scholarly journals Platelet-Rich Fibrin Promotes Periodontal Regeneration and Enhances Alveolar Bone Augmentation

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Qi Li ◽  
Shuang Pan ◽  
Smit J. Dangaria ◽  
Gokul Gopinathan ◽  
Antonia Kolokythas ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
Principal Component ◽  
Nodule Formation ◽  
Bone Augmentation ◽  
Pilot Studies ◽  
Platelet Rich Fibrin ◽  
Lineage Differentiation ◽  
Osteogenic Lineage

In the present study we have determined the suitability of platelet-rich fibrin (PRF) as a complex scaffold for periodontal tissue regeneration. Replacing PRF with its major component fibrin increased mineralization in alveolar bone progenitors when compared to periodontal progenitors, suggesting that fibrin played a substantial role in PRF-induced osteogenic lineage differentiation. Moreover, there was a 3.6-fold increase in the early osteoblast transcription factor RUNX2 and a 3.1-fold reduction of the mineralization inhibitor MGP as a result of PRF application in alveolar bone progenitors, a trend not observed in periodontal progenitors. Subcutaneous implantation studies revealed that PRF readily integrated with surrounding tissues and was partially replaced with collagen fibers 2 weeks after implantation. Finally, clinical pilot studies in human patients documented an approximately 5 mm elevation of alveolar bone height in tandem with oral mucosal wound healing. Together, these studies suggest that PRF enhances osteogenic lineage differentiation of alveolar bone progenitors more than of periodontal progenitors by augmenting osteoblast differentiation, RUNX2 expression, and mineralized nodule formation via its principal component fibrin. They also document that PRF functions as a complex regenerative scaffold promoting both tissue-specific alveolar bone augmentation and surrounding periodontal soft tissue regeneration via progenitor-specific mechanisms.

scholarly journals Effect of advanced platelet-rich fibrin applications on periodontal regeneration in infrabony pocket treatment

2019 ◽  
Vol 4 (3) ◽  
pp. 154
Author(s):  
Christopher Imantaka Suwondo ◽  
Dahlia Herawati ◽  
Sudibyo Sudibyo
Keyword(s):  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
X Rays ◽  
Periodontal Tissue ◽  
Platelet Rich Fibrin ◽  
Attachment Loss ◽  
Probing Depth ◽  
Periodontal Tissue Regeneration ◽  
Bone Height

One of the regenerative periodontal treatments for infrabony pocket is open flap debridement (OFD) with the addition of growth factor derived from platelet concentrate. Advanced platelet-rich fibrin (A-PRF) is a further development of plateletrich fibrin (PRF) with a lower centrifugation speed (1,500 rpm, 14 minutes). The purpose of this study was to examine the differences in periodontal tissue regeneration after the application of A-PRF and PRF in the treatment of infrabony pockets evaluated from probing depth (PD), relative attachment loss (RAL), and alveolar bone height. The samples were taken from 20 infrabony pockets divided into 2 groups: 10 subjects were treated with OFD+A-PRF and OFD+PRF on the remaining subjects. Probing depth (PD) and relative attachment loss (RAL) measurement were performed on days 0, 30, and 90. Bone height measurements were performed using CBCT X-rays on days 0 and 90. The results showed that PD and RAL reduction in the group of OFD+A-PRF was significantly greater than that in the OFD+PRF group. Bone height reduction in both groups showed no difference. The conclusion obtained from this study is A-PRF application enhances periodontal tissue regeneration by generating greater probing depth and relative attachment loss reduction compared toPRF, as well as an increase in bone height similar to in the treatment of infrabony pockets.

scholarly journals Erratum to “Platelet-Rich Fibrin Promotes Periodontal Regeneration and Enhances Alveolar Bone Augmentation”

2020 ◽  
Vol 2020 ◽  
pp. 1-1
Author(s):  
Qi Li ◽  
Shuang Pan ◽  
Smit J. Dangaria ◽  
Gokul Gopinathan ◽  
Antonia Kolokythas ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
Bone Augmentation ◽  
Platelet Rich Fibrin

scholarly journals Clinical and Histologic Evaluation of Using Block Xenograft Combined With Leukocyte-Platelet Rich Fibrin (L-PRF) Versus Intraoral Autogenous Bone Block Graft With L-PRF in Treating Localized Ridge Defects: A Randomized Clinical Trial

Perio J ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 11-21
Author(s):  
Ahmed Y. Gamal ◽  
Shahinaz G. Elashiry ◽  
Fatma H. Eldemerdash ◽  
Omar M. Elnashar
Keyword(s):  
Alveolar Bone ◽  
Autogenous Bone ◽  
Bone Block ◽  
Bone Augmentation ◽  
Class Iii ◽  
Platelet Rich Fibrin ◽  
Oral Implants ◽  
Histologic Evaluation

