scholarly journals Histopathological Features in a Case of Peters' Anomaly with Acquired Corneal Staphyloma

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Kumi Shirai ◽  
Yuka Okada ◽  
Yasushi Nakamura ◽  
Shizuya Saika
Keyword(s):  
Collagen Type ◽  
Corneal Stroma ◽  
Posterior Part ◽  
Collagen Type I ◽  
Type I ◽  
Peters Anomaly ◽  
Hematoxylin And Eosin ◽  
Corneal Staphyloma ◽  
The Right ◽  
Peripheral Cornea

We report a case of corneal staphyloma histologically diagnosed as caused by Peters' anomaly. A 62-year-old male had a protruding opaque vascularized cornea that began to bulge from six months ago in the right eye. Since his right eye was blind and he wanted us to remove the eyeball for cosmetic improvement, we enucleated the affected eye. The enucleated tissue was fixed in formalin and embedded in paraffin for histological examination. Hematoxylin and eosin staining showed that the cornea lacked the posterior part of the corneal stroma and Descemet's membrane in the central region and the entire corneal endothelium. The corneal epithelium was keratinized. Collagen type I was strongly positive in peripheral cornea and weakly in protruding stroma. The cells labeled by antibodies againstαSMA were scattered in the entire corneal stroma. As judged by the histological findings, the eye with the central corneal staphyloma was diagnosed as Peters' anomaly.

scholarly journals Relative rates of biosynthesis of collagen type I, type V and type VI in calf cornea

1991 ◽  
Vol 274 (2) ◽  
pp. 615-617 ◽  
Author(s):  
P Kern ◽  
M Menasche ◽  
L Robert
Keyword(s):  
Type I Collagen ◽  
Collagen Type ◽  
Corneal Stroma ◽  
Collagen Type I ◽  
Type I ◽  
Type Vi Collagen ◽  
Sds Page ◽  
Proline Incorporation ◽  
Type V

The biosynthesis of type I, type V and type VI collagens was studied by incubation of calf corneas in vitro with [3H]proline as a marker. Pepsin-solubilized collagen types were isolated by salt fractionation and quantified by SDS/PAGE. Expressed as proportions of the total hydroxyproline solubilized, corneal stroma comprised 75% type I, 8% type V and 17% type VI collagen. The rates of [3H]proline incorporation, linear up to 24 h for each collagen type, were highest for type VI collagen and lowest for type I collagen. From pulse-chase experiments, the calculated apparent half-lives for types I, V and VI collagens were 36 h, 10 h and 6 h respectively.

EFFECT OF PURIFIED ALGINATE MICROCAPSULES ON THE REGENERATION OF CHONDROCYTES

2012 ◽  
Vol 24 (03) ◽  
pp. 185-195 ◽  
Author(s):  
Ji Hye Hwang ◽  
On You Kim ◽  
A Ram Kim ◽  
Ji Yeon Bae ◽  
Su Mi Jeong ◽  
...  
Keyword(s):  
Cell Viability ◽  
Collagen Type ◽  
Cartilage Regeneration ◽  
Collagen Type I ◽  
Tissue Growth ◽  
Cartilage Tissue ◽  
Type I ◽  
Hematoxylin And Eosin ◽  
Safranin O

Adult articular cartilage tissue has poor capability of self-repair. Therefore, a variety of tissue engineering approaches are motivated by the clinical need for articular repair. Alginate has been used as a biomaterial for cartilage regeneration. The alginate is a natural polymer that is extracted from seaweeds and purification. However, the main drawback is the immune rejection in vivo. To overcome this problem, we have developed the biocompability of alginate using modified Korbutt method. After alginate was purified, purified alginate microcapsules were used in cartilage regeneration. Chondrocytes were seeded in purified and nonpurified alginate microcapsules, and then cell viability, proliferation and phenotype were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. Reverse transcriptase-polymerase chain reaction (RT-PCR) was conducted to confirm mRNA expression on collagen type I and collagen type II for chondrocytes phenotype. Hematoxylin and eosin (H&E) and Safranin-O histological staining showed tissue growth at the interface during the first 10 days. In this study, chondrocytes in purified alginate microcapsules had higher cell viability, proliferation and more phenotype expression than those in nonpurified alginate microcapsules. The results suggest that the purified alginate microcapsule is useful for cartilage regeneration.

scholarly journals The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Menglei Wang ◽  
Meixuan Li ◽  
Pisong Yan ◽  
Qiang Luo ◽  
Yu Zhang ◽  
...  
Keyword(s):  
High Intensity ◽  
Focused Ultrasound ◽  
Collagen Type ◽  
Anterior Segment ◽  
Collagen Type I ◽  
High Intensity Focused Ultrasound ◽  
Type I ◽  
Corneal Cells ◽  
The Right ◽  
Corneal Collagen

