BLOOD TRANSAMINASE ACTIVITIES IN VITAMIN B6 DEFICIENCY: SPECIFICITY AND SENSITIVITY

1965 ◽  
Vol 43 (4) ◽  
pp. 579-589 ◽  
Author(s):  
M. C. Cheney ◽  
D. M. Curry ◽  
G. H. Beaton
Keyword(s):  
Vitamin B6 ◽  
B Vitamins ◽  
Xanthurenic Acid ◽  
Vitamin B ◽  
Transaminase Activity ◽  
Liver Transaminase ◽  
Dietary Protein Level ◽  
Specificity And Sensitivity ◽  
Vitamin B6 Deficiency

The lowering of blood glutamic–oxaloacetic (GOT) and glutamic–pyruvic (GPT) transaminase activities was found to be specific for vitamin B6 deprivation among several B vitamins tested and in the presence of a simultaneous restriction of eight B vitamins, cortisone administration, or variation of dietary protein level. It was found that changes in blood transaminase activity did not always parallel those seen in liver transaminase activity. In the determination of vitamin B6 nutritional status, blood GPT activity appeared to be more sensitive than GOT activity and would seem to be as sensitive an indicator as xanthurenic acid excretion after a tryptophan load.

BLOOD TRANSAMINASE ACTIVITIES IN VITAMIN B6 DEFICIENCY: EFFECT OF CONCURRENT THIAMINE AND RIBOFLAVIN DEFICIENCIES

1965 ◽  
Vol 43 (4) ◽  
pp. 591-599 ◽  
Author(s):  
M. C. Cheney ◽  
G. H. Beaton
Keyword(s):  
Body Weight ◽  
Nutritional Status ◽  
Factorial Design ◽  
Vitamin B6 ◽  
Vitamin B ◽  
Transaminase Activity ◽  
Liver Transaminase ◽  
Vitamin B6 Deficiency ◽  
Design Experiments ◽  
Concurrent Variation

Factorial design experiments in rats revealed that blood transaminase activity was sensitive to vitamin B6 dosage and was not affected by concurrent variation in riboflavin or thiamine administration.The blood transaminase activity was a better index of vitamin B6 nutritional status than was body weight response or liver transaminase activity. Similarly, erythrocyte transketolase activity was found to reflect thiamine dosage, and plasma riboflavin level to reflect riboflavin dosage, regardless of manipulation of pyridoxine dosage.

Determination of Vitamin B6 Deficiency in Rainbow Trout (Salmo gairdneri) by Liver Enzyme Assay and HPLC Analysis

1987 ◽  
Vol 44 (1) ◽  
pp. 219-222 ◽  
Author(s):  
Ronald W. Hardy ◽  
Edmundo Casillas ◽  
Toshiro Masumoto
Keyword(s):  
Rainbow Trout ◽  
Vitamin B6 ◽  
Enzyme Assay ◽  
Clinical Signs ◽  
Salmo Gairdneri ◽  
Vitamin B ◽  
Deficient Diet ◽  
Vitamin B6 Deficiency ◽  
Stimulation Of

Rainbow trout (Salmo gairdneri), initially averaging 125 g, were fed a complete or a pyridoxine-deficient diet for 14 wk. Vitamin B6 status was evaluated biweekly by direct measurement of liver pyridoxine and pyridoxal levels by HPLC and by determining pyridoxal-5′-phosphate-enhanced liver aspartate aminotransferase (ASAT) activity. By 14 wk, mortality had severely reduced the number of fish remaining in the pyridoxine-deficient group. At 14 wk, no significant differences in liver pyridoxine and pyridoxal levels were detected between the trout fed the complete or pyridoxine-deficient diet. Significant differences between dietary groups are found in ASAT activity in liver and percent stimulation of liver ASAT by the addition of pyridoxal-5′-phosphate after 8 wk. Clinical signs of vitamin B6 deficiency including anorexia, listlessness, frantic and erratic swimming, and ataxia were observed after 11 wk of feeding a pyridoxine-deficient diet. This study shows that vitamin B6 deficiency in rainbow trout can be readily determined weeks before signs of clinical deficiency are apparent by measuring pyridoxine-enhanced liver ASAT activity. However, liver levels of pyridoxine and pyridoxal are not sensitive indicators of vitamin B6 status.

