scholarly journals Haplotype Analysis of Interleukin-8 Gene Polymorphisms in Chronic and Aggressive Periodontitis

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Petra Borilova Linhartova ◽  
Jan Vokurka ◽  
Hana Poskerova ◽  
Antonin Fassmann ◽  
Lydie Izakovicova Holla
Keyword(s):  
Gene Polymorphisms ◽  
Alveolar Bone ◽  
Bacterial Colonization ◽  
Bone Destruction ◽  
Aggressive Periodontitis ◽  
Interleukin 8 ◽  
Tissue Loss ◽  
Taqman Assay ◽  
Genotype Frequencies ◽  
Control Association

Objectives. Periodontitis is an inflammatory disease characterized by connective tissue loss and alveolar bone destruction. Interleukin-8 (IL8) is important in the regulation of the immune response. The aim of this study was to analyze four polymorphisms in theIL8gene in relation to chronic (CP) and aggressive (AgP) periodontitis.Methods. A total of 492 unrelated subjects were included in this case-control association study. Genomic DNA of 278 patients with CP, 58 patients with AgP, and 156 controls were genotyped, using the 5′ nuclease TaqMan assay, forIL8(rs4073, rs2227307, rs2227306, and rs2227532) gene polymorphisms. Subgingival bacterial colonization was investigated by the DNA-microarray detection kit in a subgroup of subjects(N=247).Results. Allele and genotype frequencies of all investigatedIL8polymorphisms were not significantly different between the subjects with CP and/or AgP and controls (P>0.05). Nevertheless, the A(−251)/T(+396)/T(+781) and T(−251)/G(+396)/C(+781) haplotypes were significantly less frequent in patients with CP (2.0% versus 5.1%,P<0.02, OR = 0.34, 95% CI: 0.15–0.78, resp., 2.0% versus 4.5%,P<0.05, OR = 0.41, 95% CI: 0.18–0.97) than in controls.Conclusions. Although none of the investigated SNPs in theIL8gene was individually associated with periodontitis, some haplotypes can be protective against CP in the Czech population.

scholarly journals Periodontal Manifestations in a Patient with Haim-Munk Syndrome

2010 ◽  
Vol 04 (03) ◽  
pp. 338-340
Author(s):  
Kamile Erciyas ◽  
Serhat Inaloz ◽  
A. Fuat Erciyas
Keyword(s):  
Autosomal Recessive ◽  
Alveolar Bone ◽  
Bone Destruction ◽  
Autosomal Recessive Disorder ◽  
Aggressive Periodontitis ◽  
Pes Planus ◽  
Rare Autosomal Recessive Disorder ◽  
Rare Anomaly ◽  
Palmoplantar Hyperkeratosis

Haim-Munk syndrome is an extremely rare autosomal recessive disorder characterized clinically by palmoplantar hyperkeratosis, aggressive periodontitis with severe alveolar bone destruction, onychogryphosis, pes planus, arachnodactyly, and acro-osteolysis. Consanguinity seems a notable prerequisite. The aim of this study was therefore to report one case of this syndrome and to focus on the periodontal manifestations, in order to attract the attention of dental clinicians to this rare anomaly. (Eur J Dent 2010;4:338-340)

scholarly journals Prevalence and characteristics of aggressive periodontitis

2011 ◽  
Vol 23 (2) ◽  
Author(s):  
Diatri Nariratih ◽  
Janti Rusjanti ◽  
Agus Susanto
Keyword(s):  
Periodontal Disease ◽  
Diagnostic Criteria ◽  
Alveolar Bone ◽  
Bone Destruction ◽  
Aggressive Periodontitis ◽  
Attachment Loss ◽  
Localized Aggressive Periodontitis ◽  
Low Prevalence ◽  
Age Range ◽  
Bilateral Destruction

Aggressive Periodontitis is one of periodontal disease that generally affects individuals less than 30 years old, with a rapid attachment loss and alveolar bone destruction. Special features of the disease are affected incisors and first molars with symmetrical bilateral destruction. The purpose of this study was to determine the prevalence and characteristics of Aggressive Periodontitis at Student Clinics, Faculty of Dentistry Padjadjaran University on May to July 2010. Based on accidentally sampling, 415 new patients at Dental and Oral Hospital, Faculty of Dentistry Padjadjaran University and Dental and Oral Polyclinic at RSUP dr. Hasan Sadikin Bandung participated in this study. Each subject filled out the questionnaire and did a clinical examination. Patients with attachment loss ≥ 4 mm were referred for radiographic and microbiologic examination to support the diagnosis. The results showed that there were 13 patients who have the diagnostic criteria of Aggressive Periodontitis. Nine patients were diagnosed as Localized Aggressive Periodontitis, and 4 patients as Generalized Aggressive Periodontitis. It is concluded that the prevalence of Aggressive Periodontitis was 3.13%, and indicated low prevalence. Characteristics of Localized Aggressive Periodontitis patients were female in 20-29 age range, whereas characteristics of Generalized Aggressive Periodontitis patients were male in 30-39 age range.

