scholarly journals Metabolomics Analysis ofCistus monspeliensisLeaf Extract on Energy Metabolism Activation in Human Intestinal Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Yoichi Shimoda ◽  
Junkyu Han ◽  
Kiyokazu Kawada ◽  
Abderrazak Smaoui ◽  
Hiroko Isoda
Keyword(s):  
Energy Metabolism ◽  
Tca Cycle ◽  
Point Of View ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Important Process ◽  
Antioxidant Ability ◽  
Atp Production ◽  
Human Intestinal Epithelial Cells ◽  
Human Intestinal Cells

Energy metabolism is a very important process to improve and maintain health from the point of view of physiology. It is well known that the intracellular ATP production is contributed to energy metabolism in cells.Cistus monspeliensisis widely used as tea, spices, and medical herb; however, it has not been focusing on the activation of energy metabolism. In this study,C. monspeliensiswas investigated as the food resources by activation of energy metabolism in human intestinal epithelial cells.C. monspeliensisextract showed high antioxidant ability. In addition, the promotion of metabolites of glycolysis and TCA cycle was induced byC. monspeliensistreatment. These results suggest thatC. monspeliensisextract has an ability to enhance the energy metabolism in human intestinal cells.

scholarly journals A pumpless body-on-a-chip model using a primary culture of human intestinal cells and a 3D culture of liver cells

Lab on a Chip ◽  
2018 ◽  
Vol 18 (14) ◽  
pp. 2036-2046 ◽  
Author(s):  
Huanhuan Joyce Chen ◽  
Paula Miller ◽  
Michael L. Shuler
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Intestinal Epithelial Cells ◽  
3D Culture ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells ◽  
Improved Model ◽  
Drug Studies ◽  
Human Intestinal Cells

A pumpless GI–Liver system using primary human intestinal epithelial cells serves as an improved model for drug studies.

scholarly journals Influence of Exposure Time on Gene Expression by Human Intestinal Epithelial Cells Exposed to Lactobacillus acidophilus

2012 ◽  
Vol 78 (14) ◽  
pp. 5028-5032 ◽  
Author(s):  
Sarah O'Flaherty ◽  
Todd R. Klaenhammer
Keyword(s):  
Gene Expression ◽  
Lactobacillus Acidophilus ◽  
Target Genes ◽  
Intestinal Epithelial Cells ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Content Type ◽  
Temporal Gene Expression ◽  
Human Intestinal Epithelial Cells ◽  
Human Intestinal Cells

ABSTRACTAnalysis of global temporal gene expression by human intestinal cells when exposed toLactobacillus acidophilusrevealed induction of immune-related pathways and NF-κB target genes after a 1-h exposure, compared to a 4- or 8-h exposure. Additionally, anL. acidophilusderivative expressing covalently bound flagellin resulted in increased induction ofil8,cxc1, andcxcl2compared to the parentL. acidophilus.

scholarly journals Curcumae Radix Extract Reduces Beta-Amyloid (Αβ) and Tau Protein Through Increased Mitochondrial Respiration

2021 ◽  
Author(s):  
Seong lae Jo ◽  
Hyun Yang ◽  
Jun H. Heo ◽  
Sang R. Lee ◽  
Hye Won Lee ◽  
...  
Keyword(s):  
Brain Tumor ◽  
Energy Metabolism ◽  
Neurodegenerative Diseases ◽  
Mitochondrial Respiration ◽  
Metabolic Flux ◽  
Tca Cycle ◽  
Basal Respiration ◽  
Atp Production ◽  
Inflammatory Conditions ◽  
Brain Tumor Cells

Abstract Background: Neurodegenerative diseases are increasingly being studied owing to the increasing proportion of the aging population. Several potential compounds have been studied to prevent neurodegenerative diseases, one of which is Curcumae Radix that is known to be beneficial for inflammatory conditions, metabolic syndrome, and various types of pain. However, it is not well studied and its influence on energy metabolism in neurodegenerative diseases is unclear. We focused on the relationship between neurodegenerative diseases and energy metabolism through Curcumae Radix extract in an animal model. Methods: Mice were treated with Curcumae Radix extract for 5 weeks orally 5 times in a week (50 mg/kg body weight). Murine delayed brain tumor (DBT) cells were supplemented with Curcumae Radix extract. We monitored the neurodegenerative makers and metabolic indicators using Western blotting and qRT-PCR and then assessed the cellular glycolysis and mitochondrial respiration through metabolic flux assay.Results: Low expression levels of Alzheimer’s disease-related markers were observed after treatment with Curcumae Radix extract. It was determined through the pAMPK/AMPK ratio that the ATP state was sufficient in the cerebrum and brain tumor cells. With this, an increase in glycolysis would be expected as glucose is the main energy source of the brain. However, glycolysis-related genes and the extracellular acidification rate showed that glycolysis decreased. Despite this, basal respiration and ATP production through mitochondrial respiration and increased TCA cycle and OXPHOS-related genes were observed in the Curcumae Radix group. Conclusions: In neurodegenerative diseases involving mitochondrial dysfunction, Curcumae Radix may act as a metabolic modulator of brain health to treat and prevent these diseases.

scholarly journals Giardia lamblia-Induced Changes in Gene Expression in Differentiated Caco-2 Human Intestinal Epithelial Cells

2005 ◽  
Vol 73 (12) ◽  
pp. 8204-8208 ◽  
Author(s):  
Katarina Roxström-Lindquist ◽  
Emma Ringqvist ◽  
Daniel Palm ◽  
Staffan Svärd
Keyword(s):  
Intestinal Epithelium ◽  
Giardia Lamblia ◽  
Intestinal Inflammation ◽  
Expression Patterns ◽  
Enteric Pathogens ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Innate And Adaptive Immunity ◽  
Human Intestinal Epithelial Cells ◽  
Induced Changes

