Inhibition of Mast Cell-Mediated Anaphylaxis by Sochungryong-Tang

2000 ◽  
Vol 28 (01) ◽  
pp. 69-76 ◽  
Author(s):  
H. M. Kim ◽  
G. S. Yoon ◽  
J. U. Seo ◽  
G. Moon ◽  
H. R. Kim ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Anaphylactic Shock ◽  
Mast Cell Degranulation ◽  
Dependent Manner ◽  
Asian Philosophy ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells ◽  
Anti Body ◽  
Dose Dependent

According to traditional Asian philosophy, Sochungryong-Tang (S-Tang) is a prescription for treating exterior syndrome. In this study, we investigated the effect of S-Tang on mast cell-mediated anaphylaxis. S-Tang completely inhibited compound 48/80-induced systemic anaphylactic shock at a dose of 100 mg/kg. When S-Tang was given as pretreatment at concentrations ranging from 1 to 1000 mg/kg, the serum histamine levels induced by compound 48/80 were reduced in a dose-dependent manner. S-Tang inhibited the local anaphylaxis activated by anti-dinitrophenyl (DNP) IgE anti-body, and also inhibited the histamine release from the rat peritoneal mast cells by compound 48/80 or anti-DNP IgE. These results indicate that S-Tang may contain substances with actions that inhibit mast cell degranulation.

scholarly journals Rat peritoneal mast cells release dipeptidyl peptidase II

1986 ◽  
Vol 236 (1) ◽  
pp. 215-219 ◽  
Author(s):  
G Struckhoff ◽  
E Heymann
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Substance P ◽  
Peritoneal Lavage ◽  
Dipeptidyl Peptidase ◽  
Dependent Manner ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells ◽  
Dose Dependent

Purified rat peritoneal mast cells have a 10-20-fold higher dipeptidyl peptidase II (DPP II) activity as compared with that of macrophages from the same source. Upon stimulation with the secretagogue Compound 48/80, DPP II is released from peritoneal-lavage cells and from purified mast cells, but not from purified macrophages, in a dose-dependent manner. Maximally, about one-third of the DPP II present in peritoneal-lavage cells is released. Substance P and the antigen/IgE system probably produce a similar effect. Both histamine and Zn2+, two ingredients of mast-cell granules, strongly inhibit DPP II at concentrations reported to occur in the granules. A possible role of mast-cell DPP II in the remodelling of connective tissue is discussed.

Effect of Alpinia oxyphylla Fruit Extract on Compound 48/80-induced Anaphylactic Reactions

2001 ◽  
Vol 29 (02) ◽  
pp. 293-302 ◽  
Author(s):  
Tae Yong Shin ◽  
Jin Hee Won ◽  
Hyung Min Kim ◽  
Sand Hyun Kim
Keyword(s):  
Mast Cells ◽  
Anaphylactic Shock ◽  
Dependent Manner ◽  
Basal Cells ◽  
Plasma Histamine ◽  
Fruit Extract ◽  
Alpinia Oxyphylla ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells ◽  
Dose Dependent

The effect of the aqueous extract of Alpinia oxyphylla Miq. (Zingiberaceae) fruits (AOFE) on anaphylactic reaction was investigated. AOFE completely inhibited compound 48/80-induced systemic anaphylactic shock at dose of 1.0 g/kg. When AOFE was pretreated at concentrations ranging from 0.01 to 1.0 g/kg, the plasma histamine levels induced by compound 48/80 were reduced in a dose-dependent manner. AOFE also inhibited the histamine release from rat peritoneal mast cells (RPMC) by compound 48/80. The level of cAMP in RPMC, when AOFE was added, transiently and significantly increased about 4-fold compared with that of basal cells. These results indicate that AOFE may be beneficial in the treatment of non-specific anaphylactic reactions.

Mast Cell Stabilizing, Antianaphylactic and Bronchodilatory activity of Methanolic extract of Averrhoa carambola Fruit

2021 ◽  
pp. 5258-5263
Author(s):  
Ravindra Babu Sajja ◽  
Prasad Konduri ◽  
Eswar Kumar Kilari
Keyword(s):  
Mast Cell ◽  
Methanolic Extract ◽  
Mast Cell Degranulation ◽  
Dependent Manner ◽  
Averrhoa Carambola ◽  
Peritoneal Mast Cells ◽  
Antianaphylactic Activity ◽  
Phytochemical Studies ◽  
Dose Dependent ◽  
Mast Cell Stabilization

