Transient changes in muscle high-energy phosphates during moderate exercise

1993 ◽  
Vol 75 (2) ◽  
pp. 648-656 ◽  
Author(s):  
G. D. Marsh ◽  
D. H. Paterson ◽  
J. J. Potwarka ◽  
R. T. Thompson
Keyword(s):  
Oxygen Consumption ◽  
Steady State ◽  
Recovery Period ◽  
High Energy ◽  
Second Order ◽  
Moderate Intensity ◽  
High Energy Phosphates ◽  
Order Model ◽  
Time Constants ◽  
First Order

The purpose of this study was to use 31P-nuclear magnetic resonance spectroscopy to examine changes in wrist flexor muscle metabolism during the transitions from rest to steady-state exercise (on-transient) and back to rest (off-transient). Five healthy young males (mean age 25 +/- 2 yr) performed a series of square-wave exercise tests, each consisting of 5 min of moderate-intensity work followed by a 5-min recovery period. The subjects repeated this protocol six times, and each individual's results were pooled before analysis. ATP and intracellular pH did not change significantly during exercise or recovery. Phosphocreatine (PCr) declined progressively at the onset of exercise, reaching a plateau after approximately 2 min. A reciprocal increase in Pi occurred during the onset of exercise. During the recovery period PCr was resynthesized, whereas Pi returned to resting levels. The data were plotted as a function of time and fit with both first- and second-order exponential growth or decay models; however, the second-order model did not significantly improve the fit of the data. Time constants for the first-order model of the on- and off-transient responses for both PCr and Pi were approximately 30 s. These values are nearly identical to the time constants for oxygen consumption during submaximal exercise that have been reported previously by several authors. The results of this study show that the metabolism of muscle PCr during steady-state exercise and recovery can be accurately described by a monoexponential model and, further, suggest that a first-order proportionality exists between metabolic substrate utilization and oxygen consumption.

Letters to the Editor

1998 ◽  
Vol 275 (2) ◽  
pp. H726-H729
Author(s):  
J. A. L. Jeneson ◽  
M. J. Kushmerick ◽  
H. V. Westerhoff
Keyword(s):  
Oxygen Consumption ◽  
Steady State ◽  
Western Blot ◽  
Northern Blot ◽  
Adenine Nucleotide ◽  
Respiratory Control ◽  
High Energy ◽  
High Energy Phosphates ◽  
Adenine Nucleotide Translocator

The following is the abstract of the article discussed in the subsequent letter: Portman, Michael A., Yun Xiao, Ying Song, and Xue-Han Ning. Expression of adenine nucleotide translocator parallels maturation of respiratory control in heart in vivo. Am. J. Physiol. 273 ( Heart Circ. Physiol. 42): H1977–H1983, 1997.—Changes in the relationship between myocardial high-energy phosphates and oxygen consumption in vivo occur during development, implying that the mode of respiratory control undergoes maturation. We hypothesized that these maturational changes in sheep heart are paralleled by alterations in the adenine nucleotide translocator (ANT), which are in turn related to changes in the expression of this gene. Increases in myocardial oxygen consumption (MV˙o 2) were induced by epinephrine infusion in newborn (0–32 h, n = 6) and mature sheep (30–32 days, n = 6), and high-energy phosphates were monitored with 31P nuclear magnetic resonance. Western blot analyses for the ANT1 and the β-subunit of F1-adenosinetriphosphatase (ATPase) were performed in these hearts and additional ( n = 9 total per group) as well as in fetal hearts (130–132 days of gestation, n = 5). Northern blot analyses were performed to assess for changes in steady-state RNA transcripts for these two genes. Kinetic analyses for the31P spectra data revealed that the ADP-MV˙o 2 relationship for the newborns conformed to a Michaelis-Menten model but that the mature data did not conform to first- or second-order kinetic control of respiration through ANT. Maturation from fetal to mature was accompanied by a 2.5-fold increase in ANT protein (by Western blot), with no detectable change in β-F1-ATPase. Northern blot data show that steady-state mRNA levels for ANT and β-F1-ATPase increased ∼2.5-fold from fetal to mature. These data indicate that 1) respiratory control pattern in the newborn is consistent with a kinetic type regulation through ANT, 2) maturational decreases in control through ANT are paralleled by specific increases in ANT content, and 3) regulation of these changes in ANT may be related to increases in steady-state transcript levels for its gene.

