scholarly journals Applications of vibrational spectroscopy in the study of flavin-based photoactive proteins

2011 ◽  
Vol 25 (6) ◽  
pp. 261-269 ◽  
Author(s):  
Jiang Li ◽  
Teizo Kitagawa
Keyword(s):  
Signal Transduction ◽  
Raman Spectroscopy ◽  
Blue Light ◽  
Reaction Mechanisms ◽  
Flavin Adenine Dinucleotide ◽  
Spectroscopic Studies ◽  
Biological Functions ◽  
Light Sensing ◽  
Infrared And Raman Spectroscopy ◽  
Sensitive Tool

Flavin cofactor is known to perform diverse biological functions. Recently, its role as a photoreceptor has been identified. So far, three classes of photoactive flavoproteins have been recognized: phototropin with LOV (Light, Oxygen and Voltage) domain, blue light sensory protein with BLUF (Blue Light sensing Using Flavin adenine dinucleotide) domain and photolyase/cryptochrome protein with PHR (Photolyase Homology Region) domain. Photochemistry of flavin is the key to unravel the reaction mechanisms of photoactive flavoproteins in their biological functions such as DNA repair or signal transduction. Vibrational (Infrared and Raman) spectroscopy is a useful and sensitive tool to investigate the photochemistry of flavin in protein environments and has significantly contributed to elucidate the reaction mechanisms of these photoactive proteins. This study will survey recent advances in vibrational spectroscopic studies on this topic and remaining questions to be answered.

Phase transitions in adamantane derivatives: 1-fluoroadamantane

1991 ◽  
Vol 69 (11) ◽  
pp. 1758-1765 ◽  
Author(s):  
Nancy T. Kawai ◽  
Denis F. R. Gilson ◽  
Ian S. Butler
Keyword(s):  
Phase Transition ◽  
Raman Spectroscopy ◽  
High Pressures ◽  
Spectroscopic Studies ◽  
Phase Behaviour ◽  
Slow Heating ◽  
Adamantane Derivatives ◽  
Scanning Calorimetry ◽  
Line Narrowing ◽  
Infrared And Raman Spectroscopy

The solid-state phase behaviour of 1-fluoroadamantane (1-C10H15F) has been investigated by differential scanning calorimetry, and by infrared and Raman spectroscopy. The phase transition occurs at 227 K on cooling and at 231 K upon heating, with average ΔHt and ΔSt of 1.65 kJ mol−1 and 7.3 J K−1 mol−1. The transition was also apparent in the low-temperature IR and Raman spectra by a sudden line narrowing, and characteristic spectral features of each phase were identified. Both phases I and II were disordered, and further cooling of phase II resulted in the formation of a glass. Attempts to induce a glass transition (predicted to occur at ~90 K) by slow heating were unsuccessful. Vibrational spectroscopic studies at high pressures showed a phase transition at 3.3 ± 1.0 kbar, upon compression. The phases formed under pressure were the same as those induced by lowering temperature. Key words: high-pressure infrared and Raman spectroscopy, phase transition, plastic crystal, orientational disorder.

Identification of filler substances in emeralds by infrared and Raman spectroscopy

2000 ◽  
Vol 26 (1) ◽  
pp. 501-520 ◽  
Author(s):  
L. Kiefert ◽  
H.A. Hänni ◽  
J-P. Chalain ◽  
W. Weber
Keyword(s):  
Raman Spectroscopy ◽  
Infrared And Raman Spectroscopy

scholarly journals Multivariate approach for the authentication of vanilla using infrared and Raman spectroscopy

2021 ◽  
Vol 141 ◽  
pp. 110196
Author(s):  
Ana M. Jiménez-Carvelo ◽  
Margherita Tonolini ◽  
Orla McAleer ◽  
Luis Cuadros-Rodríguez ◽  
Daniel Granato ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Multivariate Approach ◽  
Infrared And Raman Spectroscopy

scholarly journals Authentication of the Botanical and Geographical Origin and Detection of Adulteration of Olive Oil Using Gas Chromatography, Infrared and Raman Spectroscopy Techniques: A Review

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1565
Author(s):  
Eleni Kakouri ◽  
Panagiota-Kyriaki Revelou ◽  
Charalabos Kanakis ◽  
Dimitra Daferera ◽  
Christos S. Pappas ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Raman Spectroscopy ◽  
Olive Oil ◽  
Nutritional Value ◽  
Review Paper ◽  
Geographical Origin ◽  
Analytical Techniques ◽  
Volatile Fraction ◽  
Olive Oils ◽  
Infrared And Raman Spectroscopy

Olive oil is among the most popular supplements of the Mediterranean diet due to its high nutritional value. However, at the same time, because of economical purposes, it is also one of the products most subjected to adulteration. As a result, authenticity is an important issue of concern among authorities. Many analytical techniques, able to detect adulteration of olive oil, to identify its geographical and botanical origin and consequently guarantee its quality and authenticity, have been developed. This review paper discusses the use of infrared and Raman spectroscopy as candidate tools to examine the authenticity of olive oils. It also considers the volatile fraction as a marker to distinguish between different varieties and adulterated olive oils, using SPME combined with gas chromatography technique.

scholarly journals Blue light sensing in higher plants.

2001 ◽  
Vol 276 (20) ◽  
pp. 17620
Author(s):  
John M. Christie ◽  
Winslow R. Briggs
Keyword(s):  
Blue Light ◽  
Higher Plants ◽  
Light Sensing

Techniques to examine nucleotide binding by pseudokinases

2013 ◽  
Vol 41 (4) ◽  
pp. 975-980 ◽  
Author(s):  
Isabelle S. Lucet ◽  
Jeffrey J. Babon ◽  
James M. Murphy
Keyword(s):  
Signal Transduction ◽  
Drug Targets ◽  
Nucleotide Binding ◽  
Biological Functions ◽  
Binding Properties ◽  
Catalytic Activities ◽  
Functional Studies ◽  
Signalling Molecules ◽  
Advantages And Disadvantages ◽  
Human Kinome

Approximately 10% of the human kinome has been classified as pseudokinases due to the absence of one or more of three motifs known to play key roles in the catalytic activities of protein kinases. Structural and functional studies are now emerging, reclassifying this ‘dead’ kinase family as essential signalling molecules that act as crucial modulators of signal transduction. This raises the prospect that pseudokinases may well represent an as-yet-unexplored class of drug targets. However, the extent to which nucleotide binding and catalytic activity contribute to the biological functions of pseudokinases remains an area of great controversy. In the present review, we discuss the advantages and disadvantages of the different methods employed to characterize the nucleotide-binding properties and activity of pseudokinases.

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