scholarly journals A novel fluorogenic probe for visualizing the hydrogen peroxide in Parkinson’s disease models

2020 ◽  
Vol 13 (03) ◽  
pp. 2050013
Author(s):  
Gaobin Zhang ◽  
Zheng Li ◽  
Fangjie Chen ◽  
Duoteng Zhang ◽  
Wenhui Ji ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Parkinson’S Disease ◽  
Hydrogen Peroxide ◽  
Parkinson's Disease ◽  
Living Cells ◽  
Emission Peak ◽  
Disease Models ◽  
Fluorogenic Probe ◽  
Highly Sensitive ◽  
Living Organisms

Parkinson’s disease (PD) is closely related to the oxidative stress induced by excess hydrogen peroxide (H2O2) in organisms. Developing an efficient method for noninvasive and real-time H2O2 detection is beneficial to investigate the role played by H2O2 in PD. In this work, a novel fluorogenic probe (CBH) for living organisms H2O2 detection has been designed, synthesized and characterized. The emission of CBH in PBS solution is very weak. However, when H2O2 was added, the fluorescence of CBH solution was sharply increased for 12-fold, accompanied by the emission peak blue-shifted from 600 to 530 nm. Moreover, the response of CBH to H2O2 is highly sensitive and selective and is not affected by various ROS/RNS, anions, cations, and amino acids. Based on the good performance of CBH for H2O2 detection, it has been successfully applied to visualizing the H2O2 concentration in living cells, Zebrafish and [Formula: see text]. elegans PD models.

scholarly journals One-pot synthesis of a hydrogen peroxide-selective fluorogenic probe and its application in Parkinson's disease in vitro and vivo models

2020 ◽  
Vol 1 (5) ◽  
pp. 1448-1454 ◽  
Author(s):  
Yao Lu ◽  
Bo Peng ◽  
Xinghan Qiu ◽  
Xin Li ◽  
Zheng Li ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Hydrogen Peroxide ◽  
Parkinson's Disease ◽  
Multicomponent Reaction ◽  
Living Cells ◽  
Phosphate Ester ◽  
One Pot ◽  
Fluorogenic Probe ◽  
Abnormal Level

An abnormal level of hydrogen peroxide in neurons is strongly implicated in the progression of Parkinson’s disease. A one-pot multicomponent reaction has been utilized to synthesise phosphate ester-based probe for imaging of H2O2 in living cells and Drosophila PD model.

A novel pyrimidine based deep-red fluorogenic probe for detecting hydrogen peroxide in Parkinson's disease models

Talanta ◽  
2019 ◽  
Vol 199 ◽  
pp. 628-633 ◽  
Author(s):  
Xinghan Qiu ◽  
Chenqi Xin ◽  
Wenjing Qin ◽  
Zheng Li ◽  
Duoteng Zhang ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Hydrogen Peroxide ◽  
Parkinson's Disease ◽  
Disease Models ◽  
Fluorogenic Probe

scholarly journals Neuroprotective Effects of Lindleyin on Hydrogen Peroxide-Induced Cell Injury and MPTP-Induced Parkinson’s Disease in C57BL/6 Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Jin-Jie Zhang ◽  
Xiao-Rong Shi ◽  
Wen-Wen Lv ◽  
Xiao-Long Zhou ◽  
Ying-Dong Sun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Parkinson’S Disease ◽  
Hydrogen Peroxide ◽  
Parkinson's Disease ◽  
Cell Injury ◽  
In Vivo Studies ◽  
Antioxidant Enzyme Activities ◽  
Apoptotic Pathway ◽  
Neuroprotective Effects

Oxidative stress (OS) is a crucial factor influencing the development of Parkinson’s disease (PD). Here we first reported that Lindleyin (Lin), one of the major components of rhubarb, possessed neuroprotective effects against H2O2-induced SH-SY5Y cell injury and MPTP-induced PD of C57BL/6 mice. The results showed that Lin can decrease cell death and apoptotic rate induced by H2O2 through inhibiting mitochondrial apoptotic pathway and increasing the activities of SOD, GSH-Px, and CAT as well as decreasing the level of MDA. In addition, in vivo studies showed that oral administration of Lin (5 or 20 mg/kg) showed significant change in motor function deficits, antioxidant enzyme activities, apoptotic pathway, and tyrosine hydroxylase expression. Our results reveal that Lin might be a promising anti-PD agent by reducing OS and apoptosis.

scholarly journals NOX2-Derived Hydrogen Peroxide Impedes the AMPK/Akt-mTOR Signaling Pathway in Parkinson's Disease in Vitro Models

