Lack of protective effect of oral enprostil, a synthetic prostaglandin E2, on intestinal transport and morphology following abdominal irradiation in the rat

1989 ◽  
Vol 67 (10) ◽  
pp. 1351-1356 ◽  
Author(s):  
A. B. R. Thomson ◽  
M. Keelan ◽  
M. T. Clandinin ◽  
M. Tavernini ◽  
T. Lam ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Single Dose ◽  
Surface Area ◽  
Intestinal Transport ◽  
Mucosal Surface ◽  
The Other ◽  
Intestinal Uptake ◽  
Mucosal Surface Area ◽  
Abdominal Irradiation

Previous studies have demonstrated that abdominal irradiation alters intestinal uptake of nutrients. The purpose of this study was to determine the effect of an orally administered synthetic prostaglandin E2, enprostil, given on three occasions shortly prior to a single exposure to 600 cGy external abdominal irradiation, on intestinal active and passive transport processes and villus morphology measured 7 days later. Animals were sham-irradiated (CONT) or were exposed to a single dose of 600 cGy external abdominal irradiation (RAD); two and one mornings before the day of irradiation or sham irradiation, and 1 h before irradiation or sham irradiation enprostil was administered. One half of CONT and RAD groups were dosed orally with enprostil, 5 μg/kg body weight, and the other half of the CONT and RAD groups were dosed with placebo. Seven days later the in vitro uptake of glucose, galactose, long-chain fatty acids, and cholesterol was determined in the four groups (CONT with and without enprostil, and RAD with and without enprostil). In CONT, enprostil was associated with increased jejunal uptake of glucose and ileal uptake of galactose. In RAD given enprostil, there was increased jejunal uptake of galactose but reduced ileal uptake of glucose and galactose. The expected radiation-associated decline in jejunal galactose uptake was prevented with enprostil. In CONT given enprostil, there was increased jejunal uptake of fatty acid (FA) 14:0 and 16:0 but reduced uptake of FA 18:0, 18:1, and 18:2; enprostil had no effect on lipid uptake in the ileum in CONT. Enprostil had a different effect in RAD, with reduced jejunal uptake of FA 14:0; 16:0, 18:0, 18:3, and cholesterol and reduced ileal uptake of FA 16:0, 18:1, 18:2, 18:3, and cholesterol. Enprostil was associated with a reduced jejunal mucosal surface area in CONT and a reduced ileal mucosal surface area in RAD. The irradiation-associated decline in body weight was not observed in animals given enprostil despite the lack of change in food intake. The alterations in nutrient uptake were not due to differences in food intake, weight change, or mucosal surface area. Thus, enprostil given on three occasions just before a single dose of abdominal irradiation was associated with increased intestinal uptake of galactose, but failed to prevent most of the other irradiation-associated changes in active and passive intestinal transport, and accentuated rather than prevented the irradiation-associated diminution in mucosal surface area.Key words: adaptation, cholesterol, fatty acids, galactose, glucose, ileum, jejunum.

Dietary effects of ω3-fatty acids on intestinal transport function

1988 ◽  
Vol 66 (7) ◽  
pp. 985-992 ◽  
Author(s):  
A. B. R. Thomson ◽  
M. Keelan ◽  
M. Garg ◽  
M. T. Clandinin
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fish Oil ◽  
Surface Area ◽  
Body Weight Gain ◽  
Intestinal Transport ◽  
Mucosal Surface ◽  
Variable Effect ◽  
Ω3 Fatty Acids ◽  
Mucosal Surface Area

Animals were fed for 2 weeks on one of four isocaloric and isocholesterolic semisynthetic diets: high 18:3ω3, low 18:3ω3, high 20:5ω3, or low 20:5ω3. The weight of the intestine and the percentage of the wall consisting of mucosa was greater in high 20:5ω3 than in high 18:3ω3, and greater in low 20:5ω3 than in low 18:3ω3, although the mucosal surface area was 26% lower in high 20:5ω3 than high 18:3ω3. The jejunal uptake of 40 mM glucose and ileal uptake of 40 mM galactose was greater in high 18:3ω3 than in high 20:5ω3; jejunal uptake of fatty acid 12:0 was higher, but 18:0 was lower in high 18:3ω3 than in high 20:5ω3. The jejunal or ileal uptake of cholesterol was not affected by 20:5ω3. However, 20:5ω3 had a variable effect on the uptake of medium- and long-chain fatty acids. Alterations in the uptake of fatty acids and glucose were not explained by any difference in the animals' food consumption, body weight gain, or intestinal weight, but the reduced jejunal uptake of 40 mM glucose in rats fed the high 20:5ω3 diet was associated with reduced mucosal surface area. Thus, (i) varying the source of ω3-fatty acids (vegetable, 18:3ω3 versus fish oil, 20:5ω3) altered the mucosal mass of the intestine, and (ii) the source of the dietary ω3-fatty acid (18:3ω3 versus 20:5ω3) influenced intestinal hexose uptake, with fish oil having an anti-absorptive effect on the jejunal uptake of D-glucose.

