scholarly journals Role of Dried Fruits ofCarissa carandasas Anti-Inflammatory Agents and the Analysis of Phytochemical Constituents by GC-MS

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
N. Anupama ◽  
G. Madhumitha ◽  
K. S. Rajesh
Keyword(s):  
Methanol Extract ◽  
Inflammatory Diseases ◽  
Control Group ◽  
Significant Activity ◽  
Dependent Manner ◽  
Paw Edema ◽  
Dried Fruits ◽  
Carissa Carandas ◽  
Dried Fruit ◽  
Anti Inflammatory

Inflammation plays an important role in various diseases with high prevalence within populations such as rheumatoid arthritis, ulcer, atherosclerosis, and asthma. Many drugs are available in the market for inflammatory diseases. They exhibit several unwanted side effects to humans. Therefore, alternative treatments with safer compounds are needed.Carissa carandasplant is used in traditional medicinal system for its various diseases curing property. In the present study, we examined the anti-inflammatory effects of dried fruit methanol extract on carrageenan-induced hind paw edema in rats.C. carandaswas defatted with petroleum ether, followed by methanol extraction. The methanol extracts of the dried fruits ofCarissa carandaswere given orally to the experimental rats caused significant activity (P≤0.05) when compared with the control group. The maximum inhibition of paw edema was found to be in Group V, that is, 76.12% with inhibition of paw volume in a dose-dependent manner. The anti-inflammatory activity of the methanol extract of the dried fruits shows that the presence of potential constituents present in this extract may provide assistance in the drug discovery process. The phytochemical compounds of the extract were screened by GC-MS analysis and it was found that 11 compounds are present in methanol extract of dried fruits ofCarissa carandas.

Evaluation of Antinociceptive, Anti-inflammatory and Antipyretic Effects of Strobilanthes cusia Leaf Extract in Male Mice and Rats

2003 ◽  
Vol 31 (01) ◽  
pp. 61-69 ◽  
Author(s):  
Yu-Ling Ho ◽  
Kuo-Ching Kao ◽  
Huei-Yann Tsai ◽  
Fu-Yu Chueh ◽  
Yuan-Shiun Chang
Keyword(s):  
Leaf Extract ◽  
Formalin Test ◽  
Methanol Extract ◽  
Inflammatory Diseases ◽  
Dependent Manner ◽  
Male Mice ◽  
Influenza Epidemic ◽  
Paw Edema ◽  
Anti Inflammatory ◽  
Dose Dependent

The leaf of Strobilanthes cusia (Acanthaceae), popularly known as Da-Ching-Yeh, has been commonly used in traditional Chinese medicine. It is used for influenza, epidemic cerebrospinal meningitis, encephalitis B, viral pneumonia and mumps. It is also used to treat sore throat, aphthae and inflammatory diseases with redness of skin, etc. In this study, we evaluated the antinociceptive, anti-inflammatory and antipyretic effects of methanol extract of Strobilanthes cusia leaf. The results showed that the extract significantly inhibited the writhing responses of mice and decreased the licking time on both the early and late phases of the formalin test in a dose-dependent manner. It also reduced the paw edema induced by carrageenan in rats. In addition, it potently attenuated pyrexia induced by lipopolysaccharide.

scholarly journals Anti-Inflammatory Activity of Mimosa hamata (Willd.)

2019 ◽  
Vol 9 (5-s) ◽  
pp. 34-39
Author(s):  
N. I. Khan ◽  
B.C. Hatapakki
Keyword(s):  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Control Group ◽  
Albino Rats ◽  
Significant Activity ◽  
Dependent Manner ◽  
Paw Edema ◽  
Cotton Pellet ◽  
Cotton Pellet Granuloma ◽  
Anti Inflammatory

