Is Delayed Pressure Urticaria Associated with Increased Systemic Release of sCD40L?

Background. Elevated levels of soluble CD40 Ligand (sCD40L) were found in serum but not in plasma of patients with chronic spontaneous urticaria (CU). What is important is that sCD40L has proinflammatory properties, and its elevated plasma level may indicate increased risk of cardiovascular events. These observations should stimulate further evaluation of sCD40L in different forms of urticaria.Aim. In the present study, sCD40L plasma level was investigated in delayed pressure urticaria (DPU).Methods. As platelets are predominant and variable sources of sCD40L, we investigated sCD40L concentration in platelet-poor plasma (PPP), which seems the best way to minimize the potential contribution of these cells to the ligand level.Results. Plasma sCD40L concentration was significantly increased in the DPU group compared to the healthy controls.Conclusions. It seems that DPU is associated with increased systemic release of sCD40L, which is believed to derive predominantly from activated platelets. The present study as well as the earlier contributions suggest that distinct cells activity, including platelets, may be identified in different types of urticaria.

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Activated platelets are the source of elevated levels of soluble CD40 ligand in the circulation of inflammatory bowel disease patients

Gut ◽

10.1136/gut.52.10.1435 ◽

2003 ◽

Vol 52 (10) ◽

pp. 1435-1441 ◽

Cited By ~ 156

Author(s):

S Danese

Keyword(s):

Inflammatory Bowel Disease ◽

Bowel Disease ◽

Cd40 Ligand ◽

Soluble Cd40 Ligand ◽

Activated Platelets ◽

Inflammatory Bowel

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Polycystic ovary syndrome is associated with elevated plasma soluble CD40 ligand, a marker of coronary artery disease

Fertility and Sterility ◽

10.1016/j.fertnstert.2008.03.039 ◽

2009 ◽

Vol 91 (6) ◽

pp. 2545-2550 ◽

Cited By ~ 15

Author(s):

Mesut Oktem ◽

Emel Ebru Ozcimen ◽

Ayla Uckuyu ◽

Ibrahim Esinler ◽

Baris Pamuk ◽

...

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Cd40 Ligand ◽

Elevated Plasma ◽

Ovary Syndrome ◽

Soluble Cd40 Ligand ◽

Artery Disease

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Preliminary evidence for a matrix metalloproteinase-2 (MMP-2)-dependent shedding of soluble CD40 ligand (sCD40L) from activated platelets

Platelets ◽

10.1080/09537100903096684 ◽

2009 ◽

Vol 20 (6) ◽

pp. 441-444 ◽

Cited By ~ 28

Author(s):

Stephan Reinboldt ◽

Folker Wenzel ◽

Bernhard H. Rauch ◽

Thomas Hohlfeld ◽

Maria Grandoch ◽

...

Keyword(s):

Matrix Metalloproteinase ◽

Cd40 Ligand ◽

Preliminary Evidence ◽

Matrix Metalloproteinase 2 ◽

Soluble Cd40 Ligand ◽

Activated Platelets

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Elevated Plasma Levels of the Atherogenic Mediator Soluble CD40 Ligand in Diabetic Patients

Circulation ◽

10.1161/01.cir.0000074043.46437.44 ◽

2003 ◽

Vol 107 (21) ◽

pp. 2664-2669 ◽

Cited By ~ 149

Author(s):

Nerea Varo ◽

David Vicent ◽

Peter Libby ◽

Rebecca Nuzzo ◽

Alfonso L. Calle-Pascual ◽

...

Keyword(s):

Plasma Levels ◽

Cd40 Ligand ◽

Diabetic Patients ◽

Elevated Plasma ◽

Soluble Cd40 Ligand

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Soluble CD40 Iigand and Oxidative Response Are Reciprocally Stimulated during Shiga Toxin-Associated Hemolytic Uremic Syndrome

10.20944/preprints201708.0064.v1 ◽

2017 ◽

Author(s):

Maria J Abrey Recalde ◽

Romina S. Alvarez ◽

Fabiana Alberto ◽

Maria p Mejias ◽

Maria V. Ramos ◽

...

Keyword(s):

Hemolytic Uremic Syndrome ◽

Shiga Toxin ◽

Thrombus Formation ◽

Cd40 Ligand ◽

Endothelial Damage ◽

Dependent Manner ◽

Soluble Cd40 Ligand ◽

Activated Platelets ◽

Oxidative Response ◽

Uremic Syndrome

Shiga toxin (Stx) produced by Escherichia coli is the main pathogenic factor of diarrhea-associated hemolytic uremic syndrome (HUS), which is characterized by obstruction of renal microvasculature by platelet-fibrin thrombi. It is well known that the oxidative imbalance generated by Stx induces platelet activation, contributing to thrombus formation. Moreover, activated platelets release soluble CD40 ligand (sCD40L) which in turn contributes to oxidative imbalance, triggering the release of reactive oxidative species (ROS) on various cellular types. The aim of this work was to determine if the interaction between the oxidative response and platelet-derived sCD40L participates in the pathogenic mechanism during HUS. Activated human glomerular endothelial cells (HGEC) by Stx2 induced platelets to adhere to them. Although platelet adhesion did not contribute to endothelial damage, high levels of sCD40L were released to the medium. The release of sCD40L by activated platelets was inhibited by antioxidant treatment. Furthermore, we found increased levels of sCD40L in plasma from HUS patients, which were also able to trigger the respiratory burst in monocytes, in a sCD40L-dependent manner. Thus, we concluded that platelet-derived sCD40L and the oxidative response are reciprocally stimulated during Stx2-associated HUS. This process may contribute to the evolution of glomerular occlusion and the microangiopathic lesions.

