Normal Ranges for Diagnostically Important Hematological and Blood Chemistry Characteristics of Rainbow Trout (Salmo gairdneri)

1983 ◽  
Vol 40 (4) ◽  
pp. 420-425 ◽  
Author(s):  
W. Roscoe Miller III ◽  
Albert C. Hendricks ◽  
John Cairns Jr.
Keyword(s):  
Rainbow Trout ◽  
Blood Chemistry ◽  
Serum Glucose ◽  
Salmo Gairdneri ◽  
Total Serum Protein ◽  
Total Serum ◽  
Tolerance Interval ◽  
Glucose Levels ◽  
Normal Ranges ◽  
Nonparametric Techniques

Wytheville strain rainbow trout (Salmo gairdneri) were used in an 11-mo study designed to establish normal ranges for several hematological and blood chemistry characteristics. Two nonparametric techniques, percentile estimation and tolerance interval, were used and produced comparable ranges to those based on the Gaussian distribution. Serum glucose levels appeared to coincide with the condition of the gonads; low glucose levels corresponded with approximate spawning times at the hatchery. Total serum protein and gonadal condition were similarly related. High variability of the serum enzymes LDH and SGOT was partially explained by a positive linear relationship between enzyme activity and acclimation temperatures. In addition to physiological significance, determination of normal ranges for rainbow trout has promise in diagnosis of pathological, disease, and toxicant-induced stresses.

Statistical Methods for Estimating Normal Blood Chemistry Ranges and Variance in Rainbow Trout (Salmo gairdneri), Shasta Strain

1975 ◽  
Vol 32 (4) ◽  
pp. 551-554 ◽  
Author(s):  
Gary A. Wedemeyer ◽  
Nancy C. Nelson
Keyword(s):  
Statistical Methods ◽  
Hemoglobin Concentration ◽  
Blood Chemistry ◽  
Inorganic Phosphorus ◽  
Salmo Gairdneri ◽  
Tolerance Interval ◽  
Interval Method ◽  
Normal Ranges ◽  
Estimate Procedure ◽  
Blood Variables

Gaussian and nonparametric (percentile estimate and tolerance interval) statistical methods were used to estimate normal ranges for blood chemistry (bicarbonate, bilirubin, calcium, hematocrit, hemoglobin, magnesium, mean cell hemoglobin concentration, osmolality, inorganic phosphorus, and pH for juvenile rainbow (Salmo gairdneri, Shasta strain) trout held under defined environmental conditions. The percentile estimate and Gaussian methods gave similar normal ranges, whereas the tolerance interval method gave consistently wider ranges for all blood variables except hemoglobin. If the underlying frequency distribution is unknown, the percentile estimate procedure would be the method of choice.

Some Blood Chemistry Values for the Rainbow Trout (Salmo gairdneri)

1970 ◽  
Vol 27 (6) ◽  
pp. 1162-1164 ◽  
Author(s):  
Gary Wedemeyer ◽  
K. Chatterton
Keyword(s):  
Rainbow Trout ◽  
Normal Distribution ◽  
Blood Urea Nitrogen ◽  
Total Protein ◽  
Kidney Tissue ◽  
Blood Chemistry ◽  
Salmo Gairdneri ◽  
Clinical Test ◽  
Urea Nitrogen ◽  
Normal Ranges

Normal distribution curves were graphically fitted to approximately 1400 clinical test values obtained from the plasma or kidney tissue of more than 200 yearling rainbow trout (Salmo gairdneri). Estimated normal ranges were ascorbate, 102–214 μg/g; blood urea nitrogen (BUN), 0.9–4.5 mg/100 ml; chloride, 84–132 mEq/liter; cholesterol, 161–365 mg/100 ml; cortisol, 1.5–18.5 μg/100 ml; glucose, 41–151 mg/100 ml; and total protein, 2–6 g/100 ml.

Glomerular permselectivity to serum proteins in rainbow trout (Salmo gairdneri)

1988 ◽  
Vol 255 (3) ◽  
pp. R418-R423
Author(s):  
B. Elger ◽  
H. Ruhs ◽  
H. Hentschel
Keyword(s):  
Rainbow Trout ◽  
Glomerular Filtration ◽  
Serum Proteins ◽  
Salmo Gairdneri ◽  
Total Serum Protein ◽  
Total Serum ◽  
Protein Patterns ◽  
Gradient Gel Electrophoresis ◽  
Glomerular Hemodynamics

