Sialic Acid Content of Red Blood Cells from Protein-Calorie Malnourished Children and During Recovery, and from Normal Children and Adults

1973 ◽  
Vol 51 (11) ◽  
pp. 853-858
Author(s):  
C. Contreras ◽  
F. E. Viteri
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Recovery Period ◽  
Serum Vitamin ◽  
Sialic Acids ◽  
Red Cells ◽  
Control Group ◽  
Red Cell ◽  
Normal Children ◽  
Sialic Acid Content

Sialic acids were measured in the red cells of two groups of subjects. One group consisted of 12 children with severe protein-calorie malnutrition (P.C.M.); six of them were followed longitudinally throughout the recovery period. The control group included 28 normal children and 11 normal adults. All subjects were studied hematologically and the sialic acid content of the red cells was determined in three layers of erythrocytes, separated according to their density by ultracentrifugation. The results indicate that there are no alterations in the content of sialic acids in the red cells of children with severe P.C.M. Furthermore, they show that the sialic acid content of the red cell is not influenced by various levels of red cell folates nor by differences in the concentration of serum proteins, serum iron, percentage saturation of transferrin, serum folates, or serum vitamin B12.

scholarly journals Erythrocyte Sialic Acid Content during Aging in Humans: Correlation with Markers of Oxidative Stress

2012 ◽  
Vol 32 (3) ◽  
pp. 179-186 ◽  
Author(s):  
Mohammad Murtaza Mehdi ◽  
Prabhakar Singh ◽  
Syed Ibrahim Rizvi
Keyword(s):  
Oxidative Stress ◽  
Sialic Acid ◽  
Erythrocyte Membrane ◽  
Acid Content ◽  
Lipid Hydroperoxide ◽  
Plasma Lipid ◽  
Sialic Acids ◽  
Sialic Acid Content ◽  
Total Antioxidant ◽  
The Relationship

Sialic acids are substituted neuraminic acid derivatives which are typically found at the outermost end of glycan chains on the membrane in all cell types. The role of erythrocyte membrane sialic acids during aging has been established however the relationship between sialic acid and oxidative stress is not fully understood. The present work was undertaken to analyze the relationship between erythrocyte membrane sialic acid with its plasma level, membrane and plasma lipid hydroperoxide levels and plasma total antioxidant capacity. Results show that sialic acid content decreases significantly (P< 0.001) in RBC membrane (r= −0.901) and increases in plasma (r= 0.860) as a function of age in humans. Lipid peroxidation measured in the form of hydroperoxides increases significantly (P< 0.001) in plasma (r= 0.830) and RBC membranes (r= 0.875) with age in humans. The Trolox Equivalent Total Antioxidant Capacity (TETAC) of plasma was found to be significantly decreased (P< 0.001,r= −0.844). We observe significant correlations between decrease of erythrocyte membrane sialic acid and plasma lipid hydroperoxide and TETAC. Based on the observed correlations, we hypothesize that increase in oxidative stress during aging may influence the sialic acid decomposition from membrane thereby altering the membrane configuration affecting many enzymatic and transporter activities. Considering the importance of plasma sialic acid as a diagnostic parameter, it is important to establish age-dependent reference.

Lanthanum in Human Red Cell

1982 ◽  
Vol 40 ◽  
pp. 280-281
Author(s):  
Ezzatollah Keyhani ◽  
Eva Bland
Keyword(s):  
Sialic Acid ◽  
Ultrastructural Localization ◽  
Cellular Membrane ◽  
Red Cells ◽  
Red Cell ◽  
Total Lipids ◽  
Experimental Conditions ◽  
Sialic Acid Content ◽  
Biochemical Studies ◽  
Human Red Cells

The human red cell contains four major glycoproteins : sialoglycoprotein or PAS 1; Band III; PAS 2 and PAS 3. With the exception of Band III, all other glycoproteins contain high levels of sialic acid. In addition, human red cells contain glycolipids which have been estimated to be less than 5% of the total lipids. Lanthanum has been used as tracer of extracellular space and as a probe for studies of Ca++ metabolism across various types of cellular membrane. Biochemical studies led to the conclusion that lanthanum inhibition of Ca++ metabolism was related to the sialic acid content of the cell membrane. The purpose of this communication is to describe the ultrastructural localization of lanthanum in human red cells under various experimental conditions.

