scholarly journals Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein fromViscum articulatumand Its Biological Effects on Peripheral Blood Mononuclear Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-9
Author(s):  
Tzu-Li Lu ◽  
Jing-Yuan Chuang ◽  
Jai-Sing Yang ◽  
Shau-Ting Chiu ◽  
Nai-Wan Hsiao ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Biological Effects ◽  
Ribosome Inactivating Protein ◽  
Peripheral Blood Mononuclear ◽  
Ribosome Inactivating Proteins ◽  
Preferential Binding ◽  
A Chain ◽  
Low Ionic Strength

Type-2 ribosome-inactivating proteins, composed of a toxic A-chain and lectin-like B-chain, display various biological functions, including cytotoxicity and immunomodulation. We here cloned the lectin-like B-chain encoding fragment of a newly identified type-2 RIP gene,articulatingene, fromViscum articulatum, into a bacterial expression vector to obtain nonglycosylated recombinant protein expressed in inclusion bodies. After purification and protein refolding, soluble refolded recombinant articulatin B-chain (rATB) showed lectin activity specific toward galactoside moiety and was stably maintained while stored in low ionic strength solution. Despite lacking glycosylation, rATB actively bound leukocytes with preferential binding to monocytes andin vitrostimulated PBMCs to release cytokines without obvious cytotoxicity. These results implicated such a B-chain fragment as a potential immunomodulator.

In vitro production of type 1 and type 2 cytokines by peripheral blood mononuclear cells from high-risk HIV-negative intravenous drug users

AIDS ◽  
1995 ◽  
Vol 9 (7) ◽  
pp. 691-694 ◽  
Author(s):  
Wilma Barcellini ◽  
Gian Paolo Rizzardi ◽  
Claudio Velati ◽  
Maria Orietta Borghi ◽  
Cristina Fain ◽  
...  
Keyword(s):  
Drug Users ◽  
Mononuclear Cells ◽  
In Vitro Production ◽  
Peripheral Blood Mononuclear ◽  
Type 2 Cytokines ◽  
Vitro Production ◽  
Hiv Negative

scholarly journals Effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patients

2006 ◽  
Vol 124 (4) ◽  
pp. 219-222 ◽  
Author(s):  
Maria Cristina Foss-Freitas ◽  
Norma Tiraboschi Foss ◽  
Eduardo Antonio Donadi ◽  
Milton Cesar Foss
Keyword(s):  
Metabolic Control ◽  
Mononuclear Cells ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
In Vitro Proliferation ◽  
Peripheral Blood Mononuclear ◽  
Type 2 Diabetic

CONTEXT AND OBJECTIVE: Diabetes mellitus is a clinical syndrome that frequently leads to the development of chronic complications and high susceptibility to infections. It is probably due to defective immunological defense, which may be related to metabolic control of the disease. The aim of this study was to evaluate the effect of metabolic control on immune-cell behavior in type 1 and type 2 diabetic patients. For this, the in vitro proliferation of peripheral blood mononuclear cells (PBMC) was analyzed in patients with inadequate and adequate metabolic control. DESIGN AND SETTING: Experimental/laboratory study at a university hospital. METHODS: Eleven type 1 and thirteen type 2 diabetic patients were studied, together with 21 healthy individuals divided in two groups (11/10), who were matched by sex and age with those diabetic patients. PBMC cultures stimulated with concanavalin-A (Con-A) were used to measure ³H-thymidine incorporation after 72 hours of cell culturing. For patients with inadequate metabolic control, culturing was performed on the first day of patient hospitalization and again after intensive treatment to achieve adequate control. RESULTS: The proliferation index for Con-A-stimulated cultures from type 1 diabetic patients was significantly greater than that for cultures from healthy individuals and type 2 diabetic patients, independent of metabolic control. A negative correlation between the proliferation cell index and body mass index and serum C-reactive protein levels was also observed. CONCLUSION: The increase in the proliferation capacity of type 1 diabetic T lymphocytes was probably not caused by hyperglycemia and/or insulinopenia related to inadequate metabolic control.

scholarly journals Effects of Oral Liposomal Glutathione in Altering the Immune Responses Against Mycobacterium tuberculosis and the Mycobacterium bovis BCG Strain in Individuals With Type 2 Diabetes

2021 ◽  
Vol 11 ◽  
Author(s):  
Kimberly To ◽  
Ruoqiong Cao ◽  
Aram Yegiazaryan ◽  
James Owens ◽  
Timothy Nguyen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Mononuclear Cells ◽  
World Health ◽  
Therapeutic Effects ◽  
Peripheral Blood Mononuclear ◽  
Middle Income ◽  
Health Organization