Changes in glomerular filtration rate (GFR) were induced in the unanesthetized rainbow trout by short-term adaptation from freshwater (FW) to brackish water (BW, 50% seawater) to investigate the effect of altered glomerular function on the glomerular permselectivity to endogenous serum proteins in vivo. Protein patterns of serum and urine were obtained by nondenaturing polyacrylamide gradient gel electrophoresis. In the serum, high-molecular-weight proteins dominate by 83.1 +/- 8.16%, and total serum protein concentrations were 3.25 +/- 0.32 (FW) and 3.12 +/- 0.16 g/100 ml (BW). The urinary protein concentration increased from 16.0 +/- 1.41 (FW) to 42.8 +/- 11.0 mg/100 ml (BW), whereas GFR and urine flow rate, both linearly related, decreased from 4.55 +/- 0.34 (FW) to 1.84 +/- 0.11 (BW) ml.h-1.kg-1 and from 2.31 +/- 0.19 (FW) to 0.56 +/- 0.02 (BW) ml.h-1.kg-1, respectively. The fractional clearance of protein increased exponentially at decreased GFR. This suggests increased glomerular filtration of serum proteins due to altered glomerular hemodynamics.

Effects of Water Salinity and Dietary Protein Levels on Total Serum Protein and Hematocrit of Rainbow Trout (Salmo gairdneri) Fingerlings

1974 ◽  
Vol 31 (6) ◽  
pp. 1133-1134 ◽  
Author(s):  
Ibrahim H. Zeitoun ◽  
Duane E. Ullrey ◽  
Peter I. Tack
Keyword(s):  
Rainbow Trout ◽  
Serum Protein ◽  
Dietary Protein ◽  
Water Salinity ◽  
Salmo Gairdneri ◽  
Total Serum Protein ◽  
Total Serum ◽  
Protein Levels ◽  
Salinity Water ◽  
Different Levels

Seven separate diets ranging from 30 to 60% protein in 5% increments were fed for 10 wk to duplicate groups of rainbow trout, Salmo gairdneri, fingerlings maintained at 10 and 20‰ salinity. Water salinity and dietary protein concentration did not significantly influence total serum protein. Hematocrit increased substantially with salinity (35.3% for fish at 10 ppt and 39.1% for those at 20 ppt), whereas different levels of dietary protein were of minor consequence.

Changes in plasma insulin and carbohydrate metabolism of zinc-stressed rainbow trout, Salmo gairdneri

1982 ◽  
Vol 60 (9) ◽  
pp. 2079-2084 ◽  
Author(s):  
G. F. Wagner ◽  
B. A. McKeown
Keyword(s):  
Rainbow Trout ◽  
Pancreatic Beta Cells ◽  
Plasma Insulin ◽  
Zinc Sulphate ◽  
Liver Glycogen ◽  
Salmo Gairdneri ◽  
Control Group ◽  
Exposed Fish ◽  
Glucose Levels ◽  
Insulin Radioimmunoassay

An experiment was conducted to determine if the hyperglycemia that is observed in zinc-stressed fish is also accompanied by changes in the levels of plasma insulin and liver glycogen. Juvenile rainbow trout were exposed to three concentrations of zinc sulphate along with a control group over 31 days. Plasma glucose levels were monitored in each group over the course of the experiment. The group demonstrating the most acute and sustained hyperglycemia (0.352 ppm zinc) was then analyzed along with the controls for changes in plasma insulin (using a teleost insulin radioimmunoassay) and liver glycogen levels. Significant depressions in plasma insulin and liver glycogen levels were observed in the zinc-exposed fish when compared with the controls. These changes are discussed with respect to possible influences of epinephrine, which is elevated in stressed fish, and (or) a direct effect of zinc metal on the pancreatic beta cells.

Determination of Total Serum Protein Levels Fed by Hot Smoked Rainbow Trout (Oncorhynchus mykiss) Diets in Rats

2008 ◽  
Vol 3 (4) ◽  
pp. 246-251
Author(s):  
Suleyman Kaleli ◽  
Mustafa Unlusayin ◽  
Sengul Bilgin ◽  
Levent Izci ◽  
Ali Gunlu
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Serum Protein ◽  
Total Serum Protein ◽  
Total Serum ◽  
Protein Levels ◽  
Rainbow Trout Oncorhynchus Mykiss

Long-Term Sublethal Acid Exposure in Rainbow Trout (Salmo gairdneri) in Soft Water: Effects on Ion Exchanges and Blood Chemistry

1988 ◽  
Vol 45 (8) ◽  
pp. 1387-1398 ◽  
Author(s):  
Céline Audet ◽  
R. Stephen Munger ◽  
Chris M. Wood
Keyword(s):  
Rainbow Trout ◽  
Ammonia Excretion ◽  
Blood Chemistry ◽  
Soft Water ◽  
Acid Exposure ◽  
Salmo Gairdneri ◽  
Plasma Parameters ◽  
Water Effects ◽  
Net Flux

