Overcoming the metabolic load associated with the presence of plasmid DNA in the plant growth promoting rhizobacteriumPseudomonas putidaGR12-2

1995 ◽  
Vol 41 (7) ◽  
pp. 624-628 ◽  
Author(s):  
Yuwen Hong ◽  
J. J. Pasternak ◽  
Bernard R. Glick
Keyword(s):  
Plant Growth ◽  
Plasmid Vector ◽  
Plant Growth Promoting Rhizobacteria ◽  
Broad Host Range ◽  
Metabolic Load ◽  
Broad Host Range Plasmid ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Lactamase Gene ◽  
Derivatives Of

When the broad host range plasmid vector pGSS15 was used to genetically transform the plant growth promoting rhizobacterium Pseudomonas putida GR12-2, the transformants were physiologically debilitated. It was postulated that the expression of the β-lactamase gene of pGSS15 caused a metabolic load resulting in the impaired functioning of the bacterium. To test this hypothesis, derivatives of pGSS15 that either lack the β-lactamase gene (pYH122) or in which a β-glucosidase gene was substituted for the β-lactamase gene (pYH124) were constructed and examined to see whether their presence also impaired the functioning of P. putida GR12-2. On the basis of growth rates, siderophore production, and the ability to stimulate canola root elongation in sterile growth pouches, neither of the newly constructed plasmids debilitated P. putida GR12-2. In addition, P. putida GR12-2 transformed with pYH124 facilitated the proliferation of the bacterium in minimal medium containing cellobiose at low temperature. This latter trait may enable P. putida GR12-2 to persist in the soil in competition with other microorganisms.Key words: plant growth promoting rhizobacteria, PGPR, bacterial fertilizer, soil bacteria, metabolic load, β-glucosidase

Biological consequences of plasmid transformation of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2

1991 ◽  
Vol 37 (10) ◽  
pp. 796-799 ◽  
Author(s):  
Yuwen Hong ◽  
J. J. Pasternak ◽  
Bernard R. Glick
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Pseudomonas Putida ◽  
Root Elongation ◽  
Plant Growth Promoting Rhizobacteria ◽  
Siderophore Production ◽  
Broad Host Range ◽  
Metabolic Load ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pseudomonas putida GR12-2, a plant growth promoting rhizobacterium, was transformed with the broad host range plasmid pGSS15. The presence of the plasmid caused (i) a decrease in cell generation times, (ii) an altered pattern of cell proteins, (iii) an inhibition of the enhancement of canola root elongation, (iv) impairment of nitrogen fixation, and (v) a decrease in siderophore production. Strains that were cured of pGSS15, on the other hand, re-established growth rates, levels of siderophore production, and canola root elongation capabilities equivalent to nontransformed P. putida GR12-2. Thus, the transforming plasmid imposes a metabolic load on the recipient bacteria that impacts on a number of different energy-dependent processes. Key words: plant growth promoting rhizobacteria, nitrogen fixation, Pseudomonas, transformation, metabolic load.

Persistence in soil of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 and genetically manipulated derived strains

1995 ◽  
Vol 41 (6) ◽  
pp. 445-451 ◽  
Author(s):  
Weizhen Tang ◽  
J. J. Pasternak ◽  
Bernard R. Glick
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Soil Bacteria ◽  
Plant Growth Promoting Rhizobacteria ◽  
Broad Host Range ◽  
Wild Type ◽  
Soil Persistence ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Genetically Manipulated

Transformation of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 with broad-host-range vectors can affect the growth of the bacterium, its ability to promote root elongation of canola seedlings under gnotobiotic conditions, and its persistence in soil. Plasmid transformants, and a transposon-mutagenized derivative of P. putida GR12-2, fell into two classes with respect to these three attributes: strains that were clearly diminished in these capabilities and strains that behaved like the nontransformed wild type. These differences can be accounted for by the imposition of a metabolic load that is created by some types of genetic modification that results in a physiological impairment of the modified bacterium and decreases its ability to function as a plant growth promoting rhizobacterium.Key words: plant growth promoting rhizobacteria, PGPR, bacterial fertilizer, soil bacteria, soil persistence, microcosm.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

A Study on the Farmers’ Awareness and Acceptance of Biofertilizers in Kottayam District

GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 425-431
Author(s):  
Subin Thomas ◽  
Dr. M. Nandhini
Keyword(s):  
Organic Matter ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Nutrient Cycle ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Structured Questionnaire ◽  
Area Data

Biofertilizers are fertilizers containing microorganisms that promote plant growth by improving the supply of nutrients to the host plant. The supply of nutrients is improved naturally by nitrogen fixation and solubilizing phosphorus. The living microorganisms in biofertilizers help in building organic matter in the soil and restoring the natural nutrient cycle. Biofertilizers can be grouped into Nitrogen-fixing biofertilizers, Phosphorous-solubilizing biofertilizers, Phosphorous-mobilizing biofertilizers, Biofertilizers for micro nutrients and Plant growth promoting rhizobacteria. This study conducted in Kottayam district was intended to identify the awareness and acceptance of biofertilizers among the farmers of the area. Data have been collected from 120 farmers by direct interviews with structured questionnaire.

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