This work was mainly aimed to study the mast cell stabilizing, anti-anaphylactic and bronchodilatory activities of methanolic extract of Averrhoa carambola (ACME). Mast cell stabilization activity was investigated by Compound 48/80 induced mast cell degranulation in rats and antianaphylactic activity was performed by determining the mortality rate of mice upon exposure to compound 48/80. The bronchodilatory effect of ACME was studied on histamine aerosol-induced bronchospasm using guinea pigs, in which occurrence of preconvulsive dyspnea (PCD) was noted as end point. Treatment with ACME (100, 200 and 400mg/kg) showed significant (p<0.05) protection of rat peritoneal mast cells and significantly (p<0.05) reduced the mortality of mice in a dose dependent manner. ACME significantly (p<0.05) increased the time of preconvulsive dyspnea (PCD) in a dose dependent manner that suggestive of bronchodilating activity. Phytochemical studies observed presence of saponins, tannins, steroids, alkaloids, flavonoids and glycosides. From these finding, we concluded that ACME possesses mast cell stabilizing; anti anaphylactic and bronchodilatory activity which might be used in treatment of asthma.

Dural mast cell degranulation is a putative mechanism for headache induced by PACAP-38

Cephalalgia ◽  
2012 ◽  
Vol 32 (4) ◽  
pp. 337-345 ◽  
Author(s):  
Michael Baun ◽  
Martin Holst Friborg Pedersen ◽  
Jes Olesen ◽  
Inger Jansen-Olesen
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Adenylate Cyclase ◽  
Phospholipase C ◽  
Dura Mater ◽  
Mast Cell Degranulation ◽  
Peritoneal Mast Cell ◽  
Selective Blockade ◽  
Peritoneal Mast Cells

Background: Pituitary adenylate cyclase activating peptide-38 (PACAP-38) has been shown to induce migraine in migraineurs, whereas the related peptide vasoactive intestinal peptide (VIP) does not. In the present study we examine the hypothesis that PACAP-38 and its truncated version PACAP-27 but not VIP cause degranulation of mast cells in peritoneum and in dura mater. Methods: The degranulatory effects of PACAP-38, PACAP-27 and VIP were investigated by measuring the amount of N-acetyl-β-hexosaminidase released from isolated peritoneal mast cells and from dura mater attached to the skull of the rat in vitro. In peritoneal mast cells N-truncated fragments of PACAP-38 (PACAP(6–38), PACAP(16–38) and PACAP(28–38)) were also studied. To investigate transduction pathways involved in mast cell degranulation induced by PACAP-38, PACAP-27 and VIP, the phospholipase C inhibitor U-73122 and the adenylate cyclase inhibitor SQ 22536 were used. Results: The peptides induced degranulation of isolated peritoneal mast cells of the rat with the following order of potency: PACAP-38 = PACAP(6–38) = PACAP(16–38) » PACAP-27 = VIP = PACAP(28–38). In the dura mater we found that 10−5 M PACAP-38 was significantly more potent in inducing mast cell degranulation than the same concentration of PACAP-27 or VIP. Inhibition of intracellular mechanisms demonstrated that PACAP-38-induced degranulation is mediated by the phospholipase C pathway. Selective blockade of the PAC1 receptor did not attenuate degranulation. Conclusion: These findings correlate with clinical studies and support the hypothesis that mast cell degranulation is involved in PACAP-induced migraine. PACAP-38 has a much stronger degranulatory effect on rat peritoneal and dural mast cells than VIP and PACAP-27. The difference in potency between PACAP-38- and PACAP-27/VIP-induced peritoneal mast cell degranulation is probably not related to the PAC1 receptor but is caused by a difference in efficacy on phospholipase C.

scholarly journals Cross talk between polysulfide and nitric oxide in rat peritoneal mast cells

2016 ◽  
Vol 310 (11) ◽  
pp. C894-C902 ◽  
Author(s):  
Amira Moustafa ◽  
Yoshiaki Habara
Keyword(s):  
Nitric Oxide ◽  
Mast Cells ◽  
Ryanodine Receptor ◽  
Cross Talk ◽  
Receptor Blocker ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Peritoneal Mast Cells ◽  
Intracellular Ca ◽  
Rat Peritoneal Mast Cells