Purpose.To evaluate the safety of high-intensity focused ultrasound keratoplasty as a treatment for presbyopia by examining its effect on the rabbit anterior segment.Methods.The right corneas of 36 New Zealand rabbits were treated with HIFU keratoplasty. The animals were sacrificed at 1, 7, 15, 30, 60, and 90 days after operation. Collagen type I, MMP-2, and MMP-9 were evaluated using immunohistochemistry. For the detection of apoptosis, the TUNEL method was applied. The SOD and MDA levels were analyzed with assay kits.Results.Collagen type I, MMP-2, and MMP-9 levels were altered after the operation but returned to normal within 90 days. The apoptotic index (AI) of the corneal cells decreased from 1 to 30 days gradually. No apoptosis was observed in the epithelial cells of the lens, and the SOD and MDA levels were normal at any time point.Conclusion.After HIFU keratoplasty, the histomorphology of the cornea changed, the corneal collagen type I levels decreased, the corneal MMP-2 and MMP-9 levels increased, and the corneal cells underwent apoptosis for a period of time. Ninety days after the operation, the levels returned to normal, and the lenses were not affected. Thus, HIFU presents good biological safety for eyes.

scholarly journals Calcium Regulation on the Atrial Regional Difference of Collagen Production Activity in Atrial Fibrogenesis

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 686
Author(s):  
Cheng-Chih Chung ◽  
Yung-Kuo Lin ◽  
Yao-Chang Chen ◽  
Yu-Hsun Kao ◽  
Yung-Hsin Yeh ◽  
...  
Keyword(s):  
Heart Failure ◽  
Transient Receptor Potential ◽  
Collagen Type ◽  
Receptor Potential ◽  
Collagen Type I ◽  
Type I ◽  
Collagen Production ◽  
Type Iii ◽  
Transient Receptor ◽  
The Right

Background: Atrial fibrosis plays an important role in the genesis of heart failure and atrial fibrillation. The left atrium (LA) exhibits a higher level of fibrosis than the right atrium (RA) in heart failure and atrial arrhythmia. However, the mechanism for the high fibrogenic potential of the LA fibroblasts remains unclear. Calcium (Ca2+) signaling contributes to the pro-fibrotic activities of fibroblasts. This study investigated whether differences in Ca2+ homeostasis contribute to differential fibrogenesis in LA and RA fibroblasts. Methods: Ca2+ imaging, a patch clamp assay and Western blotting were performed in isolated rat LA and RA fibroblasts. Results: The LA fibroblasts exhibited a higher Ca2+ entry and gadolinium-sensitive current compared with the RA fibroblasts. The LA fibroblasts exhibited greater pro-collagen type I, type III, phosphorylated Ca2+/calmodulin-dependent protein kinase II (CaMKII), phosphorylated phospholipase C (PLC), stromal interaction molecule 1 (STIM1) and transient receptor potential canonical (TRPC) 3 protein expression compared with RA fibroblasts. In the presence of 1 mmol/L ethylene glycol tetra-acetic acid (EGTA, Ca2+ chelator), the LA fibroblasts had similar pro-collagen type I, type III and phosphorylated CaMKII expression compared with RA fibroblasts. Moreover, in the presence of KN93 (a CaMKII inhibitor, 10 μmol/L), the LA fibroblasts had similar pro-collagen type I and type III compared with RA fibroblasts. Conclusion: The discrepancy of phosphorylated PLC signaling and gadolinium-sensitive Ca2+ channels in LA and RA fibroblasts induces different levels of Ca2+ influx, phosphorylated CaMKII expression and collagen production.

scholarly journals PCL Scaffold Combined with Rat Tail Collagen Type I to Reduce Keratocyte Differentiation and Prevent Corneal Stroma Fibrosis after Injury.

