation of oxidation products from glucose and gluconate. At low growth temperatures the major route for the catabolism of glucose and gluconate was the direct oxidative non-phosphorylated pathway.



1982 ◽  
Vol 28 (3) ◽  
pp. 284-290 ◽  
Author(s):  
N. Canillac ◽  
M. T. Pommier ◽  
A. M. Gounot

Lipid composition of three Arthrobacter strains (mesophilic, psychrotrophic, and psychrophilic strains) grown at their optimum growth temperature was studied. Great differences appeared only in the nature of their fatty acids: the psychrophilic strain synthesized less linear acids, C17 acids, and more iso isomers than the other two strains.Incubation of the three strains at temperatures below their optimum resulted in variations only in proportion of the different fatty acids: increase of the ratio of unsaturated, of branched, and of short-chain fatty acids.The relation between lipid composition and ability to grow at temperatures around 0 °C is discussed.



1995 ◽  
Vol 405 ◽  
Author(s):  
Sung-Bock Kim ◽  
Jeong-Rae Ro ◽  
Seong-Ju Park ◽  
El-Hang Lee

AbstractWe have found that the lateral dimension of InGaAs and GaAs multiple layers can be effectively controlled on non-( 111) V-grooved GaAs substrates by chemical beam epitaxy using triethylgallium and trimethylindium coupled with precracked arsine or unprecracked monoethylarsine. We suggest that this effect is due to the efficient migration of adatoms from (111) to non-(111) planes. This is an improved method which overcomes the difficulty that has been associated with the method of using only (111) V-grooves in which the lateral dimension is controlled by the differences in the growth rates between (111) and (100) planes. In case of InGaAs and GaAs epilayers, the anisotropy factors of growth rate were less than 0.1 at optimum growth temperature. Photoluminescence peak originated from InGaAs/GaAs quantum wire was significantly distinct from other peaks, suggesting an effective reduction of InGaAs lateral dimension.



1995 ◽  
Vol 45 (1-2) ◽  
pp. 93-98 ◽  
Author(s):  
Zhaodong Nan ◽  
Yongjun Liu ◽  
Haitao Sun ◽  
Honglin Zhang ◽  
Shan Qingzhu ◽  
...  


2011 ◽  
Vol 356-360 ◽  
pp. 3-7
Author(s):  
Li Hua Lv ◽  
Jing Cheng Ren ◽  
Tian Hu Sun ◽  
Jian Qing Dai

we take mining water from Liang Zou group undergroud 200 m. and cltured in 9K medium, sodium thiosulfate medium, sulfur (S0) medium. According to its conformation on the different mediums, we get ferrooxidans(T.f), and studying its ecological nature shows that the ferrooxidanis is acidophilic autotrophic bacteria and has ferrous iron and sulfur oxidation. Its optimum growth pH value is 2.0, optimum growth temperature is 30°C, 1.0 g/L of Fe2 + and 1 % S0 which can be used as the energy source are propitious to its growth. After UV mutagenesis, the strain which has been cold treated improve Fe2+ oxidation ability, at the same time, shorten the time enter to the logarithmic growth phase, the density of bacteria in lag growth phase increases from 6.8×107 cells/mL to 7.8×107 cells/mL, so increase bio-leaching capability.



2014 ◽  
Vol 989-994 ◽  
pp. 747-750 ◽  
Author(s):  
Chun Ying Yuan ◽  
Shuo Yang ◽  
Yue Wang ◽  
Qing Man Cui

Under the laboratory conditions, the effect of temperature (10, 15, 20, 25, 30°C) on growth and biochemical composition of Sargassum muticum was studied, the results showed that: the optimum growth temperature of S.muticum was 15 °C in the range of 10-30 °C; the contents of chlorophyll a, carotenoid, soluble protein, soluble sugar and brown algae polyphenols were the highest at the temperature of 25 °C, it was speculated that these components appeared compensatory increase duo to the high temperature stress. The contents of these biochemical components were the lowest at 30 °C.



1960 ◽  
Vol 27 (2) ◽  
pp. 221-234 ◽  
Author(s):  
Constance Higginbottom ◽  
Margaret M. Taylor

SummaryThe sterilization of homogenized milk at 115·5°C for 15 min in bottles having a partial vacuum in the headspace produced conditions inhibitory to the growth from very small numbers of spores ofBacillus subtilis, B. licheniformis, B. cereusandB. breviswhen compared with growth in the same milk sterilized in open bottles.B. circulansdiffered from the other strains tested in showing greater inhibition in milk sterilized in open bottles than in milk sterilized under partial vacuum.The extent of the inhibition became less as the size of the inoculum was increased. It became less also as the temperature of incubation approached the optimum growth temperature of the bacillus, and was influenced by the strain of the bacillus and the source of the milk but not by the degree of heat treatment within the range 107–117·5°C for 15 min. Inhibition was manifested by a prolongation of the lag phase, and in addition with some strains inhibition of spore germination could be demonstrated.Spore formation following vegetative growth occurred more readily in milk sterilized in open than in evacuated bottles.Milks sterilized under partial vacuum frequently failed to show any growth from small inocula in 30 days at 22°C although growth occurred readily in milk sterilized in open bottles.



2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2865-2869 ◽  
Author(s):  
Takao Iino ◽  
Mitsuo Sakamoto ◽  
Moriya Ohkuma

The facultatively aerobic, non-hydrogenotrophic, iron (Fe0)-corroding, nitrate-reducing Prolixibacter sp. strain MIC1-1T was characterized for representation of a novel species of the genus Prolixibacter. Strain MIC1-1T grew optimally at 35–37 °C, at pH 6.5 and with 2  % (w/v) NaCl. Strain MIC1-1T also grew fermentatively on some pentoses, hexoses, disaccharides and soluble starch. Succinic acid was the major end-product from d-glucose fermentation. Strain MIC1-1T was differentiated from the type strain of Prolixibacter bellariivorans by cell size, optimum growth temperature, range of temperature and NaCl for growth, and nitrate reduction. On the basis of phenotypic features and the phylogenetic position, a novel species of the genus Prolixibacter is proposed for strain MIC1-1T, to be named Prolixibacter denitrificans sp. nov. The type strain is MIC1-1T ( = JCM 18694T = NBRC 102688T = DSM 27267T). Emended descriptions of the genus Prolixibacter and Prolixibacter bellariivorans are also provided.



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