Depletion and refilling of intracellular Ca2+ stores induce oscillations of Ca2+ current

1993 ◽  
Vol 264 (4) ◽  
pp. H1319-H1322 ◽  
Author(s):  
L. Vaca ◽  
D. L. Kunze

An agonist-induced Ca2+ influx pathway in vascular endothelium and other nonexcitable cells is closely aligned with the depletion of microsomal Ca2+ stores. The mechanism by which this occurs is unknown. In these studies 2',5',-di(tert-butyl-1,4-benzohydroquinone, a specific inhibitor of the microsomal Ca(2+)-adenosinetriphosphatase, and patch-clamp recordings were used to evaluate the relationship of inward Ca2+ current to the depletion of intracellular Ca2+ stores. The results demonstrate that depletion and refilling of Ca2+ stores control the amplitude of an electrogenic influx pathway in vascular endothelium. Prominent fluctuations in Ca2+ current occur when there is an imbalance between depletion and refilling of the stores. Furthermore, the studies suggest that the Ca2+ influx pathway is spatially in close association with the intracellular store.

Author(s):  
Gilang Primanagita Rachman ◽  
Indah Riyantini ◽  
Zahidah Hasan ◽  
Herman Hamdani

Seagrass beds in Ujung Genteng waters are one of the shallow marine ecosystems that have relatively high biodiversity. Seagrass ecosystem as one of the coastal ecosystems in these waters has a diversity of marine life that is associated with it. The purpose of this study was to establish the association pattern and the correlation of mega zoobenthos to seagrass ecosystem conditions. The method used was a survey method by conducting a survey direct observation of the stations that have been determined.  Three seagrass species were identified, namely: Thallasia hemprichii, Cymodoceae rotundata and Enhalus acoroides. The highest density of seagrass was found in station 2 with the type of seagrass Thallasia hemprichii as much as 207 ind/m2. Fifteen mega zoobenthos species were identified representing 3 phyla. Species Mega zoobenthos which is most commonly found in Ujung Genteng waters is Ophiothrix fragilis, Ophiocoma erinaceus, and Diadema setosum. The mega zoobenthos Diversity Index at the observation station is included in the medium category that is 2.33-2.66 and the evenness index is 0.63-0.84. Correlation of seagrass density with mega zoobenthos obtained by 0.92 shows that there is a very strong relationship between seagrass density with mega zoobenthos. The result shows that there are 4 patterns of close association and 2 patterns of very close association. Ophiocoma erinaceus of the ophiuroidea class has a pattern of very close positive associations with seagrass Cymodoceae rotundata and Dendrodoris sp of the order nudibranchia has a pattern of very close negative associations with seagrass Cymodoceae rotundata.


1963 ◽  
Vol 11 (2) ◽  
pp. 141 ◽  
Author(s):  
DG Parbery ◽  
RFN Langdon

The present study and a survey of literature showed that of the various spore types associated with species of Phyllachora, scolecospores typical of the form genus Leptostromella were most common. Examination has shown that certain spore types claimed as imperfect states of Phyllachora species are actually spores of hyperparasites. Evidence that the scolecospores belong to the Phyllachora species with which they are associated has been gathered. Studies on the development of P. parilis showed that the scolecospores associated with the perithecia of this species were genetically related to it. Attempts to germinate the scolecospores associated with three species of Phyllachora were unsuccessful. Similarly, when scolecospores 'were used as inoculum, test plants did not become infected. When ascosporic inoculum were used, however, infection occurred. Only one type of mycelium was found in each infection court, and pycnidia and perithecia developed from this in close association. Consequently, although the scolecosporous pycnidia developed prior to the perithecia, both types of fructification occurred in individual Phyllachora colonies. Within each mature colony the tissues of the pycnidia, perithecia, and clypeus became fused and were indistinguishable from one another. The development of the pycnidium and scolecospores is described, and it is shown that a different species of Leptostromella is associated with each different Phyllachora species. The Leptostromella species can be distinguished especially by the morphology of their sporophores. When it was shown that the morphology of the Leptostromella associated with each Phyllachora species was distinctive and constant, it was possible to assess the frequency and distribution of these associations. With some species of Phyllachora the association occurred in all specimens, while in others it was not as frequent. Always, however, the distribution of the association was as widespread as the species of Phyllachora concerned. It was noted that hyperparasites were able to parasitize the Leptostromella states as readily as the ascal states of various species of Phyllachora. The function of the scolecospores is not known, but it is suggested that they may be spermatia.