2021 ◽  
Author(s):  
Wenhan Xu ◽  
Bin Kong ◽  
Huatao Xie ◽  
Weijian Liu ◽  
Sheng Liu ◽  
...  
Keyword(s):  
Composite Material ◽  
Collagen Type ◽  
Corneal Stroma ◽  
Collagen Type I ◽  
Type I ◽  
Corneal Tissue ◽  
Self Healing ◽  
Corneal Injury ◽  
3D Composite ◽  
Rat Tail

Abstract The cornea is one of the major refractive eye components with significant functions, and its transparency is essential for clear vision. With regard to corneal injury, the corneal epithelium has a strong self-healing ability, while the corneal stroma is not capable of total self-repair. Therefore, preventing fibrosis and reducing keratocyte differentiation after injury have always been a challenge. The severe shortage of donor corneas for transplantation and transplant rejection prompted the development of corneal tissue engineering. In this study, we fabricated a poly(ε-caprolactone) (PCL) microfibrous scaffold and infused the scaffold with rat tail collagen type I to obtain a 3D composite material. The PCL/collagen scaffold was designed to fabricate an optimal construct that simulates the stromal structure with properties that are most similar to the native cornea. The PCL scaffold has good mechanical properties, and infusion with rat tail collagen type I improved its biocompatibility. The results demonstrate that 3D composite material could reduce keratocyte differentiation, help achieve regular collagen distribution, and promote corneal repair.

The Ferret as a Surgical Model for Vocal Fold Scar Creation and Treatment

2018 ◽  
Vol 127 (3) ◽  
pp. 146-154 ◽  
Author(s):  
Haruka Kodama ◽  
Yoshihiko Kumai ◽  
Kohei Nishimoto ◽  
Yutaka Toya ◽  
Satoru Miyamaru ◽  
...  
Keyword(s):  
Vocal Fold ◽  
Collagen Type ◽  
Experimental Treatment ◽  
Muscle Layer ◽  
Collagen Type I ◽  
Type I ◽  
India Ink ◽  
Vocal Fold Scar ◽  
Female Wistar Rats ◽  
The Right

Objectives: To develop a vocal fold (VF) scarring procedure in the ferret, characterize the scars histologically, and test the injectability of the lamina propria (LP). Secondarily, to compare laryngeal anatomy of the ferret with rat and rabbit. Materials and Methods: The larynges of 18 male ferrets were prepared by unilateral scarring, and normal larynges from 6 female Wistar rats and 5 male albino rabbits were used for comparative purposes. For scarring, the right VF were electrocauterized, ablating the entire LP. Prior to harvesting the larynges at 4 and 16 weeks, each ferret was re-anesthetized, and in 3 animals, India ink was injected into the LPs of both normal and scarred VFs. Results: Laryngoscopic methods and instrumentation for precise visualization, scarring, and injection were developed. The scarred VFs had reduced hyaluronic acid and increased collagen type I, III, and fibronectin compared with normal VFs. The 2 timepoints (4 and 16 weeks) differed significantly only in collagen type III level (levels were higher at 4 weeks). Injected ink migrated from scarred LP to muscle layer just beneath the scarred tissue 3 hours after injection. Conclusion: The ferret is a promising species for creation and experimental treatment of vocal fold scar.

scholarly journals Collagen type I and type V are present in the same fibril in the avian corneal stroma.

1988 ◽  
Vol 106 (3) ◽  
pp. 999-1008 ◽  
Author(s):  
D E Birk ◽  
J M Fitch ◽  
J P Babiarz ◽  
T F Linsenmayer
Keyword(s):  
Monoclonal Antibodies ◽  
Collagen Type ◽  
Corneal Stroma ◽  
Collagen Type I ◽  
Collagen Molecule ◽  
Type I ◽  
Collagen Types ◽  
Type V Collagen ◽  
Fibril Structure ◽  
Type V

The distribution, supramolecular form, and arrangement of collagen types I and V in the chicken embryo corneal stroma were studied using electron microscopy, collagen type-specific monoclonal antibodies, and a preembedding immunogold method. Double-label immunoelectron microscopy with colloidal gold-tagged monoclonal antibodies was used to simultaneously localize collagen type I and type V within the chick corneal stroma. The results definitively demonstrate, for the first time, that both collagens are codistributed within the same fibril. Type I collagen was localized to striated fibrils throughout the corneal stroma homogeneously. Type V collagen could be localized only after pretreatment of the tissue to partially disrupt collagen fibril structure. After such pretreatments the type V collagen was found in regions where fibrils were partially dissociated and not in regions where fibril structure was intact. When pretreated tissues were double labeled with antibodies against types I and V collagen coupled to different size gold particles, the two collagens colocalized in areas where fibril structure was partially disrupted. Antibodies against type IV collagen were used as a control and were nonreactive with fibrils. These results indicate that collagen types I and V are assembled together within single fibrils in the corneal stroma such that the interaction of these collagen types within heterotypic fibrils masks the epitopes on the type V collagen molecule. One consequence of the formation of such heterotypic fibrils may be the regulation of corneal fibril diameter, a condition essential for corneal transparency.

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