Enhanced sarcoplasmic reticulum Ca2+ release following intermittent sprint training

2000 ◽  
Vol 279 (1) ◽  
pp. R152-R160 ◽  
Author(s):  
Niels Ørtenblad ◽  
Per K. Lunde ◽  
Klaus Levin ◽  
Jesper L. Andersen ◽  
Preben K. Pedersen
Keyword(s):  
Sarcoplasmic Reticulum ◽  
High Intensity ◽  
Vastus Lateralis ◽  
Ryanodine Receptors ◽  
Vastus Lateralis Muscle ◽  
Sprint Training ◽  
High Intensity Training ◽  
Before And After ◽  
Muscle Biopsies ◽  
Intensity Training

To evaluate the effect of intermittent sprint training on sarcoplasmic reticulum (SR) function, nine young men performed a 5 wk high-intensity intermittent bicycle training, and six served as controls. SR function was evaluated from resting vastus lateralis muscle biopsies, before and after the training period. Intermittent sprint performance (ten 8-s all-out periods alternating with 32-s recovery) was enhanced 12% ( P < 0.01) after training. The 5-wk sprint training induced a significantly higher ( P < 0.05) peak rate of AgNO3-stimulated Ca2+ release from 709 (range 560–877; before) to 774 (596–977) arbitrary units Ca2+ ⋅ g protein− 1 ⋅ min− 1(after). The relative SR density of functional ryanodine receptors (RyR) remained unchanged after training; there was, however, a 48% ( P < 0.05) increase in total number of RyR. No significant differences in Ca2+ uptake rate and Ca2+-ATPase capacity were observed following the training, despite that the relative density of Ca2+-ATPase isoforms SERCA1 and SERCA2 had increased 41% and 55%, respectively ( P < 0.05). These data suggest that high-intensity training induces an enhanced peak SR Ca2+ release, due to an enhanced total volume of SR, whereas SR Ca2+ sequestration function is not altered.

Cross-adaptive responses to different forms of leg training: skeletal muscle biochemistry and histochemistry

1982 ◽  
Vol 60 (5) ◽  
pp. 628-633 ◽  
Author(s):  
W. D. Daub ◽  
H. J. Green ◽  
M. E. Houston ◽  
J. A. Thomson ◽  
I. G. Fraser ◽  
...  
Keyword(s):  
High Intensity ◽  
Vastus Lateralis ◽  
Ice Hockey ◽  
Vastus Lateralis Muscle ◽  
Adaptive Responses ◽  
High Intensity Training ◽  
Muscle Biochemistry ◽  
Slow Twitch ◽  
Fast Twitch ◽  
Intensity Training

The influence of a program of high intensity training and of a combined program of high intensity training and prolonged submaximal training on adaptations to the vastus lateralis muscle was investigated in two groups of elite athletes. The high intensity training (HI) consisted of ice hockey practices and games over a 14-week period while the combined program (HI–LO) included the addition of supplementary sessions of cycling, three times per week, progressively increasing from 30 to 45 min per session and at an intensity of 70% [Formula: see text]. Determinations of enzyme activities representative of energy supplying pathways revealed no change in 3-hydroxyacl CoA dehydrogenase (HADH), total phosphorylase (PHOSP), phosphofructokinase (PFK), lactate dehydrogenase (LDH), and a 7% increase (p < 0.05) in succinate dehydrogenase (SDH). The addition of the supplementary program caused no further adaptation in the metabolic profile. Similarly, neither the HI nor the HI–LO program induced any alteration in the percentage fibre type (slow twitch (ST) vs. fast twitch (FT)) or the subtypes (FTa, FTb, FTc). Reductions in the size (p < 0.05) of ST fibres were noted for both the HI and the HI–LO training programs. In contrast, increases in capillarization (p < 0.05) were found for both the ST (23%) and FTa (32%) fibres for the HI–LO program whereas a reduction in capillarization (21%) occurred in the FTa fibres as a result of HI training only. It is concluded that metabolic differentiation does not appear to occur in a manner consistent with the conditions of energy expenditure at least for high intensity work.