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Soluble CD40 Ligand Levels in Otherwise Healthy Subjects With Impaired Fasting Glucose

Mediators of Inflammation ◽

10.1155/mi/2006/32508 ◽

2006 ◽

Vol 2006 ◽

pp. 1-5

Author(s):

Ilker Tasci ◽

Teoman Dogru ◽

Alper Sonmez ◽

Halil Genc ◽

Selim Kilic ◽

...

Keyword(s):

Impaired Fasting Glucose ◽

Fasting Glucose ◽

Hdl Cholesterol ◽

Cd40 Ligand ◽

Soluble Cd40 Ligand ◽

Ligand Interaction ◽

Reactive Protein ◽

Increased Risk ◽

Soluble Cd40l ◽

And Control

Unlike diabetes mellitus and impaired glucose tolerance, it is not clear whether the subjects with impaired fasting glucose (IFG) are at increased risk of atherosclerosis and cardiovascular diseases. The CD40-CD40 ligand interaction is involved in the mechanism of atherosclerosis. We investigated whether soluble CD40L (sCD40L) as well as high sensitive C-reactive protein (hsCRP) levels are increased in subjects with IFG having no confounding factors for inflammation or atherosclerosis. Twenty four IFG subjects with no additional disorders and 40 appropriate healthy controls were studied. sCD40L and hsCRP levels in the IFG and control groups were similar. Blood pressures, total and LDL-cholesterol, and triglyceride levels were also similar, whereas HDL-cholesterol was lower and HOMA-IR indexes were higher in the IFG group. Though the sample size was small, the present data show that sCD40L seems not to alter in subjects with IFG suggesting that it might not be an independent risk factor for atherosclerosis.

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Persistently higher serum sCD40L levels are associated with outcome in septic patients

BMC Anesthesiology ◽

10.1186/s12871-021-01241-9 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Yingjian Liang ◽

Chengrui Zhu ◽

Yini Sun ◽

Zhiliang Li ◽

Liang Wang ◽

...

Keyword(s):

Intensive Care Unit ◽

Septic Shock ◽

Odds Ratio ◽

Cd40 Ligand ◽

Surgical Patients ◽

Soluble Cd40 Ligand ◽

Icu Admission ◽

Time Changes ◽

And Control ◽

Type Of Surgery

Abstract Background Soluble CD40 ligand (sCD40L) exhibits proinflammatory and procoagulant effects. Recent data indicated that sCD40L plays a significant role in septic patients. The aim of the present study was to determine sCD40L changes in surgical patients without sepsis (SWS) and surgical sepsis patients (SS) during the first 3 days after intensive care unit (ICU) admission and to observe the association between sCD40L and mortality. Methods Time changes in sCD40L levels were assessed for 3 days after ICU admission in 49 patients with SS and compared with those in 19 SWS patients. Serum sCD40L concentration was detected by ELISA. Survival at 28 days served as the endpoint. Results SS had significantly higher sCD40L levels than SWS and control patients. We observed an association between sCD40L levels ≥1028.75 pg/mL at day 2 and 28-day mortality (odds ratio = 7.888; 95% confidence interval = 1.758 to 35.395; P = 0.007). We could not discover any significant differences in sex, presence of septic shock, site of infection, length of stay in the ICU, PaO2/FiO2 ratio, incidence of AKI, ARDS, or type of surgery between nonsurvivors and survivors. Conclusions Septic patients show persistently higher circulating sCD40L levels in the first 3 days after ICU admission, and serum sCD40L levels are associated with the mortality of patients with sepsis. Thus, serum sCD40L may be used as a reliable biomarker and therapeutic target in sepsis.

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Movement of Calcium Ions and their Role in the Activation of Platelets

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648654 ◽

1978 ◽

Vol 40 (02) ◽

pp. 212-218 ◽

Cited By ~ 60

Author(s):

P Massini ◽

R Käser-Glanzmann ◽

E F Lüscher

Keyword(s):

Plasma Membrane ◽

Platelet Activation ◽

Calcium Ions ◽

Binding Sites ◽

Shape Change ◽

Release Reaction ◽

Dense Bodies ◽

Activated Platelets ◽

Different Types ◽

Ca Ions

SummaryThe increase of the cytoplasmic Ca-concentration plays a central role in the initiation of platelet activation. Four kinds of movements of Ca-ions are presumed to occur during this process: a) Ca-ions liberated from membranes induce the rapid shape change, b) Vesicular organelles release Ca-ions into the cytoplasm which initiate the release reaction, c) The storage organelles called dense bodies, secrete their contents including Ca-ions to the outside during the release reaction, d) At the same time a rearrangement of the plasma membrane occurs, resulting in an increase in its permeability for Ca-ions as well as in an increase in the number of Ca-binding sites.Since most processes occurring during platelet activation are reversible, the platelet must be equipped with a mechanism which removes Ca-ions from the cytoplasm. A vesicular fraction obtained from homogenized platelets indeed accumulates Ca actively. This Ca- pump is stimulated by cyclic AMP and protein kinase; it may be involved in the recovery of platelets after activation.It becomes increasingly clear that the various manifestations of platelet activation are triggered by a rise in the cytoplasmic Ca2+-concentration. The evidence for this and possible mechanisms involved are discussed in some detail in the contributions by Detwiler et al. and by Gerrard and White to this symposium. In this article we shall discuss four different types of mobilization of Ca-ions which occur in the course of the activation of platelets. In addition, at least one transport step involved in the removal of Ca2+ must occur during relaxation of activated platelets.

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