The use of bioluminescence as a reporter to study the adherence of the plant growth promoting rhizopseudomonads 7NSK2 and ANP15 to canola roots

1993 ◽  
Vol 39 (3) ◽  
pp. 329-334 ◽  
Author(s):  
J. Boelens ◽  
D. Zoutman ◽  
J. Campbell ◽  
W. Verstraete ◽  
W. Paranchych
Keyword(s):  
Plant Growth ◽  
Freundlich Isotherm ◽  
Root Surface ◽  
Broad Host Range ◽  
Bacterial Bioluminescence ◽  
Range Vector ◽  
Plant Growth Promoting ◽  
Specific Receptors ◽  
Growth Promoting ◽  
Kinetics Of

The adherence of the plant growth promoting rhizopseudomonads Pseudomonas aeruginosa 7NSK2 and Pseudomonas fluorescens ANP15 to canola roots (Brassica campestris L. c.v. Tobin) was examined by means of a bacterial bioluminescence system. The bioluminescence broad host range vector pDLUX-I was constructed from pLAFR-I and the lux A–E genes of Vibrio fischerii. This vector was conjugally transferred into the plant growth promoting rhizopseudomonads 7NSK2 and ANP15. The transformed strains were constitutively bioluminescent at an optimal temperature of 21 °C. The measured bioluminescence was directly proportional to the density of the bacteria in suspension and was the same for both planktonic and sessile bacteria adhering to the root surface. The adherence of the plant growth promoting rhizopseudomonads was proportional to the density of the bacterial inoculum, approached saturation at 60 min, and was reversible. The kinetics of the microbial adhesion was described by a Freundlich isotherm suggesting that the adherence of the bacteria to the canola root surface does not involve specific receptors. We conclude that the pDLUX-I vector is an easy and accurate way to study the kinetics of microbial adherence to the rhizoplane.Key words: rhizopseudomonads, bioluminescence, adhesion, plant growth promotion.not available

Adsorption kinetics of Pb and Cd by two plant growth promoting rhizobacteria

2009 ◽  
Vol 100 (20) ◽  
pp. 4559-4563 ◽  
Author(s):  
S.C. Wu ◽  
X.L. Peng ◽  
K.C. Cheung ◽  
S.L. Liu ◽  
M.H. Wong
Keyword(s):  
Plant Growth ◽  
Adsorption Kinetics ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Kinetics Of

scholarly journals Paenibacillus polymyxa Invades Plant Roots and Forms Biofilms

2005 ◽  
Vol 71 (11) ◽  
pp. 7292-7300 ◽  
Author(s):  
Salme Timmusk ◽  
Nina Grantcharova ◽  
E. Gerhart H. Wagner
Keyword(s):  
Plant Growth ◽  
Fluorescent Protein ◽  
Paenibacillus Polymyxa ◽  
Plant Roots ◽  
Root Tip ◽  
Broad Host Range ◽  
Plant Growth Promoting Bacteria ◽  
Soil System ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Paenibacillus polymyxa is a plant growth-promoting rhizobacterium with a broad host range, but so far the use of this organism as a biocontrol agent has not been very efficient. In previous work we showed that this bacterium protects Arabidopsis thaliana against pathogens and abiotic stress (S. Timmusk and E. G. H. Wagner, Mol. Plant-Microbe Interact. 12:951-959, 1999; S. Timmusk, P. van West, N. A. R. Gow, and E. G. H. Wagner, p. 1-28, in Mechanism of action of the plant growth promoting bacterium Paenibacillus polymyxa, 2003). Here, we studied colonization of plant roots by a natural isolate of P. polymyxa which had been tagged with a plasmid-borne gfp gene. Fluorescence microscopy and electron scanning microscopy indicated that the bacteria colonized predominantly the root tip, where they formed biofilms. Accumulation of bacteria was observed in the intercellular spaces outside the vascular cylinder. Systemic spreading did not occur, as indicated by the absence of bacteria in aerial tissues. Studies were performed in both a gnotobiotic system and a soil system. The fact that similar observations were made in both systems suggests that colonization by this bacterium can be studied in a more defined system. Problems associated with green fluorescent protein tagging of natural isolates and deleterious effects of the plant growth-promoting bacteria are discussed.

scholarly journals Insights from the Complete Genome Sequence of Bacillus circulans GN03, a Plant Growth-Promoting Bacterium Isolated from Pak Choi Cabbage (Brassica chinensis L.) Root Surface