scholarly journals Quantitative determination of Ca2+-dependent Mg2+-ATPase from sarcoplasmic reticulum in muscle biopsies

1989 ◽  
Vol 260 (2) ◽  
pp. 443-448 ◽  
Author(s):  
M E Everts ◽  
J P Andersen ◽  
T Clausen ◽  
O Hansen
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Soleus Muscle ◽  
Human Subjects ◽  
Vastus Lateralis ◽  
Total Concentration ◽  
Vastus Lateralis Muscle ◽  
Lateralis Muscle ◽  
32P Incorporation ◽  
Muscle Biopsies

The possibility of quantifying the total concentration of Ca2+-dependent Mg2+-ATPase of sarcoplasmic reticulum was investigated by measurement of the Ca2+-dependent steady-state phosphorylation from [gamma-32P]ATP and the Ca2+-dependent 3-O-methylfluorescein phosphatase (3-O-MFPase) activity in crude muscle homogenates. The Ca2+-dependent phosphorylation at 0 degree C (mean +/- S.E.) was 40.0 +/- 2.5 (n = 6) and 6.2 +/- 0.7 (n = 4) nmol/g wet wt. in rat extensor digitorum longus (EDL) and soleus muscle, respectively (P less than 0.001). The Ca2+-dependent 3-O-MFPase activity at 37 degrees C was 1424 +/- 238 (n = 6) and 335 +/- 56 (n = 4) nmol/min per g wet wt. in rat EDL and soleus muscle, respectively (P less than 0.01). The molecular activity calculated from these measurements amounted to 35 +/- 5 min-1 (n = 6) and 55 +/- 10 min-1 (n = 4) for EDL and soleus muscle respectively. These values were not different from the molecular activity calculated for purified Ca2+-ATPase (36 min-1). The Ca2+-dependent 32P incorporation in soleus muscle decreased in the order mice greater than rats greater than guinea pigs. In EDL muscles from hypothyroid rats at a 30% reduction of the Ca2+-dependent phosphorylation was observed. The Ca2+-dependent phosphorylation in vastus lateralis muscle from three human subjects amounted to 4.5 +/- 0.8 nmol/g wet wt. It is concluded that measurement of the Ca2+-dependent phosphorylation allows rapid and reproducible quantification of the concentration of Ca2+-dependent Mg2+-ATPase of sarcoplasmic reticulum. Since only 20-60 mg of tissue is required for the measurements, the method can also be used for biopsies obtained in clinical studies.

Hypertension is associated with blunted NO-mediated leg vasodilator responsiveness that is reversed by high-intensity training in postmenopausal women

2020 ◽  
Vol 319 (6) ◽  
pp. R712-R723
Author(s):  
Thomas P. Gunnarsson ◽  
Thomas S. Ehlers ◽  
Thomas Baasch-Skytte ◽  
Anders P. Lund ◽  
Andrea Tamariz-Ellemann ◽  
...  
Keyword(s):  
Postmenopausal Women ◽  
High Intensity ◽  
Disease Risk ◽  
Vascular Dysfunction ◽  
Cardiovascular Disease Risk ◽  
High Intensity Training ◽  
Disease Risk Score ◽  
Before And After ◽  
Markers Of Oxidative Stress ◽  
Intensity Training