1966 ◽  
Vol 31 (2) ◽  
pp. 257-278 ◽  
Author(s):  
Jack C. Vaughn

Cytochemical, radioautographic, and microspectrophotometric studies bearing on the relationship of histone transition to the origin and development of the protein and RNA components of the "sphère chromatophile" in the developing spermatogenic cells of the albino rat are presented. These studies show that the sphère chromatophile has many features in common with somatic nuclei: it contains histonelike basic proteins rich in lysine, with lesser amounts of arginine. No evidence is found for the presence of a protamine in this granule. The sphère chromatophile is rich in RNA, but contains no DNA. The failure of a positive reaction with basic protein stains, unless the RNA is first removed, indicates either a chemical bonding or a very close association between the RNA and basic protein. The basic protein and RNA components of the sphère chromatophile appear to have different origins in the cell. A sequence of stages in the development of the lysine-rich basic protein component of this structure commences with the appearance of tiny grains in those spermatid nuclei which are beginning to replace lysine-rich histones with arginine-rich histones. Subsequently, similar-staining cytoplasmic grains appear, which coalesce to form the sphère chromatophile in the cytoplasm. Labeling studies show that the basic protein component is synthesized at about the time of the last premeiotic DNA (and histone) synthesis. The results of the microspectrophotometric measurements support the idea that the basic protein lost from the spermatid nucleus is the source of the basic protein in the sphère chromatophile.


1958 ◽  
Vol s3-99 (47) ◽  
pp. 295-314
Author(s):  
B. L. REID

Living material was photographed by phase-contrast miocroscopy. Fixed material was stained for mitochondria or post-osmicated. The findings show a close conformity between the pictures in living and fixed cells, except that the so-called Golgi apparatus, whilst very apparent in some zones by both techniques, in other zones is well represented after osmication but not in the living cell. Reasons for this are proposed. Studies by earlier workers on the mitochondrial pattern are confirmed. There is an increased density and decreased length of rods down the length of the epididymalduct. The relationship of the chondriome to the osmiophil material varies in the different zones; in some, close association with permeation is found, while in others the separation is complete. General observations upon living epididymal cells are recorded. These include the effects of different suspending media and the changes undergone by the cells after their isolation. A close study has been made of the free (stereociliated) border of the cells.


2006 ◽  
Vol 120 (5) ◽  
pp. 371-374 ◽  
Author(s):  
D W Aird ◽  
P Puttasiddaiah ◽  
S Berry ◽  
C Spyridakou ◽  
M Kumar

Objective: The aim of this study was to identify the distribution of parotid tumours within the gland in relation to the facial nerve branches. Documentation revealing such a relationship has not been reported previously.Method: A prospective study involving 111 patients was carried out over a period of 18 years in a specialist otolaryngology unit within a district general hospital. The relationship of the facial nerve to the tumour was graded into six types. The grading system was then employed to categorize each case.Results: More than two-thirds of the tumours were pleomorphic adenomas. The majority of these were located in the body and not in the tail of the parotid gland. In 50 per cent of these cases, the tumour was in close association with the two major divisions of the facial nerve. In the case of Warthin's tumours, 47 per cent were located in the tail, below the lower division of the facial nerve.


1926 ◽  
Vol 30 (191) ◽  
pp. 668-675
Author(s):  
H. E. Wimperis

Aeronautics is a youthful study—a mere Cinderella of the Sciences, distinguished from her sisters by her tender years, by her guileless and impetuous disregard of the pinch of the financial shoe, and by her beauty.That it is Applied Physics is to me the most inspiring definition of engineering; and if this be true for engineering in general, as I think it is, especially true is it of aeronautics. To illustrate the close association of these new studies with the work of physicists, I shall select a few of the more striking results of recent research.Aviation is now entering upon a new and intensely interesting phase—one which will call for every scientific resource at our command. The materials of -construction are changing : wood is giving place to metal. The engine proves ito have most unexpected possibilities ahead of it through the increase of intake pressure; whilst the very lifting structure itself promises to change, for some purposes at least, from linear motion to rotary.


