Thymoquinone, theNigella sativaBioactive Compound, Prevents Circulatory Oxidative Stress Caused by 1,2-Dimethylhydrazine in Erythrocyte during Colon Postinitiation Carcinogenesis

We have performed this study to investigate the modulatory effect of thymoquinone (TQ), theNigella sativaactive compound, on erythrocyte lipid peroxidation and antioxidant status during 1,2-dimethylhydrazine- (DMH-) induced colon carcinogenesis after initiation in male Wistar rats. Rats exposed to DMH showed an increase of malondialdehyde and conjugated diene levels, and an augmentation of enzyme activities like catalase, glutathione peroxidase, and superoxide dismutase activities was also noted. The TQ pretreatment restored the parameters cited above to near-normal values. However, the posttreatment shows an activity similar as that presented by DMH. Therefore, our investigation revealed that TQ was a useful compound preventing DMH-induced erythrocyte damages.

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The subchronic effects of 3,4-methylendioxymethamphetamine on oxidative stress in rat brain

Archives of Biological Sciences ◽

10.2298/abs1403075s ◽

2014 ◽

Vol 66 (3) ◽

pp. 1075-1081

Author(s):

Ivan Simic ◽

Violeta Iric-Cupic ◽

Rada Vucic ◽

Marina Petrovic ◽

Violeta Mladenovic ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Rat Brain ◽

Wistar Rats ◽

Superoxide Radical ◽

Reduced Glutathione ◽

Oxidative Stress Markers ◽

Sod Activity ◽

Stress Markers ◽

Male Wistar Rats

The aim of the present study was to evaluate the subchronic effects of 3,4-methylenedioxymethamphetamine on several oxidative stress markers: index of lipid peroxidation (ILP), superoxide dismutase (SOD) activity, superoxide radical (O2.-) levels, and reduced glutathione (GSH) levels in the frontal cortex, striatum and hippocampus of the rat. The study included 64 male Wistar rats (200-250g). The animals were treated per os with of 5, 10, or 20 mg/kg of 3,4-methylenedioxymethamphetamine (MDMA) every day for 15 days. The subchronic administration of MDMA resulted in an increase in ILP, SOD and O2.-, and a decrease in GSH, from which we conclude that oxidative stress was induced in rat brain.

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Protective effect of chokeberry on chemical-induced oxidative stress in rat

Human & Experimental Toxicology ◽

10.1177/0960327110371697 ◽

2010 ◽

Vol 30 (3) ◽

pp. 199-208 ◽

Cited By ~ 16

Author(s):

Małgorzata Kujawska ◽

Ewa Ignatowicz ◽

Małgorzata Ewertowska ◽

Jan Oszmiański ◽

Jadwiga Jodynis-Liebert

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Wistar Rats ◽

Thiobarbituric Acid ◽

Protein Carbonyls ◽

Thiobarbituric Acid Reactive Substances ◽

Blood Leukocytes ◽

Microsomal Lipid Peroxidation ◽

Male Wistar Rats ◽

Chokeberry Juice

Male Wistar rats were treated with chokeberry juice per os, 10 mL/kg/day, for 28 days and a single intraperitoneal (i.p.) dose of N-nitrosodiethylamine (NDEA), 150 mg/kg, or carbon tetrachloride (CCl4), 2 ml/kg. The level of hepatic microsomal lipid peroxidation, expressed as thiobarbituric acid reactive substances (TBARS), was increased in animals dosed with NDEA and CCl4. Juice pretreatment resulted in a significant decrease in TBARS by 53% and 92%, respectively. In rats administered juice alone, 50% decrease in TBARS was noted. The activities of all antioxidant enzymes were decreased in the liver of rats administered either toxicant by 29%—52% as compared to controls. Juice pretreatment resulted in an increase in the activity of catalase, glutathione peroxidase and glutathione reductase by 117%, 56% and 44%, respectively, only in rats challenged with NDEA. Although no response of plasma protein carbonyls to both toxicants was observed, the pretreatment with juice caused a 55% decrease of this parameter in CCl4—dosed rats. DNA damage in blood leukocytes induced by either toxicant was slightly reduced, by 24%, in the rats pretreated with juice and administered NDEA. The results of the study showed that pretreatment with chokeberry juice confers some protection against chemical-induced oxidative stress.

