Fatigue from incompletely fused tetanic contractions in skeletal muscle in situ

1983 ◽  
Vol 55 (3) ◽  
pp. 976-982 ◽  
Author(s):  
B. R. MacIntosh ◽  
W. N. Stainsby ◽  
L. B. Gladden
Keyword(s):  
Skeletal Muscle ◽  
Contractile Response ◽  
Muscle Group ◽  
Energy Availability ◽  
Tetanic Contraction ◽  
Plantaris Muscle ◽  
Pentobarbital Sodium ◽  
Peak Tension ◽  
The Difference

The purpose of this study was to investigate the contractile response of skeletal muscle in situ when stimulation results in an unfused tetanic contraction. The left gastrocnemius-plantaris muscle group of anesthetized (pentobarbital sodium) dogs (n = 16) was connected to an isometric lever and stimulated indirectly for 30 min. During 10-Hz stimulation, total tension (the peak of each oscillation in tension) increased during the first 2 min of stimulation (staircase), then decreased during the remaining 28 min of stimulation. Since relaxation was incomplete at this rate of stimulation, the developed tension, the difference between peak tension and the lowest tension between successive contractions, did not follow the same pattern of staircase and fatigue as the peak tension did. Developed tension (delta T) decreased during the staircase response then increased from 2 to 10 min before finally decreasing again during the last 20 min, ending at 56 +/- 15 (mean +/- SE) % of the initial (first contraction) delta T. At 2 min of 10-Hz contractions, half-relaxation time (1/2 RT) was too long to measure (insufficient relaxation between contractions), but later, 1/2 RT decreased from greater than 65 ms to less than 40 ms. Increased 1/2 RT has been associated with reduced energy availability. If an increased 1/2 RT is an indication of insufficient energy, then it can be concluded that fatigue continued in spite of a recovery of energy supplies. This suggests a possible dissociation of fatigue and energy availability.

Interaction of hyperoxia and blood flow during fatigue of canine skeletal muscle in situ

1979 ◽  
Vol 47 (5) ◽  
pp. 1018-1024 ◽  
Author(s):  
J. K. Barclay ◽  
C. M. Boulianne ◽  
B. A. Wilson ◽  
S. J. Tiffin
Keyword(s):  
Skeletal Muscle ◽  
Blood Flow ◽  
Muscle Blood Flow ◽  
Experimental Period ◽  
Muscle Stimulation ◽  
Plantaris Muscle ◽  
Pentobarbital Sodium ◽  
The Right

Right and left gastrocnemius-plantaris muscle preparations in 20 dogs anesthetized with pentobarbital sodium were used to investigate the effect of hyperoxia on tension maintenance. Muscles were stimulated via the sciatic nerve for 20 min at 60 200-ms tetanic contractions/min (10 impulses/contraction). Direct muscle stimulation after the experimental period resulted in no significant change in tension. In control experiments the tension developed by the right or left muscles over the 20 min was not different. The tension developed by muscles perfused with hyperoxic blood decreased 14% after 20 min, whereas tension in the normoxic muscles decreased 35%. Blood flow in the hyperoxic muscles was significantly higher at 20 min (P less than 0.05). Pump perfusion of one of a pair of normoxic muscles resulted in a tension decrease of 13% in the pump-perfused muscles, whereas tension in the control muscles decreased 34%. Tension maintenance was flow dependent. The effect of hyperoxia could be mediated through the involvement of oxygen in the long-term control of muscle blood flow.

Length-dependent activation of in situ canine skeletal muscle

1979 ◽  
Vol 237 (1) ◽  
pp. C38-C42 ◽  
Author(s):  
C. R. Lambert ◽  
L. B. Gladden ◽  
W. N. Stainsby
Keyword(s):  
Skeletal Muscle ◽  
Muscle Length ◽  
Muscle Group ◽  
Plantaris Muscle ◽  
Tension Development ◽  
Inotropic State ◽  
Isometric Twitch ◽  
Length Dependent Activation ◽  
Different Levels

This study was designed to assess the contribution of length-dependent activation to the peak isometric twitch tension developed and the maximal rate of tension development (dP/dt) of in situ canine skeletal muscle. Length-developed tension and length-dP/dt relationships were generated for the dog gastrocnemius-plantaris muscle group at three different levels of inotropic state as determined by stimulation frequency. These relationships were then normalized with respect to maximal developed tension and maximal dP/dt and the normalized curves were superimposed for comparison. At progressively shorter muscle lengths the augmentation of tension production by a given increment in inotropic state was greater as measured by either developed tension or dP/dt. Thus, a given change in muscle length produced a greater change in performance in less potentiated muscles. These findings are similar to those from studies of isolated cardiac muscle and illustrate the lack of independence between activational state and muscle length for in situ skeletal muscle.