Anti-inflammatory activity of ethanolic extract of roots, stems and leaves Mimosa hamata was investigated at the doses of 200 and 400 mg/kg using carrageenan induced paw edema and cotton pellet granuloma technique in albino rats. The stem extracts showed significant activity in dose dependent manner as compared to control group. The observations suggested that the extract of M. hamata were effective in exudative and proliferative phases of inflammation i.e. in acute and chronic inflammation. The results obtained indicate that M. hamata has an anti-inflammatory activity that supports the folk medicinal use of the plant. Keywords: Mimosa hamata, anti-inflammatory activity, carrageenan induced paw edema, cotton pellet granuloma.

scholarly journals Analgesic and Anti-Inflammatory Activities of Methanol Extract ofFicus pumilaL. in Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Chi-Ren Liao ◽  
Chun-Pin Kao ◽  
Wen-Huang Peng ◽  
Yuan-Shiun Chang ◽  
Shang-Chih Lai ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Inflammatory Diseases ◽  
Paw Edema ◽  
Tissue Destruction ◽  
Hplc Fingerprint ◽  
Analgesic Effects ◽  
Inflammatory Mechanism ◽  
Tnf Α ◽  
Anti Inflammatory

This study investigated possible analgesic and anti-inflammatory mechanisms of the methanol extract ofFicus pumila(FPMeOH). Analgesic effects were evaluated in two models including acetic acid-induced writhing response and formalin-induced paw licking. The results showedFPMeOHdecreased writhing response in the acetic acid assay and licking time in the formalin test. The anti-inflammatory effect was evaluated by λ-carrageenan-induced mouse paw edema and histopathological analyses.FPMeOHsignificantly decreased the volume of paw edema induced by λ-carrageenan. Histopathologically,FPMeOHabated the level of tissue destruction and swelling of the edema paws. This study indicated anti-inflammatory mechanism ofFPMeOHmay be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx, and GRd in the liver. Additionally,FPMeOHalso decreased the level of inflammatory mediators such as IL-1β, TNF-α, and COX-2. HPLC fingerprint was established and the contents of three active ingredients, rutin, luteolin, and apigenin, were quantitatively determined. This study provided evidence for the classical treatment ofFicus pumilain inflammatory diseases.

scholarly journals IN VITRO ANTIOXIDANT AND IN VIVO ANTI-INFLAMMATORY ACTIVITY OF THE AERIAL PART OF BLUMEA ERIANTHA DC

2018 ◽  
Vol 10 (7) ◽  
pp. 75 ◽  
Author(s):  
Urmila U. Tambewagh ◽  
Supada Rambhau Rojatkar
Keyword(s):  
Antioxidant Activity ◽  
Aerial Part ◽  
Methanol Extract ◽  
Reducing Power ◽  
Dependent Manner ◽  
Paw Edema ◽  
Anti Inflammatory ◽  
In Vitro Antioxidant Activity

Objective: Objective of the present study was to carry out in vivo anti-inflammatory and in vitro antioxidant activity of methanol extract of aerial part of the Blumea eriantha DC belonging to family Asteraceae.Methods: The shade dried aerial part of B. eriantha (0.5 kg) was powdered and extracted with methanol (1.5 x 3L) at room temperature (24h x 3). After filtration combined all the three extracts and were concentrated on rotary evaporator under reduced pressure at 40 °C, thereby providing crude methanol extract which was subsequently employed for further studies. Anti-inflammatory effect was studied by carrageenan-induced paw edema model in rats at dose level 100, 200, and 400 mg/kg. Acute oral toxicity study and in vitro antioxidant potential of the extract was also studied. The in vitro antioxidant activity of methanol extract of aerial part of Blumea eriantha DC was evaluated against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) and hydroxyl (OH) radicalscavenging and reducing power assays.Results: The results indicate that methanol extract of Blumea eriantha (BEME, 400 mg/kg) exhibited significant inhibition (p<0.001) of increase in paw edema at 5th h. IC50 value of BEME showed significant antioxidant activity. The extract exhibits promising free radical scavenging effect of DPPH, H2O2, OH and reducing power in a dose-dependent manner up to 100µg/ml concentration while the reference standard Ascorbic acid demonstrated more scavenging potential than the methanol extract of Blumea eriantha The methanol extract was found to be safe at the dose of 2000 mg/kg.Conclusion: The results of the experimental study confirmed that methanol extract of Blumea eriantha DC possesses significant anti-inflammatory and antioxidant activity.

