scholarly journals Synthesis of Amino Acid Analogues of 5H-Dibenz[b,f]azepine and Evaluation of their Radical Scavenging Activity

2009 ◽  
Vol 6 (1) ◽  
pp. 125-132 ◽  
Author(s):  
H. Vijay Kumar ◽  
C. R. Gnanendra ◽  
Nagaraja Naik
Keyword(s):  
Free Radical ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Hydroxy Proline ◽  
Amino Acid Analogues

A method for the synthesis of tyrosine, phenyl alanine, hydroxy proline and threonine free amino acid analogues of 5H-dibenz[b,f]azepine is proposed. 5H-dibenz[b,f]azepine was prepared by known method. The key intermediate 3-chloro-1-(5H-dibenz[b,f]azepine-5-yl)propan-1-one was obtained byN-acylation of 5H-dibenz[b,f]azepine with 3-chloro propionyl chloride. Further coupling of respective free amino acid to produce 2-(3-(5H-dibenz[b,f]azepine-5-yl)-3-oxopropylamino)3-(4 hydroxyphenyl) propanoic acid, 2-(3-(5H-dibenz[b,f]azepine-5-yl)-3-oxopropylamino)-3-phenyl propanoicacid,1-(3-(5H-dibenz[b,f]azepine-5-yl)-3-oxopropyl)-3-hydroxypyrolidine-2-carboxylic acid and 2-(3-(5H-dibenz[b,f] azepine-yl)-3-oxopropyl amino)-3-hydroxy butanoic acid. The synthesized compounds were evaluated for their potential over 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity. Butylated hydroxy anisole (BHA) and ascorbic acid (AA) were used as the reference antioxidant compounds and also the comparative study with synthesized compounds was done. Under our experimental conditions tyrosine, hydroxy proline and threonine analogues possess a direct scavenging effect on trapping the stable free radical DPPH. Hydroxy proline analogues showed a significant radical scavenging activity among the synthesized analogues

scholarly journals Comparative analysis of antioxidative potentials of extracts of defatted unfermented and fermented locust beans

2015 ◽  
Vol 49 (4) ◽  
pp. 275-280
Author(s):  
B Daramola
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidative Capacity ◽  
Locust Bean

Preliminary investigation on antioxidative potentials of defatted fermented locust bean extract in comparison to defatted non-fermented locust bean was conducted. Qualitative antioxidative assessment on the extract was accomplished by screening the phytochemical endowment, and similar bioactive components. Also some antioxidative indices: relative reducing power (mg ascorbic acid reducing activity equivalent per mg sample), radical scavenging activity(%) and free amino acids (mg glycine activity equivalent per mg sample), were quantitatively evaluated. Phytochemical screening results showed that the defatted fermented samples were characterized with high strength presence of bioactive compounds notably phenolic compounds, saponins, peptides, amino acid and reductones. However, alkaloids and flavonoids were not detected. UV-Spectral characteristics of the extracts corroborated same. Similarly, quantitative antioxidative markers evaluated showed that the antioxidative capacity in terms of relative reducing power (23.40-29.50), and radical scavenging activity (82-89) on 1,1-diphenyl-2-picrylhydrazyl (DPPH) of defatted fermented locust bean extracts were high in comparison to the low antioxidative capacity in terms of relative reducing power 10.00-13.30) and radical scavenging activity (53-57) of the corresponding defatted unfermented locust bean extract. In addition the free amino acid of the defatted fermented locust bean extracts were high (1.66-3.30) in comparison to the low (0.215-0.3) free amino acid of the defatted unfermented locust bean extract. The result of total phenolic content was variable. Also, the antioxidant activity rate content, and EC50 of the extract were evaluated. This study demonstrated high antioxidant endowment of fermented locust beans in comparison to non-fermented locust beans. DOI: http://dx.doi.org/10.3329/bjsir.v49i4.22632 Bangladesh J. Sci. Ind. Res. 49(4), 275-280, 2014

Bioactive Composition of Pig Laryngeal Cartilage Extracts and Their Free Radical-scavenging Activity

2006 ◽  
Vol 12 (5) ◽  
pp. 371-377 ◽  
Author(s):  
Shuang-li Xiong ◽  
Zheng-yu Jin ◽  
An-lin Li
Keyword(s):  
Free Radical ◽  
Amino Acid ◽  
Superoxide Anion ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Laryngeal Cartilage ◽  
Hydroxyl Free Radical

