Effects of Water Salinity and Dietary Protein Levels on Total Serum Protein and Hematocrit of Rainbow Trout (Salmo gairdneri) Fingerlings

1974 ◽  
Vol 31 (6) ◽  
pp. 1133-1134 ◽  
Author(s):  
Ibrahim H. Zeitoun ◽  
Duane E. Ullrey ◽  
Peter I. Tack
Keyword(s):  
Rainbow Trout ◽  
Serum Protein ◽  
Dietary Protein ◽  
Water Salinity ◽  
Salmo Gairdneri ◽  
Total Serum Protein ◽  
Total Serum ◽  
Protein Levels ◽  
Salinity Water ◽  
Different Levels

Seven separate diets ranging from 30 to 60% protein in 5% increments were fed for 10 wk to duplicate groups of rainbow trout, Salmo gairdneri, fingerlings maintained at 10 and 20‰ salinity. Water salinity and dietary protein concentration did not significantly influence total serum protein. Hematocrit increased substantially with salinity (35.3% for fish at 10 ppt and 39.1% for those at 20 ppt), whereas different levels of dietary protein were of minor consequence.

Effects of sodium fluoride on total serum protein levels and transaminase activity in rats

2002 ◽  
Vol 56 (4) ◽  
pp. 169-172 ◽  
Author(s):  
D Qujeq ◽  
B Laghaie ◽  
A Gholipour ◽  
N Solimani ◽  
S Hassenzadeh
Keyword(s):  
Serum Protein ◽  
Sodium Fluoride ◽  
Total Serum Protein ◽  
Total Serum ◽  
Transaminase Activity ◽  
Protein Levels

Use of two vegetable by-products as protein sources in rainbow trout feeding

1992 ◽  
Vol 55 (2) ◽  
pp. 277-284 ◽  
Author(s):  
F. J. Moyano ◽  
G. Cardenete ◽  
M. de la Higuera
Keyword(s):  
Rainbow Trout ◽  
Dietary Protein ◽  
Fish Meal ◽  
Nutrient Utilization ◽  
Average Weight ◽  
Protein Levels ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Meal Protein ◽  
By Products ◽  
Different Levels

AbstractTwo experiments were designed to test the possibility of partially replacing fish-meal protein in rainbow trout either with maize-gluten meal (MGM) (experiment 1) or potato protein concentrate (PPC) (experiment 2). Rainbow trout (Oncorhynchus mykiss) 30 g initial average weight were given diets containing different levels of MGM or PPC proteins ranging from 0 to 0·4 or to 0·6 of dietary protein, respectively. Substitutions of fish meal either by MGM or PPC were carried out establishing two different total dietary protein levels; 350 and 450 g/kg. Results showed that the MGM diets were acceptable and gave a significant enhancement (over 0·37 in the better case) in nutrient utilization when compared with those including only fish meal. On the contrary, diets including PPC were poorly accepted, and the growth offish and nutrient utilization were negatively correlated with dietary levels of PPC. It is concluded that levels of MGM representing around 400 g/kg diet can be used in foods for rainbow trout, whereas PPC appears not to be a suitable protein source for those fish.

Normal Ranges for Diagnostically Important Hematological and Blood Chemistry Characteristics of Rainbow Trout (Salmo gairdneri)

1983 ◽  
Vol 40 (4) ◽  
pp. 420-425 ◽  
Author(s):  
W. Roscoe Miller III ◽  
Albert C. Hendricks ◽  
John Cairns Jr.
Keyword(s):  
Rainbow Trout ◽  
Blood Chemistry ◽  
Serum Glucose ◽  
Salmo Gairdneri ◽  
Total Serum Protein ◽  
Total Serum ◽  
Tolerance Interval ◽  
Glucose Levels ◽  
Normal Ranges ◽  
Nonparametric Techniques

Wytheville strain rainbow trout (Salmo gairdneri) were used in an 11-mo study designed to establish normal ranges for several hematological and blood chemistry characteristics. Two nonparametric techniques, percentile estimation and tolerance interval, were used and produced comparable ranges to those based on the Gaussian distribution. Serum glucose levels appeared to coincide with the condition of the gonads; low glucose levels corresponded with approximate spawning times at the hatchery. Total serum protein and gonadal condition were similarly related. High variability of the serum enzymes LDH and SGOT was partially explained by a positive linear relationship between enzyme activity and acclimation temperatures. In addition to physiological significance, determination of normal ranges for rainbow trout has promise in diagnosis of pathological, disease, and toxicant-induced stresses.