The World Health Organization (WHO) has identified type 2 diabetes (T2DM) as a neglected, important, and re-emerging risk factor for tuberculosis (TB), especially in low and middle-income countries where TB is endemic. In this clinical trial study, oral liposomal glutathione supplementation (L-GSH) or placebo was given to individuals with T2DM to investigate the therapeutic effects of L-GSH supplementation. We report that L-GSH supplementation for 3 months in people with T2DM was able to reduce the levels of oxidative stress in all blood components and prevent depletion of glutathione (GSH) in this population known to be GSH deficient. Additionally, L-GSH supplementation significantly reduced the burden of intracellular mycobacteria within in vitro granulomas generated from peripheral blood mononuclear cells (PBMCs) of T2DM subjects. L-GSH supplementation also increased the levels of Th1-associated cytokines, IFN-γ, TNF-α, and IL-2 and decreased levels of IL-6 and IL-10. In conclusion our studies indicate that oral L-GSH supplementation in individuals with T2DM for three months was able to maintain the levels of GSH, reduce oxidative stress, and diminish mycobacterial burden within in vitro generated granulomas of diabetics. L-GSH supplementation for 3 months in diabetics was also able to modulate the levels of various cytokines.

scholarly journals Effects of Low-Dose Atorvastatin on the Peripheral BloodMononuclear Cell Secretion of Angiogenic Factors in Type 2 Diabetes

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1885
Author(s):  
Anna Wesołowska ◽  
Hanna Winiarska ◽  
Jakub Owoc ◽  
Magdalena Borowska ◽  
Joanna Domagała ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Mononuclear Cells ◽  
Diabetic Patients ◽  
Cell Secretion ◽  
Peripheral Blood Mononuclear ◽  
Antiangiogenic Activity ◽  
Patients With Diabetes ◽  
Culture Supernatants

The aim of this study was to investigate the influence of statins on the secretion of angiogenesis mediators by the peripheral blood mononuclear cells (PBMCs) derived from patients suffering from type 2 diabetes. The study group comprised 30 participants and included: 10 statin-treated patients with diabetes, 10 statin-free diabetic subjects, and 10 statin-free non-diabetic individuals. PBMCs isolated from the blood were cultured in vitro in standard conditions and in an environment mimicking hyperglycemia. Culture supernatants were evaluated for VEGF, MCP-1, Il-10, and Il-12 by flow cytometry using commercial BDTM. Cytometric Bead Array tests. The secretion of VEGF, MCP-1 and Il-12 by PBMCs, cultured both in standard and hyperglycemic conditions, was significantly lower in the statin-treated patients with type 2 diabetes in comparison with the statin-free diabetic patients. Conversely, the secretion of Il-10 was higher in the statin-treated than in the statin-free diabetic patients. VEGF, MCP-1 and Il-12 levels in PBMCs supernatants from the glucose-containing medium were higher than those from the standard medium in each of the diabetic groups. The results of the study suggest that statins in low doses exhibit an antiangiogenic activity, reducing the secretion of potent proangiogenic factors, such as VEGF and MCP-1, and increasing the secretion of antiangiogenic Il-10 by PBMCs, also under hyperglycemic conditions characteristic for type 2 diabetes.

scholarly journals Elevated Nitric Oxide Production in Children with Malarial Anemia: Hemozoin-Induced Nitric Oxide Synthase Type 2 Transcripts and Nitric Oxide in Blood Mononuclear Cells

2004 ◽  
Vol 72 (8) ◽  
pp. 4868-4873 ◽  
Author(s):  
Christopher C. Keller ◽  
Peter G. Kremsner ◽  
James B. Hittner ◽  
Mary A. Misukonis ◽  
J. Brice Weinberg ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
No Production ◽  
Peripheral Blood Mononuclear ◽  
No Formation ◽  
Blood Mononuclear Cells ◽  
Malarial Anemia

ABSTRACT Experiments outlined here investigate the role of nitric oxide (NO) in the pathogenesis of Plasmodium falciparum-induced malarial anemia (MA). The results show that ex vivo and in vitro NO synthase (NOS) activity in peripheral blood mononuclear cells (PBMCs) is significantly elevated in children with MA and inversely associated with hemoglobin levels. Additional experiments using PBMCs from non-malaria-exposed donors demonstrate that physiologic amounts of P. falciparum-derived hemozoin augment NOS type 2 (NOS2) transcripts and NO production. Results of these experiments illustrate that elevated NO production in children with MA is associated with decreased hemoglobin concentrations and that hemozoin can induce NOS2-derived NO formation in cultured blood mononuclear cells.

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