scholarly journals Tualang Honey Supplementation Reduces Blood Oxidative Stress Levels/Activities in Postmenopausal Women

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Nazlahshaniza Shafin ◽  
Zahiruddin Othman ◽  
Rahimah Zakaria ◽  
Nik Hazlina Nik Hussain
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Postmenopausal Women ◽  
Reduced Glutathione ◽  
Oxidized Glutathione ◽  
Plasma Glutathione Peroxidase ◽  
Stress Levels ◽  
Plasma Glutathione ◽  
Progestin Therapy ◽  
Tualang Honey

This study aimed to investigate the ability of Tualang honey (TH), a phytoestrogen, to reduce blood oxidative stress levels/activities in postmenopausal women and compared the effects with the standard estrogen progestin therapy (EPT). A total of 78 healthy postmenopausal women were randomly assigned to one of two groups; EPT group received Femoston conti 1/5 (1 mg 17β-estradiol and 5 mg dydrogesterone), and TH group, received 20 g of TH supplement daily for 16 weeks. The reduced glutathione to oxidized glutathione ratio (GSH : GSSG), plasma glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), and 4-hydroxynonenal (4-HNE) levels/activities were measured at pre- and postinterventions using commercially available kits. The plasma GPx and CAT activities were notably increased, and plasma 4-HNE level was significantly decreased in postmenopausal women who received EPT and TH supplementation. TH supplementation for 16 weeks was able to reduce blood oxidative stress levels/activities of postmenopausal women comparable to those who received EPT. Thus, TH has a potential to be used as antioxidant therapy to combat oxidative stress-induced neurodegenerative diseases. This trial is registered with NCT01300676.

scholarly journals Spirulina maxima and its effect on antioxidant activity in fructose induced oxidative stress with histopathological observations

2015 ◽  
Vol 62 (2) ◽  
pp. 13-19
Author(s):  
Urmila Jarouliya ◽  
Anish Zacharia ◽  
Raj K. Keservani ◽  
Godavarthi B.K.S Prasad
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Blood Glucose ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Therapeutic Effects ◽  
Thiobarbituric Acid Reactive Substances ◽  
Spirulina Maxima

Abstract Diabetes mellitus is a metabolic disorder characterised by hyperglycemia and oxidative stress. The aim of the present study is to explore the antioxidant effect of Spirulina maxima in rat model along with the histopathological observations. Diabetes was induced by feeding 10% fructose solution orally to Wistar rats (n = 6) for 30 days, analysed for plasma blood glucose and the markers of the oxidative stress [catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS)]. These biochemical studies were associated with histopathological examination of liver and kidney sections. The microalga Spirulina maxima being rich in proteins and other essential nutrients is widely used as a food supplement. S. maxima at a dose of 5 and 10% per kg and the metformin (500 mg/kg) as reference drug were given orally for 30 days to the diabetic rats. Diabetic rats showed significant (p < 0.001) elevations in plasma blood glucose, thiobarbituric acid-reactive substances and significant reduction in catalase, superoxide dismutase and reduced glutathione activity. Oral administration of 5 and 10% aqueous extract of S. maxima for 30 days restored not only of blood glucose levels but also markers of oxidative stress. Histopathological observations of tissues manifested that the S. maxima administration had the protective and therapeutic effects against fructose-induced abnormalities in diabetic rats. It is concluded that S. maxima is effective in reinstating the antioxidant activity in addition to its antidiabetic effect in type 2 diabetic rats.

scholarly journals Relationship between serum DHEAS and oxidative stress levels of body mass index in healthy postmenopausal women

Redox Report ◽  
2016 ◽  
Vol 21 (2) ◽  
pp. 61-66 ◽  
Author(s):  
Burhan Goy ◽  
Murat Atmaca ◽  
Mehmet Aslan ◽  
Rıfkı Ucler ◽  
Murat Alay ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Mass Index ◽  
Postmenopausal Women ◽  
Body Mass ◽  
Stress Levels ◽  
And Oxidative Stress

scholarly journals Effect of a mitochondria-targeted vitamin E derivative on mitochondrial alteration and systemic oxidative stress in mice