Roth-like Spot in an Adult With Vitamin B6 Deficiency and Nonepileptic Seizures

2021 ◽  
pp. 247412642110287
Author(s):  
Diana Driscoll ◽  
David Callanan
Keyword(s):  
Differential Diagnosis ◽  
Adult Patient ◽  
Vitamin B6 ◽  
Vitamin B ◽  
Case Review ◽  
Single Patient ◽  
Retinal Lesion ◽  
History Of ◽  
Nonepileptic Seizures ◽  
Vitamin B6 Deficiency

Purpose: This work reports retinal findings in an adult patient with vitamin B6 deficiency. Methods: A case review of a single patient is presented. Results: A patient with a Roth-type retinal lesion and a history of nonepileptic seizures was found to have lymphocytic colitis. She was treated with pyridoxine, which resolved her seizures and the white-centered hemorrhage. Conclusions: Vitamin B6 deficiency should be considered in the differential diagnosis of patients presenting with white-centered hemorrhages and a history of nonepileptic seizures.

scholarly journals A Direct Assay for Pyridoxal 5′-Phosphate Using Pig Heart Apo-Aspartate Transaminase

1986 ◽  
Vol 23 (3) ◽  
pp. 317-324 ◽  
Author(s):  
Ratree Singkamani ◽  
D J Worthington ◽  
D I Thurnham ◽  
T P Whitehead
Keyword(s):  
Enzyme Activity ◽  
Vitamin B6 ◽  
Aspartate Transaminase ◽  
Enzymatic Method ◽  
Government Service ◽  
Sample Extract ◽  
Sample Extraction ◽  
Extraction Step ◽  
Vitamin B6 Deficiency

The development of a simple enzymatic method for the determination of blood and plasma pyridoxal 5′-phosphate (PLP) using pig heart apo-aspartate transaminase (apo-AST) is described. The technique requires three steps: (1) sample extraction using perchloric acid, (2) a binding step in which PLP in the sample extract is attached to the apo-AST and (3) the enzymatic assay of the reconstituted holo-AST. PLP extracts were analysed with and without a known concentration of added PLP to correct for variation in recovery between different specimens. Procedures are outlined for manual and automatic analysis of the PLP extracts. Using the KONE Clinical Analyser after the extraction step, it is possible to measure enzyme activity in 100 specimens (i.e. 400 tubes) in a 5 h period. Results are shown from 185 healthy women aged 20–45 years, in which plasma PLP concentrations ranged from 5 to 165 nmol/L, and 142 men and 56 women in Government service aged 17–64 years, whose plasma PLP ranged from 8 to 169 nmol/L. Values less than 20 nmol/L are believed to indicate vitamin B6 deficiency and the method is able to measure 5 nmol/L.

scholarly journals Tryptophan metabolism in vitamin B6-deficient mice

1990 ◽  
Vol 63 (1) ◽  
pp. 27-36 ◽  
Author(s):  
David A. Bender ◽  
Eliud N. M. Njagi ◽  
Paul S. Danielian
Keyword(s):  
Urinary Excretion ◽  
Intraperitoneal Injection ◽  
Vitamin B6 ◽  
Isolated Hepatocytes ◽  
Tryptophan Metabolism ◽  
Xanthurenic Acid ◽  
Vitamin B ◽  
Tryptophan Metabolites ◽  
Deficient Mice