The aim of this study was to define the effects of polysulfide on intracellular Ca2+ concentration ([Ca2+]i) and the underlying machinery, especially from the hydrogen sulfide (H2S) and nitric oxide (NO) perspectives, in rat peritoneal mast cells. We found that a polysulfide donor, Na2S4, increased [Ca2+]i, which is both extracellular and intracellular Ca2+ dependent. Intracellular Ca2+ release induced by Na2S4 was attenuated by the addition of a ryanodine receptor blocker. A slow-releasing H2S donor, GYY4137, dose dependently increased [Ca2+]i that was independent from extracellular Ca2+ influx. The GYY4137-induced [Ca2+]i release was partially attenuated in the presence of the ryanodine receptor blocker. Both polysulfide and H2S donors increased the intracellular NO levels in DAF-2-loaded mast cells, which were abolished by an NO scavenger, cPTIO. Inhibition of NO synthase (NOS) significantly abolished the polysulfide- or H2S-donor-induced [Ca2+]i elevation in the absence of extracellular Ca2+. An NO donor, diethylamine (DEA) NONOate, increased [Ca2+]i in a concentration-dependent manner, in which both extracellular and intracellular Ca2+ are associated. At higher concentrations, the DEA NONOate-induced [Ca2+]i increases were attenuated in the absence of extracellular Ca2+ and by the addition of the ryanodine receptor blocker. H2S and NO dose dependently induced polysulfide production. Curiously, polysulfide, H2S, and NO donors had no effect on mast cell degranulation. Among synthases, cystathionine-γ-lyase, and neuronal NOS seemed to be the major H2S- and NO-producing synthases, respectively. These results indicate that polysulfide acts as a potential signaling molecule that regulates [Ca2+]i homeostasis in rat peritoneal mast cells via a cross talk with NO and H2S.

Clarithromycin Dose-Dependently Stabilizes Rat Peritoneal Mast Cells

Chemotherapy ◽  
2016 ◽  
Vol 61 (6) ◽  
pp. 295-303 ◽  
Author(s):  
Itsuro Kazama ◽  
Kazutomo Saito ◽  
Asuka Baba ◽  
Tomohiro Mori ◽  
Nozomu Abe ◽  
...  
Keyword(s):  
Mast Cell ◽  
Plasma Membrane ◽  
Mast Cells ◽  
Water Soluble ◽  
Patch Clamp Technique ◽  
Membrane Deformation ◽  
Whole Cell Patch Clamp ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells ◽  
First Time

Background: Macrolides, such as clarithromycin, have antiallergic properties. Since exocytosis in mast cells is detected electrophysiologically via changes in membrane capacitance (Cm), the absence of such changes due to the drug indicates its mast cell-stabilizing effect. Methods: Employing the whole-cell patch clamp technique in rat peritoneal mast cells, we examined the effects of clarithromycin on Cm during exocytosis. Using a water-soluble fluorescent dye, we also examined its effect on deformation of the plasma membrane. Results: Clarithromycin (10 and 100 μM) significantly inhibited degranulation from mast cells and almost totally suppressed the GTP-γ-S-induced increase in Cm. It washed out the trapping of the dye on the surface of mast cells. Conclusions: This study provides for the first time electrophysiological evidence that clarithromycin dose-dependently inhibits the process of exocytosis. The mast cell-stabilizing action of clarithromycin may be attributable to its counteractive effect on plasma membrane deformation induced by exocytosis.

scholarly journals Anti-immunoglobulin-induced histamine secretion by rat peritoneal mast cells studied by immunoferritin electron microscopy.

1975 ◽  
Vol 142 (2) ◽  
pp. 391-402 ◽  
Author(s):  
D Lawson ◽  
C Fewtrell ◽  
B Gomperts ◽  
M Raff
Keyword(s):  
Electron Microscopy ◽  
Mast Cell ◽  
Mast Cells ◽  
Histamine Release ◽  
Histamine Secretion ◽  
Calcium Dependent ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

We have used ferritin-conjugated divalent and monovalent anti-Ig antibodies to study simultaneously, histamine secretion and the ultrastructural distribution and redistribution of Ig receptors on rat peritoneal mast cells. We conclude that (a) divalent anti-Ig is required for both receptor redistribution and for calcium-dependent degranulation and histamine release, (b) divalent anti-Ig induces patching and pinocytosis but not capping of Ig molecules, (c) neither capping nor pinocytosis are required for triggering and if clustering is necessary, then less than 10 Ig molecules are required per cluster, and (d) degranulation (and histamine release) is not an all or none response of the mast cell.

scholarly journals CYTOFLUOROMETRIC STUDY OF MAST CELL POLYANIONS II. ADULT RAT PERITONEAL MAST CELLS REGENERATING AFTER POLYMYXIN TREATMENT

1969 ◽  
Vol 17 (1) ◽  
pp. 56-61 ◽  
Author(s):  
SAM L. MEYER ◽  
ALEX M. SAUNDERS
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Polymyxin B ◽  
Adult Rat ◽  
Peritoneal Mast Cells ◽  
Fetal Rats ◽  
Rat Peritoneal Mast Cells ◽  
Time Changes ◽  
Metachromatic Granules ◽  
And Storage

Mast cells with metachromatic granules are not detectable in rats after polymyxin-B sulfate treatment. The morphologic and staining characteristics of the cells that repopulate the peritoneal cavity resemble those of mast cells of fetal rats in their maturation sequence, except that, in the adult, the sequence requires at least 56 days. During this time changes occur in the competitive staining of mast cells with acridine orange-sodium chloride, indicating that polyanion synthesis and storage in the granules is a multiphasic phenomenon.

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