Removal of O-acetylated sialic acids from rat colonic epithelial glycoproteins by cell-free extracts of rat faeces

1983 ◽  
Vol 61 (8) ◽  
pp. 868-874 ◽  
Author(s):  
H. Poon ◽  
P. E. Reid ◽  
C. W. Ramey ◽  
W. L. Dunn ◽  
M. G. Clay
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Minimal Medium ◽  
Wistar Rat ◽  
Sialic Acids ◽  
Side Chain ◽  
Sialic Acid Content ◽  
Vibrio Cholera ◽  
Sterile Cell

Sterile, cell-free, extracts of freshly defaecated Wistar rat faeces in a pH 7.0 "minimal medium" contain neuraminidase(s), capable of removing sialic acids both with and without side-chain substituents from bovine submandibular mucin and rat colonic epithelial glycoproteins, and an esterase which removes O-acetyl substituents from the side chain of sialic acid residues. Studies of the removal of sialic acids from bovine submandibular mucin and rat colonic epithelial glycoproteins indicated that (i) the faecal enzymes removed a greater proportion of the sialic acid of both the de-O-acetylated and native glycoproteins than was removed with Vibrio cholera neuraminidase, (ii) sialic acids were removed more rapidly from de-O-acetylated glycoproteins, and (iii) the resistance to removal of sialic acids was apparently dependent at least in part upon the O-acetyl sialic acid content of the substrate.

Sialoglycoproteins and sialic acids ofPlasmodium knowlesischizont-infected erythrocytes and normal rhesus monkey erythrocytes

Parasitology ◽  
1986 ◽  
Vol 92 (3) ◽  
pp. 527-543 ◽  
Author(s):  
R. J. Howard ◽  
G. Reuter ◽  
J. W. Barnwell ◽  
R. Schauer
Keyword(s):  
Sialic Acid ◽  
Rhesus Monkey ◽  
Acid Content ◽  
Sialic Acids ◽  
Galactose Oxidase ◽  
Parasite Development ◽  
Sialic Acid Content ◽  
Acetylneuraminic Acid ◽  
Total Sialic Acid ◽  
Significant Difference

SUMMARYThe effects of malaria infection on RBC sialic acids and sialoglycoproteins were studied with asexual blood-stage infections ofPlasmodium knowlesiin rhesus monkeys. Glycoprotein radio-isotope labelling methods were used to compare the sialoglycoproteins of normal RBC andP. knowlesischizont-infected RBC (SI-RBC). Tritiation of glycoproteins from SI-RBC with the standard sialidase + galactose oxidase/NaB3H4method or standard periodate/NaB3H4method was significantly decreased when compared to normal RBC. However, tritium uptake into glycoproteins was normal when SI-RBC were treated with 5-fold higher concentrations of both enzymes in the first labelling method, or with a 5-fold increase in the molar ratio of periodate to sialic acid in the second method. The mobility of tritiated host cell glycoproteins on SDS–polyacrylamide gels was identical for SI-RBC and normal RBC. New bands, possibly glycoproteins, of 230, 160, 90, 52, and 30 kDa were detected after labelling SI-RBC by the modified periodate/NaB3H4method. Sialic acid analysis of normal rhesus monkey RBC (62μg/1010RBC) revealed that 46% of the total sialic acid wasN-glycolylneuraminic acid, 33% wasN-acetyl-9-O-acetylneuraminic acid, and the remainderN-acetylneuraminic acid. SI-RBC collected either directly from infected monkeys or afterin vitroculture of ring-infected RBC in horse serum, had increased total sialic acid (126 or 115μg/1010RBC, respectively). The sialic acid content of infected RBC must increase during parasite development since RBC infected with ring-stageP. knowlesihad the same content as normal RBC. There was no significant difference in the ratio of the three sialic acids of SI-RBC and normal RBC. In contrast, the uninfected RBC from infected blood of different monkeys showed marked variation in sialic acid composition and generally had a lower sialic acid content than normal RBC.

