Mono-O-(methylsulfonylethy)-d-glucoses

1967 ◽  
Vol 45 (3) ◽  
pp. 255-260 ◽  
Author(s):  
A. L. Bullock ◽  
V. O. Cirino ◽  
S. P. Rowland
Keyword(s):  
Liquid Chromatography ◽  
Acid Hydrolysis ◽  
Gas Liquid Chromatography ◽  
Vinyl Sulfone ◽  
Methyl Vinyl ◽  
Trimethylsilyl Derivatives ◽  
Hydrolysis Of ◽  
Methyl Vinyl Sulfone ◽  
Glucose Derivatives

Mono-O-(methylsulfonylethyl)-d-glucoses needed for comparison with the cleavage products obtained by acid hydrolysis of methyl vinyl sulfone modified cotton celluloses have been prepared. Several new glucose derivatives were prepared as intermediates in the synthesis of the desired 2-O-, 3-O-, and 6-O-(methylsulfonylethyl)-d-glucoses. The reactions were monitored by gas–liquid chromatography; use was made of trimethylsilyl derivatives as necessary. The substituted glucoses were obtained as glassy solids.

scholarly journals INFLUENCE OF HYDROLYTIC TREATMENT ON THE CONTENT OF REDUCING SUBSTANCES IN NEUTRAL-SULPHITE ALKALI

2021 ◽  
pp. 309-317
Author(s):  
Leysan Azatovna Mingazova ◽  
Yelena Vyacheslavovna Kryakunova ◽  
Zosia Albertovna Kanarskaya ◽  
Альберт Владимирович Kanarskiy ◽  
Igor' Vadimovich Kruchina-Bogdanov ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Enzymatic Hydrolysis ◽  
Acid Hydrolysis ◽  
Dry Matter ◽  
Gas Liquid Chromatography ◽  
Birch Wood ◽  
Carbohydrate Composition ◽  
Enzyme Preparations ◽  
Reducing Substances ◽  
Hydrolysis Of

The aim of this work is to develop a technology for the preparation of neutral-sulfite liquors formed during the production of fibrous semi-finished products - cellulose from birch wood - for subsequent use as a nutrient medium for the cultivation of microorganisms. Acid hydrolysis was carried out at a temperature of 100 °С at a ratio of a 10% sulfuric acid solution to a liquor sample of 1 : 1. Enzymatic hydrolysis of neutral sulfite liquors was carried out with the enzyme preparations Accellerase XY and Accellerase XC at 50±2 °C and 60±2 °C. The end of hydrolysis was determined by the cessation of the increase in the content of reducing substances (RS) in the hydrolyzate. The original neutral sulphite lye contained 9.4% dry matter, 21.7 g/l of reducing substances, pH 5.3±0.2. It has been shown that as a result of enzymatic hydrolysis, the content of insoluble dry residue in the hydrolyzate decreases to 8.32% and 8.41%, respectively, and during acid hydrolysis – to 7.8%. The content of RS in neutral sulfite lye after acid hydrolysis increases by an average of 3 times, while after enzymatic hydrolysis - a maximum of 2 times. It was found by gas-liquid chromatography that pentoses predominate in the obtained hydrolysates. Microbiological processing of media with a similar carbohydrate composition is possible by a number of strains of microorganisms capable of assimilating pentoses, for example, yeast-like fungi of the Saccharomycetaceae family and bacteria of the Enterobacteriaceae family.

Acid Hydrolysis of Urinary Estriol

1967 ◽  
Vol 13 (3) ◽  
pp. 186-195 ◽  
Author(s):  
Adolf E Schindler ◽  
Vannee Ratanasopa ◽  
Walter L Herrmann
Keyword(s):  
Liquid Chromatography ◽  
Specific Gravity ◽  
Acid Hydrolysis ◽  
Patient Management ◽  
Gas Liquid Chromatography ◽  
Significant Extent ◽  
Adequate Control ◽  
Hydrolysis Of ◽  
Linear Decline

Abstract One hundred and twenty determinations of urinary estriol were carried out following acid hydrolysis and gas liquid chromatography. Through the use of a radioactive standard, it was shown that, with an increase in specific gravity (1.001 to 1.025), a steady linear decline of estriol recovery occurred (93% to 56%). The presence of glucose (1-5 gm./100 ml.) gradually diminished estriol recovery (78.2 ± 9 to 24.9 ± 11). Similar effects were caused by galactose and lactose. lnulin, fructose, and sucrose led to an extensive drop of extractable estriol (more than 95% at 5 gm./100 ml.). An even more marked fall in estriol recovery was found in the presence of Mandelamine methenamine, and formaldehyde. Dimethylamine, albumin, and Urofix did not influence the recovery of estriol to a significant extent. These findings emphasize the need for adequate control of recovery whenever patient management is conducted according to urinary-estriol titers.

