LACTIC DEHYDROGENASE STUDIES IN ANIMALS INFECTED WITH TYPE 12 STREPTOCOCCI OR TREATED WITH STREPTOCOCCAL NEPHROTOXIN

1967 ◽  
Vol 13 (8) ◽  
pp. 1027-1032
Author(s):  
S. C. Elliott ◽  
C. K. Williamson
Keyword(s):  
New Zealand ◽  
Streptococcus Pyogenes ◽  
Wistar Rats ◽  
Marked Decrease ◽  
Biochemical Study ◽  
Lactic Dehydrogenase ◽  
Renal Tissue ◽  
Ldh Activity ◽  
Increased Activity ◽  
New Zealand Rabbits

A histochemical and biochemical study of the reactions of Wistar rats and New Zealand rabbits to Streptococcus pyogenes, type 12, strain H-8, and the nephrotoxin (NT) elaborated by these organisms is presented. A significant lactic dehydrogenase (LDH) increase was found in the sera of all test rats 27 days following treatment with streptococcal NT. However, before this increased activity was detected, a marked decrease in LDH activity was obtained as early as 1 day after the last NT injection. This decrease was modest in rats treated with 1.0 mg NT, but it was great when the dosage of NT was increased to 5.0 mg. Serum LDH values also decreased progressively, up to about 2 weeks, in rabbits infected with nephritogenic streptococci or treated with streptococcal nephrotoxin. Such serum LDH fluctuations did not occur in animals treated with saline or Todd–Hewitt broth. Histochemical studies also suggested an initial inhibition by NT of LDH activity in renal tissue.

scholarly journals COMPARATIVE STUDIES OF THE ISOZYMES OF LACTIC DEHYDROGENASE IN RABBIT AND MAN

1962 ◽  
Vol 116 (5) ◽  
pp. 797-806 ◽  
Author(s):  
Elliot S. Vesell ◽  
John Philip ◽  
Alexander G. Bearn
Keyword(s):  
Tissue Culture ◽  
Lactic Dehydrogenase ◽  
Isozyme Pattern ◽  
Ldh Activity ◽  
Starch Gel ◽  
Species Specific ◽  
Increased Activity ◽  
Rabbit Tissues

During development of rabbit tissues, characteristic sequential alterations in the LDH isozyme pattern occur, and consist for liver and muscle in loss of the most rapidly migrating anodal bands, and increased activity in the cathodal bands and slower migrating anodal bands. In heart the reverse changes were observed. Comparison of the isozyme patterns observed in various fetal and adult human tissues suggests that these same sequential alterations probably occur. A species-specific isozyme pattern is obtained in long term culture of rabbit, chick, and human cells. The alterations in tissue culture are characterized by a gradual redistribution of total LDH activity in which there is decreased intensity of rapidly migrating anodal bands. These sequential alterations are independent of the organ of origin. The number of bands observed in the starch gel is partly dependent upon the total activities applied. Isozymes may provide a convenient method for determining the species of origin of cell lines in common use and for investigating the effects of various alterations in the in vitro environment on cells grown in tissue culture.

LEUCOCYTE CULTURES OF IMMUNIZED RATS AND RABBITS

1970 ◽  
Vol 12 (1) ◽  
pp. 87-89 ◽  
Author(s):  
M. Ray
Keyword(s):  
New Zealand ◽  
Wistar Rats ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Test Animal ◽  
Feasible Method ◽  
Cytogenetic Studies ◽  
The Sacrifice ◽  
New Zealand Rabbits

Leucocytes of Wistar rats and white New Zealand rabbits were cultured following immunization with each of the ABO blood sera as well as with fetal calf serum. The rates of survival, transformation and mitosis varied with the type of agent used for immunization. Both macro- and micromethods were successful. The small amount of blood required by the micromethod makes it a feasible method for repetition of cytogenetic studies because it does not necessitate the sacrifice of the test animal.

Antiurolithiatic effect of Cymbopogon Proximus, Alhagi Maurorum, on Sulfadimidine induced urolithiasis in male New Zealand rabbits

2019 ◽  
Vol 20 (1) ◽  
pp. 12-18
Author(s):  
Sameh El-Nabtity
Keyword(s):  
New Zealand ◽  
Intramuscular Injection ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Blood And Urine Samples ◽  
Group 2 ◽  
New Zealand Rabbits ◽  
Group 1

The present study aimed to investigate the prophylactic effect of Cymbopogon proximus and Alhagi maurorum on Sulfadimidine induced urolithiasis in rabbits . Thirty New Zealand male rabbits were allocated into six equal groups (each of five): Group (1) was used as a negative control. Group(2) were administered sulfadimidine (200mg/kg) by intramuscular injection.Groups(3) and (4) were administered sulfadimidine(200mg/kg) by intramuscular injection and 330mg/kg of Cymbopogon proximus alcoholic and aqueous extracts respectively orally.Groups(5) and (6) were administered sulfadimidine(200mg/kg) by intramuscular injection and 400mg/kg of Alhagi maurorum alcoholic and aqueous extracts respectively orally. The period of experiment was 10 days. Blood and urine samples were collected from rabbits on the 10th day. The results recorded a significant decrease in serum creatinine, urea, uric acid and crystalluria in Cymbopogon proximus and Alhagi maurorum groups compared to sulfadimidine treated group.We conclude that Cymbopogon proximus and Alhagi maurorum have a nephroprotective and antiurolithiatic effects against sulfadimidine induced crystalluria.