The menopausal transition is associated with increased prevalence of hypertension, and in time, postmenopausal women (PMW) will exhibit a cardiovascular disease risk score similar to male counterparts. Hypertension is associated with vascular dysfunction, but whether hypertensive (HYP) PMW have blunted nitric oxide (NO)-mediated leg vasodilator responsiveness and whether this is reversible by high-intensity training (HIT) is unknown. To address these questions, we examined the leg vascular conductance (LVC) in response to femoral infusion of acetylcholine (ACh) and sodium nitroprusside (SNP) and skeletal muscle markers of oxidative stress and NO bioavailability before and after HIT in PMW [12.9 ± 6.0 (means ± SD) years since last menstrual cycle]. We hypothesized that ACh- and SNP-induced LVC responsiveness was reduced in hypertensive compared with normotensive (NORM) PMW and that 10 wk of HIT would reverse the blunted LVC response and decrease blood pressure (BP). Nine hypertensive (HYP (clinical systolic/diastolic BP, 149 ± 11/91 ± 83 mmHg) and eight normotensive (NORM (122 ± 13/75 ± 8 mmHg) PMW completed 10 wk of biweekly small-sided floorball training (4–5 × 3–5 min interspersed by 1–3-min rest periods). Before training, the SNP-induced change in LVC was lower ( P < 0.05) in HYP compared with in NORM. With training, the ACh- and SNP-induced change in LVC at maximal infusion rates, i.e., 100 and 6 µg·min−1·kg leg mass−1, respectively, improved ( P < 0.05) in HYP only. Furthermore, training decreased ( P < 0.05) clinical systolic/diastolic BP (−15 ± 11/−9 ± 7 mmHg) in HYP and systolic BP (−10 ± 9 mmHg) in NORM. Thus, the SNP-mediated LVC responsiveness was blunted in HYP PMW and reversed by a period of HIT that was associated with a marked decrease in clinical BP.

scholarly journals Effects of high-intensity training on muscle lactate transporters and postexercise recovery of muscle lactate and hydrogen ions in women

2008 ◽  
Vol 295 (6) ◽  
pp. R1991-R1998 ◽  
Author(s):  
David Bishop ◽  
Johann Edge ◽  
Claire Thomas ◽  
Jacques Mercier
Keyword(s):  
Relative Abundance ◽  
High Intensity ◽  
Vastus Lateralis ◽  
Interval Training ◽  
Hydrogen Ions ◽  
Muscle Lactate ◽  
High Intensity Training ◽  
Before And After ◽  
High Intensity Interval

The purpose of this study was to investigate the effects of high-intensity interval training (3 days/wk for 5 wk), provoking large changes in muscle lactate and pH, on changes in intracellular buffer capacity (βmin vitro), monocarboxylate transporters (MCTs), and the decrease in muscle lactate and hydrogen ions (H+) after exercise in women. Before and after training, biopsies of the vastus lateralis were obtained at rest and immediately after and 60 s after 45 s of exercise at 190% of maximal O2 uptake. Muscle samples were analyzed for ATP, phosphocreatine (PCr), lactate, and H+; MCT1 and MCT4 relative abundance and βmin vitro were also determined in resting muscle only. Training provoked a large decrease in postexercise muscle pH (pH 6.81). After training, there was a significant decrease in βmin vitro (−11%) and no significant change in relative abundance of MCT1 (96 ± 12%) or MCT4 (120 ± 21%). During the 60-s recovery after exercise, training was associated with no change in the decrease in muscle lactate, a significantly smaller decrease in muscle H+, and increased PCr resynthesis. These results suggest that increases in βmin vitro and MCT relative abundance are not linked to the degree of muscle lactate and H+ accumulation during training. Furthermore, training that is very intense may actually lead to decreases in βmin vitro. The smaller postexercise decrease in muscle H+ after training is a further novel finding and suggests that training that results in a decrease in H+ accumulation and an increase in PCr resynthesis can actually reduce the decrease in muscle H+ during the recovery from supramaximal exercise.

Effect of Q10 Supplementation on Tissue Q10 Levels and Adenine Nucleotide Catabolism During High-Intensity Exercise

1999 ◽  
Vol 9 (2) ◽  
pp. 166-180 ◽  
Author(s):  
Michael Svensson ◽  
Christer Malm ◽  
Michail Tonkonogi ◽  
Bjǒrn Ekblom ◽  
Bertil Sjödin ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
High Intensity ◽  
Adenine Nucleotide ◽  
Recovery Period ◽  
Test Session ◽  
High Intensity Training ◽  
Skeletal Muscle Mitochondria ◽  
Before And After ◽  
Ubiquinone 10 ◽  
Intensity Training