2021 ◽  
Author(s):  
Ruijie Zhang ◽  
Nana Zhang ◽  
Xiaoqing Dong ◽  
Xin Chen ◽  
Jing Ma ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Parkinson’S Disease ◽  
Hydrogen Peroxide ◽  
Parkinson's Disease ◽  
Signaling Pathway ◽  
Neuronal Apoptosis ◽  
Mtor Signaling ◽  
Regulatory Proteins ◽  
Mtor Signaling Pathway ◽  
Compound C

Abstract Oxidative stress is closely related to the pathogenesis of Parkinson's disease (PD), a typical neurodegenerative disease. NADPH oxidase 2 (NOX2) is involved in hydrogen peroxide (H2O2) generation. Recently, we have reported that H2O2 and PD toxins, including 6-hydroxydopamine (6-OHDA), 1-Methyl-4-phenylpyridin-1-ium (MPP+) and rotenone, induce neuronal apoptosis by inhibiting mTOR pathway. Here, we show that 6-OHDA, MPP+ or rotenone induced H2O2 generation by upregulation of NOX2 and its regulatory proteins (p22phox, p40phox, p47phox, p67phox, and Rac1), leading to apoptotic cell death in PC12 cells and primary neurons. Pretreatment with catalase, a H2O2-scavenging enzyme, significantly blocked PD toxins-evoked NOX2-derived H2O2, thereby hindering activation of AMPK, inhibition of Akt/mTOR, induction of apoptosis in neuronal cells. Similar events were also seen in the cells pretreated with Mito-TEMPO, a mitochondria-specific superoxide scavenger, implying a mitochondrial H2O2-dependent mechanism involved. Further research revealed that inhibiting NOX2 with apocynin or silencing NOX2 attenuated the effects of PD toxins on AMPK/Akt/mTOR and apoptosis in the cells. Of importance, ectopic expression of constitutively active Akt or dominant negative AMPKα, or inhibition of AMPK with compound C suppressed PD toxins-induced expression of NOX2 and its regulatory proteins, as well as consequential H2O2 and apoptosis in the cells. Taken together, these results indicate that certain PD toxins can impede the AMPK/Akt-mTOR signaling pathway leading to neuronal apoptosis by eliciting NOX2-derived H2O2. Our findings suggest that neuronal loss in PD may be prevented by regulating of NOX2, AMPK/Akt-mTOR signaling and/or administering antioxidants to ameliorate oxidative stress.

A mitochondria-targeted two-photon fluorogenic probe for the dual-imaging of viscosity and H2O2 levels in Parkinson's disease models

2019 ◽  
Vol 7 (27) ◽  
pp. 4243-4251 ◽  
Author(s):  
Hao Li ◽  
Chenqi Xin ◽  
Gaobin Zhang ◽  
Xisi Han ◽  
Wenjing Qin ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Disease Models ◽  
Two Photon ◽  
Fluorogenic Probe ◽  
Dual Imaging

Novel two-photon fluorogenic probe could simultaneously monitor changes in the mitochondrial viscosity and H2O2 levels using two different channels.

Deep-red fluorogenic probe for rapid detection of nitric oxide in Parkinson’s disease models

2019 ◽  
Vol 283 ◽  
pp. 769-775 ◽  
Author(s):  
Mingrui Weng ◽  
Xue Yang ◽  
Yun Ni ◽  
Chenchen Xu ◽  
Hang Zhang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Rapid Detection ◽  
Disease Models ◽  
Fluorogenic Probe

scholarly journals Antioxidant lipoic acid ligand-shell gold nanoconjugates against oxidative stress caused by α-synuclein aggregates

2020 ◽  
Vol 2 (12) ◽  
pp. 5666-5681
Author(s):  
Maria Elena Piersimoni ◽  
Xiangyu Teng ◽  
Anthony E. G. Cass ◽  
Liming Ying
Keyword(s):  
Oxidative Stress ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Antioxidant System ◽  
Lipoic Acid ◽  
Living Cells ◽  
Acid Ligand ◽  
Ligand Shell

Lipoic acid–gold nanoconjugates (GNPs–LA) decrease GNPs–α-synuclein-induced oxidative stress on living cells, making them a promising antioxidant system for the treatment of Parkinson's disease.

A fluorogenic probe based on chelation–hydrolysis-enhancement mechanism for visualizing Zn2+ in Parkinson's disease models

2019 ◽  
Vol 7 (14) ◽  
pp. 2252-2260 ◽  
Author(s):  
Gaobin Zhang ◽  
Yanfei Zhao ◽  
Bo Peng ◽  
Zheng Li ◽  
Chenchen Xu ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Real Time ◽  
Biological Systems ◽  
Disease Models ◽  
Enhancement Mechanism ◽  
Fluorogenic Probe ◽  
Real Time Detection ◽  
Role Of Zn

Developing efficient methods for real-time detection of Zn2+ level in biological systems is highly relevant to improve our understanding of the role of Zn2+ in the progression of Parkinson's disease (PD).

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