Effect of hypoxia and CO on a cytochrome P-450-mediated reaction in rabbit lungs

1981 ◽  
Vol 51 (6) ◽  
pp. 1635-1642 ◽  
Author(s):  
A. Knoblauch ◽  
A. Sybert ◽  
N. J. Brennan ◽  
J. T. Sylvester ◽  
G. H. Gurtner
Keyword(s):  
Partial Pressure ◽  
Reaction Rate ◽  
Component Model ◽  
High Affinity ◽  
O2 Partial Pressure ◽  
Tissue Po2 ◽  
Two Component ◽  
Carrier Molecule ◽  
The Relationship ◽  
Cytochrome P 450

Evidence indicates that the diffusion of O2 and CO in tissue may be facilitated by a carrier molecule having a P50 that approximates tissue O2 partial pressure (PO2; 1–15 Torr) and a much higher affinity for CO than for O2. To determine whether cytochrome P-450 in lung satisfies these criteria, we measured the effect of hypoxia and of CO on the rate of metabolism of the cytochrome P-450 mediated O-demethylation of p-nitroanisole in isolated perfused rabbit lungs. Metabolism was inhibited by 50% of a control at an estimated tissue PO2 fo 4 Torr (5.5 microM). When inspired CO2 was kept at 200 Torr and inspired CO partial pressure (PCO) varied an estimated tissue PCO/PO2 ratio of 0.025 reduced the reaction rate by 50% of control, but some metabolism persisted at PCO/PO2 ratios larger than one. The relationship between reaction rate and PCO/PO2 ratio could not be fit by a single value for Haldane constant for M (CO affinity/O2 affinity) but could be described with a two-component model in which metabolism was equally divided between a high-affinity cytochrome (M = 200) and a low-affinity cytochrome (M = 2). These findings suggest that cytochrome P-450 could act as a carrier for O2 and CO in tissue with low PO2's.

Effect of O2 Partial Pressure on Post Annealed Ba2YCU3O7-δ Thin Films

1992 ◽  
Vol 275 ◽  
Author(s):  
Julia M. PhUlips ◽  
M. P. Siegal ◽  
S. Y. Hou ◽  
T. H. Tiefel ◽  
J. H. Marshall
Keyword(s):  
Partial Pressure ◽  
Film Growth ◽  
Epitaxial Films ◽  
Pinning Force ◽  
Low Oxygen ◽  
O2 Partial Pressure ◽  
Growth Method ◽  
Lower Temperature ◽  
The Relationship

ABSTRACTEpitaxial films of Ba2YCu3O7-δ (BYCO) as thin as 250 å A and with Jc's approaching those of the best in situ grown films can be formed by co-evaporating BaF2, Y, and Cu followed by a two-stage anneal. These results extend the work on films > 2000 Å thick by R. Feenstra et al. [J. Appl. Phys. 69, 6569 (1991)]. High quality films of these thicknesses become possible if low oxygen partial pressure [p(O2) = 4.3 Torr] is used during the high temperature portion cf the anneal (Ta). The BYCO melt line is the upper limit for Ta. The use of low p(O2) shifts the window for stable BYCO film growth to lower temperature, which allows the formation of smooth films with greater microstructural disorder than is found in films grown in p(O2) = 740 Torr at higher Ta. The best films annealed in p(O2)=4.3 Torr have Jc values a factor of four higher than do comparable films annealed in P2=740 Torr. The relationship between the T required to grow films with the strongest pinning force and p(O2) is log independent of growth method (in situ or situ) over a range of five orders of magnitude of P(O2).

scholarly journals Sodium pump-mediated ATP:ADP exchange. The sided effects of sodium and potassium ions.

1982 ◽  
Vol 80 (6) ◽  
pp. 915-937 ◽  
Author(s):  
J H Kaplan
Keyword(s):  
Exchange Reaction ◽  
Reaction Rate ◽  
Sodium Pump ◽  
Potassium Ions ◽  
Red Cell ◽  
Maximal Inhibition ◽  
High Affinity ◽  
Biphasic Effect ◽  
The Relationship ◽  
Sodium And Potassium

