Mechanical and In-Vitro Cell Compatibility Properties of Silk-Elastinlike Protein-Based Biomaterial

2010 ◽  
Author(s):  
Weibing Teng ◽  
Yiding Huang ◽  
Joseph Cappello ◽  
Xiaoyi Wu
Keyword(s):  
Mechanical Property ◽  
Cell Culture ◽  
Mechanical Strength ◽  
Genetically Engineered ◽  
Load Bearing ◽  
Cell Compatibility ◽  
Chemical Treatments ◽  
Good Biocompatibility ◽  
Physical And Chemical

A series of genetically engineered recombinant silk-elastinlike proteins (SELPs) have been produced by combining polypeptide sequences derived from native silk of superior mechanical strength and elastin that is extremely durable and resilient. They have displayed a set of outstanding properties such as good biocompatibility and controllable biodegradation rates. In the study, we characterized the mechanical property of genetically engineered, recombinant silk-elastinlike protein copolymer, SELP-47K, under physical and chemical treatments. The biocompatibility of the SELP-47K was also evaluated by cell culture. The ultimate goal of this study is to explore the potential of SELPs for applications in the engineering of load-bearing tissues such as arteries.

Improvement of Bioactivity of Collagen/Alginate Scaffolds by Hydroxyapatite for Tissue Engineering Cartilage

2008 ◽  
Vol 396-398 ◽  
pp. 445-448 ◽  
Author(s):  
J. Sun ◽  
R. Wang ◽  
L. Zheng ◽  
Yan Fei Tan ◽  
Yu Mei Xiao ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Tissue Engineering ◽  
Cell Culture ◽  
Composite Film ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
The Poor ◽  
Pure Collagen ◽  
Good Biocompatibility

With good biocompatibility, collagen is often used in cartilage tissue engineering. Collagen/alginate composite was hoped to improve the poor mechanical property of pure collagen but the biocompatibity was decreased. In this study, hydroxyapatite (HA) particles were used to get collagen/alginate/HA (CAHA) composite film to enhance the bioactivity properties. The bioactivity of the composite was investigated by in vitro co-culture with chondrocytes. During the 6-day cell culture in vitro, the composite showed a significant improvement in promoting proliferation and maintaining morphology/phenotype of the chondrocytes over collagen/alginate composite by MTT, SEM, fluorescent and immunohistochemical assays. Cytocompatibility and cytoviablility of CAHA even come up to that of collagen film alone. The results indicated that the composite film may provide an appropriate environment for the proliferation and maintaining the morphology and phenotype of chondrocytes and have a potential clinical application in the cartilage tissue engineering field.

Secondary Structures and Mechanical Properties of Biomimetic Protein Polymers

2009 ◽  
Author(s):  
Weibing Teng ◽  
Joseph Cappello ◽  
Xiaoyi Wu
Keyword(s):  
Mechanical Properties ◽  
Mechanical Strength ◽  
Mechanical Behavior ◽  
Half Life ◽  
Secondary Structures ◽  
Load Bearing ◽  
Protein Polymers ◽  
Human Arteries ◽  
Good Biocompatibility ◽  
Polypeptide Sequences

Silk may possess superior mechanical strength while its resilience is very poor. In contrast, elastin in human arteries is very soft but extremely durable with an estimated half-life of 70 years. By combing polypeptide sequences derived from native silk and elastin, we have produced a series of silk-elastin-like proteins (SELPs), which have displayed a set of outstanding properties such as good biocompatibility and controllable biodegradation rates [1]. In this study, we will examine the crystallization of the silk-like blocks and the crosslinking of the elastin-like blocks, as well as their influences on the mechanical behavior of SELPs. The ultimate goal of this study is to explore the potential of SELPs for applications in the engineering of load-bearing tissues such as arteries.

Sol-Gel Synthesis and In Vitro Cell Compatibility Analysis of Silicate-Containing Biodegradable Hybrid Gels

2007 ◽  
Vol 361-363 ◽  
pp. 447-450 ◽  
Author(s):  
Kanji Tsuru ◽  
Z. Robertson ◽  
B. Annaz ◽  
Iain R. Gibson ◽  
Serena Best ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Differentiation ◽  
Tissue Regeneration ◽  
Sol Gel ◽  
Mixing Ratio ◽  
Hybrid Gels ◽  
Cell Compatibility ◽  
Compatibility Analysis ◽  
Sol Gel Synthesis

Novel hybrid gels in the system gelatin-GPTMS-TEOS were prepared via a sol-gel route, and their ability to release Si(IV) was examined using MG63 osteoblast-like cell culture. The amount of Si released and the release rate were controllable by changing the mixing ratio of GPTMS and TEOS. In addition, the hybrids had biocompatible surfaces. It is expected that the hybrids will be utilized for the investigation of the effect of Si on cell differentiation and tissue regeneration.

