Méthodes de qualification des immobilisations chimiques d'une enzyme sur un support solide

2005 ◽  
Vol 83 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Anne Belzil ◽  
Carmen Parent
Keyword(s):  
Carbonic Anhydrase ◽  
Covalent Binding ◽  
Qualitative Evaluation ◽  
Solid Support ◽  
Catalytic Transformation ◽  
Enzyme Binding ◽  
Bicarbonate Ions ◽  
Catalyst Immobilization ◽  
Immobilization Enzyme

Carbonic anhydrase was immobilized on a polymer thermoplastic to promote gaseous CO2 hydration into bicarbonate ions. Catalyst immobilization was realized through a series of chemical reactions enabling enzyme covalent binding to polyamide support. Different initial enzyme concentrations of 0.25, 0.50, 0.75, 1, and 2 mg/ml were verified. Different techniques were developed to assess enzyme attachment. The amount of bound protein was determined using Bradford dosage of proteins remaining in solution following CA II incubation with solid support. ELISA has given a qualitative evaluation of the protein, enabling a follow up of enzyme binding robustness as a function of time. p-nitrophenyl acetate hydrolysis and CO2 hydration were assessed by spectrophotometry and electrometry, respectively. The percentage of active enzyme following immobilization was measured using an esterase linear model. Catalytic transformation rates for gaseous CO2 hydration were calculated for each type of immobilization.Key words: immobilization, enzyme, esterase, hydration.[Journal translation]

Who Am I? A Life Story Intervention for Persons With Intellectual Disability and Psychiatric Problems

2016 ◽  
Vol 54 (3) ◽  
pp. 173-186 ◽  
Author(s):  
Gerben J. Westerhof ◽  
Janny Beernink ◽  
Anneke Sools
Keyword(s):  
Intellectual Disability ◽  
Life Story ◽  
Psychiatric Symptoms ◽  
Qualitative Evaluation ◽  
Obsessive Compulsive ◽  
Personal Learning ◽  
Compulsive Disorder ◽  
Life Review Therapy ◽  
Psychiatric Problems

Abstract This article describes an innovative intervention based on narrative and life review therapy that is tailored to people with intellectual disability (ID) and psychiatric problems. The current study provides a first evaluation of the intervention. A symptom checklist (SCL-90) was used in a pre- and post-follow-up design, and a qualitative evaluation of the intervention was carried out with 25 participants. Results showed large changes in psychiatric symptoms, in particular on depression, anxiety, obsessive-compulsive disorder, and interpersonal sensitivity. Participants were mainly positive in their general explicit evaluations of the therapy as well as on personal learning points, intervention-specific, group-related, and therapist-related aspects. It is concluded that the intervention is promising for the treatment of people with ID and psychiatric complaints.

scholarly journals The sustainability of knowledge brokerage of the mental health improvement outcomes framework in Scotland: a follow-up analysis

2020 ◽  
Vol 16 (1) ◽  
pp. 177-195
Author(s):  
John Connolly ◽  
Garth Reid ◽  
Monja Knoll ◽  
Wendy Halliday ◽  
Shirley Windsor
Keyword(s):  
Mental Health ◽  
National Level ◽  
Qualitative Evaluation ◽  
Network Governance ◽  
Health Improvement ◽  
Health Strategy ◽  
Knowledge Brokerage ◽  
Challenges And Opportunities ◽  
Strategy Planning

This is a follow-up study to Reid et al (2017) which considered the barriers and facilitators of getting knowledge into policy when using a knowledge brokering approach. The previous study analysed the use of strategies to reduce barriers to the use of evidence in mental health strategy planning in Scotland using outcome frameworks. The main facilitators highlighted were the importance of local champions, cooperation within partnership networks, and national-level support. The barriers were local implementation cultures, local time pressures, perceived complexities of the framework, and timeliness of the framework. The present article details the results of a follow-up qualitative evaluation of the sustainability of the mental health improvement outcomes framework with local planners. There is a dearth of literature which focuses on the sustainability of outcome frameworks and the findings of this study suggest that the barriers highlighted by Reid et al (2017) remain acute issues. However, there are further aspects for learning for knowledge brokers themselves in terms of national and local relations and the wider challenges and opportunities relating to network governance and policy reform agendas.

scholarly journals Virtual screening of combinatorial library of novel benzenesulfonamides on mycobacterial carbonic anhydrase II

2016 ◽  
Vol 63 (2) ◽  
pp. 1-6
Author(s):  
F. Dikant ◽  
F. Gáplovský ◽  
V. Garaj
Keyword(s):  
Carbonic Anhydrase ◽  
Combinatorial Library ◽  
Selection Index ◽  
Carbonic Anhydrase Ii ◽  
Free Energies ◽  
Binding Properties ◽  
Enzyme Binding ◽  
Drug Candidates ◽  
Structure Activity ◽  
Selective Activity

AbstractCombinatorial library of novel benzenesulfonamides was docked (Schrodinger Glide) into mycobacterial carbonic anhydrase (mtCA II) and human (hCA II) isoforms with an aim to find drug candidates with selective activity on mtCA II. The predicted selectivity was calculated based on optimized MM-GBSA free energies for ligand enzyme interactions. Selectivity, LogP (o/w) and interaction energy were used to calculate the selection index which determined the subset of best scoring molecules selected for further evaluation. Structure-activity relationship was found for fragment subsets, showing us the possible way regarding how to influence lipophilicity without affecting ligand-enzyme binding properties.