Expression of adenine nucleotide translocator parallels maturation of respiratory control in heart in vivo

1997 ◽  
Vol 273 (4) ◽  
pp. H1977-H1983 ◽  
Author(s):  
Michael A. Portman ◽  
Yun Xiao ◽  
Ying Song ◽  
Xue-Han Ning
Keyword(s):  
Oxygen Consumption ◽  
Steady State ◽  
Western Blot ◽  
Northern Blot ◽  
Adenine Nucleotide ◽  
Respiratory Control ◽  
High Energy ◽  
High Energy Phosphates ◽  
Adenine Nucleotide Translocator

Changes in the relationship between myocardial high-energy phosphates and oxygen consumption in vivo occur during development, implying that the mode of respiratory control undergoes maturation. We hypothesized that these maturational changes in sheep heart are paralleled by alterations in the adenine nucleotide translocator (ANT), which are in turn related to changes in the expression of this gene. Increases in myocardial oxygen consumption (MV˙o 2) were induced by epinephrine infusion in newborn (0–32 h, n = 6) and mature sheep (30–32 days, n = 6), and high-energy phosphates were monitored with 31P nuclear magnetic resonance. Western blot analyses for the ANT1 and the β-subunit of F1-adenosinetriphosphatase (ATPase) were performed in these hearts and additional ( n = 9 total per group) as well as in fetal hearts (130–132 days of gestation, n = 5). Northern blot analyses were performed to assess for changes in steady-state RNA transcripts for these two genes. Kinetic analyses for the31P spectra data revealed that the ADP-MV˙o 2 relationship for the newborns conformed to a Michaelis-Menten model but that the mature data did not conform to first- or second-order kinetic control of respiration through ANT. Maturation from fetal to mature was accompanied by a 2.5-fold increase in ANT protein (by Western blot), with no detectable change in β-F1-ATPase. Northern blot data show that steady-state mRNA levels for ANT and β-F1-ATPase increased ∼2.5-fold from fetal to mature. These data indicate that 1) respiratory control pattern in the newborn is consistent with a kinetic type regulation through ANT, 2) maturational decreases in control through ANT are paralleled by specific increases in ANT content, and 3) regulation of these changes in ANT may be related to increases in steady-state transcript levels for its gene.

scholarly journals Peculiarities of Zigzag Behaviour in Linear Models of Ship Yaw Motion

2016 ◽  
Vol 23 (1) ◽  
pp. 23-38
Author(s):  
Jarosław Artyszuk
Keyword(s):  
Linear Models ◽  
Nonlinear Models ◽  
Second Order ◽  
Hydrodynamic Coefficients ◽  
Quality Indices ◽  
Order Model ◽  
Present Survey ◽  
Time Constants ◽  
First Order ◽  
Ship Steering

Abstract The present survey, as part of larger project, is devoted to properties of pure linear models of yaw motion for directionally stable ships, of the first- and second-order, sometimes referred to as the Nomoto models. In rather exhaustive way, it exactly compares and explains both models in that what is being lost in the zigzag behaviour, if the reduction to the simpler, first-order dynamics (K-T model) is attempted with the very famous [Nomoto et al., 1957] approximation: T = T1 + T2 - T3. The latter three time constants of the second-order model, more physically sound, are strictly dependent on the hydrodynamic coefficients of an essential part of the background full-mission manoeuvring model. The approximation of real ship behaviour in either of the mentioned linearity orders, and the corresponding complex parameters may facilitate designing and evaluating ship steering, and identifying some regions of advanced nonlinear models, where linearisation is valid.As a novel outcome of the conducted investigation, a huge inadequacy of such a first- -order model for zigzag simulation is reported. If this procedure is used for determining steering quality indices, those would be of course inadequate, and the process of utilizing them (e.g. autopilot) inefficient.

Reduced substrate oxidation in postischemic myocardium: 13C and 31P NMR analyses

1990 ◽  
Vol 258 (5) ◽  
pp. H1357-H1365 ◽  
Author(s):  
E. D. Lewandowski ◽  
D. L. Johnston
Keyword(s):  
Steady State ◽  
31P Nmr ◽  
Tca Cycle ◽  
High Energy ◽  
Substrate Oxidation ◽  
High Energy Phosphates ◽  
Atp Content ◽  
And Control ◽  
13C And 31P Nmr ◽  
Postischemic Myocardium

