Specificity of the microimmunofluorescence assay for the serodiagnosis of Chlamydia pneumoniae infections

1992 ◽  
Vol 38 (11) ◽  
pp. 1185-1189 ◽  
Author(s):  
Gérard Ozanne ◽  
Johanne Lefebvre
Keyword(s):  
Chlamydia Trachomatis ◽  
Key Words ◽  
Chlamydia Pneumoniae ◽  
Species Specificity ◽  
Chlamydia Psittaci ◽  
Serological Evidence ◽  
Recent Infection ◽  
Cross Reactions ◽  
Chlamydial Species ◽  
Microimmunofluorescence Test

Chlamydia pneumoniae infections are mostly confirmed using an indirect microimmunofluorescence test for which potential cross-reactions between antigens from different chlamydial species are not well documented. Using this assay, 928 sera (507 subjects) submitted for Chlamydia pneumoniae serology were tested for specific IgM and IgG to this bacteria using the TW-183 antigen. IgM and IgG reactivities to Chlamydia trachomatis serotypes C, D, E, and L2 and Chlamydia psittaci strain 6BC antigens were also tested. A sample was interpreted as positive only when evenly fluorescent elementary bodies were observed. Twenty-five subjects (4.9%) showed serological evidence of recent Chlamydia pneumoniae infection (IgM positive and (or) IgG seroconversion); 11 of them also showed serological evidence of recent infection with at least one other chlamydial species. Specificity was 50 and 63% for IgM and IgG detection, respectively. These results suggest that mixed or temporally related infections might occur, or, more likely, that some Chlamydia pneumoniae IgM or IgG reactivities might be due to heterotypic antibodies. Key words: TWAR serology, TWAR infections, TWAR cross-reactions.

scholarly journals Production of a Proteolytically Active Protein, Chlamydial Protease/Proteasome-Like Activity Factor, by Five Different Chlamydia Species

2005 ◽  
Vol 73 (3) ◽  
pp. 1868-1872 ◽  
Author(s):  
Feng Dong ◽  
Youmin Zhong ◽  
Bernard Arulanandam ◽  
Guangming Zhong
Keyword(s):  
Transcription Factors ◽  
Chlamydia Trachomatis ◽  
Chlamydia Pneumoniae ◽  
Chlamydia Psittaci ◽  
Active Protein ◽  
Chlamydia Muridarum ◽  
Chlamydial Species ◽  
Activity Factor ◽  
In Cells

ABSTRACT We have previously identified a chlamydial protein, chlamydial protease/proteasome-like activity factor (CPAF), for degrading host transcription factors in cells infected with the human chlamydial species Chlamydia trachomatis or Chlamydia pneumoniae. We now report that functional CPAF was also produced during infection with the species Chlamydia muridarum, Chlamydia psittaci, and Chlamydia caviae, which primarily infect nonhuman hosts.

The role of Chlamydia pneumoniae in severe acute tonsillitis

1994 ◽  
Vol 108 (2) ◽  
pp. 135-137 ◽  
Author(s):  
S. W. Hone ◽  
J. Moore ◽  
J. Fenton ◽  
P. K. Gormley ◽  
R. Hone
Keyword(s):  
Chlamydia Pneumoniae ◽  
Antibody Test ◽  
Control Group ◽  
Direct Immunofluorescence ◽  
Serological Evidence ◽  
Igg Antibody ◽  
Acute Tonsillitis ◽  
Recent Infection ◽  
College Hospital

AbstractChlamydia pneumoniae has been implicated as a cause of tonsillitis and pharyngitis, but the incidence has varied from one to 19 per cent in various studies. We investigated 51 patients admitted to University College Hospital, Galway, with severe tonsillitis. Throat swabs were examined for evidence of Chlamydia pneumoniae using a direct monoclonal antibody test. Blood was taken for serology from 45 patients. A further specimen was taken at six weeks. A control group of 32 blood bank sera was used. Mean hospital stay was three days (one to eight). Five patients (10 per cent) were monospot positive. Chlamydia pneumoniae was identified by direct immunofluorescence on a tonsillar swab from one patient who did not seroconvert. IgG antibody was identified in 13 cases (29 per cent) and in seven of the control group (22 per cent). No serological evidence of recent infection was found. Chlamydia pneumoniae was not found to be a cause of severe acute tonsillitis in our study group.