ABSTRACT The parasitic protozoan Giardia lamblia is a worldwide cause of diarrhea, but the mechanism of disease remains elusive. The parasite colonizes the small intestinal epithelium, known to be a sensor for the presence of enteric pathogens, without invading or causing severe inflammation. In this study we investigated the epithelial cell response to G. lamblia. Differentiated Caco-2 cells were infected with G. lamblia isolate WB-A11, and the transcriptome of the intestinal cells was analyzed after 1.5, 6, and 18 h of interaction, using oligonucleotide microarrays. A large number of genes displayed changed expression patterns, showing the complexity of the interaction between G. lamblia and intestinal cells. A novel chemokine profile (CCL2, CCL20, CXCL1, CXCL2, and CXCL3) was induced that was different from the response induced by enteric pathogens causing intestinal inflammation. Several genes involved in stress regulation changed their expression. These findings indicate that the intestinal epithelium senses the G. lamblia infection, and this is important for induction of innate and adaptive immunity. The induced stress response can be important in the pathogenesis.

scholarly journals The food additive E171 and titanium dioxide nanoparticles indirectly alter the homeostasis of human intestinal epithelial cells in vitro

2019 ◽  
Vol 6 (5) ◽  
pp. 1549-1561 ◽  
Author(s):  
Marie Dorier ◽  
David Béal ◽  
Céline Tisseyre ◽  
Caroline Marie-Desvergne ◽  
Muriel Dubosson ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Titanium Dioxide Nanoparticles ◽  
Food Additive ◽  
Repeated Exposure ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

Repeated exposure to E171 or TiO2-NPs, in vitro, induce moderate inflammation and mucus secretion in intestinal cells.

scholarly journals Rotavirus Infection Induces Cytoskeleton Disorganization in Human Intestinal Epithelial Cells: Implication of an Increase in Intracellular Calcium Concentration

2000 ◽  
Vol 74 (22) ◽  
pp. 10801-10806 ◽  
Author(s):  
Jean-Philippe Brunet ◽  
Nathalie Jourdan ◽  
Jacqueline Cotte-Laffitte ◽  
Catherine Linxe ◽  
Monique Géniteau-Legendre ◽  
...  
Keyword(s):  
Calcium Concentration ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Cytokeratin 18 ◽  
Structural Alterations ◽  
Rotavirus Infection ◽  
Differentiated Cells ◽  
Human Intestinal Epithelial Cells

ABSTRACT Rotavirus infection is the most common cause of severe infantile gastroenteritis worldwide. In vivo, rotavirus exhibits a marked tropism for the differentiated enterocytes of the intestinal epithelium. In vitro, differentiated and undifferentiated intestinal cells can be infected. We observed that rotavirus infection of the human intestinal epithelial Caco-2 cells induces cytoskeleton alterations as a function of cell differentiation. The vimentin network disorganization detected in undifferentiated Caco-2 cells was not found in fully differentiated cells. In contrast, differentiated Caco-2 cells presented Ca2+-dependent microtubule disassembly and Ca2+-independent cytokeratin 18 rearrangement, which both require viral replication. We propose that these structural alterations could represent the first manifestations of rotavirus-infected enterocyte injury leading to functional perturbations and then to diarrhea.

Infection of cultured human intestinal cells by monkey RRV and human Wa rotavirus as a function of intestinal epithelial cell differentiation

1995 ◽  
Vol 146 (5) ◽  
pp. 325-331 ◽  
Author(s):  
N. Jourdan ◽  
J. Cotte Laffitte ◽  
F. Forestier ◽  
A.L. Servin ◽  
A.M. Quéro
Keyword(s):  
Cell Differentiation ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Epithelial Cell Differentiation ◽  
Human Intestinal Cells

Induction of apoptosis in HT29 human intestinal epithelial cells by the cytotoxic enterotoxin ofAeromonas hydrophila

2001 ◽  
Vol 79 (4) ◽  
pp. 525-531 ◽  
Author(s):  
Rosabel M Falcón ◽  
Hernandes F Carvalho ◽  
Paulo Pinto Joazeiro ◽  
Maria S. Viccari Gatti ◽  
Tomomasa Yano
Keyword(s):  
Aeromonas Hydrophila ◽  
Gel Electrophoresis ◽  
Light And Electron Microscopy ◽  
Intestinal Cells ◽  
Agarose Gel ◽  
Intestinal Epithelial ◽  
Agarose Gel Electrophoresis ◽  
Nuclear Condensation ◽  
Gastrointestinal Infections ◽  
Human Intestinal Cells

The cytotoxic enterotoxin produced by Aeromonas hydrophila is considered to be the main virulence factor in gastrointestinal infections mediated by this pathogen. In this study, we examined the morphological and apoptotic effects of this toxin on HT29 cells, using light and electron microscopy in situ, as well as agarose gel electrophoresis of cell DNA. Cells treated with the cytotoxic enterotoxin became round and lost their polarity as well as their adhesion to each other and to the substrate. Cytoplasmic blebbing and nuclear condensation also occurred. DNA fragmentation was detected by TUNEL labelling and agarose gel electrophoresis. These results show that the cytotoxic enterotoxin of A. hydrophila can induce apoptosis in human intestinal cells in culture.Key words: Aeromonas hydrophila, cytotoxic enterotoxin, aerolysin, apoptosis, human intestinal cells.

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