This work focused on amino acid and mineral element analysis of pig laryngeal cartilage. Papain and DEAE-Sepharose fast flow ion exchange chromatography were used to hydrolyase cartilage and purify hydrolysate. Further study was performed to compare the free radical-scavenging activity of various fractions. Cartilage is abundant in bioactive amino acids such as glycine, glutamic acid and arginine and minerals such as calcium, magnesium and iron. Four polysaccahrides attached peptides and one peptide were isolated from hydrolysate. Weak 2,2-diphenyl-1-picryhydrazyl free radical scavenging activities of all substances were observed. Other fractions showed hydroxyl free radical and superoxide anion free radical scavenging ability in a concentration-dependent manner except fraction A. The hydroxyl free radical scavenging activity of tested samples decreased in the following order: fraction C crude chondroitin sulphate (ChS) peptide fraction B semi-purified ChS purified ChS. Significant differences were observed between purified ChS and other fractions (P 0.01). For superoxide anion free radical the order was: peptide purified ChS semi-purified ChS crude ChS fraction C fraction B. The peptide was significantly higher than purified ChS (P 0.01), which was significantly higher than the other components (P 0.01). According to the results, polysaccharides, ChS, amino acid and peptide present in cartilage had important bioactivity.

scholarly journals Hypotensive effect of Cichorium intybus extract in rats

2021 ◽  
Vol 10 (2) ◽  
pp. 257-261
Author(s):  
Mehrnoosh Sedighi ◽  
Mostafa Cheraghi ◽  
Mahdieh Faghihi ◽  
Mohammad Rahimi-Madiseh ◽  
Ali Asghar Kiani ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Cichorium Intybus ◽  
Free Radical Scavenging Activity ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Phenolic And Flavonoid

Introduction: Oxidative stress is involved in many diseases, including hypertension, kidney failure, and heart disease. This study aimed to evaluate the effect of hydroalcoholic Cichorium intybus extract on blood pressure in rats. Antioxidant activity, phenolic and flavonoid contents of the plant extract were also evaluated. Methods: In this study, 32 male Wistar rats weighing 250-300 g were divided into four groups of eight each. Animals in the control group were administered with normal saline and in the C. intybus groups with extract at 25, 50, and 100 mg/kg for two weeks. Then, the homodynamic parameters were examined by the Power lab. The phenolic and flavonoid contents were also evaluated by a spectrophotometer and the rate of free radical scavenging activity was measured by the diphenyl-1-picyryl-hydrazyl (DPPH) free radical method. Results: The free radical scavenging activity of C. intybus extract was obtained 47.85% of DPPH, and flavonoid and phenolic contents were 8.21 and 27.19 mg/g of dry extract, respectively. Meanwhile, median (MAP), systolic (SAP) and diastolic arterial pressure (DAP) significantly decreased in the 50 mg/kg extract-treated group compared to the control and 200 mg/kg extract-treated groups. Conclusion: Ethanol extract of C. intybus plays a protective role against hypertension, which, in part, might be due to antioxidant compounds of the plant against free radicals.

scholarly journals Free radical scavenging activity of taro (Colocasia esculenta (L.) Schott) varieties with varying flesh color

2020 ◽  
pp. 23-32
Author(s):  
Erlinda Vasquez ◽  
Precila Contero ◽  
Dilberto Ferraren
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reduction Rate ◽  
Free Radical Scavenging ◽  
Differential Method ◽  
Free Radical Scavenging Activity ◽  
Antioxidant Compounds ◽  
Scavenging Activity

Corms of five taro varieties namelyztwo NSIC-registered varieties, VG-1 (Kalpao) and VG-2 (Iniito), and three farmer’s variety, PRG 322 (Chowking), PRG 381 (Pandaga), and PRG 380 (Salot), were evaluated for antioxidant activity using 1, 1-diphenyl-2 picrylhydrazyl (DPPH) assay before and after cooking. The purple-fleshed varieties PRG 322, PRG 380 and VG-2 showed higher antioxidant activity than theyellow- and cream-fleshed varieties, The monomeric anthocyanin contents of PRG 380 and VG-1 PRG 322 and VG-2, which showed the highest free radical scavenging activity were measured using the pH differential method. The free» radical scavenging activity ofthe five taro varieties ranged from 134 to 154TEμm g-1 when raw and 127 to 147TEμm g-1 when cooked with the highest activity in raw PRG 322 followed by raw PRG 380 and VG-2. Cooked PRG 381 and VG-1 showed the lowest activity, Cooking reduced the activity by 4.5-10.2% with PRG 380 showing the highest reduction rate while the other four varieties did not vary significantly from 4.5-5.9%. Decreased in antioxidant activity can be due to the degradation and leaching out of antioxidant compounds during steamed cooking.

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