Comparison of media used to evaluateRhizobium leguminosarumbivarviciaefor phosphate-solubilizing ability

2009 ◽  
Vol 55 (7) ◽  
pp. 910-915 ◽  
Author(s):  
Jia Xie ◽  
J. Diane Knight ◽  
Mary E. Leggett
Keyword(s):  
Rhizobium Leguminosarum ◽  
Liquid Media ◽  
Solid Media ◽  
National Botanical Research Institute ◽  
Botanical Research ◽  
Phosphate Solubilizing ◽  
Ultimate Objective ◽  
Single Medium ◽  
Phosphate Medium ◽  
Ph Concentration

Rhizobium leguminosarum is well known for its ability to fix nitrogen (N). In addition, its capacity to solubilize phosphate (Ph) has been receiving attention in recent years. Our ultimate objective was to select a R. leguminosarum bv. viciae isolate with superior Ph-solubilizing ability. The first step was to identify a culture medium that is sensitive and effective in identifying the ability of R. leguminosarum bv. viciae isolates to solubilize Ph. Thirty isolates were evaluated for Ph solubilization in broth and on solid formulations of three media: yeast mannitol extract (YEM), National Botanical Research Institute phosphate nutrient medium (MNBRI), and Pikovskaya phosphate medium (PVK). All media contained 5 g/L CaHPO4as the only phosphorus (P) source. All 30 isolates increased the Ph concentration in liquid cultures, but the amount of Ph released into solution by individual isolates varied from one medium to another. In contrast, only a subset of the 30 isolates solubilized Ph on the solid cultures. Furthermore, some of the isolates that were able to solubilize Ph were only able to do so on a single medium. Regression analysis revealed no relationship between the Ph concentration in the liquid media and the zones of clearing on the solid media (p > 0.05). Although the pH of all of the liquid media dropped after 12 days of growth of the isolates, a relationship between Ph concentration and pH existed only for the MNBRI medium (r2= 0.485, p < 0.001). Increasing the amount of N in the MNBRI medium from 0.1 g/L of (NH4)2SO4to 0.5 g/L of (NH4)2SO4did not affect the amount of Ph in solution, but it profoundly reduced the survival of the R. leguminosarum by approximately 50-fold. Consequently, the surviving bacteria were either more efficient at solubilizing Ph in the high N media or organic acids released from the lysis of the dead cells solubilized the CaHPO4source.

Expression of the Rhizobium leguminosarum biovar phaseoli melA Gene in Other Rhizobia Does Not Require the Presence of the nifA Gene

1997 ◽  
Vol 24 (2) ◽  
pp. 195 ◽  
Author(s):  
Teresa Cubo ◽  
Francisco Romero ◽  
Jose M. Vinardell ◽  
Jose E. Ruiz-Sainz
Keyword(s):  
Rhizobium Leguminosarum ◽  
Regulatory Gene ◽  
Culture Conditions ◽  
Pigment Production ◽  
Liquid Media ◽  
Melanin Production ◽  
Melanin Biosynthesis ◽  
Tyrosinase Gene ◽  
Solid Media ◽  
Rhizobium Strains

