scholarly journals Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts fromIn VivoandIn VitroGrownTrifolium pratenseL. (Red Clover)

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Arash Khorasani Esmaeili ◽  
Rosna Mat Taha ◽  
Sadegh Mohajer ◽  
Behrooz Banisalam
Keyword(s):  
Antioxidant Activity ◽  
Callus Tissue ◽  
Radical Scavenging ◽  
Red Clover ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Chloroform Fraction ◽  
Flavonoid Content

In the present study the extracts ofin vivoandin vitrogrown plants as well as callus tissue of red clover were tested for their antioxidant activities, using different extraction solvent and different antioxidant assays. The total flavonoid and phenolic contents as well as extraction yield of the extracts were also investigated to determine their correlation with the antioxidant activity of the extracts. Among all the tested extracts the highest amounts of total phenolic and total flavonoids content were found in methanol extract ofin vivogrown plants. The antioxidant activity of tested samples followed the orderin vivoplant extract > callus extract >in vitroextract. The highest reducing power, 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging, and chelating power were found in methanol extracts ofin vivogrown red clover, while the chloroform fraction ofin vivogrown plants showed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, superoxide anion radical scavenging and hydrogen peroxide scavenging compared to the other tested extracts. A significant correlation was found between the antioxidant activity of extracts and their total phenolic and total flavonoid content. According to the findings, the extract ofin vitroculture of red clover especially the callus tissue possesses a comparable antioxidant activity to thein vivocultured plants’ extract.

scholarly journals EVALUATION OF IN VITRO ANTIOXIDANT AND α-AMYLASE INHIBITORY ACTIVITY OF PHYLLANTHUS INDOFISCHERI BENNET

2016 ◽  
Vol 8 (11) ◽  
pp. 131 ◽  
Author(s):  
Kalpana S ◽  
Ramakrushna B. ◽  
Anitha S.
Keyword(s):  
Inhibitory Activity ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ic50 Values

Objective: The present study evaluates the antioxidant and α-amylase inhibitory activity of leaf and bark extracts of Phyllanthus indofischeri with methanol and water as solvents. In addition to this, the total phenolic content and total flavonoid content was determined.Methods: The total phenolic and total flavonoid content of the extracts was determined by folin ciocaletus reagent method and aluminium chloride colorimetric method respectively. The antioxidant and α-amylase inhibitory activity were measured by various assays, including α, α-diphenyl-ẞ-dipicryl-hydrazyl (DPPH) free radical scavenging, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) radical scavenging, superoxide radical scavenging, total antioxidant capacity by phosphomolybdate method and porcine pancreatic α-amylase inhibitory assay. The IC50 values were calculated and compared with standards such as gallic acid, ascorbic acid and α-acarbose.Results: The results illustrated that all the extracts of Phyllanthus indofischeri exhibit significant antioxidant and α-amylase inhibitory activity. Among the extracts, methanolic leaf extract showed high levels of activity followed by bark water extract.Conclusion: Phyllanthus indofischeri extracts had shown antioxidant and α-amylase inhibitory activity. On the basis of these results, Phyllanthus indofischeri can be used as a natural antioxidant and hypoglycemic agent against various disorders related to oxidative stress; and the isolation of bioactive compounds was warranted. 

PHYTOCHEMICAL SCREENING, TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF DIFFERENT PARTS OF Melastoma malabathricum

2015 ◽  
Vol 77 (2) ◽  
Author(s):  
Suleiman Danladi ◽  
Amirah Wan-Azemin ◽  
Yahaya Najib Sani ◽  
Khamsah Suryati Mohd ◽  
Mahadeva Rao US ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Phenolic Contents ◽  
Melastoma Malabathricum ◽  
Different Parts

Melastoma malabathricum Linn. commonly known as ‘senduduk’ in Malaysia, belongs to Melastomataceae family. The study was carried out to screen the phytochemicals present in methanol extract of leaf, flower, fruit, and stem and to evaluate the antioxidant activity, total flavonoid and total phenolic contents of these different parts. Phytochemical screening showed that all parts of this plant contained tannins, steroids, phenols and flavonoids. The flower extract was found to have highest total phenolic whereas the leaf demonstrated the highest flavonoid content followed by flower. The results of antioxidant activity from the DPPH assay showed that the flower has the highest radical scavenging activity comparable to quercetin standard while stem showed the lowest activity. The higher content of total phenolics, flavonoids and antioxidant capacity of flower suggests the possibility of its incorporation and exclusion of stem in M. malabathricum preparations for development of newer effective drugs.

