SEROLOGICAL EFFECTS OF ETHIONINE IN THE RABBIT

1954 ◽  
Vol 32 (2) ◽  
pp. 126-130 ◽  
Author(s):  
Christine E. Rice ◽  
P. Boulanger ◽  
E. Annau
Keyword(s):  
Body Weight ◽  
Protein Synthesis ◽  
Serum Albumin ◽  
Nitrogen Content ◽  
Guinea Pigs ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Complement ◽  
Nonprotein Nitrogen ◽  
Normal Limits

In relation to body weight, rabbits were found to tolerate a much lower dosage of ethionine than guinea pigs. Serum complement titers fell, however, even in rabbits receiving small daily doses of ethionine, an indication of a basal disturbance in the processes of protein synthesis. Total serum protein values remained within normal limits but globulin tended to increase in relation to albumin, whereas in many of the ethionine-injected guinea pigs previously studied, serum albumin had increased in relation to globulin. In rabbits given 250 mgm. daily doses of ethionine, a definite increase in the nonprotein nitrogen content of the serum was recorded.

SEROLOGICAL EFFECTS OF ETHIONINE IN THE RABBIT

1954 ◽  
Vol 32 (1) ◽  
pp. 126-130
Author(s):  
Christine E. Rice ◽  
P. Boulanger ◽  
E. Annau
Keyword(s):  
Body Weight ◽  
Protein Synthesis ◽  
Serum Albumin ◽  
Nitrogen Content ◽  
Guinea Pigs ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Complement ◽  
Nonprotein Nitrogen ◽  
Normal Limits

In relation to body weight, rabbits were found to tolerate a much lower dosage of ethionine than guinea pigs. Serum complement titers fell, however, even in rabbits receiving small daily doses of ethionine, an indication of a basal disturbance in the processes of protein synthesis. Total serum protein values remained within normal limits but globulin tended to increase in relation to albumin, whereas in many of the ethionine-injected guinea pigs previously studied, serum albumin had increased in relation to globulin. In rabbits given 250 mgm. daily doses of ethionine, a definite increase in the nonprotein nitrogen content of the serum was recorded.

PARALLEL STUDIES OF COMPLEMENT AND COAGULATION IV. EFFECT OF CARBON TETRACHLORIDE

1951 ◽  
Vol 29 (2) ◽  
pp. 48-58
Author(s):  
Christine E. Rice ◽  
Paul Boulanger ◽  
P. J. G. Plummer
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Serum Protein ◽  
Guinea Pigs ◽  
Fatty Degeneration ◽  
Blood Groups ◽  
Total Serum Protein ◽  
Total Serum ◽  
Coagulation Time ◽  
Complement Activity

To determine whether liver injury would result in a parallel decline in the complement titer and coagulative properties of the blood, groups of guinea pigs were given series of injections of the liver poison, carbon tetrachloride. Marked fatty degeneration of the liver, a decline in total serum protein and albumin, a decrease in complement activity, and a prolongation of coagulation time was observed in the treated animals. A general relationship was noted between the albumin-globulin ratio and the complement titer of the serum and between the complement titer and the coagulation time of the plasma.

scholarly journals Evaluation of a short-term ingestion of zearalenone, fumonisin, and aflatoxin mixture incorporated, at low concentration, into the diet of weanling piglets and the effect of an anti-mycotoxin feed additive

2018 ◽  
Vol 39 (4) ◽  
pp. 1819
Author(s):  
Antonio Diego Brandão Melo ◽  
Angela Oliveira ◽  
Geovane Rosa De Oliveira ◽  
Chiara Domingues ◽  
Kelly Mazutti ◽  
...  
Keyword(s):  
Protective Efficacy ◽  
Feed Additive ◽  
Total Serum Protein ◽  
Total Serum ◽  
Short Term ◽  
Low Concentration ◽  
Normal Limits ◽  
Serum Biochemical ◽  
Weanling Piglets ◽  
Dose Levels