Glomerular permselectivity to serum proteins in rainbow trout (Salmo gairdneri)

1988 ◽  
Vol 255 (3) ◽  
pp. R418-R423
Author(s):  
B. Elger ◽  
H. Ruhs ◽  
H. Hentschel
Keyword(s):  
Rainbow Trout ◽  
Glomerular Filtration ◽  
Serum Proteins ◽  
Salmo Gairdneri ◽  
Total Serum Protein ◽  
Total Serum ◽  
Protein Patterns ◽  
Gradient Gel Electrophoresis ◽  
Glomerular Hemodynamics

Changes in glomerular filtration rate (GFR) were induced in the unanesthetized rainbow trout by short-term adaptation from freshwater (FW) to brackish water (BW, 50% seawater) to investigate the effect of altered glomerular function on the glomerular permselectivity to endogenous serum proteins in vivo. Protein patterns of serum and urine were obtained by nondenaturing polyacrylamide gradient gel electrophoresis. In the serum, high-molecular-weight proteins dominate by 83.1 +/- 8.16%, and total serum protein concentrations were 3.25 +/- 0.32 (FW) and 3.12 +/- 0.16 g/100 ml (BW). The urinary protein concentration increased from 16.0 +/- 1.41 (FW) to 42.8 +/- 11.0 mg/100 ml (BW), whereas GFR and urine flow rate, both linearly related, decreased from 4.55 +/- 0.34 (FW) to 1.84 +/- 0.11 (BW) ml.h-1.kg-1 and from 2.31 +/- 0.19 (FW) to 0.56 +/- 0.02 (BW) ml.h-1.kg-1, respectively. The fractional clearance of protein increased exponentially at decreased GFR. This suggests increased glomerular filtration of serum proteins due to altered glomerular hemodynamics.

THE CATABOLISM OF 131I-LABELLED HOMOLOGOUS γ-GLOBULIN IN NORMAL, HYPERTHYROID AND HYPOTHYROID RATS

1960 ◽  
Vol 21 (1) ◽  
pp. 83-89 ◽  
Author(s):  
C. P. FARTHING ◽  
JULIA GERWING ◽  
JENNIFER SHEWELL
Keyword(s):  
Serum Protein ◽  
Half Life ◽  
Total Serum Protein ◽  
Total Serum ◽  
Protein Levels ◽  
Globulin Level ◽  
Thyroxine Treatment ◽  
Biological Half Life ◽  
Catabolic Rate

SUMMARY The biological half-life of injected homologous 131I-γ-globulin was found to be 4·8 days in euthyroid, 5·8 days in thyroidectomized, and 3·8 days in thyroxine-treated rats. The catabolic rate of homologous γ-globulin, expressed as a percentage of the intravascular protein broken down per day, was decreased from 25·5% in normal rats to 22·5% in thyroidectomized animals, and increased to 39·2% by thyroxine treatment. Total serum protein levels were not affected by thyroxine treatment (0·02 mg/kg) in the rat, but increased after thyroidectomy. This increase was chiefly due to an increased γ-globulin level, and it is postulated that catabolism of γ-globulin in the thyroidectomized rat is decreased to a greater extent than is γ-globulin synthesis.

EFFECTS OF VARYING DIETARY ENERGY, PROTEIN AND AMINO ACID LEVELS ON GROWTH, NUTRIENT DIGESTIBILITY, AND SERUM PROTEIN LEVELS OF EARLY WEANED PIGS

1967 ◽  
Vol 47 (1) ◽  
pp. 77-83 ◽  
Author(s):  
J. F. Standish ◽  
J. P. Bowland
Keyword(s):  
Amino Acid ◽  
Serum Protein ◽  
Feed Intake ◽  
Crude Protein ◽  
Feed Utilization ◽  
Nutrient Digestibility ◽  
Total Serum Protein ◽  
Total Serum ◽  
Or Efficiency ◽  
Protein Levels