scholarly journals Synthesis and Properties of Gelatin Methacryloyl (GelMA) Hydrogels and Their Recent Applications in Load-Bearing Tissue

Polymers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1290 ◽  
Author(s):  
Mingyue Sun ◽  
Xiaoting Sun ◽  
Ziyuan Wang ◽  
Shuyu Guo ◽  
Guangjiao Yu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Physicochemical Properties ◽  
Uv Light ◽  
Cutting Edge ◽  
Load Bearing ◽  
Good Biocompatibility ◽  
Biomedical Field ◽  
And Cartilage ◽  
Typical Method

Photocrosslinked gelatin methacryloyl (GelMA) hydrogels have attracted great concern in the biomedical field because of their good biocompatibility and tunable physicochemical properties. Herein, different approaches to synthesize GelMA were introduced, especially, the typical method using UV light to crosslink the gelatin-methacrylic anhydride (MA) precursor was introduced in detail. In addition, the traditional and cutting-edge technologies to characterize the properties of GelMA hydrogels and GelMA prepolymer were also overviewed and compared. Furthermore, the applications of GelMA hydrogels in cell culture and tissue engineering especially in the load-bearing tissue (bone and cartilage) were summarized, followed by concluding remarks.

scholarly journals Preparation and Bioactivity Properties of a Novel Composite Membrane of Fructose Mediatedβ-Tricalcium Pyrophosphate/(Polyethylene Glycol/Chitosan) for Guided Tissue Regeneration

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Jian-Wen Wang ◽  
Min-Hsiung Hon ◽  
Yi-Ming Kuo ◽  
Mei-Hui Chung
Keyword(s):  
Mechanical Property ◽  
Tissue Regeneration ◽  
Composite Membrane ◽  
Degradation Rate ◽  
Composite Membranes ◽  
Guided Tissue Regeneration ◽  
Glycol Chitosan ◽  
Cell Compatibility ◽  
Suitable Material

A novel composite membrane ofβ-tricalcium pyrophosphate (β-TCP) and fructose- (F-) mediated chitosan/poly(ethylene glycol) (CS/PEG) was prepared by thermally induced phase separation technique. The prepared composite membranes were characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD). The mechanical property, swelling, degradation, and cytotoxicity of the composite membranes were evaluated in vitro with respect to its potential for use as biodegradable guided tissue regeneration (GTR) membrane. In vitro degradation tests showed the composite membrane with a controllable degradation rate when changing theβ-TCP content. The incorporation ofβ-TCP granules also caused a significant enhancement of tensile strength. Whenβ-TCP content is controlled to 50 wt%, homogeneous composite membranes with well mechanical property and enzymatic degradation rate can be obtained. Cytotoxicity assay demonstrates that the composite membranes were nontoxic and had very good cell compatibility. Most importantly, the release of calcium ions and glucosamine from the composite membranes was proved to increase the cell proliferation of NIH3T3. The results of this study have indicated that this novel F-β-TCP/CS/PEG composite can be a suitable material for GTR applications.

scholarly journals Nanocomposite bioink exploits dynamic covalent bonds between nanoparticles and polysaccharides for precision bioprinting

2019 ◽  
Author(s):  
Mihyun Lee ◽  
Kraun Bae ◽  
Clara Levinson ◽  
Marcy Zenobi-Wong
Keyword(s):  
Mechanical Strength ◽  
Rheological Properties ◽  
Yield Stress ◽  
Recent Progress ◽  
Mechanical Stability ◽  
Covalent Bonds ◽  
Good Biocompatibility

AbstractThe field of bioprinting has made significant recent progress towards engineering tissues with increasing complexity and functionality. It remains challenging, however, to develop bioinks with optimal biocompatibility and good printing fidelity. Here, we demonstrate enhanced printability of a polymer-based bioink based on dynamic covalent linkages between nanoparticles (NPs) and polymers, which retains good biocompatibility. Amine-presenting silica NPs (ca. 45 nm) were added to a polymeric ink containing oxidized alginate (OxA). The formation of reversible imine bonds between amines on the NPs and aldehydes of OxA lead to significantly improved rheological properties and high printing fidelity. In particular, the yield stress increased with increasing amounts of NPs (14.5 Pa without NPs, 79 Pa with 2 wt% NPs). In addition, the presence of dynamic covalent linkages in the gel provided improved mechanical stability over 7 days compared to ionically crosslinked gels. The nanocomposite ink retained high printability and mechanical strength, resulting in generation of centimetre-scale porous constructs and an ear structure with overhangs and high structural fidelity. Furthermore, the nanocomposite ink supported both in vitro and in vivo maturation of bioprinted gels containing chondrocytes. This approach based on simple oxidation can be applied to any polysaccharide, thus the widely applicability of the method is expected to advance the field towards the goal of precision bioprinting.