scholarly journals PHYTOCHEMICAL PROFILING, ASSESSMENT OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF ETHNOMEDICINAL PLANT, MEYNA SPINOSA FROM ASSAM

2019 ◽  
pp. 61-63
Author(s):  
REKHA BORA ◽  
SEEMA KHAKHALARY ◽  
TAPAN DUTTA
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Radical Scavenging Assay

Objective: The present study qualitative phytoconstituents examine the total phenol, total flavonoid content (TFC), and antioxidant efficiencies traditionally used plant, Meyna spinosa. Methods: Chemical profiling, estimation of total phenolic content (TPC), TFC, and antioxidant activity of ethanol extracts of M. spinosa have performed by applying standard protocols. Antioxidant activity of leaf and stem was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. TPC and TFC of the plant were assessed using Folin–Ciocalteu colorimetric and aluminum colorimetric assay, respectively. Results: The findings of the study exhibit that ethanol extract of M. spinosa is proved to be the presence of phytoconstituents (7/9) such as alkaloids, terpenoids, saponins, tannins, phytosterols, flavonoids, and phenolic compounds, while carbohydrate, fixed oils, and fats are unavailable. In addition, phenolic compositions of ethanol extract of leaf and stem; 93.21±2.93 and 54.33±0.69 mg gallic acid equivalents/g extract, respectively; TFCs of leaf and stem have recorded as 61.55±1.21 and 37.55±1.28 mg quercetin equivalents/g extract, respectively. Antioxidant efficiency of both leaf and stem is tested using DPPH radical scavenging assay as IC50 20.68±0.32 and 50.99±0.56 μg/ml, respectively. Conclusion: From the above results, it has concluded that the ethanol extract of the M. spinosa leaves and stems seizes rich phytoconstituents which can be applied in food technology, drug industries, ethnopharmacological fields, etc., for the development of healthiness and to battle against negative health consequences.

scholarly journals Phenolic content and antioxidant activity of Echinocystis lobata (Mich.) Torr. ET Gray (Cucurbitaceae)

10.5219/1579 ◽  
2021 ◽  
Vol 15 ◽  
pp. 784-791
Author(s):  
Yulia Vinogradova ◽  
Olga Shelepova ◽  
Olena Vergun ◽  
Olga Grygorieva ◽  
Jan Brindza
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Echinocystis Lobata

Echinocystis lobata (Mich.) Torr. et Gray extracts were investigated for their bioactive compounds and their potential for natural antioxidant properties. In vitro antioxidant activity of methanol, ethanol, and water extracts were evaluated by 1,1-diphenyl-picrylhydrazyl (DPPH) radical scavenging activity. Total phenolic content (TPC) and total flavonoid content (TFC) of fruits and seeds were determined. TPC is a fairly constant value and varies weakly both among the different organs of Echinocystis lobata and among the geographical locations of the populations studied. Like other invasive species, Echinocystis lobata is superior to many other plants in terms of the total amount of flavonoids contained in its fruits and seeds. The dried fruits contain more flavonoids than the seeds. Seeds from Slovak populations have higher flavonoid content than those from Russian populations. The results demonstrated that Echinocystis lobata can be a good source of natural antioxidants and have a great potential for commercialization, especially in the pharmaceutical industry.

scholarly journals ISOLATION OF QUERCETIN FROM FLOWER PETALS, ESTIMATION OF TOTAL PHENOLIC, TOTAL FLAVONOID AND ANTIOXIDANT ACTIVITY OF THE DIFFERENT PARTS OF RHODODENDRON ARBOREUM SMITH

2015 ◽  
Vol 12 (12) ◽  
pp. 34-40 ◽  
Author(s):  
Laxman Bhandari ◽  
Meena Rajbhandari
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Acetone Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Different Parts