Background: Augmentation of vertical bone defects remains the corner stone in periodontal tissue engineering. The amount and quality of alveolar bone available in all dimensions affects the success of dental implants for restoration of edentulous areas. Adequate and healthy bone supports the degree of osseointegration which in turn affects the long-term success of oral implants. The primary aim of the study was to histologically evaluate autogenous block grafts versus synthetic block grafts for the treatment of atrophic vertical and horizontal bony defects (Siebert Class III) in the anterior esthetic zone of the mouth. The secondary aim was to clinically and radiographically evaluate the outcomes of the procedure. Methods: This was a randomized controlled clinical study with a statistically determined sample size of 10 patients per group and a total of 20 patients in both groups. Patients with vertical and horizontal bone loss were enrolled from the Department of Oral Medicine, Periodontology, and Oral Diagnosis of Ain Shams University and Misr International University. Bone augmentation procedures were performed using two techniques: autogenous bone block graft and xenograft bone block graft both with leukocyte-platelet rich fibrin (L-PRF). Results: Both autogenous and xenograft blocks in conjunction with L-PRF had a significant effect on vertical bone augmentation in cases of atrophic ridges in the esthetic region. Conclusion: Both autogenous and xenograft bone blocks in conjunction with L-PRF have a significant effect on vertical bone augmentation in cases of atrophic ridges in the esthetic region.

scholarly journals Periradicular tissue necrosis caused by accidentally injected sodium hypochlorite through fausse route in upper maxillary canine

2020 ◽  
Vol 67 (4) ◽  
pp. 219-224
Author(s):  
Djordje Pejanovic ◽  
Milana Cabrilo ◽  
Dusan Djuric ◽  
Vladimir Biocanin
Keyword(s):  
Sodium Hypochlorite ◽  
Tissue Regeneration ◽  
Soft Tissues ◽  
Bone Augmentation ◽  
Tissue Necrosis ◽  
Dental Clinic ◽  
Maxillary Canine ◽  
Bone Necrosis ◽  
Platelet Rich Fibrin ◽  
Antiseptic Agent

Sodium hypochlorite (NaOCl) is a strong antiseptic agent that is usually used as endodontic irrigant for dissolving organic parts of pulp and dentin and neutralizing toxic products. However, the use of sodium hypochlorite may cause destruction of blood vessels, soft tissues and bone necrosis if extruded into the periapical region. Urgent treatment of the NaOCl accident should be oriented on pain relief, infection control and recovery. This paper shows a case of periradicular tissue necrosis after accidental extrusion on NaOCl through fausse route of right maxillary canine, and complete tissue regeneration after surgical debridement and bone augmentation with addition of platelet rich fibrin (PRF) that occurred in a 53-year old patient at the dental clinic of Faculty of Dentistry in Pancevo.

The Application of Three-Dimensional Printing Model and Platelet-Rich Fibrin Technology in Guided Tissue Regeneration Surgery for Severe Bone Defects

2019 ◽  
Vol 45 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Lihong Lei ◽  
Yuanyuan Yu ◽  
Ting Ke ◽  
Weilian Sun ◽  
Lili Chen
Keyword(s):  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Three Dimensional ◽  
Guided Tissue Regeneration ◽  
Collagen Membrane ◽  
Three Dimensional Printing ◽  
Platelet Rich Fibrin ◽  
Bony Anatomy ◽  
Regenerative Periodontal Therapy ◽  
Radiographic Followup

A 36-year-old male patient diagnosed with severe chronic periodontitis was treated with novel surgery for his maxillary right lateral incisor. Preoperatively, a 3D printer was used, based on CBCT datasets, to produce a photosensitive resin bony anatomy replica. The patient's blood was centrifuged to obtain advanced platelet-rich fibrin (A-PRF) and injected platelet-rich fibrin (I-PRF), then mixed with Bio-Oss and packed onto the 3D replica to form the ideal shape. The replica was positioned at the planned sites without changes. The A-PRF membrane was applied over the replica as well as a Bio-Gide collagen membrane. Fifteen months after the surgery, clinical and radiographic followup revealed greatly reduced pocket depths and significant 3D alveolar bone fill at the treatment site. Based on these short-term results, the initial 3D printing surgical temple assisted guided tissue regeneration method resulted in significant clinical and radiographic improvements; A-PRF/I-PRF should be considered an ideal biomaterial for regenerative periodontal therapy.