scholarly journals Impact of metronidazole and amoxicillin combination on matrix metalloproteinases-1 and tissue inhibitors of matrix metalloproteinases balance in generalized aggressive periodontitis

2015 ◽  
Vol 09 (01) ◽  
pp. 053-059 ◽  
Author(s):  
Emine Cifcibasi ◽  
Alpdogan Kantarci ◽  
Selim Badur ◽  
Halim Issever ◽  
Serdar Cintan
Keyword(s):  
Matrix Metalloproteinases ◽  
Alveolar Bone ◽  
Statistical Significance ◽  
Bone Destruction ◽  
Test Group ◽  
Aggressive Periodontitis ◽  
Control Group ◽  
Tissue Inhibitors ◽  
Clinical Healing ◽  
Concentration Levels

ABSTRACT Objective: Generalized aggressive periodontitis (GAgP) is a complex periodontal disease affecting the entire dentition with a rapid destruction of the periodontium and resulting in loss of teeth. We hypothesized that better clinical healing of adjunctive use of amoxicillin plus metronidazole combination may be related to the effect of this combination therapy to restore imbalance between matrix metalloproteinases (MMP) and their tissue inhibitors (TIMP) which is associated with connective tissue and alveolar bone destruction in patients with GAgP. Materials and Methods: Twenty-eight subjects diagnosed with GAgP were recruited. Patients were randomly assigned to test or control groups. MMP-1/TIMP-1 ratio was compared between groups receiving scaling and root planning (SRP) alone (control) or in combination with amoxicillin plus metronidazole (test). Clinical periodontal variables were measured. Gingival crevicular fluid samples were obtained and analyzed for MMP-1 and TIMP-1. Measurements were taken at baseline and repeated at 3 and 6 months after therapy. Results: Total MMP-1 levels were significantly decreased in both groups (P < 0.05) at 3 and 6 months. MMP-1 concentration levels showed a similar pattern to MMP-1 total levels decreasing significantly at 3 months (P < 0.05). TIMP-1 concentration levels increased in the test group throughout the study period, while the difference did not reach statistical significance (P > 0.05). TIMP-1/MMP-1 balance was restored in test group at 6 months significantly better than the control group (P < 0.05). Conclusion: The results of this study suggest that metronidazole and amoxicillin combination as an adjunct to SRP results in better clinical healing through restoring TIMP-1/MMP-1 balance.

scholarly journals Terapi Bedah Flep dan Cangkok Tulang pada Periodontitis Agresif di Regio Gigi Anterior Mandibula

2012 ◽  
Vol 19 (1) ◽  
pp. 67
Author(s):  
Levina Mulya ◽  
Sri Lelyati C Masulili
Keyword(s):  
Case Report ◽  
Bone Graft ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
Bone Destruction ◽  
Aggressive Periodontitis ◽  
Flap Surgery ◽  
Pocket Depth ◽  
Anterior Teeth ◽  
Hereditary Factors

Background: Aggressive periodentitis is a multifactorial disease that usuallyOccurs at a young age with the disease spreading fast and found the bacteria. Commonly aggressive periodontitis associated with hereditary factors and lack of immune system so as to reveal any family history with the same disease, and found savere alveolar bone destruction that may ultimately lead to tooth loss. Aggressive periodontitis and generalized aggressive periodontitis. The development of aggressive periodontitis is difficult to predict, so the mechanical therapy is not sufficient, and required antibiotic therapy or surgical therapy. Aim: the purpose of this case report is to explain the procedures and result of surgical treatment of the flap surgery with bone graft in anterior teeth of the patients with generalized aggressive periodontitis. Case report: Cases one and two with complaints of anterior teeth mobility. On clinical examination teeth mobility two and three degree, absolute pocket depth 4-6 mm. Radiograpic examination bone loss reached one third apical in all region. The diagnosis of both cases in generalized aggressive periodontitis. Treatment: After initial theraphy heve been evaluated, flap surgery with bone graft done in booth cases. Control evaluation after 6 month from surgery, in clinically reduced pocket depth 1-2m and tooth mobility, in radiographically increased bone height and bone fill. Conclusion: Flap surgery with bone graft in generalized aggressive periodontitis can assist periodontal regeneration.

