Improvements in Quantifying the Phosphorus Concentration in Lake Water
Vertical and horizontal patterns in limnetic phosphorus concentrations ([P]) were detected in an oligotrophic lake by dividing the total phosphorus pool into two fractions: dissolved and particulate matter smaller than 250 μm (smaller fraction) and particulate matter larger than 250 μm (larger fraction). The smaller fraction was estimated from samples collected with a water bottle, and the larger fraction was estimated with tow net samples taken at several stations and to various depths. Our samples were digested with potassium persulfate which gave less variable results than other acid digestion techniques. The average variance associated with the mean [P] (n = 3) for samples collected and analyzed according to our procedure was less than 0.05 mg P/m3. During summer stratification there was a consistent metalimnetic maximum in the smaller fraction, and there were small but significant differences in the concentrations found at two stations less than 1 km apart. During the same period the larger fraction was a significant portion (14–28%) of the phosphorus pool in the epilimnion which varied from 3 to 5 m in depth. It was a relatively constant portion of the phosphorus in the trophogenic zone (0–10 m) and in the 0- to 20-m portion of the water column i.e. 10–14% and 7.3–8.8%, respectively. In a separate experiment it was shown that by removing the larger fraction, the average variance associated with the mean [P] was reduced from 1.0, to 2.4 × 10−2 mg/m3. This reduction occurred because the larger fraction contained zooplankters with relatively high but variable amounts of phosphorus, and which occur in densities too low to be adequately sampled with the smaller fraction.Key words: phorphorus, lakes, phosphorus in zooplankton