The aim of the present study was to investigate the concentration of ubiquinone-10 (Q10), at rest, in human skeletal muscle and blood plasma before and after a period of high-intensity training with or without Q10 supplementation. Another aim was to explore whether adenine nucleotide catabolism, lipid peroxidation, and mitochondrial function were affected by Q10 treatment. Seventeen young healthy men were assigned to either a control (placebo) or a Q10-supplementation (120 mg/day) group. Q10 supplementation resulted in a significantly higher plasma Q10/lotal cholesterol level on Days 11 and20compared with Day 1. There was no significant change in the concentration of Q10 in skeletal muscle or in isolated skeletal muscle mitochondria in either group. Plasma hypoxanthine and uric acid concentrations increased markedly after each exercise test session in both groups. After the training period, the postexercise increase in plasma hypoxanthine was markedly reduced in both groups, but the response was partially reversed after the recovery period. It was concluded that Q10 supplementation increases the concentration of Q1O in plasma but not in skeletal muscle.

scholarly journals Mechanism of the Effect of High-Intensity Training on Urinary Metabolism in Female Water Polo Players Based on UHPLC-MS Non-Targeted Metabolomics Technique

2021 ◽  
Author(s):  
Leilei Wang ◽  
Anping Chen ◽  
Jianying Li ◽  
Zhuo Sun ◽  
Shiliang Yan ◽  
...  
Keyword(s):  
High Intensity ◽  
Metabolic Pathways ◽  
Shanxi Province ◽  
Training Period ◽  
Formal Training ◽  
Water Polo ◽  
High Intensity Training ◽  
Before And After ◽  
Competition Period ◽  
Intensity Training

Objective: To study the changes in urine metabolism in female water polo players before and after high-intensity training by using ultra-high performance liquid chromatography-mass spectrometry, and to explore the biometabolic characteristics of urine after training and competition. Methods: Twelve young female water polo players (except goalkeepers) from Shanxi Province were selected. A 4-week formal training was started after one week of acclimatization according to experimental requirements. Urine samples (5 ml) were collected before formal training, early morning after 4 weeks of training, and immediately after 4 weeks of training matches, and labeled as T1, T2, and T3, respectively. The samples were tested by LC-MS after pre-treatment. XCMS, SIMCA-P 14.1, and SPSS16.0 were used to process the data and identify differential metabolites. Results: On comparing the immediate post-competition period with the pre-training period (T3 vs T1), 24 differential metabolites involved in 16 metabolic pathways were identified, among which niacin and niacinamide metabolism and purine metabolism were potential post-competition urinary metabolic pathways in the untrained state of the athletes. On comparing the immediate post-competition period with the post-training period (T3 vs T2), 10 metabolites involved in 3 metabolic pathways were identified, among which niacin and niacinamide metabolism was a potential target urinary metabolic pathway for the athletes after training. Niacinamide, 1-methylnicotinamide, 2-pyridone, L-Gln, AMP, and Hx were involved in two metabolic pathways before and after the training. Conclusion: Differential changes in urine after water polo games are due changes in the metabolic pathways of niacin and niacinamide.

scholarly journals Changes in mineral content in trainees’ blood and urine due to high-intensity training

2019 ◽  
pp. 29-35
Keyword(s):  
Physical Activity ◽  
High Intensity ◽  
Mineral Content ◽  
Statistical Significance ◽  
High Intensity Training ◽  
Before And After ◽  
Calcium Magnesium ◽  
Intense Training ◽  
Mineral Concentrations ◽  
Intensity Training

High-intensity training is becoming more popular nowadays when people have less time to engage in prolonged physical activity. Expertly led high intensity training is a safe way to achieve desired fitness goals. The aim of the study was to check if there were significant changes in the concentrations of sodium, potassium, calcium, magnesium, zinc, iron and copper in the blood and urine of twelve trainees after a short but intense training. Blood and urine sampling was performed before and after high intensity training where bodyweight exercises and exercises with external load were used. Statistical analysis was performed using paired t-test (2-tailed) with α=0.05 as statistical significance. The results obtained showed that the measured mineral concentrations varied as a result of intense physical activity, but these variations were small and did not have a general trend of increase or decrease of analyzed mineral content. Based on these results, it can be concluded that, from the standpoint of the mineral concentrations loss, short high-intensity training is safe for the trainee’s health.

