scholarly journals Evaluation on Anti-Inflammatory, Analgesic, Antitumor, and Antioxidant Potential of Total Saponins fromNigella glanduliferaSeeds

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Jun Zhao ◽  
Fang Xu ◽  
Hua Huang ◽  
Zhengyi Gu ◽  
Linlin Wang ◽  
...  
Keyword(s):  
Inflammatory Diseases ◽  
Radical Scavenging ◽  
Control Group ◽  
Dependent Manner ◽  
Scavenging Effect ◽  
Ageing Model ◽  
Radical Scavenging Effect ◽  
Antioxidant Agent ◽  
Anti Inflammatory ◽  
Dose Dependent

Nigella glanduliferaseeds are used as a spice or remedy for the treatment of various inflammatory diseases. This study aimed to investigate analgesic (writhing test), anti-inflammatory (ear-induced edema, vascular permeability test), antioxidant, and antitumor activities of total saponins from this plant (TSN). TSN (6, 12, and 24 mg/kg) were exhibited analgesic and anti-inflammatory activities in a dose-dependent manner (P<0.05). In D-galactose-induced ageing model, TSN significantly increased the plasma superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities (P<0.05) and decreased the malondialdehyde (MDA) level compared to control group (P<0.05). DPPH radical scavenging effect of TSN was also found. Moreover, TSN (20 mg/mL) showed 86.75% and 88.26% inhibition of the growth on Bel-7402 and Hela cells, respectively. Five compounds were further isolated and identified from TSN as Nigella A, B, C, D, and nigeglanoside, of which the content of Nigella A was60.36±1.25 g/100 g TSN by HPLC-ELSD method. Altogether, these results suggest that TSN could be considered as a potential analgesic, anti-inflammatory, antitumor, and antioxidant agent.

scholarly journals Comparison of Biological Activities of Different Parts of Asparagus

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 771-771
Author(s):  
Jiseon Park ◽  
Jaegeel Lim
Keyword(s):  
Inhibitory Activity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Control Group ◽  
Total Phenol Content ◽  
No Production ◽  
Dpph Radical ◽  
Scavenging Effect ◽  
Radical Scavenging Effect ◽  
Different Parts

Abstract Objectives Asparagus is a spring vegetable, a flowering perennial plant species belonging to the lily family. Technavio's analysts forecast the Global Asparagus Market to grow at a CAGR of 3.15% during the period 2018–2022. All parts of asparagus, including roots, are rich in bioactive chemicals. However, other parts except asparagus spears are discarded. This study compared the physiological activity of different parts of asparagus and tried to improve the utilization of other parts. Methods Asparagus was divided into root, spear (5 cm from the bottom, 25 cm from the top, respectively), leaf and stem in order and used after lyophilized. Total phenol content, antioxidant activity (DPPH radical elimination ability, ABTS radical elimination ability), α-amylase inhibitory activity, and the effects of nitric oxide (NO) production were measured. Results The total polyphenol content of asparagus was highest in the leaves (22.87 mg TAE/g) and lowest in 5 cm under part of the spear(3.03 mg TAE/g). DPPH radical scavenging effect (44.52% in 1 mg/mL, 92.84% in 5 mg/mL), and ABTS radical scavenging effect(15.58% in 1 mg/mL, 48.02% in 5 mg/1 mL) were the highest on the leaf. On 5 cm under part of the spear, α-amylase inhibitory activity (79.16%) was the higher than any other parts. NO generation was significantly hindered by LPS treatment group 5 cm under part of the spear (12.93 μM), 25 cm above the part of the spear (12.10 μM), root (11.68 μM), leaf(10.43 μM), and stem(9.70 μM) in order(P &lt; 0.001). All the extracts were treated with the cells at concentrations of 100, 500 and 1000 µg/mL for one day. As a result, the survival rate of the macrophages was not significantly different from that of the control group. So it was confirmed that there is no cytotoxicity. Conclusions Thus, these results suggest that the another parts of asparagus may be highly valuable as a high-quality functional component as well as its-antioxidant, antiinflammatory activities. we believe that the results obtained would be helpful in the development of nutraceutics. Funding Sources This study was supported by 2019 Regional Specialized Technology Development Project, Rural Development Administration, Republic of Korea.