scholarly journals Touchdown Enzyme Time Release-PCR for Detection and Identification of Chlamydia trachomatis, C. pneumoniae, and C. psittaci Using the 16S and 16S-23S Spacer rRNA Genes

2000 ◽  
Vol 38 (3) ◽  
pp. 1085-1093 ◽  
Author(s):  
Guillermo Madico ◽  
Thomas C. Quinn ◽  
Jens Boman ◽  
Charlotte A. Gaydos
Keyword(s):  
Chlamydia Trachomatis ◽  
Dna Sequences ◽  
Chlamydia Pneumoniae ◽  
Chlamydia Psittaci ◽  
Rrna Genes ◽  
Clinical Samples ◽  
Species Differentiation ◽  
Analytical Sensitivity ◽  
Variable Regions ◽  
Primer Sets

Three touchdown enzyme time release (TETR)-PCR assays were used to amplify different DNA sequences in the variable regions of the 16S and 16S-23S spacer rRNA genes specific for Chlamydia trachomatis, Chlamydia pneumoniae, andChlamydia psittaci as improved tests for sensitive diagnosis and rapid species differentiation. The TETR-PCR protocol used 60 cycles of amplification, which provided improved analytical sensitivity (0.004 to 0.063 inclusion-forming unit ofChlamydia species per PCR). The sensitivity of TETR-PCR with primer set CTR 70-CTR 71 was 96.7%, and the specificity was 99.6%, compared to those of the AMPLICOR PCR for the detection ofC. trachomatis in vaginal swab samples. TETR-PCR forC. pneumoniae with primer set CPN 90-CPN 91 was 90% sensitive and 93.3% specific compared with a nested PCR with primer set CP1/2-CPC/D for clinical respiratory samples. TETR-PCR for C. psittaci with primer set CPS 100-CPS 101 showed substantial agreement with cell culturing (κ, 0.78) for animal tissue samples. Primer sets were then combined into a single multiplex TETR-PCR test. The respective 315-, 195-, and 111-bp DNA target products were precisely amplified when DNA from each of the respectiveChlamydia species or combinations of them was used. Multiplex chlamydia TETR-PCR correctly identified one strain of each of the 15 serovars of C. trachomatis, 22 isolates of C. pneumoniae, and 20 isolates of C. psittaci. The primer sets were specific for each species. No target products were amplified when DNA from C. pecorum or a variety of other microorganisms was tested for specificity. TETR-PCR with primers selected for specific sequences in the 16S and 16S-23S spacer rRNA genes is a valuable test that could be used either with individual primers or in a multiplex assay for the identification and differentiation of Chlamydia species from culture isolates or for the detection of chlamydiae in clinical samples.

scholarly journals Human Mannose-Binding Protein Inhibits Infection of HeLa Cells by Chlamydia trachomatis

1998 ◽  
Vol 66 (4) ◽  
pp. 1607-1612 ◽  
Author(s):  
Albertina F. Swanson ◽  
R. Alan B. Ezekowitz ◽  
Amy Lee ◽  
Cho-chou Kuo
Keyword(s):  
Chlamydia Trachomatis ◽  
Hela Cells ◽  
Chlamydial Infection ◽  
Chlamydia Pneumoniae ◽  
Binding Protein ◽  
Chlamydia Psittaci ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mannose Binding Protein ◽  
Mannose Binding ◽  
High Mannose