2020 ◽  
Vol 9 (34) ◽  
Author(s):  
Lijun Qin ◽  
Xingyong Yang ◽  
Hong Shen
Keyword(s):  
Plant Growth ◽  
Genome Sequence ◽  
Gc Content ◽  
Root Surface ◽  
Bacillus Circulans ◽  
Whole Genome Sequence ◽  
Circular Chromosome ◽  
Pak Choi ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT A plant growth-promoting rhizobacterium, Bacillus circulans GN03, was isolated from the root surface of pak choi cabbage. Here, we report the whole-genome sequence of the GN03 strain, which includes a circular chromosome (5,217,129 bp; GC content, 35.64%) and a plasmid (181,705 bp; GC content, 31.62%).

Loblolly pine seedling growth after inoculation with plant growth-promoting rhizobacteria and ozone exposure

2004 ◽  
Vol 34 (7) ◽  
pp. 1410-1416 ◽  
Author(s):  
B L Estes ◽  
S A Enebak ◽  
A H Chappelka
Keyword(s):  
Plant Growth ◽  
Loblolly Pine ◽  
Plant Growth Promoting Rhizobacteria ◽  
Field Trials ◽  
Root Surface ◽  
Ozone Exposure ◽  
Root Surface Area ◽  
Foliar Injury ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting rhizobacteria promote plant growth and induce biocontrol, but are affected by soil type, water stress, microbial competition, and environmental conditions. One unexplored factor is the interaction of rhizobacteria-inoculated plants exposed to ozone. Loblolly pine (Pinus taeda L.) seeds were inoculated with either Bacillus subtilis (Ehrenberg) Cohn or Paenibacillus macerans (Schardinger) Ash. In field trials, 4-week-old seedlings were exposed for 12 weeks to carbon-filtered (CF ≈ 12 ppb), 1× (≈46 ppb), or 2× (≈97 ppb) ozone for 12 h·d–1 in open-top chambers (OTC) in 1998 and 1999. In three greenhouse trials, 5-week-old seedlings were exposed to ozone at 0× (≈8 ppb), 1× (≈105 ppb), 2× (≈199 ppb), and 3× (≈298 ppb) for 4 h·d–1, 5 d·week–1 for 8 weeks in continuously stirred tank reactors (CSTR). In both the CSTRs and the OTCs, ozone-exposed seedlings exhibited 20%–50% less biomass and more foliar injury as compared with nonexposed seedlings. In CSTRs, at the 3× exposure, B. subtilis-inoculated seedlings had 12% less foliar injury than noninoculated seedlings. Foliar injury was 65% less for B. subtilis-treated seedlings in 1998, and root surface area, total root length, and root diameter was 25%–35% greater when seedlings were exposed to 2× ozone in the OTCs. This is the first report of rhizobacteria protecting seedlings against the negative effects of ozone exposure.

Biological consequences of plasmid transformation of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2

1991 ◽  
Vol 37 (10) ◽  
pp. 796-799 ◽  
Author(s):  
Yuwen Hong ◽  
J. J. Pasternak ◽  
Bernard R. Glick
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Pseudomonas Putida ◽  
Root Elongation ◽  
Plant Growth Promoting Rhizobacteria ◽  
Siderophore Production ◽  
Broad Host Range ◽  
Metabolic Load ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pseudomonas putida GR12-2, a plant growth promoting rhizobacterium, was transformed with the broad host range plasmid pGSS15. The presence of the plasmid caused (i) a decrease in cell generation times, (ii) an altered pattern of cell proteins, (iii) an inhibition of the enhancement of canola root elongation, (iv) impairment of nitrogen fixation, and (v) a decrease in siderophore production. Strains that were cured of pGSS15, on the other hand, re-established growth rates, levels of siderophore production, and canola root elongation capabilities equivalent to nontransformed P. putida GR12-2. Thus, the transforming plasmid imposes a metabolic load on the recipient bacteria that impacts on a number of different energy-dependent processes. Key words: plant growth promoting rhizobacteria, nitrogen fixation, Pseudomonas, transformation, metabolic load.

scholarly journals A review on plant growth promoting rhizobacteria acting as bioinoculants and their biological approach towards the production of sustainable agriculture