Many different Rhizobium strains produce melanin (Mel+) when grown on solid media supplemented with L-tyrosine. The composition of the media and the culture conditions are of great importance for pigment production. Previous reports showed that some Rhizobium leguminosarum biovar phaseoli strains that produce the pigment in complete solid media (TY) failed to produce the pigment in minimal media (SY) supplemented with L-tyrosine or in TY liquid media. In this paper we have investigated different R. fredii, R. meliloti, R. etli and R. leguminosarum bv. trifolii and phaseoli strains (all of them Mel+ in solid media) for their ability to produce the pigment in liquid media. All Rhizobium species tested, except Rhizobium etli, were Mel+ in liquid media and in all cases the pigment yielded maximum absorption peaks at 280 and 315 nm. Melanin production by other bacteria (such as Vibrio, Streptomyces or Azospirillum) is enhanced by the presence of amino acids other that tyrosine. In this paper we show that the addition of L-methionine, which is not a precursor of rhizobial melanins, stimulated pigment production by Rhizobium cultures supplemented with L-tyrosine. The role of melanin production by Rhizobium strains is unclear. One hypothesis is that the Rhizobium tyrosinase, a bifunctional copper-containing enzyme that is essential for melanin biosynthesis, could detoxify polyphenolic compounds which might accumulate in senescing nodules. We show here that R. etli and R. fredii bacteroids produced melanin, which supports the idea that bacteroids contain the enzyme tyrosinase. Previous reports showed that, in R. leguminosarum bv. phaseoli strain 8002, the expression of the tyrosinase gene (melA) is dependent on the presence of nifA, a regulatory gene that is located in the symbiotic plasmid. However, transfer of R. leguminosarum bv. phaseoli melA gene to pSym-cured derivatives of R. leguminosarum bv. trifolii and viciae, R. fredii and Rhizobium sp. (Hedysarum) produced Mel+ transconjugants. DNA-hybridisation experiments showed that the pSym-cured strains did not contain any copy of nifA. Therefore, in contrast to the results reported on R. leguminosarum bv. phaseoli strain 8002, the expression of the melA gene in other rhizobia is not nifA-dependent. Key words: Rhizobium, melanin.

Recovery of Humic-Reducing Bacteria from a Diversity of Environments

1998 ◽  
Vol 64 (4) ◽  
pp. 1504-1509 ◽  
Author(s):  
John D. Coates ◽  
Debra J. Ellis ◽  
Elizabeth L. Blunt-Harris ◽  
Catherine V. Gaw ◽  
Eric E. Roden ◽  
...  
Keyword(s):  
Humic Substance ◽  
Electron Acceptor ◽  
Microbial Reduction ◽  
Acetate Oxidation ◽  
Liquid Media ◽  
Sedimentary Environments ◽  
Sediment Types ◽  
Solid Media ◽  
The Family ◽  
Reducing Bacteria

ABSTRACT To evaluate which microorganisms might be responsible for microbial reduction of humic substances in sedimentary environments, humic-reducing bacteria were isolated from a variety of sediment types. These included lake sediments, pristine and contaminated wetland sediments, and marine sediments. In each of the sediment types, all of the humic reducers recovered with acetate as the electron donor and the humic substance analog, 2,6-anthraquinone disulfonate (AQDS), as the electron acceptor were members of the familyGeobacteraceae. This was true whether the AQDS-reducing bacteria were enriched prior to isolation on solid media or were recovered from the highest positive dilutions of sediments in liquid media. All of the isolates tested not only conserved energy to support growth from acetate oxidation coupled to AQDS reduction but also could oxidize acetate with highly purified soil humic acids as the sole electron acceptor. All of the isolates tested were also able to grow with Fe(III) serving as the sole electron acceptor. This is consistent with previous studies that have suggested that the capacity for Fe(III) reduction is a common feature of all members of theGeobacteraceae. These studies demonstrate that the potential for microbial humic substance reduction can be found in a wide variety of sediment types and suggest thatGeobacteraceae species might be important humic-reducing organisms in sediments.

Culture of Helicobacter pylori from domestic water samples – the impact of strain variation on growth on solid and in liquid media

2006 ◽  
Vol 54 (3) ◽  
pp. 147-152 ◽  
Author(s):  
R.J. Owen ◽  
S.A. Chisholm ◽  
G. Brick ◽  
J.V. Lee ◽  
S. Surman-Lee ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Culture Media ◽  
Tap Water ◽  
Clinical Isolates ◽  
Strain Variation ◽  
Liquid Media ◽  
Domestic Water ◽  
Source Of Infection ◽  
Solid Media ◽  
The Impact

Helicobacter pylori is an important global human pathogen and there is growing evidence from PCR assays that contaminated drinking water might be a possible source of infection in some circumstances. There are no validated protocols for direct isolation but various culture media have been developed for possible environmental sampling. Our aim here was to investigate how inter-strain variation might affect the interpretation of results with such media. Two laboratory adapted reference strains and four recent clinical isolates were tested on four solid media and in ten liquid media. Considerable variation was found between strains in their ability to recover on the different media after stress exposure (suspension in sterile tap water). Generally, clinical isolates were less robust than the laboratory-adapted strains and, overall, the former required longer recovery times. Our findings highlighted the importance of using a range of isolates for evaluations, as examination of laboratory-adapted strains alone did not provide an accurate representation of the utility of media that may be used to recover H. pylori from water.

