Hepatoprotective Effect ofShidagonglaoon Acute Liver Injury Induced by Carbon Tetrachloride

2009 ◽  
Vol 37 (06) ◽  
pp. 1085-1097 ◽  
Author(s):  
Jung Chao ◽  
Meng-Shiou Lee ◽  
Sakae Amagaya ◽  
Jiunn-Wang Liao ◽  
Jin-Bin Wu ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Ethanol Extract ◽  
Neutrophil Infiltration ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Hepatoprotective Effect ◽  
Oxidative Enzymes ◽  
Tnf Α

This study investigates the hepatoprotective activity of ethanol extract from Shidagonglao roots (SDGLEtOH). The hepatoprotective effect of SDGLEtOH(20, 100 and 500 mg/kg) was analyzed on carbon tetrachloride ( CCl4)-induced acute liver injury. Rats pretreated orally with SDGLEtOH(100 and 500 mg/kg) and silymarin (200 mg/kg) for 3 consecutive days prior to the administration of a single dose of 50% CCl4(0.10 ml/100 g of bw, ip) significantly prevented the increases in the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in CCl4-treated rats. Histological analysis also showed that SDGLEtOH(100 and 500 mg/kg) and silymarin reduced the incidence of liver lesions including vacuole formation, neutrophil infiltration and necrosis of hepatocytes induced by CCl4in rats. Moreover, the SDGLEtOH(100 and 500 mg/kg) increased the activities of anti-oxidative enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRd) and decreased malondialdehyde (MDA) level in liver, as compared to those in the CCl4-treated group. Furthermore, SDGLEtOH(100 and 500 mg/kg) and silymarin attenuated the increased levels of tumor necrosis factor-α (TNF-α) in serum and nitric oxide ( NO ) in liver as compared to the CCl4-treated group. The hepatoprotective mechanisms of SDGLEtOHare likely related to inhibition of TNF-α, MDA and NO productions via increasing the activities of antioxidant enzymes (SOD, GPx and GRd). These experimental results suggest that SDGLEtOHcan attenuate CCl4-induced acute liver injury in rats.

Hepatoprotective Effect of Scoparia dulcis on Carbon Tetrachloride Induced Acute Liver Injury in Mice

2010 ◽  
Vol 38 (04) ◽  
pp. 761-775 ◽  
Author(s):  
Jen-Chieh Tsai ◽  
Wen-Huang Peng ◽  
Tai-Hui Chiu ◽  
Shun-Chieh Huang ◽  
Tai-Hung Huang ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
High Performance ◽  
Acute Liver Injury ◽  
Ethanol Extract ◽  
Significant Inhibition ◽  
Hepatoprotective Effect ◽  
Oxidative Enzymes ◽  
Active Constituents ◽  
Scoparia Dulcis

This study aims to investigate the hepatoprotective activity and active constituents of the ethanol extract of Scoparia dulcis (SDE). The hepatoprotective effect of SDE (0.1, 0.5 and 1 g/kg) was evaluated on the carbon tetrachloride ( CCl4 )-induced acute liver injury. The active constituents were detected by high performance liquid chromatography (HPLC). Mice pretreated orally with SDE (0.5 and 1.0 g/kg) and silymarin (200 mg/kg) for five consecutive days before the administering of a single dose of 0.2% CCl4 (10 ml/kg of bw, ip) showed a significant inhibition of the increase of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Histological analyses also showed that SDE (0.5 and 1.0 g/kg) and silymarin reduced the extent of liver lesions induced by CCl4 , including vacuole formation, neutrophil infiltration and necrosis. Moreover, SDE decreased the malondialdehyde (MDA) level and elevated the content of reduced glutathione (GSH) in the liver as compared to those in the CCl4 group. Furthermore, SDE (0.5 and 1.0 g/kg) enhanced the activities of anti-oxidative enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRd) and glutathione-S-transferase (GST). The quantities of active constituents in SDE were about 3.1 mg luteolin/g extract and 1.1 mg apigenin/g extract. The hepatoprotective mechanisms of SDE were likely associated to the decrease in MDA level and increase in GSH level by increasing the activities of antioxidant enzymes such as SOD, GPx, GRd and GST. These results demonstrated that SDE could alleviate CCl4 -induced acute liver injury in mice.

scholarly journals Hepatoprotective effect of the ethanol extract of Polygonum orientale on carbon tetrachloride-induced acute liver injury in mice

