Comparison of the Free Radical-scavenging Ability of Captopril and Ascorbic Acid in an In-vitro Model of Lipid Oxidation. Implications for Reperfusion Injury and ACE Inhibitor Therapy

Background: The assessment of underexploited leaves has become crucial to supplement the rapidly depleting sources of bioactive components as well as provide available nutrient sources for local inhabitants. Methods: This study thus investigated the bioactive components of the oil, and fatty acid composition, free radical scavenging potentials, and protein qualities of leaves of Z. mays and G. celosioides using standard methods. The bioactive components of the oils and fatty acids were determined by Gas Chromatograpy, while the amino acid and in-vitro antioxidant potentials were determined using a Technicon Sequential Multi-Sample (TSM) Amino Acid Analyzer, and spectrophotometer, respectively. Results: The Z. Mays leaves showed the abundance of farnesene, hexadecanoic acids, and caryophellene while G. celosioides produced high level of octadecadienoic acid, hexadecanoic acid, and phytol. Z. mays and G. celosioides contained 72.48% and 60.55% unsaturated fatty acids respectively, with the abundance of linolenic acid for Z. mays and oleic acid for G. celosioides. The result for the in vitro antioxidant % inhibition showed a concentration dependent free radical scavenging potentials of the leaves. Both G. celosioides and Z. mays produced greater 1,1-diphenyl-2- picrylhydrazyl (DPPH), and hydrogen peroxide radical scavenging potentials than ascorbic acid, while at 40ppm the nitric oxide and 2,2- azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical % inhibition of Z. mays leaves were lower than those for ascorbic acid. Discussion: The number of essential amino acids in both plants were 48.20 and 39.25 g/100g, total branched chain amino acids (TBCAA) were 21.15 and 16.92 g/100g, predicted protein efficiency ratios (P-PERs) were in the range of 3.02-3.23 and 2.68-2.77, and the essential amino acid index (EAAI) were 1.52 and 1.48, for Z. mays and G. celosioides leaves respectively. Conclusion: From these results, the utilization of Z. mays and G. celosioides for high quality protein, unsaturated fatty acids and potent antioxidant sources, should be massively encouraged.

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Synergistic Antioxidant Effects of Araloside A and L-Ascorbic Acid on H2O2-Induced HEK293 Cells: Regulation of Cellular Antioxidant Status

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/9996040 ◽

2021 ◽

Vol 2021 ◽

pp. 1-20

Author(s):

Yaqin Tian ◽

Xiuling Zhang ◽

Meiling Du ◽

Fengfeng Li ◽

Manyu Xiao ◽

...

Keyword(s):

Oxidative Stress ◽

Ascorbic Acid ◽

Free Radical ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Hek293 Cells ◽

Protein Carbonyls ◽

Food Ingredients ◽

Radical Scavenging Ability ◽

Antioxidant Effects

Araloside A is a pentacyclic triterpenoid saponin, and L-ascorbic acid is a globally recognized antioxidant. In this study, coadministered araloside A and L-ascorbic acid were found to have a strong synergistic antioxidant effect, and correlations between cellular antioxidant indexes and free radical scavenging ability were found. Individual and combined pretreatment with araloside A and L-ascorbic acid increased both cell viability and antioxidant enzyme activity and inhibited the release of lactate dehydrogenase (LDH); the accumulation of malondialdehyde (MDA), lipid peroxidation (LPO) products, and H2O2; and the production of intracellular reactive oxygen species (ROS), protein carbonyls, and 8-hydroxy-2-deoxy guanosine (8-OHdG). Free radical scavenging ability was positively correlated with superoxide dismutase (SOD) and catalase (CAT) activity, the glutathione (GSH)/oxidized glutathione (GSSG) ratio, and total antioxidant capacity (T-AOC). Our study is the first investigation of araloside A and L-ascorbic acid coadministration for the treatment of diseases caused by oxidative stress. The synergistic antioxidant effects of araloside A and L-ascorbic acid support their potential as functional food ingredients for the elimination of oxidative stress-induced adverse reactions.

