Significance of Glutathione Peroxidase 1 and Caudal-Related Homeodomain Transcription Factor in Human Gastric Adenocarcinoma

Aim. To investigate the expressions of glutathione peroxidase 1 (GPX1) and caudal-related homeodomain transcription factor (CDX2) in GAC and their correlation with clinicopathological features and tumor cell proliferation.Methods. The expressions of GPX1, CDX2, and Ki67 were immunohistochemically evaluated in 172 GAC specimens. The association of GPX1 and CDX2 with patient’s clinicopathological features and Ki67 positive rate was analyzed statistically.Results. In 172 cases of GAC, the expression of GPX1 was weaker than that in adjacent normal mucosa, and the expression of CDX2 was higher than that in adjacent normal mucosa. High expression GPX1 strong-expression was associated with differentiation, Lauren type, WHO type and extensive lymph node metastasis of GAC. High expression of CDX2 was associated with differentiation, Lauren type, WHO type, extensive lymph node metastasis, and TNM of GAC. Survival curves showed that expressions of GPX1 and CDX2 were factors of good outcome ( and .02, resp.). According to multivariate analysis, only lymph node metastasis, TNM stage, and CDX2 expression were independently associated with survival. In addition, a strong association of GPX1 expression was noted with Ki67 and CDX2.Conclusions. The expression of GPX1 and CDX2 may play a role in the carcinogenesis, differentiation, and progression of GAC, and CDX2 may be an independent prognostic factor.

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Upregulated CNTN1 is associated with lymph node metastasis and poor prognosis of colorectal cancer

Cancer Biomarkers ◽

10.3233/cbm-190981 ◽

2020 ◽

pp. 1-9

Author(s):

Guangyao Li ◽

Zhengjun Zhang ◽

Guochao Ge ◽

Ke Fang ◽

Jianyu Zhu

Keyword(s):

Colorectal Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Tumor Development ◽

Normal Mucosa ◽

Clinical Analysis ◽

Immunoglobulin Superfamily ◽

Node Metastasis ◽

Adjacent Normal Mucosa ◽

Potential Biomarker

BACKGROUND: Contactin1 (CNTN1), a member of the immunoglobulin superfamily, is known to correlate with tumor development and progression. Although recent studies have found that elevated CNTN1 has been demonstrated in some types of cancers, the expression and prognosis of CNTN1 in colorectal cancer (CRC) are unclear. Here, we aimed to determine the clinicopathological characteristics and prognostic role of CNTN1 in CRC patients. METHODS: The protein expression of CNTN1 in tumor tissues was evaluated by immunohistochemistry. In addition, the mRNA and protein expressions of CNTN1 were examined by qRT-PCR and Western blotting analysis in 40 matched adjacent normal mucosa samples. The relationships of CNTN1 with clinicopathological data and prognosis significance were analyzed. RESULTS: Immunohistochemical consequence suggested that the protein level of CNTN1 was obviously raised in CRC compared with adjacent normal mucosa tissues (56.9% vs 10.3%, P< 0.05). In addition, we detected a significant increase in CNTN1 mRNA and protein levels in CRC tissues compared with the matched adjacent normal mucosa tissues. Moreover, increased CNTN1 exprssion was significantly associated with tumor size, lymph node metastasis (LNM), tumor node-metastasis (TNM) stage and carcino-embryonic antigen (CEA) in clinical analysis. Kaplan-Meier analysis suggested that patients with CNTN1 over-expression showed worse overall survival (OS) (P= 0.001). Multivariate analysis indicated that high CNTN1 expression was an independent predictor for poor OS in CRC patients (P= 0.028). Further analysis revealed that patients with high CNTN1 combined with LNM present accurately predicted poorer outcome. CONCLUSION: Taken together, the findingsindicate that CNTN1 plays a significant role and serve as a potential biomarker for the prediction of adverse prognosis in CRC. Intriguingly, high express of CNTN1 + LNM-present combination may improve the accuracy of prognosis.

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Silencing of Glutathione Peroxidase 3 through DNA Hypermethylation Is Associated with Lymph Node Metastasis in Gastric Carcinomas

PLoS ONE ◽

10.1371/journal.pone.0046214 ◽

2012 ◽

Vol 7 (10) ◽

pp. e46214 ◽

Cited By ~ 36

Author(s):

Dun-Fa Peng ◽

Tian-Ling Hu ◽

Barbara G. Schneider ◽

Zheng Chen ◽

Ze-Kuan Xu ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Glutathione Peroxidase ◽

Dna Hypermethylation ◽

Gastric Carcinomas ◽

Node Metastasis ◽

Glutathione Peroxidase 3

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T-box transcription factor Brachyury expression is correlated with epithelial-mesenchymal transition and lymph node metastasis in oral squamous cell carcinoma

International Journal of Oncology ◽

10.3892/ijo.2012.1673 ◽

2012 ◽

Vol 41 (6) ◽

pp. 1985-1995 ◽

Cited By ~ 32

Author(s):