2018 ◽  
Vol 26 (1) ◽  
pp. 369-379 ◽  
Author(s):  
Yung-Jia Chiu ◽  
Shen-Chieh Chou ◽  
Chuan-Sung Chiu ◽  
Chun-Pin Kao ◽  
Kun-Chang Wu ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Ethanol Extract ◽  
Hepatoprotective Effect

scholarly journals Hepatoprotective Effects of Heracleum candicans Against Carbon Tetrachloride-Induced Acute Liver Injury in Rats

Dose-Response ◽  
2021 ◽  
Vol 19 (3) ◽  
pp. 155932582110295
Author(s):  
Jie Li ◽  
Dan Song ◽  
Bintao Zhang ◽  
Jinwei Guo ◽  
Wenping Li ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Neutrophil Infiltration ◽  
Antioxidant Enzyme Activity ◽  
Necrosis Factor Alpha ◽  
Heracleum Candicans ◽  
The Face ◽  
Factor Alpha ◽  
Necrosis Factor

Purpose: To determine the hepatoprotective mechanisms of Heracleum candicans in rats with acute liver injury induced by carbon tetrachloride (CCl4). Methods: Rats were intragastrically administered H candicans twice a day for 14 consecutive days and were intraperitoneally challenged with CCl4. Alanine aminotransferase and aspartate aminotransferase were measured to indicate liver injury. Malondialdehyde antioxidant enzyme activity and tumor necrosis factor-alpha and interleukin 6 secretion were measured as liver injury indicators. Histopathological tests were conducted to determine whether H candicans ameliorated liver injury. Results: CCl4-induced liver injury led to significant increases in liver injury biochemical indicators transaminase and malondialdehyde activities. H candicans pretreatments inhibited these increases. Pathological sections in pretreated samples exhibited reduced vacuole formation, neutrophil infiltration, and necrosis. Conclusion: H candicans increases the antioxidant capacity of the liver and maintains hepatocyte function in the face of CCl4-induced injury.

scholarly journals Protective Effect of Eckol against Acute Hepatic Injury Induced by Carbon Tetrachloride in Mice

Marine Drugs ◽  
2018 ◽  
Vol 16 (9) ◽  
pp. 300 ◽  
Author(s):  
Shulan Li ◽  
Juan Liu ◽  
Mengya Zhang ◽  
Yuan Chen ◽  
Tianxing Zhu ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Protective Effect ◽  
Acute Liver Injury ◽  
Treated Group ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Enhanced Expression ◽  
Pre Treatment

Several in vitro studies have shown the potential hepatoprotective properties of eckol, a natural phlorotannin derived from the brown alga. However, the in vivo hepatoprotective potential of eckol has not been determined. In this study, we performed an in vivo study to investigate the protective effect of eckol and its possible mechanisms on the carbon tetrachloride (CCl4)-induced acute liver injury model in mice. Results revealed that eckol pre-treatment at the dose of 0.5 and 1.0 mg/kg/day for 7 days significantly suppressed the CCl4-induced increases of alanine transaminase (ALT) and aspartate aminotransferase (AST) levels in serum and meliorated morphological liver injury. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) analysis showed that the number of positive apoptotic hepatocytes in the eckol-treated group was lower than that in the CCl4 model group. Western blotting analysis also demonstrated the enhanced expression of bcl-2 and suppressed expression of cleaved caspase-3 by eckol. The CCl4-induced oxidative stress in liver was significantly ameliorated by eckol, which was characterized by reduced malondialdehyde (MDA) formations, and enhanced superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and glutathione (GSH) content. Moreover, the CCl4-induced elevations of pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 were markedly suppressed in the eckol-treated group. However, eckol enhanced the level of IL-10, a potent anti-inflammatory cytokine, and recruited CD11c+ dendritic cells into the liver tissues of CCl4-treated mice. These results indicated that eckol has the protective effect on CCl4-induced acute liver injury via multiple mechanisms including anti-apoptosis, anti-oxidation, anti-inflammation and immune regulation.

Ameliorative Effect ofSilene apricaon Liver Injuries Induced by Carbon Tetrachloride and Acetaminophen

2002 ◽  
Vol 30 (02n03) ◽  
pp. 235-243 ◽  
Author(s):  
Yu-Jen Ko ◽  
Wen-Tsuan Hsieh ◽  
Yueh-Wern Wu ◽  
Wen-Chuan Lin
Keyword(s):  
Lipid Peroxidation ◽  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Oral Administration ◽  
Acute Liver Injury ◽  
Ethanol Extract ◽  
Liver Injuries ◽  
Ameliorative Effect

The effect of oral administration of a 50% ethanol extract of Silene aprica (SA) on acute liver injury was examined in rats intoxicated with carbon tetrachloride (CCl4) and acetaminophen. The results indicated that SA protected the liver from CCl4- and acetaminophen-induced injury as judged by morphological and biochemical observations. An increase in both lipid peroxidation (LPO) and triglyceride concentrations occurred in the liver with CCl4injection , SA administration significantly reduced these changes.