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EVALUATION OF ANTIOXIDANT EFFECT OF ALCOHOLIC ROOTS EXTRACT OF EUPHORBIA HIRTA LINN. – IN VITRO

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i1.39906 ◽

2021 ◽

pp. 111-114

Author(s):

RASHMI WADHWA ◽

PANKAJ GUPTA

Keyword(s):

Ascorbic Acid ◽

Free Radical ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Total Phenolic ◽

Root Extract ◽

Alcoholic Extract ◽

Flavonoid Content ◽

Euphorbia Hirta

Objective: The main objective of the present study was to undergo an investigation on free radical scavenging activity of the roots of Euphorbia hirta Linn. and was compared with a standard antioxidant compound like ascorbic acid. Methods: Euphorbia hirta roots extract was tested for total flavonoid content, total phenolic content, and in vitro antioxidant activity by 1, 1-diphenyl- 2-picrylhydrazyl (DPPH) free radical scavenging assay (DPPH) assay, superoxide anion radical scavenging assay, and nitric oxide scavenging assay. Results: The alcoholic roots extract of E. hirta was screened for free radical scavenging and antioxidant activities using three different methods. It was found that percentage inhibition of the extract was concentration-dependent. Total phenolic content and total flavonoid content were found to be 265.72±1.3 and 45.67±1.14, respectively. The alcoholic extract of E. hirta produced a dose-dependent inhibition of superoxide radicals ranging from 40.14±0.14 to 70.93±0.10. The mean IC50 values for DPPH radical by root extract of E. hirta and ascorbic acid were found to be 18.12 μg/ml and 13.17 μg/ ml, respectively. The alcoholic extract of E. hirta produced dose-dependent inhibition of nitric oxide radicals scavenging effect ranging from 17.05±0.18 to 51.08±0.30. The alcoholic extract of E. hirta and ascorbic acid shows mean IC50 values for superoxide radical as 23.64 μg/ml and 14.36 μg/ml, respectively. Conclusions: The present study showed that E. hirta possesses a considerable amount of both phenolic and flavonoid content. The alcoholic root extract also shows good antioxidant potential. The results of the present study also encourage for further in vivo studies and isolation and characterization of active compounds.

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In Vitro Kinetic Evaluation of the Free Radical Scavenging Ability of Propofol

Anesthesiology ◽

10.1097/aln.0b013e3182567dcc ◽

2012 ◽

Vol 116 (6) ◽

pp. 1258-1266 ◽

Cited By ~ 15

Author(s):

Weiguang Li ◽

Yu Zhang ◽

Yanru Liu ◽

Feng Yue ◽

Yiming Lu ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Free Radical ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

The Body ◽

Kinetic Evaluation ◽

Working Solution ◽

Radical Scavenging Ability

Background Propofol is a widely used, short-acting, and intravenously administered hypnotic agent with notable antioxidant and free radical scavenging activities. However, there are relatively few kinetic studies on the free radical scavenging ability of propofol. The goal of this study is to evaluate the kinetics of propofol scavenging 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical (ABTS(·+)). Methods The stock solution of ABTS(·+) was prepared by incubating 7 mM ABTS with 2.8 mM potassium persulfate in deionized water, and then diluted with 5 mM phosphate-buffered saline (pH 7.2) to get a working solution (36 μM ABTS(·+) and 18 μM ABTS). The reaction was monitored by measuring specific absorbance changes of ABTS and ABTS(·+) after adding 4 μM propofol (final concentration) to the working solution. The propofol-ABTS(·+) reaction products were analyzed by high-performance liquid chromatography and liquid chromatography mass spectrometry/mass spectrometry. Results Wave scanning and kinetic evaluation demonstrated that the ABTS(·+) scavenging process of propofol is relatively fast. The ABTS(·+) consumption rate by propofol is greater than the rate of ABTS formation. The degradation products of reaction between propofol and ABTS(·+) were mainly ABTS-propofol, a part of the ABTS molecule, and a combination of propofol with a part of the ABTS molecule. Conclusions Propofol scavenges ABTS(·+) with a fast and stable kinetic feature in vitro, which is useful and important for understanding propofol's antioxidant properties. The kinetic process of the free radical scavenging activity of propofol may also play a role in dynamic protection in the body.