2011 ◽  
Vol 106 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Gaowei Mao ◽  
George A. Kraus ◽  
Ikyon Kim ◽  
Michael E. Spurlock ◽  
Theodore B. Bailey ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Sod Activity ◽  
Mitochondrial Density ◽  
Plasma Glutathione Peroxidase ◽  
Systemic Oxidative Stress ◽  
Plasma Glutathione ◽  
Subsarcolemmal Mitochondria ◽  
Average Size ◽  
Dietary Treatments

The objective of the present study was to determine whether a mitochondria-targeted vitamin E derivative (MitoVit E) would affect certain mitochondrial parameters, as well as systemic oxidative stress. A total of sixty-four mice were fed a high-fat (HF) diet for 5 weeks. They were then switched to either a low-fat (LF) or a medium-fat (MF) diet, and administered orally with MitoVit E (40 mg MitoVit E/kg body weight) or drug vehicle (10 % (v/v) ethanol in 0·9 % (w/v) NaCl solution), every other day for 5 weeks. Mitochondrial ATP and H2O2 production rates in both the liver and the gastrocnemius were not affected by MitoVit E administration in either LF or MF diet-fed mice. However, the number and average size of the subsarcolemmal mitochondria, but not the intermyofibrillar mitochondria, from the soleus muscle were significantly higher in the MF group receiving MitoVit E (MF-E) than in the MF group receiving vehicle only (MF-C). After the mice were switched from the HF diet to the four dietary treatments (LF-C, LF-E, MF-C and MF-E), the decrease in urinary isoprostane concentration was significantly greater in the LF-E group than in the other three groups during the whole study (weeks 6–10). In addition, MitoVit E significantly increased plasma superoxide dismutase (SOD) activity in the MF diet-fed group without affecting plasma glutathione peroxidase activity or H2O2 levels. Overall, these data suggest that MitoVit E affects subsarcolemmal mitochondrial density and systemic oxidative stress parameters such as plasma SOD activity and urinary isoprostane concentration.

scholarly journals Characterization of Oxidative Stress in Various Tissues of Diabetic and Galactose-fed Rats

2001 ◽  
Vol 2 (3) ◽  
pp. 211-216 ◽  
Author(s):  
Robert M. Strother ◽  
Tonya G. Thomas ◽  
Mary Otsyula ◽  
Ruth A. Sanders ◽  
John B. Watkins III
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Rat Model ◽  
Catalase Activity ◽  
Diabetic Rats ◽  
Oxidized Glutathione ◽  
Hepatic Glutathione ◽  
Reduced And Oxidized Glutathione

Rats fed a galactose-rich diet have been used for several years as a model for diabetes to study, particularly in the eye, the effects of excess blood hexoses. This study sought to determine the utility of galactosemia as a model for oxidative stress in extraocular tissues by examining biomarkers of oxidative stress in galactose-fed rats and experimentally-induced diabetic rats. Sprague-Dawley rats were divided into four groups: experimental control; streptozotocin-induced diabetic; insulin-treated diabetic; and galactose-fed. The rats were maintained on these regimens for 30 days, at which point the activities of catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase, as well as levels of lipid peroxidation and reduced and oxidized glutathione were determined in heart, liver, and kidney. This study indicates that while there are some similarities between galactosemic and diabetic rats in these measured indices of oxidative stress (hepatic catalase activity levels and hepatic and renal levels of oxidized glutathione in both diabetic and galactosemic rats were significantly decreased when compared to normal), overall the galactosemic rat model is not closely parallel to the diabetic rat model in extra-ocular tissues. In addition, several effects of diabetes (increased hepatic glutathione peroxidase activity, increased superoxide dismutase activity in kidney and heart, decreased renal and increased cardiac catalase activity) were not mimicked in galactosemic rats, and glutathione concentration in both liver and heart was affected in opposite ways in diabetic rats and galactose- fed rats. Insulin treatment reversed/prevented the activity changes in renal and cardiac superoxide dismutase, renal and cardiac catalase, and hepatic glutathione peroxidase as well as the hepatic changes in lipid peroxidation and reduced and oxidized glutathione, and the increase in cardiac glutathione. Thus, prudence should be exercised in the use of experimentally galactosemic rats as a model for diabetes until the correspondence of the models has been more fully characterized.

