A histochemical study of calcium storage in the foot of the freshwater gastropod, Helisoma duryi eudiscus (Pilsbry)

1968 ◽  
Vol 46 (5) ◽  
pp. 987-990 ◽  
Author(s):  
S. P. Kapur ◽  
M. A. Gibson
Keyword(s):  
Alkaline Phosphatase ◽  
Staining Intensity ◽  
Histochemical Demonstration ◽  
Staining Reaction ◽  
Connective Tissues ◽  
Intense Staining ◽  
Freshwater Gastropod ◽  
Helisoma Duryi ◽  
The Matrix ◽  
And Storage

Shortly after hatching, calcium appears in the form of numerous spherules within the connective tissue of the foot of Helisoma. Concomitantly, there is a change in the histochemical demonstration of sulfated mucopolysaccharides, glycogen, and alkaline phosphatase. The sulfated mucopolysaccharide component of the mucous glands and the mucous coating of the foot increase in staining intensity. Similarly, the glycogen content of the foot epithelium and subepithelial connective tissues increases in staining intensity. Also, alkaline phosphatase first appears and exhibits an intense staining reaction within the foot epithelium. It is suggested that the coincidental appearance of these substances is related to the percutaneous absorption and storage of calcium. It is proposed that the sulfated mucopolysaccharides absorb calcium from the environment, that this calcium–mucous complex is hydrolyzed by the alkaline phosphatase, that the released calcium becomes bound to the fibers and sulfated mucopolysaccharides forming the matrix of the spherules, and that the calcium is accumulated in the form of such spherules.

Relationship Between Meniscal Sample Swelling and the Compressive Properties of Bovine Menisci

2013 ◽  
Author(s):  
Stephen H. J. Andrews ◽  
Nigel G. Shrive ◽  
Janet L. Ronsky
Keyword(s):  
Vertical Direction ◽  
Axial Direction ◽  
Solid Matrix ◽  
Compressive Properties ◽  
Connective Tissues ◽  
Drag Forces ◽  
The Matrix ◽  
Two Phases ◽  
And Storage ◽  
Phosphate Buffered Saline

The menisci are anisotropic hydrated connective tissues, situated in the tibiofemoral joint. The menisci transmit approximately 50% of the load across this joint [1, 2]. In this tissue, compression would only be experienced in the axial (vertical) direction, and as such, many studies have tested samples in the axial direction to determine the compressive properties [3–5]. The material behaviour of the menisci has been described as biphasic, meaning the response of the tissue to applied load is time dependent and determined by both the solid constituents and their interaction with the fluid component [3]. Due to the low permeability of the tissue, deformation results in relative movement of the solid matrix and the fluid it contains, resulting in the creation of drag forces between the two phases. Fluid exudation from the matrix governs the viscoelastic behaviour of the tissue, including stress relaxation and creep [6]. The swelling behaviour of meniscal samples in varying osmotic environments was evaluated in our lab (unpublished data), where they swelled significantly, approximately 30% volumetrically in iso-osmotic phosphate buffered saline (PBS). It was hypothesized that the material properties of the tissue would be affected by this significant swelling. To date, no study has evaluated the effect of sample swelling, due to sample preparation and storage, on the behaviour of the menisci in compression. Therefore, the purpose of this study was to evaluate this relationship. We hypothesized that meniscal samples would be less stiff and more permeable in a swollen state than when they are compressed to the ‘fresh’, non-swollen, thickness prior to initiation of the protocol.