IKUMI IMAJYO ◽

TSUYOSHI SUGIURA ◽

YOSUKE KOBAYASHI ◽

MIYUKI SHIMODA ◽

KOTARO ISHII ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Transcription Factor ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Epithelial Mesenchymal Transition ◽

Mesenchymal Transition ◽

T Box ◽

Node Metastasis

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Clinicopathological and Prognostic Characteristics of Malaysian Triple Negative Breast Cancer Patients Undergoing TAC Chemotherapy Regimen

International Journal of Breast Cancer ◽

10.1155/2020/8424365 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Ahmad Aizat Abdul Aziz ◽

Md Salzihan Md Salleh ◽

Ravindran Ankathil

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Axillary Lymph Node ◽

Triple Negative ◽

Axillary Lymph Node Metastasis ◽

Clinicopathological Features ◽

Axillary Lymph ◽

Node Metastasis ◽

Positive Axillary Lymph Node ◽

Tac Chemotherapy

Triple negative breast cancer (TNBC) is associated with aggressive tumour phenotype and early tumour relapse following diagnosis. Generally, clinicopathological features such as tumour size, patient’s age at diagnosis, tumour histology subtypes, grade and stage, involvement of lymph nodes, and menopausal status are commonly used for predicting disease progression, prospects of recurrence, and treatment response. Prognostic value of clinicopathological features on Malaysian TNBC patients is limited. Thus, this study is aimed at investigating the association of clinicopathological features on disease-free survival (DFS) and overall survival (OS) of Malaysian TNBC patients undergoing TAC chemotherapy. Seventy-six (76) immunohistochemistry-confirmed TNBC patients were recruited. The clinicopathological features of TNBC patients were collected and recorded. Kaplan-Meier and log-rank followed by a Cox proportional hazard regression model were performed to evaluate the TNBC patients’ survival. Out of 76 TNBC patients, 25 were chemoresistant and 51 were chemoresponders to the TAC chemotherapy regimen. The overall 5-year cumulative DFS and OS of TNBC patients were 63.5% and 76.3%, respectively. Multivariate Cox analysis demonstrated that medullary and metaplastic histology subtypes and positive axillary lymph node metastasis were significant prognostic factors associated with relapse with adjusted HR: 5.76, 95% CI: 2.35, 14.08 and adjusted HR: 3.55, 95% CI: 1.44, 8.74, respectively. Moreover, TNBC patients with medullary and metaplastic histology subtypes and positive axillary lymph node metastases had a higher risk to death than patients who had infiltrating ductal carcinoma and negative axillary lymph node metastasis (adjusted HR: 8.30, 95% CI: 2.38, 28.96 and adjusted HR: 6.12, 95% CI: 1.32, 28.42, respectively). Our results demonstrate the potential use of medullary and metaplastic histology subtype and positive axillary lymph node metastasis as a potential biomarker in predicting relapse and survival of the TNBC patients. This warrants further studies on intensification of chemotherapy and also identification and development of targeted therapy to reduce relapses and improve survival of TNBC patients.

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Proteomic analysis shows down-regulations of cytoplasmic carbonic anhydrases, CAI and CAII, are early events of colorectal carcinogenesis but are not correlated with lymph node metastasis

Tumori Journal ◽

10.1177/030089161209800617 ◽

2012 ◽

Vol 98 (6) ◽

pp. 783-791 ◽

Cited By ~ 4

Author(s):

Ning Wang ◽

Yang Chen ◽

Yuchen Han ◽

Yue Zhao ◽

Yu Liu ◽

...

Keyword(s):

Colorectal Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Cancer Progression ◽

Normal Mucosa ◽

Laser Desorption Ionization ◽

Ionization Time ◽

Node Metastasis ◽

Flight Mass Spectrometry ◽

Differential Gel Electrophoresis

Aim The aim of the study was to screen the markedly down-regulated proteins in colorectal cancer and analyze their relationship to carcinogenesis, cancer progression and pathological aspects. Methods Proteomic analysis was preformed on six fresh colorectal cancer tissues and paired normal colorectal mucosa by two-dimensional differential gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Two markedly down-regulated proteins among the proteins, of which the expressions were significantly decreased in colorectal cancer compared to normal mucosa, were confirmed by Western Blot in 12 colorectal cancers. Their relationship to carcinogenesis, cancer progression and pathological aspects of colorectal cancer were analyzed in 64 colorectal cancer and paired normal mucosa, 27 benign polyps, and 20 lymph node metastases by immunohistochemistry. Results Two-dimensional differential gel electrophoresis analysis showed there were 2 protein spots, of which the average abundances decreased 3.62 and 3.76 fold in colorectal cancer compared to normal mucosa, respectively. They were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry as carbonic anhydrase I and II (CAI and CAII). Validation by Western Blot in 12 colorectal cancers showed there were significantly different expressions of CAI and CAII between colorectal cancer and normal mucosa (P = 0.002 and 0.027, respectively). Immunohistochemistry analysis indicated the expression of CAI and CAII was decreased from normal mucosa to benign polyps, and to colorectal cancer stepwise significantly (P <0.05). However, there were no differences in their expressions between lymph node metastasis and colorectal cancer (P >0.05). There were decreasing trends of CAI and CAII expressions from well to poor differentiation and from stage I or II to stage III or IV, but they were not statistically significant (P >0.05). Conclusions CAI and CAII are necessary enzymes of the colorectum for their normal function. Down-regulations of CAI and CAII are early events of colorectum carcinogenesis. They have no correlations with lymph node metastasis.