A high linoleic acid diet diminishes enhanced intestinal uptake of sugars in diabetic rats

1987 ◽  
Vol 252 (2) ◽  
pp. G262-G271 ◽  
Author(s):  
A. B. Thomson ◽  
M. Keelan ◽  
M. T. Clandinin ◽  
K. Walker
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Surface Area ◽  
Diabetic Rats ◽  
Intestinal Uptake ◽  
High Linoleic Acid ◽  
Acid Diet ◽  
Villous Surface ◽  
Linoleic Acid Diet ◽  
Mucosal Surface Area

Intestinal absorption is altered in rats by diabetes and by dietary manipulation. For 5 wk rats were fed chow (C) or semisynthetic diets containing isocaloric amounts of a high content of either polyunsaturated (P) or saturated (S) fatty acids; then half were rendered hyperglycemic with streptozotocin while the remaining half served as nondiabetic controls. Studies were performed after a total of 8 wk on C, P, or S. Jejunal villous surface area was greater in diabetic rats than in control rats fed C or S, whereas the jejunal mucosal surface area was significantly greater in diabetic animals than in control animals only when they were fed S. Ileal mucosal surface area was similar in diabetic and in control rats fed C, S, or P; although ileal villous surface area was greater in diabetic than in control rats only when they were fed C. The jejunal and ileal uptake of varying concentrations of glucose and galactose was higher in diabetic rats than controls fed C or S. In contrast, the enhanced uptake of these sugars was reduced or normalized in diabetic rats fed P. The jejunal brush-border membrane (BBM) content of phospholipids was higher in diabetic than in control rats fed chow, but there was no difference in diabetic vs. control animals fed P or S in BBM total free fatty acids, phospholipids, or cholesterol. Thus feeding a high linoleic acid diet diminishes the enhanced intestinal uptake of glucose and galactose in diabetic rats.(ABSTRACT TRUNCATED AT 250 WORDS)

Dietary lipids modify the age-associated changes in intestinal uptake of fructose in rats

2005 ◽  
Vol 288 (1) ◽  
pp. G125-G134 ◽  
Author(s):  
L. Drozdowski ◽  
T. Woudstra ◽  
G. Wild ◽  
M. T. Clandinin ◽  
A. B. R. Thomson
Keyword(s):  
Fatty Acids ◽  
Surface Area ◽  
Saturated Fatty Acids ◽  
Dietary Lipid ◽  
Dietary Lipids ◽  
Mucosal Surface ◽  
Elderly Age ◽  
Old Rats ◽  
Mucosal Surface Area

Because reduced nutrient absorption may contribute to malnourishment in the elderly, age and diet modulate fructose uptake in mice, and alterations in fructose uptake may be paralleled by changes in the abundance of fructose transporters, the objectives of this study were to determine 1) the effects of aging on fructose absorption in rats, 2) the effect of feeding diets enriched with saturated fatty acids (SFA) vs. polyunsaturated fatty acids (PUFA), and 3) the mechanisms of these age-and diet-associated changes. Male Fischer 344 rats aged 1, 9, and 24 mo received isocaloric diets enriched with SFA or PUFA. The uptake of 14C-labeled d-fructose was determined in vitro using the intestinal sheet method. Northern and Western blot analyses and immunohistochemistry were used to determine the abundance of sodium-independent glucose and fructose transporters (GLUT)2 and GLUT5. When expressed on the basis of mucosal surface area, jejunal fructose uptake was increased in 9 and 24 mo compared with 1-mo-old animals fed SFA. PUFA-fed animals demonstrated increased fructose uptake at 24 mo compared with younger animals. Ileal fructose uptake was increased with SFA vs. PUFA in 9-mo-old rats but was reduced with SFA in 1- and 24-mo-old rats. Variations in GLUT2 and GLUT5 abundance did not parallel changes in uptake. These results indicate that 1) age increases fructose uptake when expressed on the basis of mucosal surface area, 2) age influences the adaptive response to dietary lipid modifications, and 3) alterations in fructose uptake are not explained by variations in GLUT2 or GLUT5.