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Antioxidant Activity In Vitro Guided Screening and Identification of Flavonoids Antioxidants in the Extract from Tetrastigma hemsleyanum Diels et Gilg

International Journal of Analytical Chemistry ◽

10.1155/2021/7195125 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Li Ding ◽

Xiaomin Zhang ◽

Jiajia Zhang

Keyword(s):

Antioxidant Activity ◽

High Performance Liquid Chromatography ◽

Free Radical ◽

High Performance ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Tetrastigma Hemsleyanum ◽

High Antioxidant Activity ◽

Radical Scavenging Ability

This study aimed to investigate the extract with high antioxidant activity of Tetrastigma hemsleyanum Diels et Gilg and identify the antioxidant components in vitro. α, α-Diphenyl-β-picrylhydrazyl (DPPH) radical assay, Trolox equivalent antioxidant capacity (TEAC) assay, ferric reducing antioxidant power (FRAP), and hydroxyl radical scavenging method were used to screen the extract with high antioxidant activity. The antioxidant capacity of the extracts was evaluated by the free radical scavenging ability of DPPH. The ability of extracts to scavenge 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical was evaluated by TEAC assay. The FRAP method was used to evaluate the ability of extracts to reduce Fe3+. The ability to scavenge hydroxyl radicals produced by the interaction of hydrogen peroxide and Fe2+ was measured by monitoring the change in the absorbance of the reaction mixture at 536 nm. Then, high-performance liquid chromatography-DPPH (HPLC-DPPH) and HPLC-hydroxyl radical scavenging methods were used to screen the antioxidant components in the extract. The molecular weight of the above antioxidant components was investigated using the qualitative analytical method of high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-Q-TOF LC/MS). Based on the concentrations of the samples (0.2–4 mg/mL), the DPPH free radical scavenging ability, ABTS+ free radical scavenging ability, hydroxyl free radical scavenging ability, and Fe3+ reducing ability of the ethyl acetate extract (EAE) were stronger than that of the crude extract (CE), petroleum ether extract (PEE), and n-butanol extract (BE). The EAE has higher antioxidant activity than CE, PEE, and BE. Six antioxidant components, rutin, quercetin, isoquercetin, astragalin, kaempferol, and kaempferol-3-o-rutoside, were identified in the EAE.

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Neuroprotective Potential of Verbascoside Isolated from Acanthus mollis L. Leaves through Its Enzymatic Inhibition and Free Radical Scavenging Ability

Antioxidants ◽

10.3390/antiox9121207 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1207

Author(s):

Carmen Burgos ◽

Dolores Muñoz-Mingarro ◽

Inmaculada Navarro ◽

Carmen Martín-Cordero ◽

Nuria Acero

Keyword(s):

Free Radical ◽

Neurodegenerative Diseases ◽

Antioxidant Capacity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Neuroprotective Activity ◽

Ageing Population ◽

Enzymatic Inhibition ◽

Radical Scavenging Ability

The phenomenon of today’s ageing population has increased interest in the search for new active substances that delay the onset and development of neurodegenerative diseases. In this respect, the search for natural compounds, mainly phenolic compounds, with neuroprotective activity has become the focus of growing interest. Verbascoside is a phenylethanoid that has already presented several pharmacological activities. The purpose of this study is to isolate and identify verbascoside from Acanthus mollis leaves. Consequently, its neuroprotective ability through enzymatic inhibition and free radical scavenging ability has been analyzed both in vitro and in cell culture assays. The antioxidant capacity of verbascoside was evaluated in vitro through total antioxidant capacity, DPPH•, •OH, and O2•—scavenging activity assays. The effect of verbascoside on intracellular reactive oxygen species (ROS) levels of HepG2 and SH-SY5Y cell lines was studied in normal culture and under induced oxidative stress. The inhibitory ability of the phenylethanoid against several enzymes implied in neurodegenerative diseases (tyrosinase, MAO-A, and AChE) was analyzed in vitro. Verbascoside neuroprotective activity is at least in part related to its free radical scavenging ability. The effect of verbascoside on ROS production suggests its potential in the prevention of harmful cell redox changes and in boosting neuroprotection.