scholarly journals Tooth-Supporting Hard Tissue Regeneration Using Biopolymeric Material Fabrication Strategies

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4802
Author(s):  
Min Guk Kim ◽  
Chan Ho Park
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Critical Role ◽  
Periodontal Regeneration ◽  
Tissue Formation ◽  
Hard Tissue ◽  
Periodontal Tissues ◽  
Mineralized Tissues ◽  
Biomechanical Forces

The mineralized tissues (alveolar bone and cementum) are the major components of periodontal tissues and play a critical role to anchor periodontal ligament (PDL) to tooth-root surfaces. The integrated multiple tissues could generate biological or physiological responses to transmitted biomechanical forces by mastication or occlusion. However, due to periodontitis or traumatic injuries, affect destruction or progressive damage of periodontal hard tissues including PDL could be affected and consequently lead to tooth loss. Conventional tissue engineering approaches have been developed to regenerate or repair periodontium but, engineered periodontal tissue formation is still challenging because there are still limitations to control spatial compartmentalization for individual tissues and provide optimal 3D constructs for tooth-supporting tissue regeneration and maturation. Here, we present the recently developed strategies to induce osteogenesis and cementogenesis by the fabrication of 3D architectures or the chemical modifications of biopolymeric materials. These techniques in tooth-supporting hard tissue engineering are highly promising to promote the periodontal regeneration and advance the interfacial tissue formation for tissue integrations of PDL fibrous connective tissue bundles (alveolar bone-to-PDL or PDL-to-cementum) for functioning restorations of the periodontal complex.

scholarly journals Guided Tissue Regeneration – A Boon to Surgical Periodontal Therapy

2020 ◽  
Vol 5 (7) ◽  
pp. 471-474
Author(s):  
Dr. Akhilesh Sankhyayan ◽  
Dr. Anil Sharma ◽  
Vidushi Jindal ◽  
Dr. Malvika Thakur ◽  
Dr. Vikas Jindal ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Inflammatory Disease ◽  
Periodontal Ligament ◽  
Calcium Sulfate ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
Chronic Inflammatory Disease ◽  
Guided Tissue Regeneration ◽  
Periodontal Pocket ◽  
Pocket Formation

Periodontitis has been a chronic inflammatory disease of the gingiva which eventually result in periodontal pocket formation with loss of the associated periodontal ligament and alveolar bone around teeth. Guided tissue regeneration (GTR), which is often a target for periodontal treatment, has the ability to promote periodontal regeneration. The development of the periodontal attachment is primarily concerned with tissue regeneration.Based on such concept, guided tissue regeneration is being utilized to varying degree of success to restore periodontal defects. In order to remove epithelium as well as gingival corium from the root and/or existing bone walls on the assumption that they interfere with regeneration, barrier techniques have been applied, using elements like expanded polytetrafluoroethylene, polyglactine, polylactic acid, calcium sulfate and collagen.

scholarly journals Application of a BMP2-binding heparan sulphate to promote periodontal regeneration

2021 ◽  
Vol 42 ◽  
pp. 139-153
Author(s):  
BQ Le ◽  
◽  
JH Too ◽  
TC Tan ◽  
RAA Smith ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Heparan Sulphate ◽  
Periodontal Regeneration ◽  
Bone Morphogenetic Protein 2 ◽  
Bone Tissue Regeneration ◽  
Tissue Loss ◽  
Periodontal Tissues ◽  
Combination Device

Periodontitis is the most common inflammatory disease that leads to periodontal defects and tooth loss. Regeneration of alveolar bone and soft tissue in periodontal defects is highly desirable but remains challenging. A heparan sulphate variant (HS3) with enhanced affinity for bone morphogenetic protein-2 (BMP2) that, when combined with collagen or ceramic biomaterials, enhances bone tissue regeneration in the axial and cranial skeleton in several animal models was reported previously. In the current study, establishing the efficacy of a collagen/HS3 device for the regeneration of alveolar bone and the adjacent periodontal apparatus and related structures was sought. Collagen sponges loaded with phosphate-buffered saline, HS3, BMP2, or HS3 + BMP2 were implanted into surgically-created intra-bony periodontal defects in rat maxillae. At the 6 week end- point the maxillae were decalcified, and the extent of tissue regeneration determined by histomorphometrical analysis. The combination of collagen/HS3, collagen/BMP2 or collagen/HS3 + BMP2 resulted in a three to four-fold increase in bone regeneration and up to a 1.5 × improvement in functional ligament restoration compared to collagen alone. Moreover, the combination of collagen/HS3 + BMP2 improved the alveolar bone height and reduced the amount of epithelial growth in the apical direction. The implantation of a collagen/ HS3 combination device enhanced the regeneration of alveolar bone and associated periodontal tissues at amounts comparable to collagen in combination with the osteogenic factor BMP2. This study highlights the efficacy of a collagen/HS3 combination device for periodontal regeneration that warrants further development as a point-of-care treatment for periodontitis-related bone and soft tissue loss.