Fish oil prevents effect of high cholesterol diet on active intestinal transport of galactose

1989 ◽  
Vol 67 (8) ◽  
pp. 829-836 ◽  
Author(s):  
A. B. R. Thomson ◽  
M. Keelan ◽  
M. L. Garg ◽  
M. T. Clandinin
Keyword(s):  
Fatty Acids ◽  
Fish Oil ◽  
Surface Area ◽  
Beef Tallow ◽  
Mucosal Surface ◽  
High Cholesterol Diet ◽  
Long Chain Fatty Acids ◽  
Mucosal Surface Area ◽  
Long Chain ◽  
Chain Fatty Acids

We tested the hypothesis that diets containing fish oils prevent the effects of a high cholesterol diet on the morphology and nutrient uptake of the intestine. Isocaloric semisynthetic diets were supplemented with beef tallow or fish oil containing low or high amounts of cholesterol and were fed to growing female Wistar rats for 14 days, after which the in vitro jejunal and ileal uptake of glucose, galactose, long-chain fatty acids, and cholesterol was determined. Feeding cholesterol with beef tallow was associated with a 12% decrease in the jejunal mucosal surface area. Feeding fish oil decreased jejunal mucosal surface area by 24%, as compared with the beef tallow diet, but the reduction was increased to 42% when fish oil and cholesterol were fed together. Ileal surface area was unaffected by varying the major source of dietary lipid, or by adding cholesterol. Despite the effect of fish oil on the mucosal surface area, the jejunal and ileal uptake of saturated as well as unsaturated long-chain fatty acids and cholesterol was similar in the four diet groups. Cholesterol supplementation enhanced the jejunal uptake of high concentrations of galactose only when fed with beef tallow, i.e., feeding fish oil prevented the enhancing effect of cholesterol on galactose uptake observed when beef tallow was fed. Thus, (i) a fish oil diet prevents the enhancing effect of cholesterol on jejunal active transport of galactose, an effect not explained by the reduction in jejunal mucosal surface area observed with the fish oil diet; (ii) these dietary manipulations result in a clear dissociation of the morphological from the transport adaptation of the intestine; and (iii) substitution of fish oil for beef tallow as the major source of lipid in the diet prevents the influence of cholesterol on the active intestinal transport of galactose.Key words: adaptation, dietary cholesterol, galactose, glucose, long-chain fatty acids, permeation, fish oil.

Feeding diets containing 2% cheno- or urso-deoxycholic acid or cholestyramine to rats for two weeks alters intestinal morphology and bile acid absorption

1991 ◽  
Vol 69 (5) ◽  
pp. 592-598 ◽  
Author(s):  
M. Keelan ◽  
A. B. R. Thomson
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Bile Acid ◽  
Food Intake ◽  
Bile Acids ◽  
Surface Area ◽  
Mucosal Surface ◽  
Intestinal Morphology ◽  
Restricted Diet ◽  
Mucosal Surface Area

This study was undertaken to examine the effect of supplementing chow for 2 weeks with 2% cheno- (CDC) or ursodeoxycholic (UDC) acid or cholestyramine (CHOL) on the intestinal morphology and in vitro uptake of bile acids in adult rats. Food intake was higher in UDC and CHOL as compared with animals fed chow or CDC, or in animals pair-fed a chow-restricted diet (CRD). Body weight gain was lower in CDC and CRD but was unchanged by feeding UDC or CHOL. Jejunal mucosal surface area was similar in the five groups, although the ileal mucosal surface area was lower in UDC than in the other animals. Feeding UDC reduced the ileal uptake of cholic acid (C), taurocholic acid (TC), and glycocholic acid (GC). Feeding CDC had no effect on bile acid uptake except when compared with animals fed a chow-restricted diet. Feeding CHOL reduced the active ileal uptake of C, had no effect on the uptake of TC or GC or CDC, and was associated with increased uptake of stearic, linoleic, and linolenic acids. These effects were likely related to a direct effect of changes in the luminal bile acids rather than due to an indirect effect of the reduced food intake, since the ileal uptake of CDC and GC was greater in animals fed CDC than in those fed a chow-restricted diet with comparable weight gain. Thus, 2 weeks of feeding bile acids or bile acid binding agents may alter the form and function of the rat intestine, and as well may lead to changes in food intake and body weight gain.Key words: active transport, adaptation, bile acids.

scholarly journals Effect of Dietary Fat Composition and 2’Fucosyllactose on Neonatal Piglet Growth and Organ Lipid Composition

