STUDIES ON THE SAL LOCUS IN DROSOPHILA PSEUDOOBSCURA. III. THE MOLECULAR PATTERN OF DNA IN ACTIVE AND INACTIVE CHROMOSOME REGIONS
The banding pattern at the tip of salivary gland chromosome 3 in Drosophila pseudoobscura is determined by a nearby locus, sal (salivary). A number of cytological changes in the neighborhood of the sal locus have been obtained. Their effects on the appearance of bands are interpreted in terms of the fine structure of the chromosome and the arrangement of the DNA double helix. Bands as viewed in the light microscope may become dispersed, as in puffs, or compacted, as in position effects involving their transposition to heterochromatic regions. Changes of bands are interpreted in terms of dispersion or compaction of DNA.Secondary puffing is induced in a paired sal chromosome which does not puff when unpaired, by a sal+ homologue which puffs when unpaired. This is explained as being due to osmotic effects caused by added protein and gene products (RNAs) diffusing from the sal+ to the sal chromosome, when they are paired, through submicroscopic interconnecting spaces within the chromosomes.