Probiotic and hepatoprotective activity of lactobacillus isolated from Mongolian camel milk products

2019 ◽  
Vol 10 (6) ◽  
pp. 699-710 ◽  
Author(s):  
R.H. Xu ◽  
L. Xiu ◽  
Y.L. Zhang ◽  
R.P. Du ◽  
X. Wang
Keyword(s):  
Liver Injury ◽  
Acute Liver Injury ◽  
Hepatoprotective Activity ◽  
Vital Role ◽  
Camel Milk ◽  
Probiotic Properties ◽  
Pathological Changes ◽  
Milk Products ◽  
Adhesion Ability ◽  
Tnf Α

The improving-intestinal-microbial-balance properties of lactic acid bacteria (LAB) are well known. Thus, LAB could play a vital role in the pathogenesis of liver diseases. In the present study, 107 LAB strains were isolated from Mongolian camel milk products and identified to species, then screened for their probiotic properties. As a result, we identified 71 Lactobacillus bacteria belonging to 9 different species, and 36 Lactococcus bacteria belonging to 8 different species. Among them, six strains of LAB with strong tolerance and adhesion ability were further studied for their protective effect on acute liver injury induced by lipopolysaccharide (LPS)/D-galactosamine (D-GalN). These six strains of LAB were fed to mice for 7 weeks, and on the final day of the experiment, LPS/D-GalN were used to induce acute liver injury. After challenging, the degree of liver pathological changes, secretion of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum and liver, and the expression of tumour necrosis factor (TNF)-α and interleukin (IL)-6 in the liver and intestines were observed and quantified. The results showed that the degree of liver pathological changes in mice fed with the six LAB strains were relieved to varying degrees compared with the LPS/D-GalN-induced model group, and the expressions of AST, ALT, IL-6, and TNF-α factor were also significantly decreased. Moreover, the expression levels of these factors in mice pretreated with Lactobacillus paracasei subsp. paracasei WXD5 were significantly decreased compared with other experimental groups. This suggests the probiotic potential and pharmacological value of L. paracasei subsp. paracasei as a liver injury inhibitor in the intervention of inflammation-based liver disease.

scholarly journals EVALUATION OF HEPATOPROTECTIVE EFFECT OF ETHANOLIC EXTRACT OF ROOT OF PUNICA GRANATUM IN RATS

2017 ◽  
Vol 10 (7) ◽  
pp. 159
Author(s):  
Bushra Hasan Khan ◽  
Farida Ahmad ◽  
Jameel Ahmad ◽  
Syed Mobashir Yunus
Keyword(s):  
Liver Injury ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Hepatoprotective Effect ◽  
Total Serum ◽  
Physical Parameters ◽  
Standard Drug

Objective: To evaluate the hepatoprotective effect of ethanolic extract of the root (REE) of Punica granatum.Methods: This study was conducted on adult albino Wistar rats of either sex weighing 150-200 g. Animals were divided into five groups (n=5). Liver injury was produced by carbon tetrachloride (CCl4) 1 ml/kg dissolved in olive oil (1:1) given intraperitoneally on day 1 and day 4 of the study duration of 14 days. Silymarin (50 mg/kg/d) orally was used as standard drug. Test groups received an REE of P. granatum (REE) at doses of 200 and 400 mg/kg/day orally along with CCl4. On the 15th day, all animals were sacrificed, and blood was collected. Liver was sent for histopathological examination. The hepatoprotective effect of REE was evaluated by assessment of physical parameters, histopathological examination and biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total serum bilirubin.Results: The administration of REE of P. granatum at doses of 200 and 400 mg/kg/day orally, exhibited a highly significant decrease in the rise of mean serum AST, ALT, ALP, and total bilirubin as compared to CCl4 treated group (p<0.001). Histopathological examination of the liver also suggested hepatoprotective effect of REE of P. granatum by restoration of hepatic architecture toward normal. Decrease in the extent of centrilobular necrosis was observed in REE (200 and 400 mg/kg/day) treated rats when compared to CCl4 treated group.Conclusion: This study demonstrated hepatoprotective activity of REE of P. granatum against CCl4 induced liver injury in rats.

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