The flowers of Rhododendron arboreum Smith is a source of polyphenolic compounds. A flavonol, quercetin, was isolated from ethyl acetate fraction of the methanol extract of flower petals of R. arboreum by repeated Sephadex LH-20 column chromatography. Isolated quercetin was characterized by comparing melting point, Rf values, UV and IR spectra with authentic quercetin. The isolated quercetin was used as a standard for the estimation of total flavonoids.  Total phenolic and total flavonoid content in different parts of R. arboreum was carried out spectrophotometrically using Folin-Ciocalteu reagent and Aluminium chloride reagent respectively. Gallic acid and quercetin were used as standard for the construction of calibration curve of phenolic and flavonoid respectively. The results showed that the highest total phenolic content was detected in the 70% acetone extract of the flowers (600 mg GAE/g extract) and petals (600 mg GAE/g extract) and the lowest amount was detected in methanol extract of stem (188 mg GAE/g extract). Similarly, the highest total flavonoid was detected in the 70% acetone extract of the twigs (170 mg QE/g extract) and the lowest amount was detected in the methanol extract of stem (45 mg QE/g extract).  The antioxidant activity of the methanol extracts obtained from different parts of R. arboreum was determined by 2, 2- diphenyl-1-picryl hydrazyl (DPPH) assay and the radical scavenging activity (IC50) was calculated. The highest free radical scavenging effect was observed in leaves with IC50=8.34 ?g/ml and lowest was observed in stem with IC50=67.83 ?g/ml. The IC50 values, total phenolic and total flavonoid content (correlation coefficient R2= 0.923 for phenolic, R2= 0.965 for flavonoid) were correlated which showed strong correlation indicating that the major components responsible for antioxidant activity is phenolics. The highest the phenolic content, the lowest the IC50 value observed. The result indicated that R. arboreum is a rich source of high value polyphenols as natural antioxidant to use in preventive medicine as well as in food and pharmaceutical industry.Scientific World, Vol. 12, No. 12, September 2014, page 34-40         

Tylophora hirsuta (Wall.) extracts ameliorates diabetes associated with inflammation in alloxan induced diabetic rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Faisal Razzaque ◽  
Ali Sharif ◽  
Bushra Akhtar ◽  
Humaira Majeed Khan ◽  
Muhammad Furqan Akhtar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Diabetic Rats ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Polyphenolic Composition ◽  
Anti Inflammatory

Background: Tylophora hirsuta Wall. has long been used as traditional medicine for the treatment of diabetes. Current study is designed to evaluate the antidiabetic and anti-inflammatory activity of different extracts of aerial parts of Tylophora hirsuta. Methods: Sequential maceration was conducted to obtain extracts. Total phenolic contents were determined by FolinCiocalteau method. The antioxidant activity was assessed by DPPH free radical scavenging assay. The extracts were tested for its inhibitory activity against α-amylase in-vitro. In-vivo anti diabetic assay was conducted using alloxan induced diabetic model and OGTT was conducted on normal rats. ELISA was used to determine the proinflammatory cytokines (TNF-α and IL-6). Polyphenolic composition of the extract was analyzed using a HPLC system. Results: Aqueous extract exhibited highest total phenolic contents (985.24± 3.82 mg GAE/100 g DW), antioxidant activity (IC50 = 786.70 ± 5.23 Conclusion: These results showed that Tylophora hirsuta possess strong antidiabetic and anti-inflammatory potentials and justify its folklore use for the management of diabetes.

scholarly journals Potential of corncobs (Zea mays) fraction as tyrosinase inhibitor and natural antioxidant in vitro

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 67-73
Author(s):  
I.K. Dewi ◽  
S. Pramono ◽  
A. Rohman ◽  
R. Matien
Keyword(s):  
Antioxidant Activity ◽  
Zea Mays ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Inhibition Test ◽  
Total Phenolic ◽  
Chloroform Fraction ◽  
Tyrosinase Enzyme