Plasma catecholamines and their effect on blood lactate and muscle lactate output

1984 ◽  
Vol 57 (2) ◽  
pp. 321-325 ◽  
Author(s):  
W. N. Stainsby ◽  
C. Sumners ◽  
G. M. Andrew
Keyword(s):  
Venous Blood ◽  
Plasma Catecholamines ◽  
Muscle Group ◽  
O2 Uptake ◽  
Plantaris Muscle ◽  
Muscle Lactate ◽  
Blood Samples ◽  
Lactate Output ◽  
Before And After

This study was designed to test the hypothesis that epinephrine (E) and norepinephrine (NE) increase net muscle lactate output (L) of in situ gastrocnemius-plantaris muscle group during contractions. Plasma [E] and [NE] were measured before and after the surgical isolation of the muscle and at 10-min intervals during the 60-min experiments. Plasma [E] and [NE] were increased threefold by intravenous infusions of E (n = 3) or NE (n = 3) at a rate of 1.5 micrograms X kg body wt-1 X min-1. Arterial and muscle venous blood samples for O2 and lactate concentrations were also obtained. The infusions began at min 11 and repetitive isometric contractions (4 tw/s) began at min 31. The presurgery plasma [E] and [NE] averaged 0.34 and 0.52 ng/ml, respectively, and rose to 1.12 and 1.19 ng/ml 10 min after surgery. Arterial and venous lactate concentrations (CaL and CvL) increased continuously during E infusion but remained constant during NE infusion. Maximal L during the first 10 min of contractions was significantly increased compared with an identical earlier study without infusions. O2 uptake was not changed by the infusions. It is concluded that E causes CaL to rise and that both E and NE increase maximal net lactate output during contractions.

Functional and metabolic consequences of skeletal muscle remodeling in hypothyroidism

1991 ◽  
Vol 260 (2) ◽  
pp. E272-E279 ◽  
Author(s):  
R. M. McAllister ◽  
R. W. Ogilvie ◽  
R. L. Terjung
Keyword(s):  
Skeletal Muscle ◽  
Citrate Synthase ◽  
Control Group ◽  
Type I ◽  
Tetanic Contraction ◽  
Plantaris Muscle ◽  
Tension Development ◽  
Fiber Area ◽  
And Control ◽  
Muscle Remodeling

Functional and metabolic responses of hypothyroid skeletal muscle were evaluated during steady-state isometric contraction conditions, using an isolated perfused rat hindlimb preparation. Treating rats with propylthiouracil (PTU) for 4-5 mo resulted in a 55% decrease (P less than 0.001) in citrate synthase activity in plantaris muscle and phenotypic remodeling of the plantaris, evident by a threefold increase in type I fiber area and a 13% decrease in type II fiber area. Perfusion of PTU (n = 9) and control (n = 9) rat hindlimbs of similar size, with similar inflow (approximately 10 ml/min) and oxygen content (approximately 20 g/100 ml), resulted in similar oxygen deliveries to the contracting muscles (PTU 11.4 +/- 0.58, control 9.54 +/- 0.75 mumol.min-1.g-1; P greater than 0.05). Ten-minute tetanic contraction (100 ms at 100 Hz) periods at 4, 8, 15, 30, and 45 tetani/min were elicited in consecutive ascending order. Oxygen consumption (VO2) was lower in the PTU group at all contraction frequencies (P less than 0.005), with a decrease in peak VO2 of 44% (PTU 3.01 +/- 0.29, control 5.35 +/- 0.42 mumol.min-1.g-1; P less than 0.001). Oxygen extraction by the PTU muscle was only approximately 25% of that delivered. Developed tension was initially less (15%; P less than 0.05) in the PTU group but declined in a similar manner, as a percent of initial, to that of the control group. The slightly lower absolute tension development of the PTU muscle could not account for the large reduction in VO2.(ABSTRACT TRUNCATED AT 250 WORDS)