scholarly journals Anti-Inflammatory, Antioxidant and Anti-Diarrheal Effects of Ethanol Extract of Stephania Japonica

1970 ◽  
Vol 46 (4) ◽  
pp. 437-442
Author(s):  
NU Ahmed ◽  
R Akter ◽  
Mohammed A Satter ◽  
MS Khan ◽  
F Islam ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dosage Level ◽  
Scavenging Effect ◽  
Ic50 Values ◽  
Radical Scavenging Effect ◽  
Anti Inflammatory ◽  
Free Radical Scavenging Effect ◽  
Inflammatory Effect

The present study was carried out to investigate anti-inflammatory, antioxidant and anti-diarrheal effect of ethanol extract of Stephania japonica. This study showed that the plant extract has significant (p<0.05) anti-inflammatory effect at all phases of carrageenan induced inflammation at a dose level 2g/kg. The DPPH free radical scavenging effect of the extract was compared with standard antioxidant ascorbic acid. IC50 values were found 33.57 μg/ml for the extract and 15.57 μg/ml for ascorbic acid. S. japonica extract at dosage level 2g/kg and 1g/kg decreased the gastrointestinal motility 36.56 and 21.53 %, respectively, in rats. The ethanol extract of the plant also reduced the total number of feces as well as wet feces of rats in castor oil-induced diarrheal model. The results revealed that the extract possesses promising anti-inflammatory, antioxidant and antidiarrheal activity. Keywords: Anti-inflammatory; Antioxidant; Anti-diarrheal; Stephania japonica DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9587 BJSIR 2011; 46(4): 437-442

scholarly journals Comparative Evaluation of Analgesic, Antipyretic & Anti-Inflammatory Effects of Various Extracts of Dried Fruit of Illicium verum Hook.f (Star anise) in Rodents

2021 ◽  
Vol 18 (9) ◽  
Author(s):  
Hafiza TUSEEF ◽  
Muhammad Liaquat RAZA ◽  
Tahira ASSAD
Keyword(s):  
Ethanol Extract ◽  
Maximum Effect ◽  
Control Group ◽  
Dependent Manner ◽  
Dried Fruit ◽  
Illicium Verum ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Dose Dependent ◽  
Different Doses

The current investigation was designed to evaluate the analgesic, antipyretic, and anti-inflammatory effects of various extracts (methanol, ethanol, and aqueous) of dried fruit of Illicium verum hook.f, using 3 different doses (150, 250, and 350 mg/kg p.o) to verify the traditional uses of this spice. In the hot plate model of analgesia, ethanol extract showed a significant reduction in pain in a dose-dependent manner compared to the control group. The maximum effect was observed at 350 mg/kg dosage i.e., 16.90±0.17 s compared to the control group i.e., 5.03±0.05 s. The antipyretic activity was assessed in rats by Brewer’s yeast induction.  The methanol and ethanol extracts produced a significant reduction in rectal temperature compared to the control group throughout the three doses. The maximum effect was observed at 350 mg/kg dosage of ethanol extract i.e., 37.1±0.8* compared to the control i.e., 39.1±0.3. In the paw edema model, methanol and ethanol extracts disclosed a significant reduction in paw edema at 350 mg/kg of dose. The maximum effect was observed at 350 mg/kg dosage of ethanol extract i.e., 0.25±0.23* compared to the control i.e., 0.97±0.4. In a behavioral study, locomotor activity (rearing) and exploratory activity (grooming) in mice was reduced significantly at higher doses (350 mg/kg p.o) involving the three extracts. However, scratching was increased non-significantly at all doses compared to the control group. This study concluded that various extracts of Illicium verum hook.f showed significant analgesic, antipyretic, and anti-inflammatory effects at different doses in a dose-dependent manner with varying potencies. The ethanol extract was found to be more potent among all, followed by methanol and aqueous extracts, whereas maximum effects were observed at 350 mg/kg of dose.