Condylar changes in patients with idiopathic condylar resorption: retrospective 2-year follow-up CBCT-based case–control study

2020 ◽  
Vol 42 (6) ◽  
pp. 619-625
Author(s):  
Cristina R Exposto ◽  
Peter Stoustrup ◽  
Kasper D Kristensen ◽  
Michel Dalstra ◽  
Thomas K Pedersen
Keyword(s):  
Case Control Study ◽  
Qualitative Evaluation ◽  
Vertical Displacement ◽  
Case Control ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Control Group ◽  
Radiological Appearance ◽  
Control Study

Summary Objectives To compare condylar development and changes in condylar radiological appearance in patients with idiopathic condylar resorption (ICR) to a healthy, age- and gender matched, control group. Materials and methods This case-control study included 16 ICR patients [mean age: 15years, 9 months; standard deviation (SD): 4 years) and 16 controls (mean age: 16 years, 8 months; SD: 4 years, 7 months), with diagnostic (T0) and 2-year follow-up (T1) CBCT examinations. Condylar changes were evaluated through changes in condylar neck angle (CNA), and the transversal, vertical and anteroposterior displacement of five condylar points between T0 and T1. The magnitude and direction of condylar changes were evaluated using vector analyses. A qualitative radiological evaluation of the TMJ was performed based on healthy, erosive and repaired morphological appearance. Linear and angular measurements were assessed using ANOVA and a Tukey post-hoc test, and vectors were tested using an independent-sample 2-tailed t-test. Fisher’s exact test was used for the qualitative evaluation. Results At T0, ICR patients exhibited decreased condylar height, smaller condylar width and posteriorly inclined CNA compared with the control group (P < 0.05). During observation, condylar vertical growth was smaller in the ICR group than in the control group (P < 0.05). Vector analysis showed an upward direction of vertical displacement for all condylar points in the control group; the ICR group showed a downward direction (P < 0.003). The radiological appearance of 75% of the ICR condyles and 94% of the control condyles did not change during the 2-year follow-up period. Conclusions ICR condyles displayed reduced vertical development compared with control condyles. The radiological appearance remained unchanged for most joints. Observed changes in radiological appearance did not always follow a progressive model of degenerative joint disease.

scholarly journals A Prospective Study of Long-Term Regenerative Endodontics Outcomes of Necrotic Immature Permanent Teeth: An 8-Year Follow-Up

Healthcare ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1670
Author(s):  
Sawsan T. Abu Zeid ◽  
Ruaa A. Alamoudi ◽  
Osama S. Alothmani ◽  
Abeer A. Mokeem Saleh ◽  
Amna Y. Siddiqui
Keyword(s):  
Prospective Study ◽  
Mineral Trioxide Aggregate ◽  
Qualitative Evaluation ◽  
Permanent Teeth ◽  
Term Outcome ◽  
Long Term Outcome ◽  
Regenerative Endodontics ◽  
Canal Diameter

For the management of necrotic immature teeth, regenerative endodontics offers the advantage of further root lengthening, thickening of dentin wall, and apical closure. This prospective study aimed to evaluate the long-term outcome of regenerative endodontics in immature necrotic permanent teeth. A total of 23 immature roots were medicated by triple antibiotic paste. After 21 days, bleeding was induced by over-instrumentation, and then mineral trioxide aggregate and coronal restoration were applied. Patients were scheduled for clinical and radiographic follow-up for 8 years. The radiographic changes of root dimensions were assessed using the ImageJ Plugin and statistically analyzed by Kruskal–Wallis test at a 95% confidence level. For qualitative evaluation, images were overlapped and analyzed using Photoshop software. All teeth were asymptomatic one month after the treatment. All teeth (n = 18) with preoperative periapical radiolucency showed complete resolution within 6–9 months. Recall rate at two, three, and eight years was 69.6%, 56.5%, and 34.8%, respectively. Continuous root development with a significant increase in root length and thickening of dentin wall accompanied by a significant decrease in apical canal diameter was seen at the end of the observation period (p < 0.001). In conclusion, the long-term outcome of regenerative endodontics revealed successful clinical and radiographic results with appropriate case selection.

scholarly journals A qualitative evaluation of an mHealth application to support patients with psoriasis in relation to follow-up consultations (Preprint)