A histochemical study of the development of the mantle-edge and shell in the freshwater gastropod, Helisoma duryi eudiscus (Pilsbry)

1968 ◽  
Vol 46 (3) ◽  
pp. 481-491 ◽  
Author(s):  
S. P. Kapur ◽  
M. A. Gibson
Keyword(s):  
Alkaline Phosphatase ◽  
Ribonucleic Acid ◽  
Ground Substance ◽  
Freshwater Gastropod ◽  
Helisoma Duryi ◽  
Yellow Body ◽  
Golgi Element ◽  
Mantle Edge ◽  
Extrapallial Fluid ◽  
Distal Border

Glycogen and ribonucleic acid are present in the mantle-edge during the prehatching period and in the adult. The cubocolumnar epithelium contains the largest stores of glycogen. Ribonucleic acid is most abundant in the mantle-edge gland, and the mucous and mucoprotein cells. Mucopolysaccharides and glycoproteins occur within the mantle-edge epithelium, excepting the mantle-edge gland, and within the shell ground substance. Mucous glands and the sheath surrounding the organic plates are rich in sulfated mucopolysaccharides. Alkaline phosphatase and calcium could not be demonstrated during the prehatching stages. In the adult, alkaline phosphatase reactions are intense along the distal border of the cubocolumnar epithelium, and the basal borders of the epithelia of the mantle-edge gland, median lobe, and ventral lobe. Calcium carbonate occurs as spherules in the connective tissue, in the extrapallial fluid, and within the organic plates and crystalline layers of the shell. In the adult, lipids are most plentiful in the dorsal lobe epithelium and yellow body cells. Vitamin A occurs only within the cubocolumnar and yellow body cells. Cytochrome oxidase is present within the mantle-edge epithelium and, in terms of relative amounts, reflects the activity of the various lobes. Similarly, the size of the Golgi element can be correlated with the activity of the mantle-edge epithelia.

scholarly journals THE EFFECTS OF ALDEHYDE FIXATION ON ACID PHOSPHATASE ACTIVITY IN TISSUE BLOCKS

1965 ◽  
Vol 13 (6) ◽  
pp. 476-483 ◽  
Author(s):  
DAVID T. JANIGAN
Keyword(s):  
Acid Phosphatase ◽  
Aldehyde Group ◽  
Histochemical Demonstration ◽  
Histochemical Staining ◽  
Staining Reaction ◽  
Intense Staining ◽  
The Third ◽  
Enzyme Recovery ◽  
Liver And Kidney ◽  
Soluble State

The effect of various aldehydes on phosphatase(s) of rat liver and kidney hydrolyzing the monophosphates of β-glycerol, phenol, p-nitrophenol and naphthol AS-TR at pH 5.0 was determined. Biochemical data were correlated with histochemical staining results. On the basis of enzyme recovery after fixation, the aldehydes could be divided into 3 broad groups: (1) well over 50 per cent after hydroxyadipaldehyde and glyoxal; (2) near 50 per cent after formaldehyde and methacrolein; and (3) well below 50 per cent after crotonaldehyde, glutaraldehyde, and acrolein. Acid phosphatase(s) hydrolyzing all 4 substrates showed a similar differential response to fixation by 4 aldehydes, but individual recovery values with naphthol AS-TR phosphate were distinctly lower. Tissues immersed in the first aldehyde group were soft, showed poor morphologic preservation, and a large percentage of the activity was in a soluble state; these features were reflected in the histochemical demonstration of acid phosphatase by an intense staining reaction which was judged to be unsatisfactory. The reverse held true for tissues fixed in aldehydes of the third group, using glutaraldehyde in the staining tests Evidence is presented suggesting that acid phosphatase staining results after formaldehyde fixation is dependent in part on the duration of fixation.

scholarly journals ras Oncogene p21 expression is increased in premalignant lesions and high grade bladder carcinoma.