An experiment was conducted to evaluate the toxic effects of short-term ingestion of a zearalenone, fumonisin, and aflatoxin mixture, at low concentration, into the diet of weanling piglets and to assess the protective efficacy of an anti-mycotoxin feed additive. For 21 days, piglets were exposed to control or multi-mycotoxin treatment with or without the anti-mycotoxin additive. Growth performance was measured after 21d. Blood samples were taken to serum biochemical analysis and for quantify levels of circulating mononuclear immune cells. Effects on organs weights and histological changes, and expression levels of COX-2 and TNF-? in the liver and jejunum were evaluated. Overall, the multi-mycotoxin treatment had no effect on measured variables, and no adverse histopathological changes in organs were observed. The total serum protein concentration was higher in animals that received the multi-mycotoxin treatment; however, levels remained within normal limits for weanling piglets. In conclusion, the short-term multi-mycotoxin mixture, at the dose levels evaluated in this study, seems not affect the health of weanling piglets through the evaluated parameters. The absence of toxicity associated with the multi-mycotoxin treatment rendered it impossible to evaluate the efficacy of the anti-mycotoxin additive.

Increased serum albumin, γ globulin, immunoglobulin IgG, and IgG2 and IgG4 in autism

2002 ◽  
Vol 32 (8) ◽  
pp. 1457-1463 ◽  
Author(s):  
J. CROONENBERGHS ◽  
A. WAUTERS ◽  
K. DEVREESE ◽  
R. VERKERK ◽  
S. SCHARPE ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Serum Protein ◽  
Viral Infections ◽  
Autoimmune Disorder ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Total Serum Protein ◽  
Total Serum ◽  
Igg Subclass ◽  
Serum Concentrations

Background. Research on the biological pathophysiology of autism has found some evidence that immune alterations may play a role in the pathophysiology of that illness. As a consequence we expected to find that autism is accompanied by abnormalities in the pattern obtained in serum protein electrophoresis and in the serum immunoglobulin (Ig) and IgG subclass profile.Method. We examined whether subjects with autism showed changes in total serum protein (TSP) and the serum concentrations of albumin, α1 globulin, α2 globulin, β globulin and γ globulins, IgA, IgM and IgG and the IgG subclasses IgG1, IgG2, IgG3 and IgG4, compared with normal controls.Results. We found significantly increased concentrations of TSP in autistic subjects, which were attributable to increased serum concentrations of albumin and γ globulin. Serum IgG, IgG2 and IgG4 were also significantly raised. In autism there were significant and positive correlations between social problems and TSP and serum γ globulin and between withdrawal symptoms and TSP and serum albumin and IgG.Conclusions. The results suggest that autism is characterized by increased TSP, a unique pattern obtained in serum protein electrophoresis, i.e. increased serum albumin and IgG, and by a specific IgG subclass profile, i.e. increased serum IgG2 and IgG4. The increased serum concentrations of IgGs in autism may point towards an underlying autoimmune disorder and/or an enhanced susceptibility to infections resulting in chronic viral infections, whereas the IgG subclass skewing may reflect different cytokine-dependent influences on autoimmune B cells and their products.

Total nitrogen, nonprotein nitrogen, and protein content in largemouth bass (Micropterus salmoides) with reference to quantitative protein estimates

1972 ◽  
Vol 50 (12) ◽  
pp. 1607-1610 ◽  
Author(s):  
A. J. Niimi
Keyword(s):  
Body Weight ◽  
Nitrogen Content ◽  
Protein Content ◽  
Total Nitrogen ◽  
Largemouth Bass ◽  
Micropterus Salmoides ◽  
Total Nitrogen Content ◽  
Nonprotein Nitrogen ◽  
Feeding Level

Nonprotein nitrogen (NPN) content in largemouth bass accounted for 9% of the total nitrogen content. This level was influenced by feeding level and probably body weight. A consideration for NPN is essential for quantitative protein estimates.