ABSTRACT The role that collectin (mannose-binding protein) may play in the host’s defense against chlamydial infection was investigated. Recombinant human mannose-binding protein was used in the inhibition of cell culture infection by Chlamydia trachomatis(C/TW-3/OT, E/UW-5/Cx, and L2/434/Bu), Chlamydia pneumoniae (AR-39), and Chlamydia psittaci (6BC). Mannose-binding protein (MBP) inhibited infection of all chlamydial strains by at least 50% at 0.098 μg/ml for TW-3 and UW-5, and at 6.25 μg/ml for 434, AR-39, and 6BC. The ability of MBP to inhibit infection with strain L2 was not affected by supplementation with complement or addition of an L2-specific neutralizing monoclonal antibody. Enzyme-linked immunosorbent assay and dot blot analyses showed MBP bound to the surface of the organism to exert inhibition, which appeared to block the attachment of radiolabeled organisms to HeLa cells. Immunoblotting and affinity chromatography indicated that MBP binds to the 40-kDa glycoprotein (the major outer membrane protein) on the outer surface of the chlamydial elementary body. Hapten inhibition assays with monosaccharides and defined oligosaccharides showed that the inhibitory effects of MBP were abrogated by mannose or high-mannose type oligomannose-oligosaccharide. The latter carbohydrate is the ligand of the 40-kDa glycoprotein ofC. trachomatis L2, which is known to mediate attachment, suggesting that the MBP binds to high mannose moieties on the surface of chlamydial organisms. These results suggest that MBP plays a role in first-line host defense against chlamydial infection in humans.

Chlamydia

2011 ◽  
pp. 190-191
Author(s):  
Dr Mark Harrison
Keyword(s):  
Chlamydia Trachomatis ◽  
Incubation Period ◽  
Chlamydia Pneumoniae ◽  
Chlamydia Psittaci ◽  
Genitourinary Tract ◽  
Respiratory Pathogen ◽  
Atypical Pneumonia ◽  
Intracellular Parasite ◽  
Obligate Intracellular ◽  
Obligate Intracellular Parasite

10.1 Chlamydia trachomatis, 191 • An obligate intracellular parasite • 3 species: ▪ Chlamydia trachomatis causes diseases of genitourinary tract and eye ▪ Chlamydia psittaci is a respiratory pathogen, transmitted by contact with birds, causes psittacosis ▪ Chlamydia pneumoniae causes atypical pneumonia • Incubation period 1-3 weeks...

scholarly journals Chlamydiae from Down Under: The Curious Cases of Chlamydial Infections in Australia

2019 ◽  
Vol 7 (12) ◽  
pp. 602
Author(s):  
Martina Jelocnik
Keyword(s):  
Chlamydia Pneumoniae ◽  
Research Field ◽  
Chlamydia Psittaci ◽  
Indigenous Populations ◽  
Future Directions ◽  
Sexually Transmitted ◽  
Chlamydial Species ◽  
Chlamydia Pecorum ◽  
Animal Pathogens ◽  
Human Infections

In Australia, the most researched and perhaps the most successful chlamydial species are the human pathogen Chlamydia trachomatis, animal pathogens Chlamydia pecorum and Chlamydia psittaci. C. trachomatis remains the leading cause of sexually transmitted infections in Australians and trachoma in Australian Indigenous populations. C. pecorum is globally recognised as the infamous koala and widespread livestock pathogen, whilst the avian C. psittaci is emerging as a horse pathogen posing zoonotic risks to humans. Certainly not innocuous, the human infections with Chlamydia pneumoniae seem to be less prevalent that other human chlamydial pathogens (namely C. trachomatis). Interestingly, the complete host range for C. pecorum and C. psittaci remains unknown, and infections by other chlamydial organisms in Australian domesticated and wildlife animals are understudied. Considering that chlamydial organisms can be encountered by either host at the human/animal interface, I review the most recent findings of chlamydial organisms infecting Australians, domesticated animals and native wildlife. Furthermore, I also provide commentary from leading Australian Chlamydia experts on challenges and future directions in the Chlamydia research field.

scholarly journals Prevalence of Chlamydia trachomatis, Chlamydia psittaci and Chlamydia pneumoniae antibodies in blood donors and attendees of STD clinics

1996 ◽  
Vol 1 (4) ◽  
pp. 253-260 ◽  
Author(s):  
Kristina Bergström ◽  
Marius Domeika ◽  
Daiva Vaitkiene ◽  
Kenneth Persson ◽  
Per-Anders Mårdh
Keyword(s):  
Chlamydia Trachomatis ◽  
Blood Donors ◽  
Chlamydia Pneumoniae ◽  
Chlamydia Psittaci
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