Resealed human red cell ghosts containing caged ATP (Kaplan et al., 1978) and [3H]ADP were irradiated at 340 nm. The photochemical release of free ATP initiated a rapid transphosphorylation reaction (ATP:ADP exchange), a component of which is inhibited by ouabain. The reaction rate was measured by following the rate of appearance of [3H]ATP. The sodium pump-mediated ATP:ADP exchange reaction showed high-affinity stimulation by Mg ions (less than 10 microM) and was inhibited at higher levels. At optimal [Mg], extracellular Na (Nao) had a biphasic effect. Nao progressively inhibited the reaction rate between 0 and 10 mM and stimulated at higher levels. Intracellular Na (Nai) activated the reaction; the rate was maximal when Nai was 1 mM and remained unaltered up to 115 mM Nai at constant Nao. Extracellular K ions (Ko) inhibited the reaction; at high Nao, half-maximal inhibition was observed with 0.9 mM Ko. Lio inhibited the exchange rate with a lower affinity than Ko; half-maximal inhibition was produced by approximately 50 mM Lio. Intracellular K ions were without dramatic effect on the reaction rate in the concentration range where Ko inhibited completely. The relationship between these observations and previous studies on porous preparations is discussed, as well as the extent to which these observations support the hypothesis that the sodium pump-mediated ATP:ADP exchange reaction accompanies the Na:Na exchange transport mode of the sodium pump.

scholarly journals High NH3-SCR reaction rate with low dependence on O2 partial pressure over Al-rich Cu–*BEA zeolite

RSC Advances ◽  
2021 ◽  
Vol 11 (17) ◽  
pp. 10381-10384
Author(s):  
Yusuke Ohata ◽  
Takeshi Ohnishi ◽  
Takahiko Moteki ◽  
Masaru Ogura
Keyword(s):  
Partial Pressure ◽  
Reaction Rate ◽  
Reaction Condition ◽  
Bea Zeolite ◽  
Nh3 Scr ◽  
High Reaction ◽  
O2 Partial Pressure ◽  
Zeolite P ◽  
High Reaction Rate

It was revealed that Al-rich Cu–*BEA zeolite exhibit high reaction rate for NH3-SCR at 473 K in low PO2 reaction condition.

Temporal Experience: A Two Component Model

1988 ◽  
Vol 66 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Charles G. Frederickson
Keyword(s):  
Regression Analysis ◽  
Multiple Regression Analysis ◽  
Multiple Regression ◽  
Significant Relationship ◽  
Component Model ◽  
Temporal Orientation ◽  
Temporal Experience ◽  
Experience Of Time ◽  
Two Component ◽  
The Relationship

The experience of time, or temporal experience, has been postulated to be derived from an interrelation of two components, succession and duration. These components are represented in the literature as temporal orientation and temporal pace, respectively. This postulate of interrelatedness was tested by examining the relationship between measures of temporal orientation and pace assessed simultaneously and coincidentally during 102 psychotherapeutic sessions. Multiple regression analysis indicated a statistically significant relationship between these variables. The nature of their relation and the implications for considering both aspects of temporal experience in research are discussed.

Cytochrome P 450-linked monooxygenase: involvement in the lamb ductus arteriosus

1984 ◽  
Vol 246 (4) ◽  
pp. H640-H643 ◽  
Author(s):  
F. Coceani ◽  
N. C. Hamilton ◽  
J. Labuc ◽  
P. M. Olley
Keyword(s):  
Cytochrome P450 ◽  
Guinea Pig ◽  
Partial Pressure ◽  
Contractile Response ◽  
Muscle Tone ◽  
Ductus Arteriosus ◽  
Inhibitory Effect ◽  
Gas Mixture ◽  
O2 Partial Pressure ◽  
Cytochrome P 450

CO and metyrapone were tested on isolated ductus arteriosus preparations from mature fetal lambs equilibrated at low (4-12 Torr) and high (511-712 Torr) O2 partial pressure (PO2). CO completely reversed the contractile tension of the ductus at either PO2 value and was equally effective in the absence and presence of indomethacin. CO relaxation still occurred when the tissue was exposed to a gas mixture having a CO-to-O2 ratio of 0.27. The inhibitory effect of CO on the O2-contracted ductus was variably reversed by light. Metyrapone was also a relaxant agent, and its action was greater at low than at high PO2. Unlike the lamb ductus, the O2-constricted guinea pig ductus did not respond to CO (maximum CO-to-O2 ratio around 1), whereas it was relaxed by metyrapone. These findings implicate a cytochrome P450-catalyzed enzymic process in the maintenance of muscle tone of the lamb ductus arteriosus and in the contractile response of the vessel to O2.