Fifteen rations formulated by a factorial arrangement of 3 fat and 5 protein treatments were fed to non-castrate crossbred pigs from 3 to 9 weeks of age. Levels of 0, 4, and 8% fat and 12, 16, and 20% crude protein, and 12 and 16% crude protein with supplemental lysine and methionine to equal that present in the 20% protein diet were fed. Each increment of protein resulted in increased rate of gain. The amino acid supplemented 16% protein ration supported as rapid gain and as efficient utilization of feed as did the 20% ration. Feed intake and efficiency of feed utilization were improved as protein was raised from 12 to 16% and when lysine and methionine were added to the rations. Fat levels were not found to affect rate of gain, feed intake or efficiency of feed utilization. Apparent digestibility of nitrogen was higher in the 20% protein ration than the 12 or 16% protein rations and was also found to increase when amino acids were added to the low protein rations. More of the apparent digestible nitrogen was retained from the amino acid-supplemented rations. Increasing fat levels were found to reduce: the total serum protein levels in gilts but not in boars.

scholarly journals Selected haematological changes in Clarias gariepinus (Burchell, 1822) infected with a Trypansosoma sp. from the Vaal Dam, South Africa

2013 ◽  
Vol 80 (1) ◽  
Author(s):  
Maryke L. Ferreira ◽  
Annemarie Avenant-Oldewage
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Clarias Gariepinus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Total Plasma ◽  
Breeding Stock ◽  
Protein Levels ◽  
Total Plasma Protein ◽  
Haematological Changes

The use of haematological techniques to assess fish health is generally accepted. The aim of the current study was to determine selected haematological changes that occur in Clarias gariepinus (Burchell, 1822). infected with trypanosomes. Blood films were prepared according to standard techniques to confirm trypanosome infections and whole blood was collected, the serum and plasma separated, and prepared for albumin and total protein concentration analysis. Plasma albumin levels were significantly higher in infected wild caught fish than in uninfected wild caught fish and uninfected breeding stock. Serum albumin levels were significantly lower in infected wild caught fish when compared to uninfected breeding stock. The total plasma and serum protein levels were within the normal range for C. gariepinus, that is, 3 g – 6 g/100 mL. The total plasma protein levels varied significantly between the three groups. However, the total serum protein levels were only significantly different between uninfected breeding stock and uninfected wild caught fish, as well as uninfected breeding stock and infected wild caught fish. The total protein levels were significantly higher in infected wild caught fish than in the other groups, a possible indication of an infection or inflammatory host response.

The effect of different levels of crude protein on the pathophysiology of Haemonchus contortus infection in 2-year-old Boer dry does under confined conditions

2017 ◽  
Vol 57 (4) ◽  
pp. 719
Author(s):  
T. V. Can ◽  
M. A. Hohenhaus ◽  
P. J. Murray
Keyword(s):  
Serum Protein ◽  
Dietary Protein ◽  
Haemonchus Contortus ◽  
Haemoglobin Concentration ◽  
Basal Diet ◽  
Protein Supplementation ◽  
Total Serum Protein ◽  
Total Serum ◽  
Control Group ◽  
Cell Volumes

The effect of dietary protein supplementation on the pathophysiology of a single Haemonchus contortus infection in 2-year-old Boer dry does under confined conditions was examined. Twenty-four does were randomly confined in individual pens and allocated into three groups balanced by their liveweight. The goats in the Control group were offered a basal diet whereas goats in Treatments 1 and 2 were fed the basal diet plus 25% and 50% dietary protein increments, respectively. Each animal was orally administrated 100 H. contortus L3 larvae/kg liveweight on Day 0 of the experiment. The goat liveweights and FAMACHA scores were recorded at weekly intervals. Packed cell volumes, eosinophil percentage, haemoglobin concentration, total serum protein, globulin, and albumin concentrations were recorded on Days 0 and 14, and then at weekly intervals during the experiments. Faecal egg counts were measured on Days 0, 21, and then at weekly intervals thereafter. Antigen-specific IgA, IgG, and IgM titres were determined from Enzyme-linked immunosorbent assays tests on Days 0, 28, and at the termination of the experiment. The results of this study showed that a single H. contortus L3 infection did not cause pathogenic effects in 2-year-old Boer dry does. Dietary protein supplementation significantly lowered faecal egg count and significantly enhanced IgG titres (P < 0.05). However, the higher protein diets did not have any significant effects on liveweight, packed cell volumes, haemoglobin concentration, biological parameters (total serum protein, albumin, and globulin concentrations), and peripheral antibodies (IgA and IgM titres) between the treatments. Further investigations are required to clarify the role of protein supplementation to control H. contortus infection in goats.

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