Effect of Alpinia oxyphylla Fruit Extract on Compound 48/80-induced Anaphylactic Reactions

2001 ◽  
Vol 29 (02) ◽  
pp. 293-302 ◽  
Author(s):  
Tae Yong Shin ◽  
Jin Hee Won ◽  
Hyung Min Kim ◽  
Sand Hyun Kim
Keyword(s):  
Mast Cells ◽  
Anaphylactic Shock ◽  
Dependent Manner ◽  
Basal Cells ◽  
Plasma Histamine ◽  
Fruit Extract ◽  
Alpinia Oxyphylla ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells ◽  
Dose Dependent

The effect of the aqueous extract of Alpinia oxyphylla Miq. (Zingiberaceae) fruits (AOFE) on anaphylactic reaction was investigated. AOFE completely inhibited compound 48/80-induced systemic anaphylactic shock at dose of 1.0 g/kg. When AOFE was pretreated at concentrations ranging from 0.01 to 1.0 g/kg, the plasma histamine levels induced by compound 48/80 were reduced in a dose-dependent manner. AOFE also inhibited the histamine release from rat peritoneal mast cells (RPMC) by compound 48/80. The level of cAMP in RPMC, when AOFE was added, transiently and significantly increased about 4-fold compared with that of basal cells. These results indicate that AOFE may be beneficial in the treatment of non-specific anaphylactic reactions.

Inhibition of Mast Cell-Mediated Anaphylaxis by Sochungryong-Tang

2000 ◽  
Vol 28 (01) ◽  
pp. 69-76 ◽  
Author(s):  
H. M. Kim ◽  
G. S. Yoon ◽  
J. U. Seo ◽  
G. Moon ◽  
H. R. Kim ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Anaphylactic Shock ◽  
Mast Cell Degranulation ◽  
Dependent Manner ◽  
Asian Philosophy ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells ◽  
Anti Body ◽  
Dose Dependent

According to traditional Asian philosophy, Sochungryong-Tang (S-Tang) is a prescription for treating exterior syndrome. In this study, we investigated the effect of S-Tang on mast cell-mediated anaphylaxis. S-Tang completely inhibited compound 48/80-induced systemic anaphylactic shock at a dose of 100 mg/kg. When S-Tang was given as pretreatment at concentrations ranging from 1 to 1000 mg/kg, the serum histamine levels induced by compound 48/80 were reduced in a dose-dependent manner. S-Tang inhibited the local anaphylaxis activated by anti-dinitrophenyl (DNP) IgE anti-body, and also inhibited the histamine release from the rat peritoneal mast cells by compound 48/80 or anti-DNP IgE. These results indicate that S-Tang may contain substances with actions that inhibit mast cell degranulation.

scholarly journals Rat peritoneal mast cells release dipeptidyl peptidase II

1986 ◽  
Vol 236 (1) ◽  
pp. 215-219 ◽  
Author(s):  
G Struckhoff ◽  
E Heymann
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Substance P ◽  
Peritoneal Lavage ◽  
Dipeptidyl Peptidase ◽  
Dependent Manner ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells ◽  
Dose Dependent

Purified rat peritoneal mast cells have a 10-20-fold higher dipeptidyl peptidase II (DPP II) activity as compared with that of macrophages from the same source. Upon stimulation with the secretagogue Compound 48/80, DPP II is released from peritoneal-lavage cells and from purified mast cells, but not from purified macrophages, in a dose-dependent manner. Maximally, about one-third of the DPP II present in peritoneal-lavage cells is released. Substance P and the antigen/IgE system probably produce a similar effect. Both histamine and Zn2+, two ingredients of mast-cell granules, strongly inhibit DPP II at concentrations reported to occur in the granules. A possible role of mast-cell DPP II in the remodelling of connective tissue is discussed.

scholarly journals Cross talk between polysulfide and nitric oxide in rat peritoneal mast cells

2016 ◽  
Vol 310 (11) ◽  
pp. C894-C902 ◽  
Author(s):  
Amira Moustafa ◽  
Yoshiaki Habara
Keyword(s):  
Nitric Oxide ◽  
Mast Cells ◽  
Ryanodine Receptor ◽  
Cross Talk ◽  
Receptor Blocker ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Peritoneal Mast Cells ◽  
Intracellular Ca ◽  
Rat Peritoneal Mast Cells

The aim of this study was to define the effects of polysulfide on intracellular Ca2+ concentration ([Ca2+]i) and the underlying machinery, especially from the hydrogen sulfide (H2S) and nitric oxide (NO) perspectives, in rat peritoneal mast cells. We found that a polysulfide donor, Na2S4, increased [Ca2+]i, which is both extracellular and intracellular Ca2+ dependent. Intracellular Ca2+ release induced by Na2S4 was attenuated by the addition of a ryanodine receptor blocker. A slow-releasing H2S donor, GYY4137, dose dependently increased [Ca2+]i that was independent from extracellular Ca2+ influx. The GYY4137-induced [Ca2+]i release was partially attenuated in the presence of the ryanodine receptor blocker. Both polysulfide and H2S donors increased the intracellular NO levels in DAF-2-loaded mast cells, which were abolished by an NO scavenger, cPTIO. Inhibition of NO synthase (NOS) significantly abolished the polysulfide- or H2S-donor-induced [Ca2+]i elevation in the absence of extracellular Ca2+. An NO donor, diethylamine (DEA) NONOate, increased [Ca2+]i in a concentration-dependent manner, in which both extracellular and intracellular Ca2+ are associated. At higher concentrations, the DEA NONOate-induced [Ca2+]i increases were attenuated in the absence of extracellular Ca2+ and by the addition of the ryanodine receptor blocker. H2S and NO dose dependently induced polysulfide production. Curiously, polysulfide, H2S, and NO donors had no effect on mast cell degranulation. Among synthases, cystathionine-γ-lyase, and neuronal NOS seemed to be the major H2S- and NO-producing synthases, respectively. These results indicate that polysulfide acts as a potential signaling molecule that regulates [Ca2+]i homeostasis in rat peritoneal mast cells via a cross talk with NO and H2S.