13C and 31P nuclear magnetic resonance (NMR) spectra were used to assess substrate oxidation and high-energy phosphates in postischemic (PI) isolated rabbit hearts. Phosphocreatine (PCr) increased in nonischemic controls on switching from glucose perfusion to either 2.5 mM [3-13C]pyruvate (120%, n = 7) or [2-13C]acetate (114%, n = 8, P less than 0.05). ATP content, oxygen consumption (MVO2), and hemodynamics (dP/dt) were not affected by substrate availability in control or PI hearts. dP/dt was 40-60% lower in PI hearts during reperfusion after 10 min ischemia. Hearts reperfused with either pyruvate (n = 11) or acetate (n = 8) regained preischemic PCr levels within 45 s. Steady-state ATP levels were 55-70% of preischemia with pyruvate and 52-60% with acetate. Percent maximum [4-13C]glutamate signal showed reduced conversion of pyruvate to glutamate via the tricarboxylic acid (TCA) cycle at 4-min reperfusion (PI = 24 +/- 4%, means +/- SE; Control = 48 +/- 4%). The increase in 13C signal from the C-4 position of glutamate was similar to control hearts within 10.5 min. The increase in [4-13C]glutamate signal from acetate was not different between PI and control hearts. The ratio of [2-13C]Glu:[4-13C]Glu, reflecting TCA cycle activity, was reduced in PI hearts with acetate for at least 10 min (Control = 0.76 +/- 0.03; PI = 0.51 +/- 0.09) until steady state was reached. Despite rapid recovery of oxidative phosphorylation, contractility remained impaired and substrate oxidation was significantly slowed in postischemic hearts.

scholarly journals Examining the Impostor-Profile—Is There a General Impostor Characteristic?

2021 ◽  
Vol 12 ◽  
Author(s):  
Fabio Ibrahim ◽  
Johann-Christoph Münscher ◽  
Philipp Yorck Herzberg
Keyword(s):  
Convergent Validity ◽  
Factor Model ◽  
Second Order ◽  
Model Fit ◽  
Impostor Phenomenon ◽  
General Factor ◽  
Order Model ◽  
First Order ◽  
Order Factor ◽  
Best Fit

The Impostor-Profile (IPP) is a six-dimensional questionnaire measuring the Impostor Phenomenon facets. This study aims to test (a) the appropriateness of a total score, (b) measurement invariance (MI) between gender, (c) the reliability of the IPP, and (d) the convergent validity of the IPP subscales. The sample consisted of N = 482 individuals (64% female). To identify whether the scales of the IPP form a total score, we compared four models: (1) six correlating subscales, (2) a general factor model, (3) a second-order model with one second-order factor and six first-order factors, and (4) a bifactorial model with six group factors. The bifactorial model obtained the best fit. This supports the assumption of a total impostor score. The inspection of structural validity between gender subgroups showed configural, metric, and partial scalar MI. Factor mean comparisons supported the assumption that females and males differ in latent means of the Impostor Phenomenon expressions. The omega coefficients showed sufficient reliability (≥0.71), except for the subscale Need for Sympathy. Overall, the findings of the bifactor model fit and construct validity support the assumption that the measurement through total expression is meaningful in addition to the theoretically formulated multidimensionality of the Impostor Phenomenon.

Second-Order Correlation of Klinkenberg-Corrected Permeability and its Experimental Verification on Heterogeneously-Stressed Gas Shale

2020 ◽  
Author(s):  
Yufei Chen ◽  
Changbao Jiang ◽  
Juliana Y. Leung ◽  
Andrew K. Wojtanowicz ◽  
Dongming Zhang ◽  
...  
Keyword(s):  
Experimental Data ◽  
Second Order ◽  
Gas Shale ◽  
Order Model ◽  
First Order ◽  
Gas Slippage ◽  
Steady State Method ◽  
Slippage Effect ◽  
Stress Heterogeneity ◽  
Second Order Model