10.2196/28882 ◽  
2021 ◽  
Author(s):  
Bettina Trettin ◽  
Dorthe Boe Danbjørg ◽  
Flemming Andersen ◽  
Steven Feldman ◽  
Hanne Agerskov
Keyword(s):  
Qualitative Evaluation

scholarly journals Frenulectomy with Diode Laser Technology in Paeditric Patients: Quantitative and Qualitative Evaluations. Randomized Double- Blind Clinical Trial

2020 ◽  
Vol 10 (12) ◽  
pp. 4114 ◽  
Author(s):  
Gian Luca Sfasciotti ◽  
Francesca Zara ◽  
Miriam Fioravanti ◽  
Mariana Guaragna ◽  
Gaspare Palaia ◽  
...  
Keyword(s):  
Diode Laser ◽  
Assessment Tool ◽  
Mouth Opening ◽  
Qualitative Evaluation ◽  
Control Group ◽  
Double Blind ◽  
Laser Technology ◽  
Qualitative And Quantitative ◽  
Double Blind Clinical Trial

Background: Ankyloglossia is an anatomical alteration of the frenulum length, volume, compactness, and insertion. It can lead to alterations in the ability to swallow and chewing, and dysfunctional coordination amongst cranio-facial muscles; these alterations are often responsible for respiratory alterations, skeletal malocclusions and dyslalia or the delay of speech. The aim of this study is to estimate the efficacy of lingual frenulectomy with diode-laser technology through a qualitative and quantitative evaluation. Methods: One hundred and twenty-five pediatric patients were recruited: 100 with a lingual pathological frenulum were randomly divided into four operating groups; the other 25 with a borderline pathological frenulum were recruited as a control group. Each patient was included in a follow-up program (T0-T1-T2-T3-T4) for a quantitative and qualitative evaluation: the first through an accurate measurement of Kotlow’s free tongue measurement (≥16), Mouth Opening with Tongue Tip to incisive papilla (MOTTIP), Maximal Intercisal Mouth Opening (MIO) and Protrusion; and the second using the Assessment Tool for Lingual Frenulum Function (ATLFF), Bristol Tongue Assessment Tool (BTAT), and grade of tongue functionality. Results: The increase of the aforementioned quantitative parameters was circa 10 mm, and all patients reacquired full functionality of the tongue. Conclusions: Diode-laser technology is efficient and innovative in the treatment of pathological lingual frenulums.

scholarly journals The effectiveness of subtenon triamcinolone injection for the treatment of macular edema in children with retinitis pigmentosa

2021 ◽  
Vol 16 (1) ◽  
pp. 5-12
Author(s):  
Lyudmila A. Katargina ◽  
Ekaterina V. Denisova ◽  
Maria A. Khrabrova ◽  
Natalia A. Osipova
Keyword(s):  
Visual Acuity ◽  
Carbonic Anhydrase ◽  
Retinitis Pigmentosa ◽  
Macular Edema ◽  
Repeated Injection ◽  
Carbonic Anhydrase Inhibitors ◽  
Before And After ◽  
Subtenon Injection ◽  
Observation Period

Aim: This study aimed to assess the functional and anatomical results of subtenon triamcinolone injections in children with retinitis pigmentosa (PR) and cystic macular edema (CME), refractory to local carbonic anhydrase inhibitors. Material and methods: We examined 11 children (9 girls, 2 boys) aged 9 to 17 years who underwent subtenon injections of triamcinolone (22 eyes). The follow-up period ranged from 1.6 to 33.1 months (average 9.711.6 months), the number of triamcinalone injections ranged from 1 to 11, averaging 3. Before and after injection, best corrected visual acuity (BCVA), the thickness and structure of the retina in the macular zone (optical coherence tomography [OCT] was performed by using the NIDEK RS-3000, Japan or the Spectralis, Heidelberg Engineering, Germany), and intraocular pressure (IOP) were assessed. Results: The CME height at the end of the observation period decreased in 10 cases (45.5%), and the average central retinal thickness decreased from 70 to 594 m (on average, 219.1183.4 m). In 12 eyes (54.5%), the CME height at the end of the observation period did not change significantly. BCVA improved in two eyes (9.1%) and did not change in the other cases. Two children (18%) developed ophthalmic hypertension in both eyes after two injections of triamcinalone. In one child, IOP returned to normal on the background of hypothetical therapy. In another child, due to non-compensation of IOP at the maximum hypotensive mode, sinus trabeculectomy was performed in both eyes, and IOP normalization was achieved. Conclusion: Subtenon injection of triamcinolone in children with CME against a background of PR is in most cases is an effective and safe method of treatment and can be recommended if carbonic anhydrase inhibitors are ineffective at reducing/ resorbing edema and maintaining or improving visual function. Considering that the action of triamcinolone is short lived, and its repeated injection is required, and the resorption of CME and an increase in visual acuity are not always achievable, it is necessary to continue the search for more effective treatment methods.

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