1985 ◽  
Vol 161 (5) ◽  
pp. 1213-1218 ◽  
Author(s):  
M V Viola ◽  
F Fromowitz ◽  
S Oravez ◽  
S Deb ◽  
J Schlom
Keyword(s):  
Bladder Carcinoma ◽  
Premalignant Lesions ◽  
Low Grade ◽  
Staining Reaction ◽  
High Grade ◽  
Ras Oncogene ◽  
Intense Staining ◽  
Bladder Urothelium ◽  
Normal Bladder ◽  
Ras P21

ras Oncogene p21 antigen is present in the most superficial cells of the normal bladder urothelium, as demonstrated by immunohistochemical staining. The pattern and intensity of p21 staining of cells in epithelial hyperplasia and low grade bladder carcinoma were similar to that seen in the normal urothelium. In contrast, epithelial cells in "premalignant" (dysplastic) lesions and high grade carcinomas exhibited an intense staining reaction for p21 antigen. ras p21 may be a useful marker for the malignant potential of both premalignant lesions and carcinomas of the bladder.

scholarly journals Proteins of the canine seminal plasma

2016 ◽  
Vol 46 (5) ◽  
pp. 901-908 ◽  
Author(s):  
Annice Aquino-Cortez ◽  
Lúcia Daniel Machado da Silva ◽  
Airton Alencar de Araújo ◽  
Erika da Silva Bezerra de Menezes ◽  
Arlindo de Alencar Araripe Noronha Moura
Keyword(s):  
Alkaline Phosphatase ◽  
Seminal Plasma ◽  
Binding Proteins ◽  
Reproductive Tract ◽  
Sperm Quality ◽  
Zinc Binding ◽  
Heparin Binding ◽  
Reproductive Disorders ◽  
The Matrix ◽  
Peroxidase Enzymes

ABSTRACT: Studies have been performed to identify the proteins present in canine seminal plasma (SP) and relate them to sperm quality as well as to discover molecular markers of reproductive tract diseases. There is evidence that heparin-binding proteins, zinc-binding proteins, and lactoferrin as well as the matrix metalloproteinase, superoxide dismutase, catalase, and glutathione peroxidase enzymes are associated with canine sperm quality. Other studies indicate that prolactin and enzymes like arginine esterase, acid phosphatase, and alkaline phosphatase could be successfully used as biomarkers of reproductive disorders. Thus, the present literature review aims to address aspects related to proteins of the canine SP, their influence on fertility, and their importance as biomarkers of reproductive disorders.

Histochemical studies of dopa oxidase and peroxidase in the mantle-edge of the freshwater gastropod, Helisoma duryi eudiscus (Pilsbry)

1968 ◽  
Vol 46 (2) ◽  
pp. 165-167 ◽  
Author(s):  
S. P. Kapur ◽  
M. A. Gibson
Keyword(s):  
Positive Reaction ◽  
Protein Component ◽  
Negative Reaction ◽  
Structural Proteins ◽  
Freshwater Gastropod ◽  
Helisoma Duryi ◽  
Mantle Edge ◽  
Dopa Oxidase ◽  
Oxidase Reaction

The mantle-edge gland produces the highly tanned, densely fibrous periostracum, and the cubocolumnar cells contribute to the deposition of the less highly tanned matrix of the inner shell layers. The mantle-edge gland gives positive reactions for dopa oxidase and peroxidase, but does not contain melanin. The cubocolumnar cells reveal a positive reaction for dopa oxidase, possess numerous melanin granules, and exhibit a negative reaction for peroxidase. It is suggested that quinones may contribute to the process of tanning and hardening of the structural proteins of the shell. The dopa oxidase reaction within the cubocolumnar cells indicates the presence of tyrosine and suggests that these cells are capable of producing quinones to color and harden the protein component of the inner shell layers. It also explains the abundance of melanin granules within these cells. Within the mantle-edge gland, it is suggested that the peroxidase inhibits the formation of melanin from dopa quinone, and peroxidase, by accentuating quinone production, may cause further hardening of the periostracum.