2015 ◽  
Vol 7 (1) ◽  
pp. 540-556 ◽  
Author(s):  
Vibha Nehra ◽  
Madhu Choudhary
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Root Surface ◽  
Current Status ◽  
Plant Growth Promoting ◽  
Colonization Ability ◽  
Growth Promoting ◽  
Sustainable Agricultural Systems ◽  
Commercial Inoculants ◽  
Action Formulation

Plant growth promoting rhizobacteria are the soil bacteria inhabiting around/on the root surface and are directly or indirectly involved in promoting plant growth and development via production and secretion of various regulatory chemicals in the vicinity of rhizosphere. There has been much research interest in PGPB and there is now an increasing number of PGPB being commercialized for various crops. Today a lot of efforts have been made for searching and investigating the PGPB and their mode of action, so that they can be exploited commercially as biofertilizers. Because of the various challenges faced in screening, formulation, and application, PGPB have yet to fulfill their promise and potential as commercial inoculants. Recent progress in our understanding of their diversity, colonization ability, mechanisms of action, formulation, and application should facilitate their development as reliablecomponents in the management of sustainable agricultural systems. Several reviews have discussed specific aspects of PGPB as bioinoculants. We have tried to critically evaluate the current status of bacterial inoculants for contemporary agriculture in developed and developing countries. This review focuses on some important information regarding the biofertilizing potential of some important group of microbes, their formulations, their application for the development of sustainable technology, scope of improvement by genetic engineering, steps to be undertaken for their commercialization and their future prospects.

Overcoming the metabolic load associated with the presence of plasmid DNA in the plant growth promoting rhizobacteriumPseudomonas putidaGR12-2

1995 ◽  
Vol 41 (7) ◽  
pp. 624-628 ◽  
Author(s):  
Yuwen Hong ◽  
J. J. Pasternak ◽  
Bernard R. Glick
Keyword(s):  
Plant Growth ◽  
Plasmid Vector ◽  
Plant Growth Promoting Rhizobacteria ◽  
Broad Host Range ◽  
Metabolic Load ◽  
Broad Host Range Plasmid ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Lactamase Gene ◽  
Derivatives Of

When the broad host range plasmid vector pGSS15 was used to genetically transform the plant growth promoting rhizobacterium Pseudomonas putida GR12-2, the transformants were physiologically debilitated. It was postulated that the expression of the β-lactamase gene of pGSS15 caused a metabolic load resulting in the impaired functioning of the bacterium. To test this hypothesis, derivatives of pGSS15 that either lack the β-lactamase gene (pYH122) or in which a β-glucosidase gene was substituted for the β-lactamase gene (pYH124) were constructed and examined to see whether their presence also impaired the functioning of P. putida GR12-2. On the basis of growth rates, siderophore production, and the ability to stimulate canola root elongation in sterile growth pouches, neither of the newly constructed plasmids debilitated P. putida GR12-2. In addition, P. putida GR12-2 transformed with pYH124 facilitated the proliferation of the bacterium in minimal medium containing cellobiose at low temperature. This latter trait may enable P. putida GR12-2 to persist in the soil in competition with other microorganisms.Key words: plant growth promoting rhizobacteria, PGPR, bacterial fertilizer, soil bacteria, metabolic load, β-glucosidase

Persistence in soil of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 and genetically manipulated derived strains

1995 ◽  
Vol 41 (6) ◽  
pp. 445-451 ◽  
Author(s):  
Weizhen Tang ◽  
J. J. Pasternak ◽  
Bernard R. Glick
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Soil Bacteria ◽  
Plant Growth Promoting Rhizobacteria ◽  
Broad Host Range ◽  
Wild Type ◽  
Soil Persistence ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Genetically Manipulated

Transformation of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 with broad-host-range vectors can affect the growth of the bacterium, its ability to promote root elongation of canola seedlings under gnotobiotic conditions, and its persistence in soil. Plasmid transformants, and a transposon-mutagenized derivative of P. putida GR12-2, fell into two classes with respect to these three attributes: strains that were clearly diminished in these capabilities and strains that behaved like the nontransformed wild type. These differences can be accounted for by the imposition of a metabolic load that is created by some types of genetic modification that results in a physiological impairment of the modified bacterium and decreases its ability to function as a plant growth promoting rhizobacterium.Key words: plant growth promoting rhizobacteria, PGPR, bacterial fertilizer, soil bacteria, soil persistence, microcosm.

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