Nerve stimulation decreases malonyl-CoA in skeletal muscle

1992 ◽  
Vol 72 (3) ◽  
pp. 901-904 ◽  
Author(s):  
C. Duan ◽  
W. W. Winder
Keyword(s):  
Sciatic Nerve ◽  
Creatine Phosphate ◽  
Muscle Group ◽  
Carnitine Palmitoyl Transferase ◽  
Plantaris Muscle ◽  
Malonyl Coa ◽  
Left And Right ◽  
Muscle Groups ◽  
Stimulation Of

This study was designed to determine the effect of in situ electrical stimulation of the sciatic nerve on malonyl-CoA, an inhibitor of carnitine palmitoyl transferase, in the gastrocnemius/plantaris muscle group of rats. The left sciatic nerve was stimulated at a frequency of 5 Hz with 100-ms trains of impulses (50 Hz) for 1, 3, or 5 min. At the end of stimulation, the left and right (nonstimulated) gastrocnemius/plantaris muscle groups were clamp-frozen and later analyzed for malonyl-CoA and other metabolites. No change was observed in the noncontracting contralateral muscles in malonyl-CoA, ATP, creatine phosphate (CP), or citrate. In the stimulated muscles, malonyl-CoA decreased from 1.7 +/- 0.1 to 1.0 +/- 0.1 nmol/g (P less than 0.05), and CP decreased from 15.8 +/- 0.9 to 12.2 +/- 1.0 mumol/g (P less than 0.05) after 3 min of stimulation. After 5 min of stimulation, malonyl-CoA was 1.0 +/- 0.1 nmol/g and CP was 10.3 +/- 1.3 mumol/g. When muscles were stimulated for 5 min with single impulses (5 Hz), malonyl-CoA was decreased from 1.8 +/- 0.3 to 1.0 +/- 0.1 nmol/g, with no change in CP, ATP, or adenosine 3′,5′-cyclic monophosphate. Thus a decline in malonyl-CoA can be induced by muscle contraction independently of humoral influence.

Control of malonyl-CoA by glucose and insulin in perfused skeletal muscle

1993 ◽  
Vol 74 (5) ◽  
pp. 2543-2547 ◽  
Author(s):  
C. Duan ◽  
W. W. Winder
Keyword(s):  
Skeletal Muscle ◽  
Potent Inhibitor ◽  
Carnitine Palmitoyltransferase ◽  
Muscle Group ◽  
Plantaris Muscle ◽  
Bicarbonate Buffer ◽  
Malonyl Coa ◽  
Flow Through ◽  
Carnitine Palmitoyltransferase I ◽  
Isolated Rat

This study was designed to determine the effects of glucose and insulin on malonyl-CoA, the potent inhibitor of carnitine palmitoyltransferase I, in the gastrocnemius/plantaris muscle group. Isolated rat hindlimbs were perfused with Krebs-Henseleit bicarbonate buffer containing fresh erythrocytes (hematocrit = 41) and albumin in a flow-through mode for 60 min. Two experiments were performed. In the first, hindlimbs were perfused with medium containing no glucose and no insulin (n = 9) or with medium containing 10 mM glucose and 100 microU/ml of insulin (n = 9). Gastrocnemius/plantaris malonyl-CoA was 0.6 +/- 0.1 nmol/g in the absence of glucose and insulin vs. 1.4 +/- 0.1 nmol/g when both glucose and insulin were added. In the second experiment, hindlimbs were perfused with medium containing 10 mM glucose alone, 200 microU insulin alone, or with a combination of 10 mM glucose and 200 microU/ml of insulin (n = 8 for each). Malonyl-CoA was decreased in gastrocnemius/plantaris perfused with glucose alone (0.7 +/- 0.2 nmol/g) and with insulin alone (0.7 +/- 0.1 nmol/g) compared with hindlimbs perfused with the combination of glucose and insulin (1.4 +/- 0.2 nmol/g). We conclude that both glucose and insulin are required for preventing a decline in muscle malonyl-CoA.

Contractile properties of old rat muscles: effect of increased use

1989 ◽  
Vol 67 (4) ◽  
pp. 1401-1408 ◽  
Author(s):  
H. Klitgaard ◽  
R. Marc ◽  
A. Brunet ◽  
H. Vandewalle ◽  
H. Monod
Keyword(s):  
Skeletal Muscle ◽  
Strength Training ◽  
Weight Ratio ◽  
Contractile Properties ◽  
Male Rats ◽  
Strongly Correlated ◽  
Plantaris Muscle ◽  
Muscle Weight ◽  
Peak Tension ◽  
Time To Peak