Long-term sublethal acid exposure (3 mo, pH 4.8) in adult rainbow trout (Salmo gairdneri) acclimated to artificial soft water (Ca2+ = 50, Na+ = 50, Cl− = 100 μeq∙L−1) caused transient net fosses of Na+ and Cl−. Net flux rates of both ions were returned to control levels after 30–52 d of acid exposure through a new equilibrium between unidirectional influx and efflux, where both were lower than control rates. K+ balance remained negative and Ca2+ balance at zero throughout the exposure. No changes in net acidic equivalent flux occurred, indicating the absence of acid–base disturbance, but ammonia excretion increased over time. Muscle K+, Na+, and Cl− fell and Ca2+ increased. Plasma Na+, Cl−, and osmolality decreased, while plasma protein, glucose, and blood hemoglobin increased during the first few weeks of acid exposure. Plasma K+ and Ca2+ did not change. General stabilization of plasma parameters occurred in concert with the stabilization of Na+ and Cl− flux rates, but no recovery to control levels was observed for any of them. We conclude that despite this stabilization at a new steady state, rainbow trout were physiologically affected in a deleterious manner by chronic sublethal acid exposure in soft water.

scholarly journals The physiological status and mortality associated with otter-trawl capture, transport, and captivity of an exploited elasmobranch, Squalus acanthias

2006 ◽  
Vol 64 (1) ◽  
pp. 122-130 ◽  
Author(s):  
John W. Mandelman ◽  
Marianne A. Farrington
Keyword(s):  
Blood Chemistry ◽  
Blood Parameters ◽  
Sampling Interval ◽  
Serum Glucose ◽  
Squalus Acanthias ◽  
Otter Trawl ◽  
Physiological Status ◽  
Glucose Levels ◽  
Base Parameters ◽  
Sampling Intervals

Abstract Mandelman, J. M., and Farrington, M. A. 2007. The physiological status and mortality associated with otter-trawl capture, transport, and captivity of an exploited elasmobranch, Squalus acanthias. ICES Journal of Marine Science, 64: 122–130. To assess the physiological responses and associated mortality in spiny dogfish (Squalus acanthias) following capture in an otter trawl and exposure to additional conditions, blood samples were obtained subsequent to three sampling intervals: capture (T1), transport (T2), and captivity (T3). The results indicate that marked differences existed in blood chemistry at each sampling interval. Acid–base parameters (vascular pH, pO2, pCO2), serum Ca2+ and Cl−, and haematocrit were maximally disrupted at T1, but progressively resolved to presumed basal values by T3. Concentrations of whole-blood lactate, plasma total protein, additional sera electrolytes (Na+, K+, Mg2+), and BUN (urea) were maximally compromised at T2, but also recovered by T3. In contrast, serum glucose levels were similar at T1 and T2 but rose to peak levels by T3. Although blood parameters were substantially altered, dogfish mortality was low (2 out of 34; 5.9%), suggesting a strong degree of resilience to compounded stressors associated with capture, transport, and captivity.

scholarly journals Dose–Response Study of Microcystin Congeners MCLA, MCLR, MCLY, MCRR, and MCYR Administered Orally to Mice

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 86
Author(s):  
Neil Chernoff ◽  
Donna Hill ◽  
Johnsie Lang ◽  
Judith Schmid ◽  
Amy Farthing ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Weight Ratio ◽  
Serum Glucose ◽  
Total Serum ◽  
Hepatic Toxicity ◽  
Glucose Levels ◽  
Clinical Changes ◽  
Dose Response Study

Microcystins are common freshwater cyanobacterial toxins that affect liver function. The toxicities of five microcystin congeners (microcystin-LA (MCLA), MCLR, MCLY, MCRR, and MCYR) commonly observed in harmful algal blooms (HABs) were evaluated in BALB/c mice after a single oral administration of doses ranging from those that were no observed adverse effect levels (NOAELs) to lowest observed adverse effect levels (LOAELs). Animals were monitored for changes in behavior and appearance, and euthanized 24 h after dosing. Test endpoints included clinical changes, necropsy observations, and serum indicators of hepatic toxicity and general homeostasis. Doses were 0.5–7 mg/kg MCLA, 0.5–11 mg/kg MCLR, 1–7 mg/kg MCLY, 7–22 mg/kg MCRR, and 3–11 mg/kg MCYR. MCLA at 3 mg/kg elevated liver/body weight ratio and liver score, ALT, AST, and GLDH, indicating hepatic toxicity, reduced serum glucose and highly elevated total serum bilirubin. MCLR and MCLY induced similar effects with LOAELs of 5 mg/kg, although a greater extent and severity of effects were observed in MCLR animals. MCRR exposure at 22 mg/kg was associated with reduced serum glucose. MCYR induced scattered liver effects at 7 mg/kg and reduced serum glucose levels at 5 mg/kg. The results indicate significant differences in congener-induced toxicity after microcystin exposure.

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