INDUCTION OF MUTATIONS BY CHEMICALS AND GAMMA RAYS IN MUTANTS OF YEAST REFRACTORY TO UV-MUTAGENESIS

1977 ◽  
Vol 19 (2) ◽  
pp. 323-330 ◽  
Author(s):  
A. Nasim ◽  
M. A. Hannan

Radiation-sensitive mutants of Schizosaccharomyces pombe, known to be refractory to UV-mutagenesis, were tested for mutability caused by treatments with chemicals and gamma rays. One such mutant (rad3) was studied over a wide range of UV doses to compare the kinetics of its mutational response to that of the wild type. All such comparisons were carried out using a forward mutation system. Data show that, unlike UV, the chemical mutagens as well as gamma rays produced mutations (although at reduced frequency), in the strains of S. pombe tested, indicating the existence of an additional mechanism(s) for chemical and gamma ray induced mutations. These observations are discussed as these relate to the pathways for repair of mutational damage in yeast.

1998 ◽  
Vol 188 ◽  
pp. 159-162
Author(s):  
Gerald J. Fishman

Gamma-ray bursts (GRBs) will be recorded as one of the outstanding new phenomena discovered in astronomy this century. About once per day, a burst of gamma rays appears from a random direction on the sky. Often, the burst outshines all other sources of gamma-rays in the sky, combined. This paper reviews some of the key observed phenomenon of bursts in the hard x-ray/gamma-ray region, as observed with the BATSE experiment on the Compton Gamma Ray Observatory. The observed time profiles, spectral properties and durations of gamma-ray bursts cover a wide range. Recent breakthroughs in the observation of gamma-ray burst counterparts and afterglows in other wavelength regions have marked the beginning of a new era in gamma-ray burst research. Those observations are described in following papers in these proceedings.


2019 ◽  
Vol 48 (6) ◽  
pp. 540-546 ◽  
Author(s):  
Sameh Mohamed Gafar ◽  
Nehad Magdy Abdel-Kader

Purpose The purpose of this paper is to study the effect of gamma-rays on murexide (Mx) dye and its possible use as radiation dosimeters in two different dosimetry systems. The first system depends on the Mx dye as a liquid dosimeter. The second dosimetry system depends also on the same dye but as in a gel form, which is more sensitive to gamma-rays. Design/methodology/approach The prepared Mx (solutions/gels) have a considerable two peaks at 324 and 521 nm that upon irradiation, the intensity of these peaks decreases with the increasing radiation dose. Findings The gamma-ray absorbed dose for these dosimeters was found to be up to 2 kGy for the solution samples and 40 Gy for the gels. Radiation chemical yield, dose response function, radiation sensitivity and before and after-irradiation stability under various conditions were discussed and studied. Practical implications It is expected that the radiolysis of the Mx dye can be used as radiation dosimeters in two different dosimetry systems; liquid and gel dosimeters. This can be applied in a wide range of gamma radiation practical industrial applications in water treatment, food irradiation dosimeters, radiotherapy and fresh food irradiation and seed production. Originality/value Both of the prepared Mx dyes, either as solutions or gel samples, can be facilely prepared from commercially, cheap, safe, available chemicals and suitable for useful applied Mx solutions and gels radiation dosimeters.


2004 ◽  
Vol 186 (9) ◽  
pp. 2673-2681 ◽  
Author(s):  
Christopher D. Herring ◽  
Frederick R. Blattner

ABSTRACT The essential genes of microorganisms encode biological functions important for survival and thus tend to be of high scientific interest. Drugs that interfere with essential functions are likely to be interesting candidates for antimicrobials. However, these genes are hard to study genetically because knockout mutations in them are by definition inviable. We recently described a conditional mutation system in Escherichia coli that uses a plasmid to produce an amber suppressor tRNA regulated by the arabinose promoter. This suppressor was used here in the construction of amber mutations in seven essential E. coli genes. Amber stop codons were introduced as “tagalong” mutations in the flanking DNA of a downstream antibiotic resistance marker by lambda red recombination. The drug marker was removed by expression of I-SceI meganuclease, leaving a markerless mutation. We demonstrate the method with the genes frr, gcpE, lpxC, map, murA, ppa, and rpsA. We were unable to isolate an amber mutation in ftsZ. Kinetics of cell death and morphological changes were measured following removal of arabinose. As expected given the wide range of cellular mechanisms represented, different mutants showed widely different death curves. All of the mutations were bactericidal except the mutation in gcpE, which was bacteriostatic. The strain carrying an amber mutation in murA was by far the most sensitive, showing rapid killing in nonpermissive medium. The MurA protein is critical for peptidoglycan synthesis and is the target for the antibiotic fosfomycin. Such experiments may inexpensively provide valuable information for the identification and prioritization of targets for antibiotic development.