ANALYSIS OF PROTEOGLYCAN GENE EXPRESSION IN CONNECTIVE TISSUES BY SEMI-QUANTITATIVE RT-PCR

2001 ◽  
Vol 05 (02) ◽  
pp. 79-88
Author(s):  
K. Dobra ◽  
A. Hjerpe
Keyword(s):  
Rt Pcr ◽  
Connective Tissues ◽  
Surrounding Matrix ◽  
Cell Proliferation And Differentiation ◽  
Extracellular Matrix Components ◽  
Proliferation And Differentiation ◽  
The Matrix ◽  
Quantitative Changes ◽  
Regulatory Functions ◽  
Multiple Samples

Proteoglycans (PGs) are cell-membrane and extracellular matrix components with a wide variety of different functions. In the matrix, they are mainly of structural importance, although some of them have been ascribed specific regulatory functions, such as in the assembly of collagen fibers. PGs on the cell surface act as essential modulators of specific ligand-binding reactions, involving interactions between adjacent cells and between cells and surrounding matrix. Through these interactions they participate in different processes, including cell proliferation and differentiation. Qualitative and quantitative changes in PG expression can therefore be associated with various physiological and pathological conditions. We have optimized the conditions for semi-quantitative evaluation of proteoglycan expression by RT-PCR reaction, using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as reference gene. The relative fluorescence of analyte to reference amplimers can — within certain limits — be used to estimate the amount of target RNA and allows direct comparison of multiple samples. The profile of PG expression obtained in this way can be used to extend our current understanding of the possible functions that can be associated with these complex molecules.

scholarly journals 169 CULTURE OF MURINE EMBRYONIC STEM CELLS ON NWPF DISCS

2005 ◽  
Vol 17 (2) ◽  
pp. 235 ◽  
Author(s):  
G. Cetinkaya ◽  
S. Arat ◽  
H. Odaman Mercan ◽  
M.A. Onur ◽  
A. Tumer
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Es Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Signalling Pathways ◽  
Feeder Layers ◽  
The Matrix ◽  
Matrix Bound

Murine embryonic stem cells derived from the inner cell mass of mouse blastocysts can be maintained in culture for extended periods by using feeder layers and leukemia inhibitory factor (LIF). Maintenance of undifferentiated status occurs via LIF-mediated signalling pathways. In this study we cultured embryonic stem (ES) cells in Knockout-DMEM with serum replacement on a three-dimensional matrix, non-woven polyester fabric (NWPF), which is formed from non-arrayed polyethylene teraphthalate fibers. The surface of the fibers was modified by immobilizing LIF. While stimulating the matrix-bound form of LIF in vitro, we also tried to induce LIF-mediated signalling pathways continually. Our goal was to constitute a synthetic microenvironment that would support the undifferentiated growth of murine ES cells. Experimental groups were examined according to colony morphology, alkaline phosphatase activity, SSEA-1 antibody immunoreactivity, and SEM analyses. It was shown that three dimensional macroporous fibrous matrix, NWPF could support growth of undifferentiated ES cells. However, the ratio of undifferentiated colonies was higher on feeder layers than an polymeric surfaces (93% on mouse embryonic fibroblasts; 63,7% on hydrolized polymeric surface, P < 0,05). Results showed that LIF-immobilized surfaces supported undifferentiated growth of ES cells better than hydrolyzed surfaces. Colonies cultured on LIF-immobilized surfaces, had higher alkaline phosphatase activity and undifferentiated phenotype ratio than those on hydrolyzed surfaces. When the soluble or the matrix-bound form of LIF was used, the number of undifferentiated colonies increased in the polymeric groups (77.8% soluble LIF; 81.6% matrix bound LIF P < 0,05). On NWPF discs, ES cells formed big cell aggregates which had high alkaline phosphatase activity but low SSEA-1 immunoreactivity . When they were passaged to feeder layers, SSEA-1 activity increased. We managed to obtain undifferentiated colonies on NWPF discs by using LIF but the skeletal structure of polymeric matrix would be more convenient for differentiation studies. This study was performed in TUBITAK-RIGEB and supported by a part of grant from Hacettepe University (0102601001).

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