Functional Gene Polymorphisms in Aggressive and Chronic Periodontitis

2005 ◽  
Vol 84 (12) ◽  
pp. 1149-1153 ◽  
Author(s):  
P.M. Brett ◽  
P. Zygogianni ◽  
G.S. Griffiths ◽  
M. Tomaz ◽  
M. Parkar ◽  
...  
Keyword(s):  
Chronic Disease ◽  
Environmental Factors ◽  
Chronic Periodontitis ◽  
Gene Polymorphisms ◽  
Aggressive Periodontitis ◽  
Healthy Controls ◽  
Genetic Associations ◽  
Functional Polymorphisms ◽  
Aggressive Disease ◽  
Genotype Frequencies

There is strong evidence that genetic as well as environmental factors affect the development of periodontitis, and some suggestion that aggressive and chronic forms of the disease share the same genetic predisposition. This study addresses the hypothesis that there are both shared and unique genetic associations in these forms of periodontitis. A sample of 51 patients with aggressive disease, 57 patients with chronic disease, and 100 healthy controls was recruited for this study. Ten functional polymorphisms in 7 candidate genes were genotyped. The results show statistically significant (p ≤ 0.05) differences between genotype frequencies in aggressive and controls (IL-1B +3954 & IL-6 −174); chronic and controls (IL-6 −174 & VDR −1056); chronic and aggressive periodontitis (IL-1A −889); and periodontitis as a whole and controls (VDR −1056, TLR-4 399 & IL-6 −174). These results suggest that there are in fact both shared and unique genetic associations in aggressive and chronic periodontitis.

scholarly journals Haplotype analysis of interleukin-8 gene polymorphisms in chronic and aggressive periodontitis

2013 ◽  
Vol 4 ◽  
Author(s):  
Borilova Linhartova Petra ◽  
Vokurka Jan ◽  
Poskerov� Hana ◽  
Fassmann Anton�n ◽  
Izakovicov� Holl� Lydie
Keyword(s):  
Gene Polymorphisms ◽  
Haplotype Analysis ◽  
Aggressive Periodontitis ◽  
Interleukin 8

Five genetic polymorphisms of cytochrome P450 enzymes in the Czech non-Roma and Czech Roma population samples

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Lucie Dlouhá ◽  
Věra Adámková ◽  
Lenka Šedová ◽  
Věra Olišarová ◽  
Jaroslav A. Hubáček ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Genetic Polymorphisms ◽  
Metabolic Pathways ◽  
Cytochromes P450 ◽  
Taqman Assay ◽  
Cytochrome P450 Enzymes ◽  
Roma Population ◽  
Czech Population ◽  
Genotype Frequencies ◽  
Pcr Rflp

AbstractObjectivesCytochromes P450 play a role in human drugs metabolic pathways and their genes are among the most variable in humans. The aim of this study was to analyze genotype frequencies of five common polymorphisms of cytochromes P450 in Roma/Gypsy and Czech (non-Roma) population samples with Czech origin.MethodsRoma/Gypsy (n=302) and Czech subjects (n=298) were genotyped for CYP1A2 (rs762551), CYP2A6 (rs4105144), CYP2B6 (rs3745274) and CYP2D6 (rs3892097; rs1065852) polymorphisms using PCR-RFLP or Taqman assay.ResultsWe found significant allelic/genotype differences between ethnics in three genes. For rs3745274 polymorphism, there was increased frequency of T allele carriers in Roma in comparison with Czech population (53.1 vs. 43.7%; p=0.02). For rs4105144 (CYP2A6) there was higher frequency of T allele carriers in Roma in comparison with Czech population (68.7 vs. 49.8%; p<0.0001). For rs3892097 (CYP2D6) there was more carriers of the A allele between Roma in comparison with Czech population (39.2 vs. 38.2%; p=0.048). Genotype/allelic frequencies of CYP2D6 (rs1065852) and CYP1A2 (rs762551) variants did not significantly differ between the ethnics.ConclusionsThere were significant differences in allelic/genotype frequencies of some, but not all cytochromes P450 polymorphisms between the Czech Roma/Gypsies and Czech non-Roma subjects.

scholarly journals TSP-1-1223 A/G Polymorphism as a Potential Predictor of the Recurrence Risk of Bladder Cancer in a Chinese Population

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Xiao Yang ◽  
Pengchao Li ◽  
Xuejian Yang ◽  
Chao Qin ◽  
Qiang Cao ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Chinese Population ◽  
Recurrence Risk ◽  
Taqman Assay ◽  
Control Subjects ◽  
Kaplan Meier ◽  
Genotype Frequencies ◽  
Healthy Control ◽  
First Recurrence ◽  
Cancer Tissues

Backgrounds. TSP-1 is a glycoprotein that functions in the biology of bladder cancer. We investigated the relationship between the distribution ofTSP-1-1223 A/G polymorphism (rs2169830) and the clinical characteristics of bladder cancer.Materials and Methods. TaqMan assay was performed to determine the genotype of 609 cases and 670 control subjects in a Chinese population. Logistic regression was used to assess the association between the polymorphism and the risk of bladder cancer. Quantitative real-time polymerase chain reaction was performed to determineTSP-1mRNA expression. Survival curves were generated using the Kaplan-Meier method.Results. No significant differences were detected in the genotype frequencies of healthy control subjects and patients with bladder cancer. By contrast, the time until the first recurrence differed significantly between genotypes (P=0.017). The expression ofTSP-1mRNA in bladder cancer tissues was lower in patients with an AG genotype than in those with an AA genotype. The lowest expression was observed in patients with a GG genotype.Conclusions. In conclusion,TSP-1-1223 A/G polymorphism may contribute to the recurrence of bladder cancer in Chinese population.

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