2021 ◽  
pp. 24-39
Author(s):  
Hitoshi Nakagawa

Abstract Following the construction of the Gamma Field at the Institute of Radiation Breeding in 1960, mutation breeding was accelerated in Japan. The facility is used, with a radiation dose up to 2 Gy/day (ca. 300,000 times that of natural background), to induce mutations at a higher frequency than occurs in nature. There have been 318 direct- use mutant cultivars representing 79 species generated through irradiation of gamma-rays, X-rays, ion beams and chemicals and somaclonal variation. Approximately 79% of these direct-use cultivars were induced by radiation. There have been 375 indirect-use mutant cultivars, including 332 rice, of which 162 cultivars (48.8%) were derived from the semi-dwarf mutant cv. 'Reimei'. The economic impact of these mutant cultivars, primarily of rice and soybean, is very large. Some useful mutations are discussed for rice, such as low digestible protein content, low amylose content, giant embryo and non-shattering. Useful mutations in soybean such as radiosensitivity, fatty acid composition and super-nodulation have been identified. Japanese pear and apple resistant to Alternaria disease have also been identified. The achievements of biological research such as characterization and determination of deletion size generated by gamma-rays, the effect of deletion size and the location, and a mechanism of dominant mutation induction are identified. Similarly, genetic studies on mutations generated through the use of gamma-ray induced mutations, such as phytochrome response, aluminium tolerance, stay-green (Mendel's gene) and epicuticular wax have also been conducted. Mutation breeding is a very useful technology for isolating genes and for elucidating gene functions and metabolic pathways in various crops.


2017 ◽  
Vol 64 ◽  
pp. 17-31 ◽  
Author(s):  
Rafiul Amin Laskar ◽  
Samiullah Khan

In mutation breeding, mutagenic effectiveness and efficiency are the base parameters to predict the mutagenic potency of any mutagen. Studies on mutagenic effectiveness and mutagenic efficiency of physical mutagen (gamma rays) and chemical mutagen (hydrazine hydrates; HZ) on two cultivars of lentil (Lens culinaris Medik.), viz. DPL 62 (macrosperma) and Pant L 406 (microsperma) have been reported. Dry and healthy seeds were treated with four doses of each gamma rays (100-400 Gy), HZ (0.1-0.4 %) and their combinations. Frequencies of the induced agro-morphological variations into different phenotypic categories were estimated in M2 population that resulted into identification and isolation of wide range of mutants with altered phenotypes. Data on effectiveness and efficiency of various mutagenic treatments calculated on the basis of the frequency of chlorophyll mutations, which showed effectiveness and efficiency were higher at the moderate doses of gamma rays and HZ, while in case of combination treatments; lower doses were most effective and efficient with few inter-varietal exceptions. Phenotyping of the mutants revealed that growth habits was the most sensitive category to which most of the mutant belongs, followed by leaf and flower/pod/seed in both the cultivars studied. Overall, the screened and isolated mutants with economically important agronomic traits can be further propagated in the subsequent generation for development of elite lentil mutant cultivars.