The effect of programmed cryopreservation on immunogenicity of bladder mucosa in New Zealand rabbits

Cryobiology ◽  
2012 ◽  
Vol 64 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Yong Lu ◽  
Bingkun Li ◽  
Xiang Wang ◽  
Jie Zheng ◽  
Jinxian Pu ◽  
...  
Keyword(s):  
New Zealand ◽  
Bladder Mucosa ◽  
New Zealand Rabbits

Plasma bioactive LH and testosterone profiles in male New Zealand rabbits experimentally infected with Schistosoma mansoni

Acta Tropica ◽  
2004 ◽  
Vol 92 (3) ◽  
pp. 165-172 ◽  
Author(s):  
Y.S. Kasilima ◽  
E.O. Wango ◽  
C.S. Kigondu ◽  
B.M. Mutayoba ◽  
M. Nyindo
Keyword(s):  
New Zealand ◽  
Schistosoma Mansoni ◽  
New Zealand Rabbits

scholarly journals A Comparative Study of Bioartificial Bone Tissue Poly-L-lactic Acid/Polycaprolactone and PLLA Scaffolds Applied in Bone Regeneration

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Weizong Weng ◽  
Shaojun Song ◽  
Liehu Cao ◽  
Xiao Chen ◽  
Yuanqi Cai ◽  
...  
Keyword(s):  
Lactic Acid ◽  
New Zealand ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Defect ◽  
Mineral Density ◽  
Group A ◽  
Repair Bone Defect ◽  
Group B ◽  
New Zealand Rabbits

Bioartificial bone tissue engineering is an increasingly popular technique to repair bone defect caused by injury or disease. This study aimed to investigate the feasibility of PLLA/PCL (poly-L-lactic acid/polycaprolactone) by a comparison study of PLLA/PCL and PLLA scaffolds applied in bone regeneration. Thirty healthy mature New Zealand rabbits on which 15 mm distal ulna defect model had been established were selected and then were divided into three groups randomly: group A (repaired with PLLA scaffold), group B (repaired with PLLA/PCL scaffold), and group C (no scaffold) to evaluate the bone-remodeling ability of the implants. Micro-CT examination revealed the prime bone regeneration ability of group B in three groups. Bone mineral density of surgical site in group B was higher than group A but lower than group C. Meanwhile, the bone regeneration in both groups A and B proceeded with signs of inflammation for the initial fast degradation of scaffolds. As a whole, PLLA/PCL scaffoldsin vivoinitially degrade fast and were better suited to repair bone defect than PLLA in New Zealand rabbits. Furthermore, for the low mineral density of new bone and rapid degradation of the scaffolds, more researches were necessary to optimize the composite for bone regeneration.

Ameliorative effect of fermented Pentaclethra macrophylla (African oil bean seed) on high fat diet and sucrose drink induced metabolic syndrome in male New Zealand rabbits.

2019 ◽  
Vol 5 (1) ◽  
pp. 42-48
Author(s):  
Nwozo Onyenibe ◽  
Daramola Victoria ◽  
Nwawuba Udogadi
Keyword(s):  
Metabolic Syndrome ◽  
New Zealand ◽  
Normal Control ◽  
High Fat Diet ◽  
Bean Seed ◽  
High Fat ◽  
Treatment And Prevention ◽  
Elevated Glucose ◽  
Ameliorative Effect ◽  
New Zealand Rabbits

The consumption of nutraceuticals has become progressively popular in improving health, as well as disease treatment and prevention. The aim of this study is to evaluate the ameliorative effect of fermented Pentaclethra macrophylla (African oil bean) seed on high fat diet and sucrose drink induced metabolic syndrome in male New Zealand rabbits. All experimental procedures were carried using standard methods. A total of twenty (20) rabbits were used for this study, the animals were randomly grouped into five (5) groups with four (4) animals each. Metabolic syndrome risk factors obesity, hypertension, dyslipidemia and hyperglycemia were determined. HFD+30%SUC had over 1500g body weight (bwt) and fasting blood sugar (FBS) 239.50±5.74 mg/dl  relative to the normal control with 1000±6.73g and 77.00±5.94 respectively whereas ND+30%SUC had lower FBS (l172.50±2.52) compared to HFD+30%SUC. There was a significant p<0.05 increase in body weights in treated groups with 1274±11.19g, 1282.75±4.99g, 1285±4.76g and FBS levels in mg/dL198.25±4.79, 198.75±8.06, 200±3.74 in rabbits  compared to untreated HFD+30%SUC. Glycated hemoglobin, and ?-Amylase were significantly p<0.05 elevated, glucose-6-phosphate dehydrogenase levels were significantly p<0.05 reduced, lipid profile markers (HDL, TRIG & CHOL), electrolytes (sodium, potassium, calcium), urea and creatinine levels were significantly p<0.05 altered in HFD+30%SUC exposed group relative to the normal control and ND+30%SUC. However, dietary supplementation with fermented Pentaclethra macrophylla seeds caused weight, FBS, serum lipid reduction and potentiated electrolyte, urea and creatinine levels. Therefore, according to our results, fermented Pentaclethra macrophylla seed is an excellent nutraceutical and its consumption should be encouraged in modifying high fat and elevated intake of sucrose in rabbit

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