Sialic Acids in Human Gastric Aspirates: Detection of 9-O-Lactyl- and 9-O-Acetyl-N-Acetylneuraminic Acids and a Decrease in Total Sialic Acid Concentration with Age

1993 ◽  
Vol 84 (5) ◽  
pp. 573-579 ◽  
Author(s):  
A. P. Corfield ◽  
S. A. Wagner ◽  
A. Safe ◽  
R. A. Mountford ◽  
J. R. Clamp ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Thin Layer ◽  
Acid Content ◽  
Colorimetric Assay ◽  
Sialic Acids ◽  
Gas Chromatography Mass Spectrometry ◽  
Sialic Acid Content ◽  
Acetylneuraminic Acid ◽  
Total Sialic Acid ◽  
Thin Layer Chromatographic Analysis

1. The total sialic acid content of human gastric aspirates was measured using a colorimetric assay. Care was taken to optimize the assay and to eliminate interference. 2. The sialic acid content of gastric aspirates collected under resting conditions from 77 patients with non-ulcer dyspepsia was found to decrease with age from >100 μg/ml at 25 years and younger to <20 μg/ml above 70 years of age. 3. Analysis of the sialic acids by gas chromatography, mass spectrometry and thin-layer chromatography showed the presence of N-acetylneuraminic acid and two O-acylated derivatives, 9-O-acetyl- and 9-O-lactyl-N-acetylneuraminic acids. These forms were predominantly glycosidically bound. 4. Thin-layer chromatographic analysis of individual aspirate samples showed that the O-acetylated sialic acids were present in all samples, with a maximum of 25% and a minimum of 5% of the total sialic acids.

scholarly journals Protection through L-Carnitine on Tissue Oxidant Status and Sialic Acid Content in Tilmicosin-Induced Alterations in BALB/c Mice

2007 ◽  
Vol 76 (2) ◽  
pp. 203-207 ◽  
Author(s):  
A. Kart ◽  
M. Karapehlivan ◽  
K. Yapar ◽  
M. Citil ◽  
A. Akpinar
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Control Group ◽  
Sialic Acid Content ◽  
Tissue Samples ◽  
Total Sialic Acid ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2

The macrolide antibiotic tilmicosin is known to induce cardiotoxic effect when administered at large doses. In this work, the effects of tilmicosin were evaluated with respect to alterations in total sialic acid, malondialdehyde and glutathione content of the heart, liver, kidney and lung tissues after single subcutaneous injection of 75 mg/kg tilmicosin with or without L-carnitine (500 mg/kg for 5 days daily via s.c. route) in BALB/c mice. L-carnitine is a co-factor serving in the mitochondrial β-oxidation of long chain fatty acids, and it was reported to be protective in several types of toxicity cases probably via multi-factorial mechanisms. Twenty eight mice were divided into 4 groups including group 1 (control), group 2 (L-carnitine), group 3 (tilmicosin) and group 4 (tilmicosin plus L-carnitine). Following the administration of treatments, tissue samples were collected, and the samples were assayed for malondialdehyde, glutathione and total sialic acid content. Mice receiving tilmicosin treatment alone had significantly higher malondialdehyde and total sialic acid concentrations (except for MDA of lungs) but lower glutathione concentration in selected tissues compared to those of the control, group 2 (Carnitine only) and group 4 (L-carnitine plus tilmicosin) (p < 0.05). However, no significant difference was found associated with the assayed indicators between the control and mice treated with L-carnitine plus tilmicosin. These results suggest that tilmicosin may cause oxidative stress in the heart, liver, lung and kidneys, but the adverse effects could be attenuated by L-carnitine administration.

129: Sialic Acid Content of Urinary TAMM-Horsfall Protein is Reduced in Interstitial Cystitis Patients

2007 ◽  
Vol 177 (4S) ◽  
pp. 44-45
Author(s):  
C. Lowell Parsons ◽  
Mahadevan Rajasekaran ◽  
Marianne Chenoweth ◽  
Paul Stein
Keyword(s):  
Sialic Acid ◽  
Interstitial Cystitis ◽  
Acid Content ◽  
Sialic Acid Content
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