scholarly journals PENGARUH PEMBERIAN SERBUK EKSTRAK KELOPAK BUNGA ROSELA (Hibiscus sabdariffa L.) TERHADAP SISTEM IMUN TIKUS SPRAGUE DAWLEY

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Mardiah Mardiah ◽  
Dwi Aryanti Nur’utami ◽  
Arti Hastuti
Keyword(s):  
Serum Albumin ◽  
Serum Protein ◽  
Total Serum Protein ◽  
Total Serum ◽  
Hibiscus Sabdariffa ◽  
Sprague Dawley

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian serbuk ekstrak kelopak bunga rosela terhadap sistem imun tikus Sprague Dawley. Parameter yang diamati adalah leukosit, differensial leukosit, organ limfoid (limpa dan hati), total serum protein dan serum albumin dari darah tikus Sprague Dawley. Hewan uji dibagi atas 4 kelompok yang masing-masing terdiri dari 5 ekor tikus. Kelompok pertama adalah kelompok normal, kelompok 2 diberikan stimuno forte dosis 1,35 mg/KgBB, kelompok 3 diberikan serbuk ekstrak rosela I dosis 40,5 mg/KgBB, dan kelompok 4 diberikan serbuk ekstrak rosela II dosis 81 mg/KgBB secara sonde selama 28 hari. Pada hari ke-0 dan hari ke-29 dilakukan pengambilan darah. Analisa data menggunakan perhitungan selisih, yang hasilnya dibandingkan dengan nilai normal tikus kondisi sehat. Berdasarkan hasil penelitian pemberin serbuk ekstrak rosela I dosis 40,5 mg/KgBB KgBB dapat mempertahankan fungsi sistem imun pada tikus Sprague Dawley dengan peningkatan nilai rata-rata jumlah neutrofil segmen sebesar 42,2%, total serum protein sebesar 10,99g/dl, bobot limpa relatif sebesar 0,22% dan bobot hati relatif sebesar 3,27% yang masih dalam batas normal hewan coba dalam kondisi sehat sehingga dapat memberikan efek protektif terhadap serangan antigen. Kata kunci :  Serbuk ekstrak,  rosela, sistem imun

Role of Infrared lamps in cold stress alleviation during winter in Murrah calves

2016 ◽  
Author(s):  
Showkat A. Bhat ◽  
Bharat Bhushan ◽  
Narendra Kumar ◽  
S. A. Lone ◽  
Pranay Bharti ◽  
...  
Keyword(s):  
Body Weight ◽  
Cold Stress ◽  
Average Daily Gain ◽  
The Body ◽  
Total Serum Protein ◽  
Total Serum ◽  
Stress Alleviation ◽  
Newborn Calves ◽  
Infrared Lamps

The main aim of this study was to determine the role of Infrared lamps in cold stress alleviation during winter in Murrah calves. Ten newborn calves were randomly divided into two groups (G1 and G2) of five each. The calves of G1 were provided with no additional protection; however calves of G2 were protected against cold weather by using Infrared lamps. The body weight (kg) of calves was recorded at weekly interval. The blood samples collected at fortnightly interval were analyzed for Packed Cell volume (PCV, %), hemoglobin (Hb, g/dl), Total Serum Protein (TSP, g/l), albumin (g/l), globulin (g/l) and albumin globulin (A:G) ratio and hormones viz., triiodothyronine (T3, ng/ml), thyroxine (T4, ng/ml) and cortisol (ng/ml). The total body weight gain and average daily gain (ADG) was significantly (P<0.01) higher in G2 as compared to G1. The PCV values were significantly (P<0.05) higher on day 15 and day 45 in G1 than G2. The albumin and A:G Ratio were significantly (P<0.05) higher on day 60 and 45, respectively in G1 than G2. The cortisol levels were higher in G1 than G2 and differences were highly significant (P<0.01) on 15th day and significant (P<0.05) on 45th day. Significantly (P<0.01) higher values of T3 and T4 were observed on 15th and 45th day in G1 as compared to G2. On the basis of the results, it may be concluded that Infrared lamps can be effectively used to protect newborn calves from adverse conditions of winter and to improve their body growth performance.