Mechanical Property and Cell Compatibility of Silk/PLGA Hybrid Scaffold; In Vitro Study

Polymer Korea ◽  
2011 ◽  
Vol 35 (3) ◽  
pp. 189-195 ◽  
Author(s):  
Yi Seeul Song ◽  
Han Na Yoo ◽  
Shin Eum ◽  
On You Kim ◽  
Suk Chul Yoo ◽  
...  
Keyword(s):  
Mechanical Property ◽  
In Vitro Study ◽  
Vitro Study ◽  
Hybrid Scaffold ◽  
Cell Compatibility

Properties of Calcium Phosfate Cement with Addition of Dispersant and Hydrogel

2012 ◽  
Vol 727-728 ◽  
pp. 1170-1174 ◽  
Author(s):  
J.M. Fernandes ◽  
W.T. Coelho ◽  
Mônica Beatriz Thürmer ◽  
Rafaela Silveira Vieira ◽  
Luis Alberto Santos
Keyword(s):  
Mechanical Properties ◽  
Calcium Phosphate ◽  
Mechanical Strength ◽  
Sodium Alginate ◽  
In Vitro Test ◽  
Polymeric Additives ◽  
Calcium Phosphate Bone Cement ◽  
Vitro Test ◽  
Good Biocompatibility

The calcium phosphate cements (CPCs) have attracted great interest for use in orthopedics and dentistry as replacements for damaged parts of the skeletal system,showing good biocompatibility and osseointegration. These characteristics allow its use as a bone graft.Several studies in literature have shown that the addition of polymeric additives has a strong influence on the mechanical properties of cement. The low mechanical strength is the main impediment to a broader use of calcium phosphate bone cement (CPCs) as implant material. The aim of this work was evaluate the strength of a CPC based on α-tricalcium phosphate, with polymeric additions. CPC was synthesized and sodium alginate were added (1%, 2% and 3% by weight) and ammonium polyacrylate (3%; dispersant) in aqueous solution. Specimens were molded and evaluated for density, pH, porosity, in vitro test (Simulated Body Fluid),crystalline phases and mechanical strength. The results show the increase of the mechanical properties of cement when added with sodium alginate and dispersant.

An Evaluation of Sterilisation Processes

2008 ◽  
Vol 36 (5) ◽  
pp. 585-590 ◽  
Author(s):  
Dariusz Śladowski ◽  
Iwona Grabska-Liberek ◽  
Joanna Olkowska-Truchanowicz ◽  
Kamil Lipski ◽  
Grzegorz Gut
Keyword(s):  
Cell Culture ◽  
Ethylene Oxide ◽  
Chemical Properties ◽  
Test Method ◽  
Test Material ◽  
Adherent Cell ◽  
In Vitro Cell Culture ◽  
Physical And Chemical ◽  
And Storage

A sterile environment is one of the basic elements of in vitro cell culture. When choosing an appropriate sterilisation method, the possibility that the physical and chemical properties of the sterilised material could be altered by the sterilisation process itself, should be considered. Avoiding any potential problems of toxicity arising as a consequence of the sterilisation process is essential, not only in in vitro cell culture procedures, but especially in the case of the sterilisation of medical devices which come into contact with human tissue (e.g. catheters, surgical tools, and containers used for transplant preparation and storage). As it is not possible to predict the potential effects of every combination of test material and sterilisation process, we have designed a simple test, which can be easily performed to ensure the absence of cytotoxicity. The test involves the culturing of a non-adherent cell line in direct contact with the test material, in micro-wells attached to the surface of the test device. By using this novel test method, three sterilisation procedures were compared for each material. The results indicated that, neither ionising irradiation nor ethylene oxide left toxic residues on the surface of polystyrene; and that, in the case of steel, neither steam sterilisation nor ethylene oxide left toxic residues on the metal. The cold plasma system, which left toxic residues on the surface of both materials, required a post-sterilisation period of 24 hours in the case of steel, and 10 days in the case of polystyrene, in order to eliminate toxic residues prior to their use.

Preliminary Study of the Application of PET Knitted Fabrics in Artificial Bone Scaffold

2012 ◽  
Vol 184-185 ◽  
pp. 1501-1504 ◽  
Author(s):  
Ching Wen Lou ◽  
Mei Hui Li ◽  
Wen Cheng Chen ◽  
Jin Jia Hu ◽  
Chao Tsang Lu ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Cell Culture ◽  
Mechanical Strength ◽  
Polyethylene Terephthalate ◽  
Cell Attachment ◽  
Bone Scaffold ◽  
Artificial Bone ◽  
Knitted Fabrics ◽  
Preliminary Study ◽  
Plied Yarn

Having good mechanical strength, biocompatibility, and workability, polyethylene terephthalate (PET) is often used as a biomaterial. In this study, PET filaments with various deniers are made into plied yarn with various coefficients of twist. The plied yarn is then made into PET knitted fabrics. Mechanical property tests are performed to determine the differences among the various PET knitted fabrics. Finally, by using cell culture, the PET knitted fabrics are analyzed and evaluated with their cell attachment.

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