Bougainvillea: A Long Journey from Rio to Lucknow

2016 ◽  
Vol 2 (1-2) ◽  
Author(s):  
S. C. Sharma
Keyword(s):  
Germplasm Collection ◽  
South American ◽  
Botanic Garden ◽  
Tropical Regions ◽  
Reference Centre ◽  
National Botanical Research Institute ◽  
History Of ◽  
Bougainvillea Spectabilis ◽  
Royal Botanic Gardens ◽  
Botanical Research

Bougainvillea, native of South American sub-tropics, was first collected by Commerson, a French Botanist, from Rio de Janeiro, Brazil, who named it after Louis Antoine de Bougainville, the French navigator with whom he went on voyage around the world during 1766=1769. From its native sub-tropical regions, bougainvilleas were introduced to temperate regions of European countries, where these were grown in the glasshouse. The history of domestication of bougainvillea is 250 years old and in this period there have been many landmark in the bougainvillea cultivation. In India, Bougainvillea spectabilis was first introduced in Kolkata in 1860 from Europe and the improvement work on bougainvillea started in early 20th century, with the introduction of a few cultivars by the Agri-Horticultural Societies, Kolkata and Chennai. But it achieved its popularity in 1920 with the introduction of the cultivar ‘Mrs. Butt’ in Kolkata from Royal Botanic Gardens, Kew, England. Out of 10 species, only B. glabra Choisy, B. spectabilis Willdenow, B. peruviana Humboldt and Bonpland and a natural hybrid B. x buttiana Holttum and Standley, are of horticultural importance. Today, a large number of Bougainvillea cultivars (500 cvs.) are available from three to multibracted ones in various colour and shades to variegated foliage as aresult of natural selection and breeding. 50% of the Bougainvillea cultivars have been evolved in our country. India has been recognized as the International Registration Authority for Bougainvilleas. The Botanic Garden of CSIR-National Botanical Research Institute, Lucknow has rich germplasm collection of Bougainvillea (200 species/cvs) and serves as the reference centre.

Effect of phosphorus on root colonization and growth promotion of maize by bioluminescent mutants of phosphate-solubilizing Rhizobium leguminosarum biovar phaseoli

1998 ◽  
Vol 30 (12) ◽  
pp. 1615-1618 ◽  
Author(s):  
Rock Chabot ◽  
Chantal J. Beauchamp ◽  
Joseph W. Kloepper ◽  
Hani Antoun
Keyword(s):  
Rhizobium Leguminosarum ◽  
Root Colonization ◽  
Growth Promotion ◽  
Phosphate Solubilizing

scholarly journals Trans-Kingdom Conjugation Within Solid Media from Escherichia coli to Saccharomyces cerevisiae

2019 ◽  
Author(s):  
Maximillian P.M. Soltysiak ◽  
Rebecca S. Meaney ◽  
Samir Hamadache ◽  
Preetam Janakirama ◽  
David R. Edgell ◽  
...  
Keyword(s):  
Donor Cell ◽  
Dna Delivery ◽  
Dna Transfer ◽  
Liquid Media ◽  
E Coli ◽  
Solid Media ◽  
Wide Range ◽  
Semi Solid ◽  
Plasmid Size ◽  
Synthesis Protocol

Conjugation is a bacterial mechanism for DNA transfer from a donor cell to a wide range of recipients, including both prokaryotic and eukaryotic cells. In contrast to conventional DNA delivery techniques, such as electroporation and chemical transformation, conjugation eliminates the need for DNA extraction, thereby preventing DNA damage during isolation. While most established conjugation protocols allow for DNA transfer in liquid media or on a solid surface, we developed a procedure for conjugation within solid media. Such a protocol may expand conjugation as a tool for DNA transfer to species that require semi-solid or solid media for growth. Conjugation within solid media could also provide a more stable microenvironment in which the conjugative pilus can establish and maintain contact with recipient cells for the successful delivery of plasmid DNA. Furthermore, transfer in solid media may enhance the ability to transfer plasmids and chromosomes greater than 100 kbp. Using our optimized method, plasmids of varying sizes were tested for transfer from E. coli to S. cerevisiae. We demonstrated that there was no substantial decrease in conjugation frequency as plasmid size increased&mdash;up to 138.5 kbp in length. Finally, we established an efficient PCR-based synthesis protocol to generate custom conjugative plasmids