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A high expression level of insulin-like growth factor I receptor is associated with increased expression of transcription factor Sp1 and regional lymph node metastasis of human gastric cancer

Clinical & Experimental Metastasis ◽

10.1007/s10585-005-1198-2 ◽

2005 ◽

Vol 21 (8) ◽

pp. 755-764 ◽

Cited By ~ 54

Author(s):

Yixing Jiang ◽

Liwei Wang ◽

Weida Gong ◽

Daoyan Wei ◽

Xiangdong Le ◽

...

Keyword(s):

Gastric Cancer ◽

Transcription Factor ◽

Lymph Node ◽

Growth Factor ◽

Lymph Node Metastasis ◽

Regional Lymph Node ◽

Human Gastric Cancer ◽

Factor I ◽

Node Metastasis ◽

High Expression Level

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A case of invasive micropapillary carcinoma of the breast involving extensive lymph node metastasis

World Journal of Surgical Oncology ◽

10.1186/1477-7819-12-84 ◽

2014 ◽

Vol 12 (1) ◽

pp. 84 ◽

Cited By ~ 3

Author(s):

Kenji Taketani ◽

Eriko Tokunaga ◽

Nami Yamashita ◽

Kimihiro Tanaka ◽

Yoko Zaitsu ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Invasive Micropapillary Carcinoma ◽

Micropapillary Carcinoma ◽

Carcinoma Of The Breast ◽

Node Metastasis ◽

Extensive Lymph Node

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The T-box transcription factor eomesodermin controls CD8 T cell activity and lymph node metastasis in human colorectal cancer

Gut ◽

10.1136/gut.2006.117812 ◽

2007 ◽

Vol 56 (11) ◽

pp. 1572-1578 ◽

Cited By ~ 36

Author(s):

I. Atreya ◽

C. C Schimanski ◽

C. Becker ◽

S. Wirtz ◽

H. Dornhoff ◽

...

Keyword(s):

Colorectal Cancer ◽

Transcription Factor ◽

Lymph Node ◽

T Cell ◽

Lymph Node Metastasis ◽

Cell Activity ◽

Cd8 T Cell ◽

Human Colorectal Cancer ◽

T Box ◽

Node Metastasis

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Effect of a mixed type Lauren's classification on agressiveness and lymph node metastasis in early gastric cancer.

Journal of Clinical Oncology ◽

10.1200/jco.2017.35.4_suppl.28 ◽

2017 ◽

Vol 35 (4_suppl) ◽

pp. 28-28

Author(s):

Jeunghui Pyo ◽

Hyuk Lee ◽

In Seub Shin ◽

Tae Jun Kim ◽

Yang Won Min ◽

...

Keyword(s):

Gastric Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Early Gastric Cancer ◽

Mixed Type ◽

Clinicopathological Features ◽

Node Metastasis ◽

Lauren's Classification ◽

Lauren’S Classification

28 Background: The clinicopathological features of mixed type (MT) early gastric cancer (EGC) according to Lauren’s classification remain uninvestigated. This study aimed to clarify the clinicopathological features of MT EGC, particularly in relation to lymph node metastasis (LNM) and long-term survival. Methods: This study included 5,309 patients who underwent gastrectomy for EGC. The clinicopathological features, LNM, and long-term outcomes of patients with MT carcinomas were compared to those with intestinal type (IT) and diffuse type (DT) cancers. Furthermore, we evaluated the predictors of LNM in each Lauren classification subgroup. Results: Patients with MT carcinomas were more likely to have larger tumors, submucosal invasion, lymphovascular invasion (LVI), and LNM compared to those with IT or DT carcinomas. Multivariate logistic regression analysis revealed that Lauren’s classification was a significant predictor of LNM ( P < 0.001). The significant predictors of LNM in MT carcinomas were female sex, greater tumor size, presence of submucosal invasion, and LVI. However, the overall survival of patients with MT carcinoma was not significantly different from that of patients with IT or DT carcinomas ( P= 0.104). Conclusions: The presence of MT EGC carries a higher risk of LNM compared to IT or DT carcinomas. Therefore, MT carcinomas should be managed with gastrectomy that includes lymph node dissection instead of endoscopic resection.

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