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1038-1038
Author(s):  
Michael Miklus ◽  
Pedro Prieto ◽  
Cynthia Barber ◽  
Robert Rhoads ◽  
Samer El-Kadi
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Fatty Acid ◽  
Body Weight Gain ◽  
Dietary Fatty Acids ◽  
The Body ◽  
The Other ◽  
Human Infant ◽  
Lps Challenge ◽  
Group 3

Abstract Objectives The objectives of this study were to determine the effect of 2’fucosyllactose (2’FL) and fat blends on growth, body composition and fatty acid profile of the liver and brain using the neonatal pig as a model for the human infant. Methods Pigs (3 d old) were randomly assigned to either: 1. control, 2. Palm Olein (PO) fat blend – Low 2'-FL, 3. PO – High 2'-FL, 4. High oleic acid (HO) – Low 2'-FL, 5. HO FB – High 2'-FL, 6. PO FB – GLA, or 7. kept with their sows. Pigs in groups 1 to 6 received 250 ml·kg−1·d−1 of formula in 5 equal meals for 15 d. On day 14 of the study, groups 1–6 received intraperitoneal E. coli LPS challenge at 100 µg·kg−1 weight. Results Body weight was greater for piglets fed by sows than those in the other groups (P < 0.001). In addition, % fat and bone mineral content were higher in the sow-fed group while lean % was less sow-fed piglets (group 7) compared with those in the other groups (P < 0.05). Only longissimus weight expressed as a % of body weight, was greater for group 7 compared with all other groups (P < 0.001). Soleus, semitendinosus, brain, heart and spleen weights as a % of body weight were similar across all groups. However, liver weight as a % of body weight was greater in groups 1–6 (3.7%) compared with group 7 (2.8%; P < 0.001). The proportion of brain 16:1 fatty acid was less (0.83%) for groups 1–6 than for group 7 pigs (1.08%; P < 0.0001). The proportion of 20:3 N6 was greatest (0.66%) for group 3 compared with groups 1 and 4 (0.55%; P < 0.05). In addition, the proportion of 20:5 N3 was greatest (0.12%) for group 3 compared with groups 1 and 7 (0.07%; P < 0.05). The proportion of liver 16:1, 18:0, and 18:1 cis-11 fatty acids were greater for group 7 (2.3, 23, 2.2%) than groups 1–6 (0.2, 20, 1.2%; P < 0.0001). Conversely, the contribution of 14:0, 18:1 cis-9, 18:3 N6 cis-6,9,12, and 22:6 N3 were greater for pigs in groups 1–6 (1.3, 0.6, and 14, 7.8%) compared with those in group 7 (0.5, 8.5, 0.2 and 3.5%; P < 0.0001). Conclusions Our data suggest that feeding 2’fucosyllactose had no effect on the body weight gain and composition in neonatal pigs. Our data also suggest that dietary fatty acids have a greater effect on liver than on brain fatty acid composition. Funding Sources Funding for the work was provided by Perrigo Nutritionals, LLC.

scholarly journals Spontaneous and experimental poisoning of cattle by Palicourea aeneofusca in the region of Pernambuco and induction of conditioned food aversion

2015 ◽  
Vol 46 (1) ◽  
pp. 138-143 ◽  
Author(s):  
Luiz Bezerra de Brito ◽  
Raquel Feitosa Albuquerque ◽  
Brena Pessoa Rocha ◽  
Samuel Salgado Albuquerque ◽  
Stephen Tomas Lee ◽  
...  
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Lithium Chloride ◽  
Test Group ◽  
Aversive Conditioning ◽  
Feeding Tube ◽  
The Other ◽  
Control Group ◽  
Food Aversion ◽  
Conditioned Food Aversion

ABSTRACT: The objective of this study was to describe the epidemiological, clinical, and pathological aspects of Palicourea aeneofusca poisoning in cattle in the region of Pernambuco, Brazil and to determine if it is possible to induce food aversion by P. aeneofusca poisoning in cattle raised under extensive management conditions. To determine the occurrence of poisoning, 30 properties were visited in five municipalities of the region of Pernambuco. Three outbreaks of poisoning of cattle were monitored. To induce conditioned food aversion by the consumption of P. aeneofusca, 12 animals were randomly distributed into two groups of six animals each. Cattle were weighed and received green P. aeneofusca leaves in their trough at a dose of 35mg kg-1 body weight for spontaneous consumption. The control group (CG) animals received water (1ml kg-1 body weight) via a feeding tube after the first ingestion of the plant, while the other animals, constituting the aversion test group (ATG), underwent induced aversion with lithium chloride (LiCl - 175mg kg-1 body weight) via a feeding tube. For the ATG cattle, the aversion to P. aeneofusca induced by a single dose of LiCl persisted for 12 months. In contrast, the CG animals continued to consume the plant in all tests performed, indicating the absence of aversion. This study showed that aversive conditioning using LiCl was effective in preventing poisoning by P. aeneofusca for a period of at least 12 months.