Amelioration of Decline in Immune Function in Athletes after High-Intensity Training by Bovine Colostrum

2018 ◽  
Vol 17 (2) ◽  
pp. 219-222
Author(s):  
Zhang Haimin
Keyword(s):  
Immune Function ◽  
High Intensity ◽  
Venous Blood ◽  
Bovine Colostrum ◽  
Control Group ◽  
Intensive Training ◽  
High Intensity Training ◽  
Significant Difference ◽  
Before And After ◽  
Intensity Training

Long-term high-intensity training can cause a decline in immune function in athletes. In this study, we have explored whether bovine colostrum could improve the immune function in athletes undergoing intensive training. Thirty professional athletes were randomly divided into control group and bovine colostrum group; both groups received one month of intensive training. The control group received usual colostrum-free diet and the bovine colostrum group was fed bovine colostrum-rich milk every day. The venous blood was collected from both groups before and after the treatment period. The results showed that the lymphocyte population increased significantly in the bovine colostrum group compared to the control group (P < 0.05). There was no significant change in CD4+ and CD8+ in the two groups (P < 0.05), but the CD3+ and CD4+/CD8+ of the bovine colostrum group were significantly higher than those of the control group. There were significant differences in albumin, globulin and albumin/globulin ratio in the control group (P < 0.05), but not in the bovine colostrum group after training. The bovine colostrum group had lower serum albumin and albumin/globulin (P < 0.05) and higher globulin (P < 0.05) compared to the control group. There was no significant difference in immunologic indexes before and after training in both groups (P > 0.05). The decline in the markers of immune function after high-intensity training was ameliorated by the intake of bovine colostrum.

Effects of high-intensity training and acute exercise on in vitro function of rat sarcoplasmic reticulum

2007 ◽  
Vol 99 (6) ◽  
pp. 641-649 ◽  
Author(s):  
Satoshi Matsunaga ◽  
Takashi Yamada ◽  
Takaaki Mishima ◽  
Makoto Sakamoto ◽  
Minako Sugiyama ◽  
...  
Keyword(s):  
Sarcoplasmic Reticulum ◽  
High Intensity ◽  
Acute Exercise ◽  
High Intensity Training ◽  
Intensity Training

scholarly journals Heart Rate Distribution and Aerobic Fitness Changes During Preseason in Elite Soccer Players

Proceedings ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 23
Author(s):  
Vasilios Kalapotharakos ◽  
Dimitrios Serenidis ◽  
Savvas Tokmakidis
Keyword(s):  
Heart Rate ◽  
Aerobic Fitness ◽  
High Intensity ◽  
Soccer Players ◽  
Training Intensity ◽  
High Intensity Training ◽  
Professional Soccer ◽  
Before And After ◽  
Run Test ◽  
Intensity Training

Aim: Soccer is characterized as an intense intermittent team sport. Heart rate (HR) is used to monitor the players’ training response, as well as to quantify microcycle and mesocycle training intensity during preseason and in-season periods. The purpose of the present study was to quantify the preseason training intensity distribution in elite soccer players and then examine the relationship between HR distribution and changes in aerobic fitness. Material & Method: Sixteen elite professional soccer players (age, 26.8 ± 3.8 years; weight, 77.8 ± 7.7 kg; height, 1.79 ± 0.06 m; mean ± SD) participated in the study. Aerobic fitness was evaluated with VO2max, running velocity at VO2max (v-VO2max) during a laboratory incremental aerobic test and with the distance completed during an interval shuttle run test (ISRT), before and after preseason. HR of each player was measured using a short-range telemetry HR transmitter strap at 5-s intervals during all training sessions of the preseason. The absolute (min) and relative (%) time spent in high-intensity HR zone (90–100% of HRmax) during the preseason period was calculated for each player. Results: VO2max and distances completed during ISRT improved significantly (p < 0.05) by 3.3 ± 2.1% and 29 ± 16%, respectively. The time (%) players spent in high-intensity training was significantly correlated (p < 0.01) with the changes (%) in distance completed during ISRT. Conclusions: These results provide useful information about the HR quantification during preseason in elite soccer players. Additionally, coaches have to take into consideration the time soccer players spend in high-intensity training for optimal endurance responses when planning and implementing the preseason training period.

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