scholarly journals Nerve growth factor prevents apoptosis of rat peritoneal mast cells through the trk proto-oncogene receptor

Blood ◽  
1995 ◽  
Vol 86 (12) ◽  
pp. 4638-4644 ◽  
Author(s):  
K Kawamoto ◽  
T Okada ◽  
Y Kannan ◽  
H Ushio ◽  
M Matsumoto ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mast Cells ◽  
Inhibitory Activity ◽  
Tissue Type ◽  
G1 Phase ◽  
Dependent Manner ◽  
Nerve Growth ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

We investigated the inhibitory activity of nerve growth factor (NGF) on apoptosis of rat peritoneal mast cells (PMCs) and compared it with that of recombinant stem cell factor (rSCF), which is a mast cell growth factor. When PMCs were incubated up to 72 hours in the presence of control medium, internucleosomal fragmentation of DNA indicating apoptosis was detected by agarose gel electrophoresis and flow cytometry. The aged PMCs showed morphological changes typical for apoptosis, such as chromatin condensation and loss of microvilli of the cell membrane. Addition of NGF or rSCF prevented development of the characteristic DNA fragmentation and decreased the proportion of apoptotic cells with low DNA content values in a dose-dependent manner. Polyclonal antibody to NGF completely abolished the inhibitory activity of NGF but not of rSCF. NGF-induced PMCs were in the G0/G1 phase of the cell cycle, but rSCF transited them from the G0/G1 phase to the S/G2M phase, suggesting that NGF, unlike rSCF, may have no proliferation activity to PMCs. By flow cytometric analysis with antibodies to NGF receptors p75LNGFR and p140trk, we defined that PMCs expressed p140trk but not p75LNGFR. Addition of herbimycin A or K-252a, tyrosine kinase inhibitors, to NGF resulted in blockage of the NGF-induced p140trk phosphorylation and restriction of the inhibitory activity of NGF on apoptosis of PMCs. These results indicated that NGF suppressed apoptosis of rat PMCs through the p140trk tyrosine phosphorylation and possessed no proliferative activity. Thus, NGF may act as a key factor to promote survival of connective tissue-type mast cells.

scholarly journals A patch-clamp study of histamine-secreting cells.

1986 ◽  
Vol 88 (3) ◽  
pp. 349-368 ◽  
Author(s):  
M Lindau ◽  
J M Fernandez
Keyword(s):  
Mast Cells ◽  
Patch Clamp ◽  
Single Channel ◽  
Resting Potential ◽  
Dependent Manner ◽  
Patch Clamp Technique ◽  
Whole Cell ◽  
Peritoneal Mast Cells ◽  
Ionic Conductances ◽  
Rat Peritoneal Mast Cells

The ionic conductances in rat basophilic leukemia cells (RBL-2H3) and rat peritoneal mast cells were investigated using the patch-clamp technique. These two cell types were found to have different electrophysiological properties in the resting state. The only significant conductance of RBL-2H3 cells was a K+-selective inward rectifier. The single channel conductance at room temperature increased from 2-3 pS at 2.8 mM external K+ to 26 pS at 130 mM K+. This conductance, which appeared to determine the resting potential, could be blocked by Na+ and Ba2+ in a voltage-dependent manner. Rat peritoneal mast cells had a whole-cell conductance of only 10-30 pS, and the resting potential was close to zero. Sometimes discrete openings of channels were observed in the whole-cell configuration. When the Ca2+ concentration on the cytoplasmic side of the membrane was elevated, two types of channels with poor ion specificity appeared. A cation channel, observed at a Ca2+ concentration of approximately 1 microM, had a unit conductance of 30 pS. The other channel, activated at several hundred micromolar Ca2+, was anion selective and had a unit conductance of approximately 380 pS in normal Ringer solution and a bell-shaped voltage dependence. Antigenic stimulation did not cause significant changes in the ionic conductances in either cell type, which suggests that these cells use a mechanism different from ionic currents in stimulus-secretion coupling.

The Evaluation of Antianaphylactic Effect of Oryza sativa L. in Rats

1999 ◽  
Vol 27 (01) ◽  
pp. 63-71 ◽  
Author(s):  
Hyung Min Kim ◽  
Dong-Kue Yi ◽  
Hye Young Shin
Keyword(s):  
Oryza Sativa ◽  
Mast Cells ◽  
Histamine Release ◽  
Methanol Extract ◽  
Oryza Sativa L ◽  
Dependent Manner ◽  
Peritoneal Mast Cells ◽  
Dose Dependent

This study was carried out to examine the effect of methanol extract of Oryza sativa L. (Dong-Jin in Korean, abbreviate as Os-DJ hereafter) on anaphylaxis. Os-DJ (10-5 to 1 g/kg) dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in rats. When Os-DJ was pretreated at concentration ranging from 10-5 to 1 g/kg, the serum histamine levels were reduced in a dose-dependent manner. Os-DJ (1 g/kg) also significantly inhibited local anaphylaxis activated by anti-dinitrophenyl (DNP) IgE. Moreover, Os-DJ dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. These results indicate that Os-DJ possess anti anaphylactic activity by inhibition of histamine release from mast cells in vivo and in vitro.

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