scholarly journals Inhibition of LPS-Induced Oxidative Damages and Potential Anti-Inflammatory Effects of Phyllanthus emblica Extract via Down-Regulating NF-κB, COX-2, and iNOS in RAW 264.7 Cells

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 270 ◽  
Author(s):  
Hui Min-David Wang ◽  
Ling Fu ◽  
Chia Chi Cheng ◽  
Rong Gao ◽  
Meng Yi Lin ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Reducing Power ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Dependent Manner ◽  
Phyllanthus Emblica ◽  
Fruit Extract ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Dose Dependent

Phyllanthus emblica is an edible nutraceutical and functional food in the Asia area with medicinal and nutritive importance. The fruit extract of P. emblica is currently considered to be one of the effective functional foods for flesh maintenance and disease treatments because of its antioxidative and immunomodulatory properties. We examined the antioxidant abilities of the fruit extract powder by carrying out 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, iron reducing power, and metal chelating activity analysis and showed excellent antioxidative results. In 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the result showed that the samples had no cytotoxic effect on RAW 264.7 cells even at a high concentration of 2 mg/mL. To investigate its immunomodulatory function, our estimation was to treat it with lipopolysaccharide (LPS) in RAW 264.7 cells to present anti-inflammatory capacities. The extract decreased reactive oxygen species (ROS) production levels in a dose-dependent manner measured by flow cytometry. We also examined various inflammatory mRNAs and proteins, including nuclear factor-κB (NF-κB), inducible nitric oxide synthases (iNOS), and cyclooxygenase-2 (COX-2). In quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blotting assay, all three targets were decreased by the extract, also in a dose-dependent manner. In conclusion, P. emblica fruit extract powder not only lessened antioxidative stress damages, but also inhibited inflammatory reactions.

Evaluation of Antinociceptive, Anti-inflammatory and Antipyretic Effects of Strobilanthes cusia Leaf Extract in Male Mice and Rats

2003 ◽  
Vol 31 (01) ◽  
pp. 61-69 ◽  
Author(s):  
Yu-Ling Ho ◽  
Kuo-Ching Kao ◽  
Huei-Yann Tsai ◽  
Fu-Yu Chueh ◽  
Yuan-Shiun Chang
Keyword(s):  
Leaf Extract ◽  
Formalin Test ◽  
Methanol Extract ◽  
Inflammatory Diseases ◽  
Dependent Manner ◽  
Male Mice ◽  
Influenza Epidemic ◽  
Paw Edema ◽  
Anti Inflammatory ◽  
Dose Dependent

The leaf of Strobilanthes cusia (Acanthaceae), popularly known as Da-Ching-Yeh, has been commonly used in traditional Chinese medicine. It is used for influenza, epidemic cerebrospinal meningitis, encephalitis B, viral pneumonia and mumps. It is also used to treat sore throat, aphthae and inflammatory diseases with redness of skin, etc. In this study, we evaluated the antinociceptive, anti-inflammatory and antipyretic effects of methanol extract of Strobilanthes cusia leaf. The results showed that the extract significantly inhibited the writhing responses of mice and decreased the licking time on both the early and late phases of the formalin test in a dose-dependent manner. It also reduced the paw edema induced by carrageenan in rats. In addition, it potently attenuated pyrexia induced by lipopolysaccharide.

scholarly journals An investigation of bioactive and free radical scavenging potential of moringa Oliefera leaf extracts

2019 ◽  
Vol 10 (3) ◽  
pp. 1575-1579
Author(s):  
Uzma Sayyed ◽  
Pratibha Pandey ◽  
Rohit K. Tiwari ◽  
Rafia Shekh ◽  
Preeti Bajpai
Keyword(s):  
Methanolic Extract ◽  
Inflammatory Diseases ◽  
Radical Scavenging ◽  
Morphological Characteristics ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Ic50 Value ◽  
Percentage Yield ◽  
Antioxidant Potency ◽  
Dose Dependent