Regeneration of Periodontal Tissues in Non-human Primates with rhGDF-5 and Beta-Tricalcium Phosphate

2011 ◽  
Vol 90 (12) ◽  
pp. 1416-1421 ◽  
Author(s):  
K.B. Emerton ◽  
S.J. Drapeau ◽  
H. Prasad ◽  
M. Rohrer ◽  
P. Roffe ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Tricalcium Phosphate ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
Human Growth ◽  
Graft Material ◽  
Periodontal Tissue ◽  
Periodontal Tissues ◽  
Beta Tricalcium Phosphate ◽  
Periodontal Tissue Regeneration

The application of growth factors has been advocated in support of periodontal regeneration. Recombinant human growth and differentiation factor-5 (rhGDF-5), a member of the bone morphogenetic protein family, has been used to encourage periodontal tissue regeneration. This study evaluated the dose response of rhGDF-5 lyophilized onto beta-tricalcium phosphate (bTCP) granules for periodontal tissue regeneration in a baboon model. Periodontal defects were created bilaterally in 12 baboons by a split-mouth design. Plaque was allowed to accumulate around wire ligatures to create chronic disease. After 2 mos, the ligatures were removed, and a notch was placed at the base of the defect. Two teeth on each side of the mouth were randomly treated with bTCP only, 0.5, 1.0, or 2.0 mg rhGDF-5/g bTCP. Animals were sacrificed 5 mos post-treatment, with micro-CT and histomorphometric analysis performed. After 5 mos, analysis showed alveolar bone, cementum, and periodontal ligament formation in all treatment groups, with a dose-dependent increase in rhGDF-5-treated groups. Height of periodontal tissues also increased with the addition of rhGDF-5, and the amount of residual graft material decreased with rhGDF-5 treatment. Therefore, rhGDF-5 delivered on bTCP demonstrated effective regeneration of all 3 tissues critical for periodontal repair.

scholarly journals Platelet Rich Fibrin - A Saviour for Replanted Teeth – A Review

2021 ◽  
Vol 10 (33) ◽  
pp. 2816-2823
Author(s):  
Sanjana Mall ◽  
Rajmohan Shetty ◽  
Amitha Hegde ◽  
Kavita Rai
Keyword(s):  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Treatment Success ◽  
Periodontal Regeneration ◽  
Root Surface ◽  
Periodontal Ligament Cells ◽  
Platelet Concentrates ◽  
Soft Tissue Healing ◽  
Platelet Rich Fibrin ◽  
Tissue Healing

The periodontal ligament is a unique specialised connective tissue between the cementum covering the tooth root and the alveolar bone. It is believed that periodontal ligament cells are responsible for not only osteogenesis and osteoclasia of the alveolar bone but also for fibrogenesis and fibroplasia of the ligament itself, as well as cementogenesis and the presence of cementoblasts on the root surface. Injury to the periodontal ligament (PDL) and its compromised healing (external inflammatory resorption and replacement resorption) has been cited as one of the major reasons for the failure of transplantation and replantation procedures. The necessity of having a healthy PDL so that the tooth can re-attach and be retained in the socket determines the prognosis of replanted teeth. Thus, the importance of maintaining the periodontal viability has led to an increased interest in the development of platelet concentrates, which have been considered as autologous biomaterials having the ability to potentiate healing, repair, and regenerate. PRF (platelet rich fibrin) is a living biomaterial derived from human blood containing fibrin, platelets, growth factors, leukocytes and stem cells entrapped in a fibrin-based scaffold / matrix, which has been documented to promote bone and soft tissue healing and regeneration. PRF technology has grabbed the attention of clinicians because it is readily available, is easy to prepare, can be produced immediately at the chairside, is easy to use, and widely applicable in dentistry, while being financially realistic for the patient and the clinician. Thus, the purpose of this review is to enumerate the biologic, chemical and physical properties of PRF and highlight the essential role it plays in periodontal regeneration and repair, which can be highly beneficial in improving the treatment success rate of transplantation and replantation procedures. KEY WORDS Platelet Rich Fibrin, Replantation, Transplantation, Periodontal Ligament, Periodontal Regeneration

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