Radiometric Chronology of Neh–nar Glacier, Kashmir

1982 ◽  
Vol 28 (98) ◽  
pp. 91-105 ◽  
Author(s):  
V. N. Nijampurkar ◽  
N. Bhandari ◽  
C. P. Vohra ◽  
V. Krishnan
Keyword(s):  
Average Rate ◽  
Component Model ◽  
Vertical Profiles ◽  
Kashmir Valley ◽  
Movement Rate ◽  
Accumulation Zone ◽  
Core Samples ◽  
Surface Samples ◽  
Two Component

AbstractSurface and core samples of Neh–nar Glacier in the Kashmir Valley have been analysed for the radionuclides 32Si. 210Pb, 40K, and 137Cs. The lateral and vertical profiles (at an altitude of about 4 140 m) reveal:(1)32Si activity decreasing slowly from the accumulation zone to 4 050 m altitude and then abruptly towards the snout.(2)Five zones of alternating high and low 210Pb activity in the surface samples.(3)An horizon at between 2 and 3 m depth containing 210Pb activity above natural levels. This horizon is also associated with 137Cs and a maximum in total ß activity.The ice samples have been dated on the basis of a simplified two–component model, the “fresh“contribution determined by 2l0Pb and the old component by 32Si. The following conclusions can be drawn from these observations:(1)The model age of the snout ice is c. 850 years.(2)The average rate of ice movement in the lower glacier is about 2 m/year, which compares well with the annual movement rate of 2.65 m/year observed since 1974.

scholarly journals Estimation of Galactic Model Parameters by Interval Method

1996 ◽  
Vol 169 ◽  
pp. 713-714
Author(s):  
S. A. Kutuzov
Keyword(s):  
Mutual Interaction ◽  
Component Model ◽  
Model Parameters ◽  
Interval Method ◽  
Two Component ◽  
The Galaxy ◽  
Galactic Model

The interval method of estimating model parameters (MPs) for the Galaxy was suggested earlier (Kutuzov 1988). Intervals are proposed to be used both for observational estimates of galactic parameters (GPs) and for the values of MPs. In this work we consider a model as a tool for studying mutual interaction of GPs. Two-component model is considered (Kutuzov, Ossipkov 1989). We have to estimate the array P of eight MPs.

scholarly journals Are rate and selectivity correlated in iridium-catalysed hydrogen isotope exchange reactions?

2021 ◽  
Author(s):  
Daria S. Timofeeva ◽  
David M Lindsay ◽  
W. J. Kerr ◽  
David James Nelson
Keyword(s):  
Functional Groups ◽  
Hydrogen Isotope ◽  
Isotope Exchange ◽  
Reaction Rate ◽  
Exchange Reactions ◽  
Reaction Selectivity ◽  
Hydrogen Isotope Exchange ◽  
The Relationship

Herein we examine the relationship between reaction rate and reaction selectivity in iridium-catalysed hydrogen isotope exchange (HIE) reactions directed by Lewis basic functional groups. We have recently develped a directing...

Separation of carotid body chemoreceptor responses to O2 and CO2 by oligomycin and by antimycin A

1982 ◽  
Vol 242 (3) ◽  
pp. C200-C206 ◽  
Author(s):  
E. Mulligan ◽  
S. Lahiri
Keyword(s):  
Steady State ◽  
Carbonic Anhydrase ◽  
Partial Pressure ◽  
Carotid Body ◽  
Body Tissue ◽  
Antimycin A ◽  
Tissue Ph ◽  
Co2 Partial Pressure ◽  
O2 Partial Pressure ◽  
Metabolic Hypothesis

The cat carotid chemoreceptor O2 and CO2 responses can be separated by oligomycin and by antimycin A. Both of these agents greatly diminish or abolish the chemoreceptor O2 response but not the nicotine or CO2 responses. After either oligomycin or antimycin, the responses to increases and decreases in arterial CO2 partial pressure (PaCO2) consisted of increases and decreases in activity characterized respectively by exaggerated overshoots and undershoots. These were eliminated by the carbonic anhydrase inhibitor, acetazolamide, suggesting that they resulted from changes in carotid body tissue pH. The steady-state PaCO2 response remaining after oligomycin was no longer dependent on arterial O2 partial pressure (PaO2). All effects of antimycin were readily reversible in about 20 min. The separation of the responses to O2 and CO2 indicates that there may be at least partially separate pathways of chemoreception for these two stimuli. The similarity of the oligomycin and antimycin results supports the metabolic hypothesis of chemoreception.

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