scholarly journals Comparative Evaluation of Analgesic, Antipyretic & Anti-Inflammatory Effects of Various Extracts of Dried Fruit of Illicium verum Hook.f (Star anise) in Rodents

2021 ◽  
Vol 18 (9) ◽  
Author(s):  
Hafiza TUSEEF ◽  
Muhammad Liaquat RAZA ◽  
Tahira ASSAD
Keyword(s):  
Ethanol Extract ◽  
Maximum Effect ◽  
Control Group ◽  
Dependent Manner ◽  
Dried Fruit ◽  
Illicium Verum ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Dose Dependent ◽  
Different Doses

The current investigation was designed to evaluate the analgesic, antipyretic, and anti-inflammatory effects of various extracts (methanol, ethanol, and aqueous) of dried fruit of Illicium verum hook.f, using 3 different doses (150, 250, and 350 mg/kg p.o) to verify the traditional uses of this spice. In the hot plate model of analgesia, ethanol extract showed a significant reduction in pain in a dose-dependent manner compared to the control group. The maximum effect was observed at 350 mg/kg dosage i.e., 16.90±0.17 s compared to the control group i.e., 5.03±0.05 s. The antipyretic activity was assessed in rats by Brewer’s yeast induction.  The methanol and ethanol extracts produced a significant reduction in rectal temperature compared to the control group throughout the three doses. The maximum effect was observed at 350 mg/kg dosage of ethanol extract i.e., 37.1±0.8* compared to the control i.e., 39.1±0.3. In the paw edema model, methanol and ethanol extracts disclosed a significant reduction in paw edema at 350 mg/kg of dose. The maximum effect was observed at 350 mg/kg dosage of ethanol extract i.e., 0.25±0.23* compared to the control i.e., 0.97±0.4. In a behavioral study, locomotor activity (rearing) and exploratory activity (grooming) in mice was reduced significantly at higher doses (350 mg/kg p.o) involving the three extracts. However, scratching was increased non-significantly at all doses compared to the control group. This study concluded that various extracts of Illicium verum hook.f showed significant analgesic, antipyretic, and anti-inflammatory effects at different doses in a dose-dependent manner with varying potencies. The ethanol extract was found to be more potent among all, followed by methanol and aqueous extracts, whereas maximum effects were observed at 350 mg/kg of dose.

scholarly journals Analgesic and Anti-Inflammatory Activities of the Methanol Extract fromPogostemon cablin

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Tsung-Chun Lu ◽  
Jung-Chun Liao ◽  
Tai-Hung Huang ◽  
Ying-Chih Lin ◽  
Chia-Yu Liu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Second Phase ◽  
Dependent Manner ◽  
Paw Edema ◽  
Pogostemon Cablin ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Factor Α

Pogostemon cablin(PC) is a herbal medicine traditionally applied to treat not only common cold, nausea and diarrhea but also headache and fever. The aim of this study was to investigate the analgesic and anti-inflammatory properties of standardized PC methanol extract (PCMeOH)in vivo. Investigations were performed in mice with two analgesic models. One was acetic acid-induced writhing response and the other formalin-induced paw licking. The anti-inflammatory effect was tested by λ-carrageenan (Carr)-induced mice paw edema. These analgesic experimental results indicated that PCMeOH (1.0 g/kg) decreased the acetic acid-induced writhing responses and PCMeOH (0.5 and 1.0 g/kg) decreased the licking time in the second phase of the formalin test. Moreover, Carr-induced paw edema inflammation was significantly reduced in a dose-dependent manner when PCMeOH (0.5 and 1.0 g/kg) was administered 3 and 4 h after the Carr injection. Mechanistic studies showed that PCMeOH decreased the levels of malondialdehyde in the edema paw by increasing the activities of anti-oxidant enzymes, such as superoxide dismutase, glutathione peroxidase and glutathione reductase, in the liver and decreasing the cyclooxygenase 2 and tumor necrosis factor-α activities in the edema paw. This study has demonstrated the analgesic and anti-inflammatory effects of PCMeOH, thus verifying its popular use in traditional medicine.