Abstract Shale is an extremely tight and fine-grained sedimentary rock with nanometer-scale pore sizes. The nanopore structure within a shale system contributes not only to the low to ultra-low permeability coefficients (10−18 to 10−22 m2), but also to the significant gas slippage effect. The Klinkenberg equation, a first-order correlation, offers a satisfying solution to describe this particular phenomenon for decades. However, in recent years, several scholars and engineers have found that the linear relation from the Klinkenberg equation is invalid for most gas shale reservoirs, and a need for a second-order model is, therefore, proceeding apace. In this regard, the purpose of this study was to develop a second-order approach with experimental verifications. The study involved a derivation of a second-order correlation of the Klinkenberg-corrected permeability, followed by experimental verifications on a cubic shale sample sourced from the Sichuan Basin in southwestern China. We utilized a newly developed multi-functional true triaxial geophysical (TTG) apparatus to carry out permeability measurements with the steady-state method in the presence of heterogeneous stresses. Also discussed were the effects of two gas slippage factors, Klinkenberg-corrected permeability, and heterogeneous stress. Finally, based on the second-order slip theory, we analyzed the deviation of permeability from Darcy flux. The results showed that the apparent permeability increased more rapidly as the pore pressure declined when the pore pressures are relatively low, which is a strong evidence of the gas slippage effect. The second-order model could reasonably match the experimental data, resulting in a lower Klinkenberg-corrected permeability compared with that from the linear Klinkenberg equation. That is, the second-order approach improves the intrinsic permeability estimation of gas shales with the result being closer to the liquid permeability compared with the Klinkenberg approach. Analysis of the experimental data reported that both the first-order slippage factor A and the second-order slippage factor B increased with increasing stress heterogeneity, and that A was likely to be more sensitive to stress heterogeneity compared with B. Interestingly, both A and B first slightly increased and then significantly as the permeability declined. It is recommended that when the shale permeability is below 10−18 m2, the second-order approach should be taken into account. Darcy’s law starts to deviate when Kn > 0.01 and is invalid at high Knudsen numbers. The second-order approach seems to alleviate the problem of overestimation compared with the Klinkenberg approach and is more accurate in permeability evolution.

Regulation of oxygen consumption in fast- and slow-twitch muscle

1992 ◽  
Vol 263 (3) ◽  
pp. C598-C606 ◽  
Author(s):  
M. J. Kushmerick ◽  
R. A. Meyer ◽  
T. R. Brown
Keyword(s):  
Steady State ◽  
Biceps Brachii ◽  
Ex Vivo ◽  
Recovery Period ◽  
Respiratory Control ◽  
Cellular Respiration ◽  
O2 Consumption ◽  
Half Time ◽  
First Order ◽  
Slow Twitch

Phosphorus nuclear magnetic resonance spectra and steady-state O2 consumption rates were obtained from ex vivo arterially perfused cat biceps brachii (fast twitch) and soleus (slow twitch) muscles during and after periods of isometric twitch stimulation at 30 degrees C. In the biceps muscles, steady-state O2 consumption increased and phosphocreatine (PCr) concentration decreased progressively with stimulation. PCr recovery after these stimulation periods followed first-order kinetics with a half time of 10 min. The results in the biceps could be explained by a feedback control of cellular respiration by ADP concentration. In the soleus, steady-state O2 consumption also increased and PCr concentration decreased as stimulation rates increased. The half time for PCr recovery in the soleus was approximately 5 min, but, in contrast to the pattern in the biceps, the kinetics was not first order. There was an overshoot during the recovery period in the PCr content of soleus and a corresponding undershoot of Pi compared with resting values. Mitochondrial regulation by ADP is not sufficient to account for respiratory control in slow-twitch soleus. The respiration rate in neither muscle was dependent on the Pi content. Thus we conclude that the mechanism of control of cellular respiration is both quantitatively and qualitatively different in fast- and slow-twitch skeletal muscle.

Global energetics of fast magnetic reconnection

1988 ◽  
Vol 40 (3) ◽  
pp. 505-515 ◽  
Author(s):  
M. Jardine ◽  
E. R. Priest
Keyword(s):  
Steady State ◽  
Total Energy ◽  
Second Order ◽  
Weakly Nonlinear ◽  
First Order ◽  
Weakly Nonlinear Theory ◽  
Special Cases ◽  
Pile Up ◽  
Different Types ◽  
Converging Flow

We examine the global energetics of a recent weakly nonlinear theory of fast steady-state reconnection in an incompressible plasma (Jardine & Priest 1988). This is itself an extension to second order of the Priest & Forbes (1986) family of models, of which Petschek-like and Sonnerup-like solutions are special cases. While to first order we find that the energy conversion is insensitive to the type of solution (such as slow compression or flux pile-up), to second order not only does the total energy converted vary but so also does the ratio of the thermal to kinetic energies produced. For a slow compression with a strongly converging flow, the amount of energy converted is greatest and is dominated by the thermal contribution, while for a flux pile-up with a strongly diverging flow, the amount of energy converted is smallest and is dominated by the kinetic contribution. We also find that the total energy flowing out of the downstream region can be increased either by increasing the external magnetic Mach number Me or the external plasma beta βe Increasing Me also enhances the variations between different types of solutions.

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