scholarly journals Effect of Aloe vera juice on the hepatotoxicity induced by isoniazid drug

2011 ◽  
Vol 3 (2) ◽  
pp. 238-241
Author(s):  
G . V. Zodape
Keyword(s):  
Body Weight ◽  
Serum Alkaline Phosphatase ◽  
Quantitative Estimation ◽  
Aloe Vera ◽  
Total Serum Protein ◽  
Total Serum ◽  
Average Weight ◽  
Central Vein ◽  
Mild Degree ◽  
Serum Aspartate Aminotransferase

Aloe vera leaves were gently pressed and the juice obtained was collected in a sterile container. The yield was calculated based on weight of the extract compared to the weight of the leaves. Twenty (20) Wistar strain rats (average weight 120-150 g) were divided into four (4) groups containing five (5) rats in each group. Each group was fed on a different diet like the control rats were fed on commercial rat pellet; experimental rats fed orally with Aloe vera juice 1.0 ml/kg body weight and isoniazid drug (LD) 50ml/kg body weight. Blood samples from each group were taken after 30th day through cardiac puncture for estimation of liver function test. The extent of liver damage was assessed by quantitative estimation of serum alanine aminotransferase (serum ALT), serum aspartate aminotransferase (serum AST), serum alkaline phosphatase (serum ALP), serum acid phosphatase (serum ACP), total serum protein (albumin and globulin) and serum bilirubin. Our investigations showed that the level of bilirubin was found to be higher in aloe vera juice and isoniazid drug administered groups. The level of serum ALT was found to be highest in rat fed with isoniazid drug, whereas the level of serum AST was found lowest in rats administered with isoniazid drug. The levels of serum ALP and serum ACP were found higher in concentration in rats administered with isoniazid drug. While the level of total proteins (albumin and globulin) was found to be low in group administered with isoniazid drug. Histopathological assessment of liver revealed that the animal exposed to isoniazid drug alone showed multifocal mild degree periportal mononuclear cell infiltration. Histological lesions ranged from hepatocellar disintegration and vacuolation in the peri-central vein area to marked proliferation of the rough endoplasmic reticulum.The remaining groups, however showed normal lobular pattern of liver.

Standards for Total Serum Protein Assays— A Collaborative Study

1975 ◽  
Vol 21 (8) ◽  
pp. 1159-1166 ◽  
Author(s):  
Basil T Doumas
Keyword(s):  
Human Serum Albumin ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Serum Protein ◽  
Bovine Serum ◽  
Total Serum Protein ◽  
Total Serum ◽  
Final Reaction ◽  
Protein Assays

Abstract We have studied the standardization of total serum protein assay with the biuret reaction. Standard solutions were prepared from lyophilized preparations of human serum albumin and bovine serum albumin, with corrections made for volatile material and ash contents. These solutions and a solution of crystalline albumin standard were analyzed with a new stable biuret reagent, to establish absorptivity values (values for the absorbance of a 1 g/liter final reaction mixture). The mean values obtained were 0.302, 0.292, and 0.290 for human serum albumin, bovine serum albumin, and the crystalline albumin, respectively. We believe that the established absorptivity value will improve the accuracy of serum protein determinations. We studied the linearity of the relation between color produced and protein concentration, with use of the solutions described above and a serum pool. The color adheres to Beer's law up to the highest concentration tested: 3 g/liter for HSA and BSA, and 2.8 g/liter for serum in the final reaction mixture. The new biuret reagent has been stable for one year at room temperature. We recommend the use of bovine serum albumin as a primary standard for serum protein assays. It is inexpensive, easily available, and exhibits the best linearity in the biuret reaction.

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