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Sulforaphane response on aluminum-induced oxidative stress, alterations in sperm characterization and testicular histomorphometry in Wistar rats

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v13i8.7503 ◽

2020 ◽

Author(s):

Babatunde Ogunlade ◽

Sunday Adelakun ◽

Kingsley Iteire

Keyword(s):

Oxidative Stress ◽

Adult Male ◽

Antioxidant Status ◽

Wistar Rats ◽

Sperm Count ◽

Seminiferous Tubules ◽

Testicular Toxicity ◽

Significant Difference ◽

Male Wistar Rats ◽

Aluminum Trichloride

Background: The exposure of male individual to environmental toxicant is regarded as a channel that results in reduced sperm counts and infertility. Objective: This study investigated the ameliorative response of Sulforaphane (SFN) on Aluminum trichloride (AlCl3) induced testicular toxicity in adult male Wistar rats. Materials and Methods: A total of 32 adult male Wistar rats (180-200 gm between 8-10 wk) were divided into four groups (n = 8/each). Group A) received distilled water orally as placebo; Group B) received 100 mg/kgbw AlCl3 only orally; Group C) received 100 mg/kgbw AlCl3 and 100 mg/kgbw SFN orally; and Group D) received 100 mg/kgbw SFN only orally. After 28 days of experiment, animals underwent cervical dislocation, blood serum was obtained for analysis, and testes were harvested for biochemical assays, histology, hormonal profile, and sperm characterization. Results: The sperm parameters showed a significant difference within the AlCl3 only group compared with the control and SFN only groups (p = 0.02). However, AlCl3 and SFN co-treatment showed improvement in the motility, viability, and sperm count compared with the AlCl3 only group (p = 0.02). Furthermore, there was a significant decline in the levels of hormones profile and antioxidant status in AlCl3 only group compared to the control and SFN only (p = 0.02). The testicular histoarchitecture of the AlCl3 only group showed shrinkage of seminiferous tubules, spermatogenesis disruption, and empty lumen compared to the control and SFN only groups. Conclusion: The present study revealed the ameliorative response of SFN on AlCl3-induced testicular toxicity on serum hormone profiles, antioxidant status, lipid peroxidation, and histomorphometric analysis through oxidative stress. Key words: Sulforaphane, Aluminum trichloride, Oxidative stress, Testis, Histology.

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Kolaviron an Active Biflavonoid of Garcinia kola Extract Prevent 1,2-dimethylhydrazine Induced Oxidative Stress and Lipid Peroxidation in the Initiation Phase of Colon Carcinogenesis in Wistar Rats

Journal of Cancer and Tumor International ◽

10.9734/jcti/2015/16809 ◽

2015 ◽

Vol 2 (2) ◽

pp. 41-49

Author(s):

A. Eboh ◽

D. Ere ◽

L. Chuku ◽

A. Uwakwe

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Wistar Rats ◽

Colon Carcinogenesis ◽

Garcinia Kola ◽

Initiation Phase

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Prooxidant activity of norbixin in model of acute gastric ulcer induced by ethanol in rats

Human & Experimental Toxicology ◽

10.1177/0960327115604199 ◽

2015 ◽

Vol 35 (7) ◽

pp. 737-746 ◽

Cited By ~ 2

Author(s):