1983 ◽  
Vol 23 (03) ◽  
pp. 553-564 ◽  
Author(s):  
Peter Westaway ◽  
Russel Hertzog ◽  
Ronald E. Plasek

The weighted least-squares (WLS) approach to spectral analysis has enabled more information to be extracted from the downhole recorded induced gamma ray spectra than was previously possible. GST (gamma ray spectroscopy tool), with its optimized inelastic and capture spectral modes, permits analysis of most and often all significant elements present in the formation and provides the possibility of evaluating hydrocarbons, salinity, lithology, porosity, and shaliness. Data have been obtained in a wide range of conditions in open and cased holes with the GST tool both in its present and experimental versions. This paper presents field examples to demonstrate the versatility and potential of the technique, not only as an effective oil-finder independent of water salinity conditions but as a valuable input to a more complete interpretation of well logs. Introduction Nuclear well logging has been long established as a means of evaluating reservoir porosity and hydrocarbons in open hole and behind casing. The count rates of neutrons or gamma rays returning to one or more detectors are measured and related to the formation rock characteristics according to the physics of the neutron inter-actions that have occurred. For example, high-energy neutrons interact with the surrounding formation nuclei and can induce gamma ray emission. Most conventional neutron/gamma spectroscopy techniques for well logging that have been developed to date are based on integral counts in rather broad energy windows. In this paper, we discuss an alternate technique that allows an accurate and detailed formation evaluation. Gamma rays emitted from the formation nuclei are limited to specific and well-defined energies governed by the laws of quantum mechanics. Each element (isotope) has a characteristic spectrum of gamma rays that can be emitted from a given neutron interaction. Therefore, an element may be identified by its gamma my spectral shape or signature whose emission intensity is related to the elemental concentration. The GST tool measures the relative yields of gamma rays resulting from the interactions of neutrons with different elements present in the formation. The measurements are based on a WLS shape analysis of the observed gamma ray spectral distribution. This is a recently introduced approach to induced nuclear logging. Neutron induced gamma rays are analyzed downhole in terms of intensity in each of more than 200 discrete, narrow energy increments. From this detailed measurement of formation spectral response to neutron bombardment, eight constituent elements can be identified and their proportions estimated. These elements, C, 0, C1, H. Si, Ca, Fe. and S, are significant in formation mineralogical and fluid analysis. A considerable amount of new information is thus made available in the form of a continuous or quasicontinuous well log for a more comprehensive evaluation of the formation. Because of its immediate commercial interest, emphasis in a previous publications was placed on the application of the carbon and oxygen measurements in estimating hydrocarbon saturation. This approach has the advantage of being unaffected by the presence of salts (particularly NACl) in the pore fluid, and has had encouraging success in the monitoring of reservoirs where salinities were either unknown, variable, or too low for conventional neutron logging. SPEJ P. 553^


2017 ◽  
Vol 13 (2) ◽  
pp. 115
Author(s):  
Mukh Syaifudin ◽  
Nila Dariska Adha ◽  
Sofiati Purnami ◽  
Sasmito Wulyoadi ◽  
Edy Marwanto ◽  
...  

Garlic (Allium sativum) contains a wide range of phytocompounds that produce various responses in human body. However the knowledge on the potential of fermented form of garlic in protecting negative impacts of radiation is very limited. In this research in vitro efficacy of fermented garlic in protecting negative impact of gamma ray was studied using cytogenetic test. A set of culture of human lymphocytes was irradiated with 60Co gamma rays at dose of 2 Gy (dose rate of 2 Gy/min) and fermented garlic extract at four working concentrations of 0, 125, 250 and 500 mg/mL were added to these cells and then were incubated at 37oC for 48 hrs. Colcemid was added at 3 hr before harvest to collect metaphase cells and it was done by standard methodology for cytogenetic analysis. The fermented garlic extract significantly (p<0.05) did not exhibited antigenotoxic effect of gamma rays and its effectiveness was same as in control (without extract treatment) group. In contrary all concentration of chemicals (125, 250 and 500 mg/mL) were seemingly tend to induce higher number of dicentric and fragment chromosomes than control under microscopic observation. Mitotic index of the cell that was determined with programmed metaphase finder also did not influenced by garlic addition. It was concluded that aqueous garlic extract did not possesses its efficacy in protecting impact of ionizing radiation.