Effect of normal microbial flora on intestinal surface area

1961 ◽  
Vol 201 (1) ◽  
pp. 175-178 ◽  
Author(s):  
Helmut A. Gordon ◽  
Edith Bruckner-Kardoss
Keyword(s):  
Small Intestine ◽  
Young Adult ◽  
Surface Area ◽  
Mucosal Surface ◽  
Microbial Flora ◽  
Mucosal Surface Area ◽  
Normal Microbial Flora

The mucosal surface area of the small intestine was determined in young adult, germfree and conventional rats. On the average, the germfree values were found to be 30% lower than those of conventional animals. This reduction was pronounced in the mid- and lower parts of the small intestine and relatively slight in the upper segment. It is assumed that the higher surface area values of conventional rats are due to the "physiologic" stimulation by the normal microbial flora.

Seasonal changes in gut morphology of the striped field mouse (Apodemus agrarius)

1995 ◽  
Vol 73 (6) ◽  
pp. 1095-1099 ◽  
Author(s):  
Anetta Borkowska
Keyword(s):  
Small Intestine ◽  
Surface Area ◽  
Seasonal Changes ◽  
Poor Quality ◽  
Mucosal Surface ◽  
Apodemus Agrarius ◽  
Late Autumn ◽  
Field Mice ◽  
Highly Correlated ◽  
Mucosal Surface Area

I studied seasonal changes in length and wet mass of the digestive tract and area of the mucosal surface of the small intestine in wild adult striped field mice (Apodemus agrarius). The length and mass of the gut varied with season in males but not females. The mucosal surface area of the small intestine was highly correlated with seasonal variations in the size of the alimentary tract. Changes in the surface area of the mucosa depended on changes in both villus size and the number of villi per unit area. The area of the intestinal mucosa varied during the year in both females and males, but unexpectedly, the mucosal surface area in females was significantly larger than in males only in autumn. Because gut size decreased toward late autumn, I suggest that late autumn was not a stressful period (i.e., because of low temperature or poor-quality food) for wild field mice. The decrease in gut parameters may have been an effect of declining metabolic requirements and completion of reproduction in late autumn.

Renal response to vasopressin and indomethacin in cisplatin-treated rats

1987 ◽  
Vol 73 (4) ◽  
pp. 377-381 ◽  
Author(s):  
Christopher J. Lote ◽  
Ernest S. Harpur ◽  
Andrew Thewles ◽  
Donna J. Phipps
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Treated Group ◽  
Urine Flow ◽  
The Other ◽  
Control Group ◽  
Fischer 344 ◽  
Antidiuretic Effect ◽  
Natriuretic Effect ◽  
Observation Period

1. Cisplatin [6 mg/kg body weight, in 0.9% (w/v) NaCl] was injected intraperitoneally as a single dose to two groups of rats (Fischer 344 strain). Two further groups of rats, injected intraperitoneally with an equivalent volume of 0.9% (w/v) NaCl, were used as controls. The cisplatin-treated rats developed a pronounced polyuria which did not recover during an 18 week observation period. 2. After 21 weeks, one group of the cisplatin-treated animals received a 6 h infusion of 2.5% d-glucose. Vasopressin (60 μ-units min−1 100 g−1 body weight) was incorporated into the infusate for the final 2 h. A control group of animals received an identical infusion. One week later the other group of cisplatin-treated rats received a 6 h infusion of 0.9% (w/v) NaCl. Indomethacin was incorporated into the infusate for 15 min, at 3 h 52.5 min, to deliver a dose of 10 mg/kg body weight. A control group again received an identical infusion. 3. Cisplatin did not impair the antidiuretic effect of vasopressin, but it reduced the natriuretic effect of vasopressin, and also impaired the ability of the animals to produce concentrated urine. 4. Cisplatin did not alter basal PGE2 excretion, or the reduction in PGE2 excretion induced by indomethacin. However, the urine flow in the cisplatin-treated group did not fall after indomethacin, whereas there was a fall in urine flow in the control group.

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