Moringa oleifera Lam, commonly known as Sehjan belongs to the Moringaceae family. It is widely used for the treatment of infectious and inflammatory diseases. This study was an attempt to evaluate the morphological characteristics, percent yield, the bioactive and antioxidant potential of M. oleifera leaves that would help in elucidating a promising therapeutic and curative agent for the treatment of different ailments. The maximum percentage yield was obtained in methanolic extract (29.55%) of M. oliefera leaves. Qualitative analysis also revealed the maximum presence of all the metabolites in methanolic extract. Quantitative analysis revealed an appreciable presence of phenol (53.1 mg/g) flavonoids (47.7mg/g) and carotenoids (16.46 mg/g) in M. oleifera leaves. The methanolic extract had shown the maximum antioxidant potency in a dose-dependent manner during the evaluation of enzymatic (SOD and CAT) and nonenzymatic (DPPH and FRAP) antioxidants with minimum IC50 value. Thus, it could be concluded from the present study that methanolic leaf extract of M. oliefera could be amongst the principle extract for the antioxidant activity of M. oliefera, which could be used for the treatment of several ailments.

scholarly journals Evaluation of Anti-Inflammatory Activities of a Triterpene β-Elemonic Acid in Frankincense In Vivo and In Vitro

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1187 ◽  
Author(s):  
Yue Zhang ◽  
Ying-li Yu ◽  
Hua Tian ◽  
Ru-yu Bai ◽  
Ya-nan Bi ◽  
...  
Keyword(s):  
Inflammatory Diseases ◽  
Interleukin 10 ◽  
The Body ◽  
Raw264.7 Cells ◽  
Control Group ◽  
Intragastric Administration ◽  
Dependent Manner ◽  
Anti Inflammatory

The purpose of this research was to extract and separate the compounds from frankincense, and then evaluate their anti-inflammatory effects. The isolated compound was a representative tetracyclic triterpenes of glycine structure according to 1H-NMR and 13C-NMR spectra, which is β-elemonic acid (β-EA). We determined the content of six different localities of frankincense; the average content of β-EA was 41.96 mg/g. The toxic effects of β-EA administration (400, 200, 100 mg/kg) for four weeks in Kunming (KM) mice were observed. Compared with the control group, the body weight of mice, the visceral coefficients and serum indicators in the β-EA groups showed no systematic variations. The anti-inflammatory effects of β-EA were evaluated in LPS-induced RAW264.7 cells, xylene-induced induced ear inflammation in mice, carrageenin-induced paw edema in mice, and cotton pellet induced granuloma formation in rats. β-EA inhibited overproduction of tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), monocyte chemotactic protein 1 (MCP-1), soluble TNF receptor 1 (sTNF R1), Eotaxin-2, Interleukin 10 (IL-10) and granulocyte colony-stimulating factor (GCSF) in the RAW264.7 cells. Intragastric administration with β-EA (300, 200, and 100 mg/kg in mice, and 210, 140, and 70 mg/kg in rats) all produced distinct anti-inflammatory effects in vivo in a dose-dependent manner. Following treatment with β-EA (300 mg/kg, i.g.), the NO level in mice ears and PGE2 in mice paws both decreased (p < 0.01). In conclusion, our study indicates that β-EA could be a potential anti-inflammatory agent for the treatment of inflammatory diseases.

scholarly journals Antioxidant property, anti-inflammatory and analgesic effects of aqueous extracts of two onion bulbs varieties (Allium cepa L.)

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mang Yannick Dimitry ◽  
Panyo’o Akdowa Emmanuel ◽  
Djiogue Manejo Josiane Edith ◽  
Abdou Bouba Armand ◽  
Njintang Yanou Nicolas
Keyword(s):  
Aqueous Extract ◽  
Analgesic Activity ◽  
Analgesic Effect ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Control Group ◽  
Dependent Manner ◽  
Aqueous Extracts ◽  
Anti Inflammatory