A comprehensive view of various techniques involved in detection of gamma rays, obtained from literatures has been presented. It also discusses with the current research activities carried out at various fronts, provides information about wide range of dosimeter available for detection of gamma rays. The dose range of each dosimeter is reviewed along with merits and demerits of various dosimeter techniques. Different types of sensing materials and their characteristics are discussed, with relative merits and demerits. The development of various types of optical fibres and their working principles are analyzed. The advantages of fibre Bragg grating over other fibres are also presented


2020 ◽  
Vol 637 ◽  
pp. A86
Author(s):  
◽  
V. A. Acciari ◽  
S. Ansoldi ◽  
L. A. Antonelli ◽  
A. Babić ◽  
...  

Context. Markarian 501 (Mrk 501) is a very high-energy (VHE) gamma-ray blazar located at z = 0.034, which is regularly monitored by a wide range of multi-wavelength instruments, from radio to VHE gamma rays. During a period of almost two weeks in July 2014, the highest X-ray activity of Mrk 501 was observed in ∼14 years of operation of the Neil Gehrels Swift Gamma-ray Burst Observatory. Aims. We characterize the broadband variability of Mrk 501 from radio to VHE gamma rays during the most extreme X-ray activity measured in the last 14 years, and evaluate whether it can be interpreted within theoretical scenarios widely used to explain the broadband emission from blazars. Methods. The emission of Mrk 501 was measured at radio with Metsähovi, at optical–UV with KVA and Swift/UVOT, at X-ray with Swift/XRT and Swift/BAT, at gamma ray with Fermi-LAT, and at VHE gamma rays with the FACT and MAGIC telescopes. The multi-band variability and correlations were quantified, and the broadband spectral energy distributions (SEDs) were compared with predictions from theoretical models. Results. The VHE emission of Mrk 501 was found to be elevated during the X-ray outburst, with a gamma-ray flux above 0.15 TeV varying from ∼0.5 to ∼2 times the Crab nebula flux. The X-ray and VHE emission both varied on timescales of 1 day and were found to be correlated. We measured a general increase in the fractional variability with energy, with the VHE variability being twice as large as the X-ray variability. The temporal evolution of the most prominent and variable segments of the SED, characterized on a day-by-day basis from 2014 July 16 to 2014 July 31, is described with a one-zone synchrotron self-Compton model with variations in the break energy of the electron energy distribution (EED), and with some adjustments in the magnetic field strength and spectral shape of the EED. These results suggest that the main flux variations during this extreme X-ray outburst are produced by the acceleration and the cooling of the high-energy electrons. A narrow feature at ∼3 TeV was observed in the VHE spectrum measured on 2014 July 19 (MJD 56857.98), which is the day with the highest X-ray flux (>0.3 keV) measured during the entire Swift mission. This feature is inconsistent with the classical analytic functions to describe the measured VHE spectra (power law, log-parabola, and log-parabola with exponential cutoff) at more than 3σ. A fit with a log-parabola plus a narrow component is preferred over the fit with a single log-parabola at more than 4σ, and a dedicated Monte Carlo simulation estimated the significance of this extra component to be larger than 3σ. Under the assumption that this VHE spectral feature is real, we show that it can be reproduced with three distinct theoretical scenarios: (a) a pileup in the EED due to stochastic acceleration; (b) a structured jet with two-SSC emitting regions, with one region dominated by an extremely narrow EED; and (c) an emission from an IC pair cascade induced by electrons accelerated in a magnetospheric vacuum gap, in addition to the SSC emission from a more conventional region along the jet of Mrk 501.


2018 ◽  
Vol 180 ◽  
pp. 02123 ◽  
Author(s):  
Marcin Zych ◽  
Robert Hanus ◽  
Marek Jaszczur ◽  
Dariusz Świsulski ◽  
Leszek Petryka ◽  
...  

The rapid development of tomography methods particularly electrical, X and gamma rays allows for a wide range of the information about flow structure. However, all of such methods are quite complicated. At the same time much simpler systems as the measuring system of gamma rays absorption, allows to obtain a all key flow information which describe the two-phase flow. In the article the results of analyzes of radiometric signal that not only allow to recognize the type of flow, but also the assessment of forming structures are presented. Calculation and interpretation of the data were based on the crosscorrelation and cross-spectral density function. In order to verify the calculations the photographic documentation made during the measurements was used.


Sign in / Sign up

Export Citation Format

Share Document