scholarly journals Evaluation of Anti-Inflammatory Activities of a Triterpene β-Elemonic Acid in Frankincense In Vivo and In Vitro

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1187 ◽  
Author(s):  
Yue Zhang ◽  
Ying-li Yu ◽  
Hua Tian ◽  
Ru-yu Bai ◽  
Ya-nan Bi ◽  
...  
Keyword(s):  
Inflammatory Diseases ◽  
Interleukin 10 ◽  
The Body ◽  
Raw264.7 Cells ◽  
Control Group ◽  
Intragastric Administration ◽  
Dependent Manner ◽  
Anti Inflammatory

The purpose of this research was to extract and separate the compounds from frankincense, and then evaluate their anti-inflammatory effects. The isolated compound was a representative tetracyclic triterpenes of glycine structure according to 1H-NMR and 13C-NMR spectra, which is β-elemonic acid (β-EA). We determined the content of six different localities of frankincense; the average content of β-EA was 41.96 mg/g. The toxic effects of β-EA administration (400, 200, 100 mg/kg) for four weeks in Kunming (KM) mice were observed. Compared with the control group, the body weight of mice, the visceral coefficients and serum indicators in the β-EA groups showed no systematic variations. The anti-inflammatory effects of β-EA were evaluated in LPS-induced RAW264.7 cells, xylene-induced induced ear inflammation in mice, carrageenin-induced paw edema in mice, and cotton pellet induced granuloma formation in rats. β-EA inhibited overproduction of tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), monocyte chemotactic protein 1 (MCP-1), soluble TNF receptor 1 (sTNF R1), Eotaxin-2, Interleukin 10 (IL-10) and granulocyte colony-stimulating factor (GCSF) in the RAW264.7 cells. Intragastric administration with β-EA (300, 200, and 100 mg/kg in mice, and 210, 140, and 70 mg/kg in rats) all produced distinct anti-inflammatory effects in vivo in a dose-dependent manner. Following treatment with β-EA (300 mg/kg, i.g.), the NO level in mice ears and PGE2 in mice paws both decreased (p < 0.01). In conclusion, our study indicates that β-EA could be a potential anti-inflammatory agent for the treatment of inflammatory diseases.

scholarly journals Anti-Inflammatory Property of the Ethanol Extract of the Root and Rhizome ofPogostemon cablin(Blanco) Benth

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Chu-Wen Li ◽  
Xiao-Li Wu ◽  
Xiao-Ning Zhao ◽  
Zu-Qing Su ◽  
Hai-Ming Chen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Inflammatory Diseases ◽  
Ethanol Extract ◽  
Dependent Manner ◽  
Paw Edema ◽  
Ear Edema ◽  
Pogostemon Cablin ◽  
Mouse Ear ◽  
Anti Oxidant ◽  
Anti Inflammatory

The aim of this study was to investigate the anti-inflammatory property of the ethanol extract of the root and rhizome ofPogostemon cablin(ERP). The anti-inflammatory effect was evaluated using four animal models including xylene-induced mouse ear edema, acetic acid-induced mouse vascular permeability, carrageenan-induced mouse pleurisy, and carrageenan-induced mouse hind paw edema. Results indicated that oral administration of ERP (120, 240, and 480 mg/kg) significantly attenuated xylene-induced ear edema, decreased acetic acid-induced capillary permeability, inhibited carrageenan-induced neutrophils recruitment, and reduced carrageenan-induced paw edema, in a dose-dependent manner. Histopathologically, ERP (480 mg/kg) abated inflammatory response of the edema paw. Preliminary mechanism studies demonstrated that ERP decreased the level of MPO and MDA, increased the activities of anti-oxidant enzymes (SOD, GPx, and GRd), attenuated the productions of TNF-α, IL-1β, IL-6, PGE2and NO, and suppressed the activities of COX-2 and iNOS. This work demonstrates that ERP has considerable anti-inflammatory potential, which provided experimental evidences for the traditional application of the root and rhizome ofPogostemon cablinin inflammatory diseases.