MONOSACCHARIDE COMPOSITION OF SELECTED CANADIAN PEATS

1980 ◽  
Vol 60 (1) ◽  
pp. 1-7 ◽  
Author(s):  
H. MORITA ◽  
W. G. MONTGOMERY
Keyword(s):  
Liquid Chromatography ◽  
Ion Exchange ◽  
Acid Hydrolysis ◽  
Monosaccharide Composition ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Fibre Content ◽  
Gas Liquid Chromatography ◽  
Chemical Feature ◽  
Hydrolysis Of

The neutral monosaccharides released by the acid hydrolysis of five peat profiles were analyzed by ion exchange chromatography and gas-liquid chromatography in order to ascertain whether monosaccharide composition can be used to differentiate peats. Glucose, mannose and galactose were the predominant monosaccharides found. Changes observed with depth in the relative abundances of the monosaccharides were not always correlated with the degree of decomposition as measured by fibre content or pyrophosphate index. The arabinose to xylose ratio was a diagnostic chemical feature which reflected the degree of decomposition of the peats.

STRUCTURAL COMPONENTS IN METHYL VINYL SULFONE MODIFIED COTTON CELLULOSE

1966 ◽  
Vol 44 (9) ◽  
pp. 1051-1058 ◽  
Author(s):  
S. P. Rowland ◽  
V. O. Cirino ◽  
A. L. Bullock
Keyword(s):  
Vinyl Sulfone ◽  
Structural Components ◽  
Methyl Vinyl ◽  
Trimethylsilyl Ethers ◽  
Low Degree ◽  
Quantitative Analyses ◽  
Liquid Chromatographic ◽  
Hydrolysis Of ◽  
Methyl Vinyl Sulfone ◽  
The Michael Addition

Methylsulfonylethyl cellulose was prepared at a low degree of substitution (0.11), which is conventional for the chemical modification of cotton, by the Michael addition of methyl vinyl sulfone to cotton fabric. The distribution of substituents in the anhydroglucose units was determined by hydrolysis of the molecular chain to glucose units, selective concentration of the substituted glucose components, gas-liquid chromatographic analyses of the methylsulfonylethylglucoses, and comparison with authentic samples of 2-O-, 3-O-, and 6-O-methylsulfonylethylglucoses in the form of trimethylsilyl ethers. The validity of each of these steps for quantitative analyses has been examined. The ratio of the 2-O-, 3-O-, and 6-O-monomethylsulfonylethylglucoses was found to be 0.20:0.03:1.0. No evidence was found for the presence of di- or tri-substituted glucoses.

Mannitol estimation in biological fluids by gas-liquid chromatography of trimethylsilyl derivatives.

1980 ◽  
Vol 26 (3) ◽  
pp. 441-443 ◽  
Author(s):  
M F Laker ◽  
J N Mount
Keyword(s):  
Liquid Chromatography ◽  
Biological Fluids ◽  
Internal Standard ◽  
Peak Height ◽  
Gas Liquid Chromatography ◽  
Height Measurement ◽  
Standard Solution ◽  
Trimethylsilyl Derivatives

Abstract We describe a procedure for estimating mannitol concentrations in biological fluids. Samples are mixed with internal standard solution (alpha-methylglucose), deproteinized if necessary, desalted, and dried. Specimens are then derivatized by adding pyridine/bis(trimethylsilyl)acetamide/trimethylchlorosilane and heating at 60 degrees C for 30 min. Samples are chromatographed on a 275-cm column of 10% OV-17, operated at 190 degrees C, and quantitated by peak-height measurement. The technique is linear, accurate, precise, sensitive, and free from interference. It has been used to measure mannitol in plasma, urine, and bile.

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