scholarly journals Apolar Compounds in Seaweeds from Fernando de Noronha Archipelago (Northeastern Coast of Brazil)

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Leandro De Santis Ferreira ◽  
Izabel Cristina Casanova Turatti ◽  
Norberto Peporine Lopes ◽  
Thais Guaratini ◽  
Pio Colepicolo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Comparative Study ◽  
Mass Spectrometer ◽  
Polluted Area ◽  
Hyphenated Techniques ◽  
Human Influence ◽  
Fernando De Noronha ◽  
Northeastern Coast ◽  
Eleven Species

Hyphenated techniques of gas chromatography coupled to mass spectrometer were used to determine fatty acids in eleven species of seaweeds from Fernando de Noronha archipelago. The main compounds detected in all studied species were the alcohol phytol and the fatty acids 14 : 0; 15 : 0; 16 : 0; 18 : 0; 18 : 1 n9; 18 : 2 Δ9,12; 20 : 4; 20 : 5. These fatty acids are commonly found in seaweeds present in warm regions. Thus, we found no specificity in the presence of a particular set of fatty acids and the studied species indicating that they are not useful as taxonomic indicators. However, they could be used in a comparative study with algae found in polluted area because many of the studied seaweeds are widespread and Fernando de Noronha has low human influence.

scholarly journals Comparative study for analysis of carbohydrates in biological samples

2021 ◽  
Author(s):  
Martin Meyer ◽  
Lidia Montero ◽  
Sven W. Meckelmann ◽  
Oliver J. Schmitz
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Comparative Study ◽  
Mass Spectrometer ◽  
Biological Samples ◽  
Multiple Reaction Monitoring ◽  
Reversed Phase ◽  
Separation Performance ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid

AbstractThis work presents a comparative study for the analysis of carbohydrates for four common chromatographic methods, each coupled to mass spectrometry. Supercritical fluid chromatography (SFC), hydrophilic interaction liquid chromatography (HILIC), reversed-phase liquid chromatography (RP-LC) and gas chromatography (GC) with detection by triple quadrupole mass spectrometer (QqQ-MS) are compared. It is shown that gas chromatography and reversed-phase liquid chromatography, each after derivatisation, are superior to the other two methods in terms of separation performance. Furthermore, comparing the different working modes of the mass spectrometer, it can be determined that a targeted analysis, i.e. moving from full scan to single ion monitoring (SIM) and multiple reaction monitoring (MRM), results in an improvement in the sensitivity as well as the repeatability of the method, which has deficiencies especially in the analysis using HILIC. Overall, RP-LC–MS in MRM after derivatisation with 1-phenyl-3-methyl-5-pyrazolone (PMP) proved to be the most suitable method in terms of separation performance, sensitivity and repeatability for the analysis of monosaccharides. Detection limits in the nanomolar range were achieved, which corresponds to a mass concentration in the low µg/L range. The applicability of this method to different biological samples was investigated with various herbal liquors, pectins and a human glycoprotein. Graphical abstract

Proximal chemical evaluation and fatty acid in the chicatana ant and jumile

2020 ◽  
pp. 1-8
Author(s):  
V.A. Lopez-Morales ◽  
J.A. Ariza-Ortega ◽  
J. Díaz-Reyes ◽  
D. Castañeda-Antonio ◽  
M.E. Ramos-Cassellis ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Refractive Index ◽  
Fatty Acid ◽  
Chemical Composition ◽  
Linolenic Acid ◽  
Mass Spectrometer ◽  
Chemical Evaluation ◽  
Alternative Sources ◽  
Instrumental Methods

In the state of Tlaxcala, Mexico, the chicatana ant (Atta mexicana) and jumile (Edessa spp.) are foods that the population regularly consumes and are alternative sources of nutrients. Therefore, this study aims to evaluate the proximal chemical composition (moisture, protein, lipids, ash and carbohydrates) and fatty acids (FA) in commercialised dehydrated chicatana ant and jumile using instrumental methods such as refractive index (RI) and gas chromatography coupled with mass spectrometer (GC-MS). Protein and lipids were predominant in chicatana ant and jumile (33.74% and 39.25%, and 32.00% and 39.00%, respectively). In insect oils, RI and GC-MS results demonstrated that chicatana ant oil contains a high saturated FA percentage (58.40%), with heneicosanoic acid (C21:0) being the predominant saturated FA (46.35%), while jumile oil presented a higher percentage of unsaturated acids (99.27%), with γ-linolenic acid being the predominant unsaturated FA (77.00%). Therefore, both insects are foods with important protein and FA percentage, with jumile containing a higher concentration of unsaturated FA, which is desirable in the diet.

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