To examine how different kinds of activity affect the composition and contractile properties of aging skeletal muscle, old male rats were strength and swim trained. The mass of weights lifted during the strength training increased by 85 +/- 9% (P less than 0.05), which was accompanied by an increase by 32 +/- 5% (P less than 0.05) of the estimated force developed. The wet muscle weight of the soleus and the plantaris decreased significantly with age. The phenomenon was counteracted but not neutralized by the strength training. Twitch and tetanic tension also decreased significantly with age in both the soleus and plantaris muscle. This was avoided by the strength training. This training also significantly decreased time to peak tension and half-relaxation time of both muscles. The swim training increased the heart-to-body weight ratio by 21 +/- 5% (P less than 0.05) and the endurance of the soleus muscle. Time to peak tension and triosephosphate dehydrogenase activity of the plantaris muscle were strongly correlated (P less than 0.001) with myosin adenosinetriphosphatase activity. The results show that the composition and contractile properties of old skeletal muscle are considerably affected by strength training repeated during a substantial period of old age, whereas swim training only affects the endurance of the skeletal muscle.

O2 uptake and developed tension during and after fatigue, curare block, and ischemia

1978 ◽  
Vol 45 (5) ◽  
pp. 751-755 ◽  
Author(s):  
L. B. Gladden ◽  
B. R. MacIntosh ◽  
W. N. Stainsby
Keyword(s):  
Neuromuscular Block ◽  
Muscle Group ◽  
O2 Uptake ◽  
Plantaris Muscle ◽  
Continuous Stimulation ◽  
Before And After

The purpose of this study was to investigate the effect of changes in developed tension on the ratio between O2 uptake and isometric developed tension in the in situ dog gastrocnemius-plantaris muscle group. O2 uptake and isometric developed tension of the muscle were measured during contractions at 1 twitch/s before and after fatigue with both single and twin impulses (6.5 V, 0.2-ms duration; the twins were separated by 10--20 ms). Twin impulses prior to fatigue raised developed tension to two times the tension developed with single impulses. Fatigue was obtained by stimulation at 10--20 impulses/s for 30--40 min. Twin impulses after fatigue returned developed tension to the level of single impulses before fatigue. O2 uptake and developed tension were also measured during the slower development of fatigue produced by continuous stimulation (3, 4, 5, and 6 impulses/s) as well as during “fatigue” induced by partial neuromuscular block with curare or ischemia. In all cases, there was no change in the O2 uptake per unit of tension developed, indicating a constant coupling between O2 uptake and developed tension.

scholarly journals The combination of smoking with vitamin D deficiency impairs skeletal muscle fiber hypertrophy in response to overload in mice

2021 ◽  
Author(s):  
Ajime Tom Tanjeko ◽  
Jef Serré ◽  
Rob C.I. Wüst ◽  
Jatin G. Burniston ◽  
Karen Maes ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Muscle Fiber ◽  
Internal Control ◽  
Whole Body ◽  
Plantaris Muscle ◽  
Air Exposure ◽  
Hypertrophic Response

Vitamin D deficiency, which is highly prevalent in the general population, exerts similar deleterious effects on skeletal muscles to those induced by cigarette smoking. We examined whether cigarette smoke (CS) exposure and/or vitamin D deficiency impairs the skeletal muscle hypertrophic response to overload. Male C57Bl/6JolaH mice on a normal or vitamin D-deficient diet were exposed to CS or room air for 18 weeks. Six weeks after initiation of CS or air exposure, sham surgery or denervation of the agonists of the left plantaris muscle was performed. The right leg served as internal control. Twelve weeks later, the hypertrophic response was assessed. CS exposure instigated loss of body and muscle mass, and increased lung inflammatory cell infiltration (p<0.05), independently of diet. Maximal exercise capacity, whole body strength, in situ plantaris muscle force and key markers of hypertrophic signaling (Akt, 4EBP1, FoxO1) were not significantly affected by smoking or diet. The increase in plantaris muscle fiber cross-sectional area in response to overload was attenuated in vitamin D-deficient CS-exposed mice (smoking x diet interaction for hypertrophy, p=0.03). In situ fatigue resistance was elevated in hypertrophied plantaris, irrespective of vitamin D deficiency and/or CS exposure. In conclusion, our data show that CS exposure or vitamin D deficiency alone did not attenuate the hypertrophic response of overloaded plantaris muscles, but this hypertrophic response was weakened when both conditions were combined. These data suggest that current smokers who also present with vitamin D deficiency may be less likely to respond to a training program.

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