scholarly journals Effect of Nandrolone Treatment with And Without Resistance Training on Superoxide Dismutase Concentration and Pathology of Kidney Tissue in Rats

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Esfandiyar Heidari ◽  
Seyed Ali Hosseini ◽  
Mohammad Ali Azarbayjani
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Resistance Training ◽  
Anabolic Steroids ◽  
Kidney Tissue ◽  
Sod Activity ◽  
Significant Difference ◽  
Stress Levels ◽  
Sh Group ◽  
Male Wistar Rats

Background: The prevalence of anabolic steroids abuse in athletes and non-athletes is associated with the risk of injury to various organs, but there are limited studies of oxidative changes in kidney tissue following nandrolone (N) administration. Objectives: the aim of this study was to investigate the effect of N treatment with and without resistance training (RT) on superoxide dismutase (SOD) concentration and tissue pathology of kidney tissue in rats. Methods: In this experimental study, 20 male Wistar rats were randomly divided into four groups of five rats including 1) control (C), 2) sham (normal saline) (Sh), 3) N, and 4) N + RT. Groups 3 and 4 received 10 mg/kg N peritoneally, and the N + RT group performed 1 m ladder climbing for eight weeks and three sessions per week. SOD levels of kidney tissue were measured by ELISA and radioimmunoassay. Hematoxylin-eosin (H&E) staining was used to evaluate oxidative stress levels in kidney tissue. One-way ANOVA with Bonferroni’s post- hoc tests were used for analysis of research findings in SPSS version 22 (P ≤ 0.05). Results: SOD levels in the C group were higher than the Sh (P = 0.001), N (P = 0.001), and N + RT (P = 0.001) groups. SOD levels were lower in the Sh group than in the N (P = 0.049) and N + RT (P = 0.001) groups. However, there was no significant difference in SOD levels in the N + RT group and N group (P = 0.28). Also, oxidative stress levels were normal in tissue studies in all groups. Conclusions: It seems that Ntreatment with and without RT reduces SOD activity in kidney tissue, but more studies are needed in this regard given the normality of tissue oxidative stress results.

Oxidative stress profile during postpartum period in Surti goats

2016 ◽  
Author(s):  
Tanvi D. Manat ◽  
Sandhya S. Chaudhary ◽  
Virendra Kumar Singh ◽  
Sanjay B. Patel ◽  
Kuldeep Kumar Tyagi
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Critical Care ◽  
Uric Acid ◽  
Glutathione Peroxidase ◽  
Postpartum Period ◽  
Reduced Glutathione ◽  
Stress Profile ◽  
Blood Samples

Present study was conducted to investigate postpartum oxidative stress in 20 Surti goats. Blood samples were collected on 0, 7th, 14th, 21st, 30th and 45th days postpartum and analysed for Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), lipid peroxidation (LPO), reduced Glutathione (GSH) and uric acid. SOD differed significantly between 0, 14th and 21st day postpartum. GPx was significantly low on 14th day and then increased significantly (P<0.01) up to 45th day. Significant (P<0.01) difference was observed between days except 0 and 21st. LPO increased significantly (P<0.01) from 0 to 14th day and then decreased non-significantly up to 45th day. Reduced glutathione was significantly (P<0.05) higher on 0 day. Uric acid was lowest on 0 day and highest on 45th day however they were non-significantly different on 7th, 14th, 30th and 45th day. It can be summarized that on 14th day post kidding, the values of SOD, GPx and GSH were lowest while LPO was highest. Uric acid was significantly (P<0.01) low on the day of kidding. Thus it may be concluded that in Surti goats the period from 0 day to 14th day postpartum is most stressful and critical care should be taken during this period. GPx, SOD along with LPO and GSH can be used as marker of stress during postpartum period.