scholarly journals Profiling the Production of Antimicrobial Secondary Metabolites by Xenorhabdus khoisanae J194 Under Different Culturing Conditions

2021 ◽  
Vol 9 ◽  
Author(s):  
Elzaan Booysen ◽  
Marina Rautenbach ◽  
Marietjie A. Stander ◽  
Leon M. T. Dicks
Keyword(s):  
Growth Conditions ◽  
Antifungal Compounds ◽  
Antimicrobial Compounds ◽  
Liquid Media ◽  
Whole Genome Analysis ◽  
Endosymbiotic Bacteria ◽  
Solid Media ◽  
Culturing Conditions ◽  
Antibacterial And Antifungal ◽  
Cells Cultured

Species from the genus Xenorhabdus, endosymbiotic bacteria of Steinernema nematodes, produce several antibacterial and antifungal compounds, some of which are anti-parasitic. In this study, we report on the effect growth conditions have on the production of antimicrobial compounds produced by Xenorhabdus khoisanae J194. The strain was cultured in aerated and non-aerated broth, respectively, and on solid media. Production of antimicrobial compounds was detected after 24 h of growth in liquid media, with highest levels recorded after 96 h. Highest antimicrobial activity was obtained from cells cultured on solid media. By using ultraperformance liquid chromatography linked to mass spectrometry and HPLC, a plethora of known Xenorhabdus compounds were identified. These compounds are the PAX lipopeptides (PAX 1′, PAX 3′, PAX 5, and PAX 7E), xenocoumacins and xenoamicins. Differences observed in the MS-MS fractionation patterns collected in this study, when compared to previous studies indicated that this strain produces novel xenoamicins. Three novel antimicrobial compounds, khoicin, xenopep and rhabdin, were identified and structurally characterized based on MS-MS fractionation patterns, amino acid analysis and whole genome analysis. The various compounds produced under the three different conditions indicates that the secondary metabolism of X. khoisanae J194 may be regulated by oxygen, water activity or both. Based on these findings X. khoisanae J194 produce a variety of antimicrobial compounds that may have application in disease control.

scholarly journals Study of Trichoderma Spp. Application on the Incidence of Moler Disease and Shallot's Growth and Yield

2021 ◽  
Vol 7 (2) ◽  
pp. 61-67
Author(s):  
Yana Mulyana ◽  
Mariana Mariana ◽  
Joko Purnomo
Keyword(s):  
Disease Incidence ◽  
Growth And Yield ◽  
Experimental Unit ◽  
Application Time ◽  
Liquid Media ◽  
Trichoderma Spp ◽  
Solid Media ◽  
Number Of Leaves ◽  
Fusarium Sp ◽  
Chemical Pesticides

Fusarium wilt disease/moler is a concern in onion cultivation. Many farmers apply chemical pesticides such as azoxystrobin and difenoconazole to control the diseases. Both of these chemical pesticides are currently exhibiting a decline in effectiveness, prompting farmers to increase the dosage and frequency of application. Recommendations for biological control, including Trichoderma spp.. This study aimed to determine the influence of application time of of Trichoderma spp. in various media types on the incidence of moler disease and shallot growth and yield. This study was conducted in Tabalong district, South Kalimantan, from February to June 2019 using a completely randomized design (CRD) factorial comprising two nested designs. The first factor was the type of media in which Trichoderma spp.. was applied, namely solid and liquid. The second factor was the application time of Trichoderma spp.. Level 1 was the application of Fusarium sp. seven days before planting and Trichoderma spp.. seven days after planting. Level 2 included the application of Trichoderma spp.. seven days before planting and Fusarium sp. simultaneously during planting. Level 3 involved the administration of Trichoderma spp.. concurrently with planting and Fusarium sp. seven days later. This investigation used one positive control (without treatment) and one negative control (shallots inoculated with Fusarium). Each experimental unit was consisted of ten plants and replicated three times. The parameters observed were the incubation period of Fusarium sp., disease incidence, number of leaves, plant height, number of cloves, and average clove weight. The study found that the application time of Trichoderma on various types of media affected the incidence of Fusarium disease, the number of leaves, and the number of shallot cloves.When compared to liquid media, the application of solid media resulted in a reduced incidence of Fusarium disease and a greater frequency of flares. The application time on solid media gave a low response to moles. As for liquid media, the lowest incidence of moler disease occurred at the seven days before planting.