Vitamin B6 deficiency was induced in mice by maintenance for 4 weeks on a vitamin B6-free diet. Tryptophan metabolism was assessed by determining the urinary excretion of tryptophan metabolites, the metabolism of [14C]tryptophan in vivo and the formation of tryptophan and niacin metabolites by isolated hepatocytes. The vitamin B6-deficient animals excreted more xanthurenic acid and 3-hydroxykynurenine, and less of the niacin metabolites N1-methyl nicotinamide and methyl-2-pyridone-4-carboxamide, than did control animals maintained on the same diet supplemented with 5 mg vitamin B6/kg. After intraperitoneal injection of [14C]tryptophan, vitamin B6-deficient mice showed lower liberation of14CO2 from [methylene-14C]tryptophan and [U-14C]tryptophan than did controls, indicating impairment of kynureninase (EC 3.7.1.3) activity. There was no difference between the two groups of animals in the metabolism of [ring-2-14C]tryptophan. Hepatocytes isolated from the vitamin B6-deficient animals formed more 3-hydroxykynurenine and xanthurenic acid than did cells from control animals, but also formed more NADP and free niacin.

scholarly journals The vitamin B6 status of pigs given a diet containing linseed meal

1977 ◽  
Vol 37 (3) ◽  
pp. 321-331 ◽  
Author(s):  
H. N. Bishara ◽  
H. F. Walker
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Vitamin B6 ◽  
Nitrogen Retention ◽  
Basal Diet ◽  
Xanthurenic Acid ◽  
Vitamin B ◽  
Linseed Meal ◽  
Load Tests

1. Pigs consuming a diet containing 300 g linseed meal/kg and a pyridoxine supplement showed greater growth, nitrogen retention, blood packed cell volume and haemoglobin than those receiving only the basal diet.2. Tryptophan-load tests on unsupplemented pigs revealed an increased excretion of kynurenine, Nα-acetylkynurenine and xanthurenic acid compared to those receiving additional pyridoxine.3. The results suggest that the unsupplemented pigs were marginally deficient in vitamin B6.4. When the same diet was fed to rats there was no evidence of vitamin B6 deficiency.

BLOOD TRANSAMINASE ACTIVITIES IN VITAMIN B6 DEFICIENCY: EFFECT OF DEPLETION AND REPLETION ON THE ERYTHROCYTE ENZYMES

1967 ◽  
Vol 45 (2) ◽  
pp. 343-351 ◽  
Author(s):  
M. C. Cheney ◽  
Z. I. Sabry ◽  
G. H. Beaton
Keyword(s):  
Nutritional Status ◽  
Vitamin B6 ◽  
The State ◽  
Rapid Response ◽  
Vitamin B ◽  
Red Cell ◽  
Cell Turnover ◽  
Erythrocyte Enzymes ◽  
Liver Transaminases ◽  
Vitamin B6 Deficiency

To assess the usefulness of the erythrocyte transaminases, glutamic–oxaloacetic and glutamic–pyruvic, in the evaluation of vitamin B6 nutritional status, the response of these enzymes to repletion following 1, 4, and 10 weeks of vitamin B6 depletion was compared with that of the plasma and liver transaminases in the rat. The responses of the erythrocyte enzymes reflected the state of vitamin B6 nutrition most accurately and, unlike the plasma and liver transaminases, were not affected by other factors. Repletion produced a rapid response in the erythrocyte enzymes; however, restoration of control levels appeared to depend on red cell turnover, particularly after prolonged depletion periods. This finding suggests that the apoenzyme, as well as the coenzyme, is depleted during vitamin B6 deficiency.

THE STATE OF VITAMIN B6 DEFICIENCY AS MEASURED BY URINARY XANTHURENIC ACID

1959 ◽  
Vol 5 (2) ◽  
pp. 45-50 ◽  
Author(s):  
A. S. ABBASSY ◽  
M. M. ZEITOUN ◽  
M. H. ABOUIWFA
Keyword(s):  
Vitamin B6 ◽  
The State ◽  
Xanthurenic Acid ◽  
Vitamin B6 Deficiency
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