1966 ◽  
Vol 12 (3) ◽  
pp. 227-231
Author(s):  
William T. Adams ◽  
Richard A. Grant ◽  
Charles S. Prigmore

Correction has a history of inclusion of many academic dis ciplines, many points of view in its application of tasks, and many functions which frequently have been approached from narrow perspectives. A great need has developed to establish joint action among the interest groups. This paper suggests a tentative formulation for a cooperative approach to correctional rehabili tation through the vocational rehabilitation model. The current emphasis on the employment and job training of the offender allows modern means of treatment to be based on such a model. A close association between correctional and vocational rehabili tation programs is proposed.


Paleobiology ◽  
1980 ◽  
Vol 6 (02) ◽  
pp. 146-160 ◽  
Author(s):  
William A. Oliver

The Mesozoic-Cenozoic coral Order Scleractinia has been suggested to have originated or evolved (1) by direct descent from the Paleozoic Order Rugosa or (2) by the development of a skeleton in members of one of the anemone groups that probably have existed throughout Phanerozoic time. In spite of much work on the subject, advocates of the direct descent hypothesis have failed to find convincing evidence of this relationship. Critical points are:(1) Rugosan septal insertion is serial; Scleractinian insertion is cyclic; no intermediate stages have been demonstrated. Apparent intermediates are Scleractinia having bilateral cyclic insertion or teratological Rugosa.(2) There is convincing evidence that the skeletons of many Rugosa were calcitic and none are known to be or to have been aragonitic. In contrast, the skeletons of all living Scleractinia are aragonitic and there is evidence that fossil Scleractinia were aragonitic also. The mineralogic difference is almost certainly due to intrinsic biologic factors.(3) No early Triassic corals of either group are known. This fact is not compelling (by itself) but is important in connection with points 1 and 2, because, given direct descent, both changes took place during this only stage in the history of the two groups in which there are no known corals.


Author(s):  
D. F. Blake ◽  
L. F. Allard ◽  
D. R. Peacor

Echinodermata is a phylum of marine invertebrates which has been extant since Cambrian time (c.a. 500 m.y. before the present). Modern examples of echinoderms include sea urchins, sea stars, and sea lilies (crinoids). The endoskeletons of echinoderms are composed of plates or ossicles (Fig. 1) which are with few exceptions, porous, single crystals of high-magnesian calcite. Despite their single crystal nature, fracture surfaces do not exhibit the near-perfect {10.4} cleavage characteristic of inorganic calcite. This paradoxical mix of biogenic and inorganic features has prompted much recent work on echinoderm skeletal crystallography. Furthermore, fossil echinoderm hard parts comprise a volumetrically significant portion of some marine limestones sequences. The ultrastructural and microchemical characterization of modern skeletal material should lend insight into: 1). The nature of the biogenic processes involved, for example, the relationship of Mg heterogeneity to morphological and structural features in modern echinoderm material, and 2). The nature of the diagenetic changes undergone by their ancient, fossilized counterparts. In this study, high resolution TEM (HRTEM), high voltage TEM (HVTEM), and STEM microanalysis are used to characterize tha ultrastructural and microchemical composition of skeletal elements of the modern crinoid Neocrinus blakei.


Author(s):  
Leon Dmochowski

Electron microscopy has proved to be an invaluable discipline in studies on the relationship of viruses to the origin of leukemia, sarcoma, and other types of tumors in animals and man. The successful cell-free transmission of leukemia and sarcoma in mice, rats, hamsters, and cats, interpreted as due to a virus or viruses, was proved to be due to a virus on the basis of electron microscope studies. These studies demonstrated that all the types of neoplasia in animals of the species examined are produced by a virus of certain characteristic morphological properties similar, if not identical, in the mode of development in all types of neoplasia in animals, as shown in Fig. 1.


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