Limiting Conditions of Temperature and pH for Growth of “Thermophilic” Campylobacters on Solid Media

1983 ◽  
Vol 46 (9) ◽  
pp. 767-768 ◽  
Author(s):  
C. O. GILL ◽  
LYNDA M. HARRIS
Keyword(s):  
Nalidixic Acid ◽  
Campylobacter Jejuni ◽  
Liquid Culture ◽  
Campylobacter Coli ◽  
Liquid Media ◽  
Ph Values ◽  
Solid Media ◽  
Temperature Ranges ◽  
Type Strains

Strains of Campylobacter previously used for studies with meat, included strains distinguishable as Campylobacter jejuni and nalidixic acid-resistant thermophilic campylobacters (NARTC). The pH and temperature minima for growth on agar plates were determined for 12 strains: six strains used in the meat studies (four C. jejuni, two NARTC), three type strains (C. jejuni, Campylobacter coli, NARTC) and three strains of C. jejuni whose pH and temperature ranges in liquid culture had been determined by other workers. Heavy, visible inocula of most strains grew at temperatures 2°C lower and pH values 0.2 or 0.3 unit lower than the minima observed with light inocula. For all strains of C. jejuni, values for temperature and pH minima from heavy inocula, 32°C and pH 5.1, were comparable with those reported for growth of three strains in liquid media. The pH minima for NARTC strains (pH 5.8) were higher than those for C. jejuni, but temperature minima were similar.

scholarly journals Isolation and screening of multifunctional rhizobacteria from the selected sites of Madhupur, Narshingdi and Mymensingh, Bangladesh

2015 ◽  
Vol 2 (1) ◽  
pp. 1-8
Author(s):  
Moonmoon Nahar Asha ◽  
Atiqur Rahman ◽  
Quazi Forhad Quadir ◽  
Md Shahinur Islam
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Plant Growth Promoting Rhizobacteria ◽  
N Fixation ◽  
National Botanical Research Institute ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Botanical Research ◽  
Plant Growth Promoting Traits

A laboratory experiment was performed to isolate some native rhizobacteria that could be used as bioinoculants for sustainable crop production. A total of 43 rhizobacteria were isolated from undisturbed plant rhizosphere soils of three different locations of Bangladesh and evaluated their plant growth promoting traits, both direct and indirect. The study has screened out isolates on the basis of their phosphorous solubilization and nitrogen (N) fixation. The phosphate solubilization assay in National Botanical Research Institute of Phosphate (NBRIP) medium revealed that 12 bacterial isolates were able to solubilize tricalcium phosphate and the rhizobacteria M25 showed best performance with a PSI of 3.33 at 5 day. Exactly 47% (20 isolates) of the isolated rhizobacteria were able to grow in N-free Winogradsky’s medium, which is an indication of potential N2-fixers. Among the 20 potential N-fixers, 15 were able to grow within 24 hours of incubation indicating that they are more efficient in Nfixation. The present study successfully isolated and characterized 43 rhizobacteria. Some of these isolated rhizobacteria have potential plant growth promoting traits and are potential plant growth promoting rhizobacteria (PGPR) candidate. Considering all plant growth promoting traits, the isolate F37 was the best followed by M6. However, further experiments are needed to determine the effectiveness of these isolates under in vitro and different field conditions to understand the nature of interaction with the plant and environment.Res. Agric., Livest. Fish.2(1): 1-8, April 2015

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