BT Rovani ◽

RB de Freitas ◽

PR Augusti ◽

IC Araldi ◽

S Somacal ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Gastric Ulcer ◽

Food Industry ◽

Wistar Rats ◽

Gastric Tissue ◽

Prooxidant Activity ◽

Prooxidant Effect ◽

Male Wistar Rats ◽

And Oxidative Stress

Free radicals and oxidative stress play a central role in gastric injuries caused by ethanol (EtOH). Antioxidant strategies to counteract EtOH toxicity are highly desirable. Norbixin (NBIX) is a carotenoid with antioxidant potential largely used in the food industry. This study evaluated the NBIX effects in a model of gastric ulcer induced by EtOH in rats. Male Wistar rats received NBIX doses of 0, 10, and 25 mg/kg by gavage 1 h after EtOH administration (0 or 75% solution, 1 mL/200 g of animal). The animals were euthanized 1 h after the NBIX administration, and their stomachs were removed for macroscopic and histopathological analyses, quantification of nonprotein sulfhydryl (NPSH) groups, lipid peroxidation (LPO) levels, and catalase (CAT) activity determination. NBIX increased LPO in gastric mucosa and caused CAT inhibition and NPSH depletion in EtOH-treated animals. Results showed that NBIX did not protect gastric tissue against EtOH damage, and this could be associated to a prooxidant effect.

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Aging Induces Hepatic Oxidative Stress and Nuclear Proteomic Remodeling in Liver from Wistar Rats

Antioxidants ◽

10.3390/antiox10101535 ◽

2021 ◽

Vol 10 (10) ◽

pp. 1535

Author(s):

Brenda Bárcena ◽

Aurora Salamanca ◽

Cristina Pintado ◽

Lorena Mazuecos ◽

Margarita Villar ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Rna Splicing ◽

Wistar Rats ◽

Prolonged Fasting ◽

Antioxidant Genes ◽

Male Wistar Rats ◽

Isobaric Tag ◽

Old Rats ◽

Nuclear Proteome

Aging is a continuous, universal, and irreversible process that determines progressive loss of adaptability. The liver is a critical organ that supports digestion, metabolism, immunity, detoxification, vitamin storage, and hormone signaling. Nevertheless, the relationship between aging and the development of liver diseases remains elusive. In fact, although prolonged fasting in adult rodents and humans delays the onset of the disease and increases longevity, whether prolonged fasting could exert adverse effects in old organisms remains incompletely understood. In this work, we aimed to characterize the oxidative stress and nuclear proteome in the liver of 3-month- and 24-month-old male Wistar rats upon 36 h of fasting and its adaptation in response to 30 min of refeeding. To this end, we analyzed the hepatic lipid peroxidation levels (TBARS) and the expression levels of genes associated with fat metabolism and oxidative stress during aging. In addition, to gain a better insight into the molecular and cellular processes that characterize the liver of old rats, the hepatic nuclear proteome was also evaluated by isobaric tag quantitation (iTRAQ) mass spectrometry-based proteomics. In old rats, aging combined with prolonged fasting had great impact on lipid peroxidation in the liver that was associated with a marked downregulation of antioxidant genes (Sod2, Fmo3, and Cyp2C11) compared to young rats. Besides, our proteomic study revealed that RNA splicing is the hepatic nuclear biological process markedly affected by aging and this modification persists upon refeeding. Our results suggest that aged-induced changes in the nuclear proteome could affect processes associated with the adaptative response to refeeding after prolonged fasting, such as those involved in the defense against oxidative stress.

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Effect of methanolic extract of Dennettia tripetala (pepper fruit) on biomarkers of oxidative stress and lipid peroxidation in type 2 diabetic male wistar rats

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2019.17028 ◽

2020 ◽

Vol 19 (2) ◽

pp. 99-105

Author(s):

Ogochukwu Nwaeme ◽

Victor Chibueze Ude ◽

Ndubuisi Augustine ◽

Patrick Ugwudike ◽

Francis Agu ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Wistar Rats ◽

Methanolic Extract ◽

Pepper Fruit ◽

Dennettia Tripetala ◽

Male Wistar Rats ◽

Type 2 Diabetic ◽

Biomarkers Of Oxidative Stress

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Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000654 ◽

2020 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Masoud Nasiri ◽

Saja Ahmadizad ◽

Mehdi Hedayati ◽

Tayebe Zarekar ◽

Mehdi Seydyousefi ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Wistar Rats ◽

Enzymatic Antioxidants ◽

Acute Response ◽

Total Antioxidant ◽

Male Wistar Rats ◽

Exercise Induced ◽

And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

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