scholarly journals Effect of silymarin on sodium fluoride-induced toxicity and oxidative stress in rat cardiac tissues

2012 ◽  
Vol 84 (4) ◽  
pp. 1121-1126 ◽  
Author(s):  
Seyed M. Nabavi ◽  
Seyed F. Nabavi ◽  
Akbar H. Moghaddam ◽  
William N. Setzer ◽  
Morteza Mirzaei
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Sodium Fluoride ◽  
Biochemical Parameters ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Glutathione Level ◽  
Thiobarbituric Acid Reactive Substances ◽  
Cardiac Tissues ◽  
And Oxidative Stress

This study aim to evaluate the protective effect of silymarin on sodium fluoride-induced oxidative stress in rat cardiac tissues. Animals were pretreated with silymarin at 20 and 10 mg/kg prior to sodium fluoride consumption (600 ppm through drinking water). Vitamin C at 10 mg/kg was used as standard antioxidant. There was a significant increase in thiobarbituric acid reactive substances level (59.36 ± 2.19 nmol MDA eq/g tissue) along with a decrease in antioxidant enzymes activity (64.27 ± 1.98 U/g tissue for superoxide dismutase activity and 29.17 ± 1.01 µmol/min/mg protein for catalase activity) and reduced glutathione level (3.8 ± 0.15 µg/mg protein) in the tissues homogenates of the sodium fluoride-intoxicated rats. Silymarin administration to animals before sodium fluoride consumption modified the levels of biochemical parameters.

Gender comparisons of exercise-induced oxidative stress: influence of antioxidant supplementation

2007 ◽  
Vol 32 (6) ◽  
pp. 1124-1131 ◽  
Author(s):  
Allan H. Goldfarb ◽  
Michael J. McKenzie ◽  
Richard J. Bloomer
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Reduced Glutathione ◽  
Protein Carbonyls ◽  
Plasma Vitamin ◽  
Oxidized Glutathione ◽  
Antioxidant Supplementation ◽  
Plasma Vitamin E ◽  
Exercise Induced ◽  
Response To Exercise

The purpose of this study was to determine the influence of gender and antioxidant supplementation on exercise-induced oxidative stress. Twenty-five men and 23 women ran for 30 min at 80% VO2 max, once before and once after 2 weeks of supplementation, and again after a 1-week wash-out period. Subjects were randomly assigned to either placebo (P), antioxidant (A: 400 IU vitamin E + 1 g vitamin C), or a fruit and vegetable powder (FV) treatment. Blood was obtained at rest and immediately after exercise. Before supplementation, women had higher resting reduced glutathione, total glutathione, and plasma vitamin E compared with men. With both A and FV supplementations, plasma vitamin E gender differences disappeared. Protein carbonyls, oxidized glutathione, and malondialdehyde all increased similarly for both genders in response to exercise. Both A and FV attenuated the reduced glutathione decrease and the oxidized glutathione and protein carbonyls increase compared with P, with no gender differences. 8-Hydroxydeoxyguanosine was lower with treatment A compared with FV and P only for men. Plasma vitamin C increased 39% (A) and 21% (FV) compared with P. These data indicate that women have higher resting antioxidant levels than men. Markers of oxidative stress increased similarly in both genders in response to exercise of similar intensity and duration. Two weeks of antioxidant supplementation can attenuate exercise-induced oxidative stress equally in both genders.

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