Corncobs (Zea mays) are beneficial to human health as they contain tyrosinase inhibitors and natural antioxidants, but they are not used as they are considered as waste. This research evaluated the inhibition test towards tyrosinase enzyme and antioxidant activity of corncob fraction using in-vitro DPPH method and its correlation to phenolic and flavonoids. Corncob fraction was extracted using the maceration method applying 70% ethanol solvent. The ethanol extract of corncob was suspended by water and then partitioned with chloroform, ethyl acetate, and aquadest to produce three fractions (chloroform, ethyl acetate, and aquadest fractions). These fractions were analyzed through the tyrosinase inhibition test, applying in vitro tyrosinase enzyme inhibition and antioxidant activity using radical scavenging test DPPH (2 2,2-diphenyl-1-picrylhydrazyl). Meanwhile, the total phenolic and flavonoids content tests were determined spectroscopically. The results showed ethyl acetate fraction had the highest tyrosinase activity with IC50 values of 185.76 µg/mL, followed by the aquadest fraction (IC50 676.44µg/ml) and the chloroform fraction (IC50 709.26 µg/mL). The antioxidant activity using DPPH radical scavenging method exhibited that ethyl acetate fraction had the highest antioxidant activity with IC50 of 25.79 µg/mL followed by the chloroform fraction (IC50 of 29.15 µg/mL) and the aquadest fraction (IC50 of 32.41 µg/mL). The total phenolic content of the corncob fraction ranged between 1.73 to 7.43% (w/w) gallic acid equivalents (GAE), while the entire flavonoid content ranged between 0.01 to 1.34% (w/ w) quercetin equivalent (QE). The tyrosinase activity and antioxidants of the corncob fractions correlated with the total phenolic and flavonoid contents.

scholarly journals Screening of Bio-active Pigments, Antioxidant Activity, Total Phenolic and Flavonoid Content of Some Economically Important Medicinal Plants for Ethno-botanical Uses

2021 ◽  
Vol 9 (10) ◽  
pp. 1767-1774
Author(s):  
Md. Abdul Hakim ◽  
Mohammed Arif Sadik Polash ◽  
Md. Ashrafuzzaman ◽  
Md. Solaiman Ali Fakir
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Chlorophyll A ◽  
Ricinus Communis ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Chlorophyll B ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

An experiment was conducted to determinate the phytochemical properties (chlorophyll-a, chlorophyll-b, carotenoids, antioxidant activity, total phenolic and flavonoid content) in ten medicinal plants namely Syal Kata (Argemone mexicana L.), Akanda (Calotropis gigantea (L.) Dryand.), Dumur (Ficus carica L.), Chalmugra (Gynocardia odorata R.Br.), Kata kachu (Lasia spinosa (L.) Thwaites), Shetodrone (Leucas aspera (Wild.)), Khona (Oroxylum indicum (L.) Kurz), Reri (Ricinus communis L.), Ghat kachu (Typhonium trilobatum (L.) Schott) and Bazna (Zanthoxylum rhetsa DC.) for ethno-botanical uses. The young fresh leaves were harvested and were subjected to methanolic (95%) extract. Total phenolic contents were analysed by using Folin - Ciocalteau method where gallic acid was used as standard. Total phenolic content varied from 94.53 mg GAE/100 g FW (C. gigantea) to 484.88 mg GAE/100 g FW (L. aspera). Total flavonoid contents were performed by using Quercetin as standard. Total flavonoid content varied from 137.25 mg QUE /100 g FW (C. gigantea) to 334.27 mg QUE/100 g FW (G. odorata). Antioxidant activity of these extracts was performed by using DPPH free radical scavenging assay. Total anti-oxidant capacity varied from IC50 value 35.37 mg/mL (A. mexicana) to 90.47 mg/mL (F. carica) where ascorbic acid is used as standard. Results indicated that among the ten medicinal plants phenolics rich in L. aspera, flavonoids rich in G. odorata, antioxidant activity rich in A. mexicana. Z. rhetsa leaf rich in chlorophyll-a content (311.67 mg/100 g FW), L. aspera leaf rich in chlorophyll-b content (157 mg/100 g FW), Z. rhetsa rich in chlorophyll- (a+b) content as 439 mg/100 g FW, A. mexicana leaf rich in carotenoids content as 96 mg/100 g FW.

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