scholarly journals Evaluation on Anti-Inflammatory, Analgesic, Antitumor, and Antioxidant Potential of Total Saponins fromNigella glanduliferaSeeds

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Jun Zhao ◽  
Fang Xu ◽  
Hua Huang ◽  
Zhengyi Gu ◽  
Linlin Wang ◽  
...  
Keyword(s):  
Inflammatory Diseases ◽  
Radical Scavenging ◽  
Control Group ◽  
Dependent Manner ◽  
Scavenging Effect ◽  
Ageing Model ◽  
Radical Scavenging Effect ◽  
Antioxidant Agent ◽  
Anti Inflammatory ◽  
Dose Dependent

Nigella glanduliferaseeds are used as a spice or remedy for the treatment of various inflammatory diseases. This study aimed to investigate analgesic (writhing test), anti-inflammatory (ear-induced edema, vascular permeability test), antioxidant, and antitumor activities of total saponins from this plant (TSN). TSN (6, 12, and 24 mg/kg) were exhibited analgesic and anti-inflammatory activities in a dose-dependent manner (P<0.05). In D-galactose-induced ageing model, TSN significantly increased the plasma superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities (P<0.05) and decreased the malondialdehyde (MDA) level compared to control group (P<0.05). DPPH radical scavenging effect of TSN was also found. Moreover, TSN (20 mg/mL) showed 86.75% and 88.26% inhibition of the growth on Bel-7402 and Hela cells, respectively. Five compounds were further isolated and identified from TSN as Nigella A, B, C, D, and nigeglanoside, of which the content of Nigella A was60.36±1.25 g/100 g TSN by HPLC-ELSD method. Altogether, these results suggest that TSN could be considered as a potential analgesic, anti-inflammatory, antitumor, and antioxidant agent.

scholarly journals Analgesic and Anti-Inflammatory Activities of Methanol Extract ofCissus repensin Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Ching-Wen Chang ◽  
Wen-Te Chang ◽  
Jung-Chun Liao ◽  
Yung-Jia Chiu ◽  
Ming-Tsuen Hsieh ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Formalin Test ◽  
Methanol Extract ◽  
Inflammatory Diseases ◽  
Paw Edema ◽  
Tissue Destruction ◽  
Inflammatory Mechanism ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

The aim of this study was to investigate possible analgesic and anti-inflammatory mechanisms of theCRMeOH. Analgesic effect was evaluated in two models including acetic acid-induced writhing response and formalin-induced paw licking. The anti-inflammatory effect was evaluated byλ-carrageenan-induced mouse paw edema and histopathologic analyses. The results showed thatCRMeOH(500 mg/kg) decreased writhing response in the acetic acid assay and licking time in the formalin test.CRMeOH(100 and 500 mg/kg) significantly decreased edema paw volume at 4th to 5th hours afterλ-carrageenan had been injected. Histopathologically,CRMeOHabated the level of tissue destruction and swelling of the edema paws. These results were indicated that anti-inflammatory mechanism ofCRMeOHmay be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx, and GRd in the liver. Additionally,CRMeOHalso decreased IL-1β, IL-6, NFκB, TNF-α, COX-2, and iNOS levels. The contents of two active ingredients, ursolic acid and lupeol, were quantitatively determined. This paper demonstrated possible mechanisms for the analgesic and anti-inflammatory effects ofCRMeOHand provided evidence for the classical treatment ofCissus repensin inflammatory diseases.

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