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PHYTOCHEMICAL ANALYSIS AND IN VITRO FREE RADICAL SCAVENGING ACTIVITIES OF MEDICAGO SATIVA SEEDS

Kongunadu Research Journal ◽

10.26524/krj82 ◽

2015 ◽

Vol 2 (1) ◽

pp. 128-132

Author(s):

Gomathi R ◽

Banu S ◽

Usha K

Keyword(s):

Ascorbic Acid ◽

Free Radical ◽

Medicago Sativa ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Phytochemical Analysis ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Free Radical Scavenging Activities

Phytochemical analysis and in vitro free radical scavenging activities were analyzed in the various extracts of Medicago sativa seeds. The phytochemical analysis showed the presence of alkaloids, carbohydrates, flavonoids, glycosides, saponins, phytosterols, tannins, terpenoids and phenols. Among the various extracts, phytochemicals were extracted best in ethanol. Free radical scavenging activities such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, superoxide, 2,2'-azino-bis (3-ethylbenzothiazoline-6- sulphonic acid) (ABTS), ferrous ion chleating activity and non radicals such as hydrogen peroxide and nitric oxide were analyzed in the various extracts of Medicago sativa seeds and were compared with standard antioxidant ascorbic acid. All the extracts of Medicago sativa seeds scavenged the free radicals in a concentration dependent manner. The antioxidative activity of all the extracts was found to be more pronounced than that of the standard antioxidant ascorbic acid. Among the various extracts, the antioxidant activity was found to be more pronounced in ethanolic extract of Medicago sativa seeds

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Novel cholinesterase inhibitory effect of α-spinasterol isolated from the leaves of Acacia auriculiformis A. CUNN Ex. Benth (Fabaceae)

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v19i7.20 ◽

2020 ◽

Vol 19 (7) ◽

pp. 1473-1479

Author(s):

Bilqis A. Lawal ◽

Aniefiok Udobre ◽

Taiwo O. Elufioye ◽

Augustine A. Ahmadu ◽

Bolatito Olanipekun

Keyword(s):

Ascorbic Acid ◽

Free Radical ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Inhibitory Effect ◽

Free Radical Scavenging ◽

Acacia Auriculiformis ◽

Ic50 Value ◽

Potent Inhibitory Effect

Purpose: To investigate the in vitro anticholinesterase, α-glucosidase and antioxidant activities of α-spinasterol isolated from Acacia auriculiformis leaves.Methods: The powdered leaves of Acacia auriculiformis were extracted with 70 % ethanol and the dried hydroalcoholic extract was suspended in water and partitioned with ethyl acetate and n-butanol to give their soluble fractions. The in vitro inhibitory activities of α-spinasterol were determined against cholinesterase and, α-glucosidase enzymes, and free radical scavenging potentials using (1,1-diphenyl-2-picrylhydarzyl (DPPH) and 2,2-azino-bis (3-Ethylbenzothiazoline-6-sulphonic acid (ABTS) antioxidantassays.Results: The compound, α-spinasterol, exhibited moderate anticholinesterase activity (IC50 value of 44.19±2.59 μg/mL which was significantly different at (p < 0.05) when compared to the standard galanthamine (IC50 value of 1.73 ± 1.10 μg/mL). It also displayed a good α-glucosidase inhibitory activity with IC``` value of 8.65 ± 1.71μg/mL which was not significantly different when compared to the standard, acarbose with IC50 value of 2.79±0.81 μg/mL. This compound, however, exhibited weak free radical scavenging activities at 26.93 ± 0.00 and 35.16 ±.0.26 % inhibition of DPPH+ and ABTS+ radicals as compared to ascorbic acid and Trolox (73.88 ± 0.04 and 99.82 ± 0.00%) respectively.Conclusion: The results show that α-spinasterol isolated from Acacia auriculiformis exerts potent inhibitory effect against cholinesterase enzyme which might serve as a lead in the search for drugs against Alzheimer disease and diabetes mellitus. Keywords: Acacia auriculiformis, α-Spinasterol, Galanthamine, Acarbose, Trolox, Ascorbic acid

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