Abstract Background oxidative stress and inflammation remain the main health problems often linked to degenerative diseases. The aim of this study was to evaluate antioxidant, analgesic and anti-inflammatory properties of onion growth in Cameroon. Methods Aqueous extracts were produced from two varieties of onion (Violet of galmi; Goudami) and were quantified for their polyphenols content. Concentrations of extracts (0.1, 0.5, 1, 1.5 mg/mL) were also prepared, their in vitro antioxidant properties evaluated by using standard methods. Anti-inflammatory effect of onion extracts was assessed by applying carrageenan sub plantar test to Wistar rats, but acetic acid test was used to study analgesic effect. Results The polyphenols content and antioxidant activities of onion extracts changed significantly (p < 0.05) with variety and increased (p < 0.05) with concentration. Total phenolic (range 58.03–67.43%), Total Reducing power (range 0.27–0.94%), DPPH radical scavenging (range 37.33–202.08%) were higher in Violet of galmi extracts, while Ferrous ion chelating power (range 23.15–97.42%) was higher in Goudami extract. The two varieties of onion extracts reduce (p < 0.05) rat paw edema in a dose dependent manner compared with negative control group. Violet of Galmi variety (1.5 mg/kg) reaches 100% of inhibition after 2 h whereas the Goudami variety (1.5 mg/kg) reaches 100% of inhibition after 3 h. In terms of analgesic activity, aqueous extract of the two varieties of onion exhibited analgesic effect characterized by reduction in writhes. Analgesic activity increased significantly (p < 0.05) with concentration of onion but Violet of galmi extracts has also shown more efficacy than Goudami extracts. Conclusion The present findings suggest that aqueous extract of violet of galmi and Goudami bulb onions have antioxidant activity and protective effects against inflammation and pain.

scholarly journals Role of Dried Fruits ofCarissa carandasas Anti-Inflammatory Agents and the Analysis of Phytochemical Constituents by GC-MS

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
N. Anupama ◽  
G. Madhumitha ◽  
K. S. Rajesh
Keyword(s):  
Methanol Extract ◽  
Inflammatory Diseases ◽  
Control Group ◽  
Significant Activity ◽  
Dependent Manner ◽  
Paw Edema ◽  
Dried Fruits ◽  
Carissa Carandas ◽  
Dried Fruit ◽  
Anti Inflammatory

Inflammation plays an important role in various diseases with high prevalence within populations such as rheumatoid arthritis, ulcer, atherosclerosis, and asthma. Many drugs are available in the market for inflammatory diseases. They exhibit several unwanted side effects to humans. Therefore, alternative treatments with safer compounds are needed.Carissa carandasplant is used in traditional medicinal system for its various diseases curing property. In the present study, we examined the anti-inflammatory effects of dried fruit methanol extract on carrageenan-induced hind paw edema in rats.C. carandaswas defatted with petroleum ether, followed by methanol extraction. The methanol extracts of the dried fruits ofCarissa carandaswere given orally to the experimental rats caused significant activity (P≤0.05) when compared with the control group. The maximum inhibition of paw edema was found to be in Group V, that is, 76.12% with inhibition of paw volume in a dose-dependent manner. The anti-inflammatory activity of the methanol extract of the dried fruits shows that the presence of potential constituents present in this extract may provide assistance in the drug discovery process. The phytochemical compounds of the extract were screened by GC-MS analysis and it was found that 11 compounds are present in methanol extract of dried fruits ofCarissa carandas.

Anti-inflammatory activity of Elaeagnus angustifolia fruit extract on rat paw edema

2017 ◽  
Vol 28 (4) ◽  
Author(s):  
Manijeh Motevalian ◽  
Mehdi Shiri ◽  
Saeedeh Shiri ◽  
Zahra Shiri ◽  
Hadi Shiri
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Dependent Manner ◽  
Rat Paw Edema ◽  
Chronic Study ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Dose Dependent ◽  
Inflammatory Effect

AbstractBackground:TheMethods:The acute and chronic anti-inflammatory effects ofResults:In the acute anti-inflammatory study, the extract showed a significant anti-inflammatory effect in a dose-dependent manner. The results of 1000 mg/kg of the extract was significantly different compared with the negative control group (p<0.05) and was comparable to sodium salicylate (p<0.05). Results from the chronic study suggested thatConclusions:Based on our findings, the

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