scholarly journals Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Haican Liu ◽  
Lulu Lian ◽  
Yi Jiang ◽  
Mingxiang Huang ◽  
Yunhong Tan ◽  
...  

Pulmonary diseases caused by nontuberculousmycobacteria(NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces in China, in order to preliminarily provide some basic scientific data in the different species and distribution of NTM related to pulmonary disease in China. A total of 523 clinical isolates from patients with tuberculosis (TB) diagnosed clinically from 2005 to 2012 were identified to the species using conventional and molecular methods, including multilocus PCR,rpoBandhsp65PCR-PRA,hsp65,rpoB, and16S-23Sinternal transcribed spacer region sequencing. The isolates were identified into 3 bacterium genera, including NTM,Gordonia bronchialis,andNocardia farcinica, and, for the 488 NTM isolates, 27 species were identified. For all the 27 species of NTM which were found to cause pulmonary infections in humans, the most prevalent species wasM. intracellulare, followed byM. aviumandM. abscessus. And seven other species were for the first time identified in patients with TB in China. NTM species identification is very important for distinguishing between tuberculosis and NTM pulmonary diseases, and the species diversity drives the creation of diverse and integrated identification methods with higher accuracy and efficacy.

2015 ◽  
Vol 19 (6) ◽  
pp. 670-671 ◽  
Author(s):  
Sheng Bi ◽  
Kai-jin Xu ◽  
Zhong-kang Ji ◽  
Bei-wen Zheng ◽  
Ji-fang Sheng

2021 ◽  
Vol 65 (5) ◽  
Author(s):  
Dae Hun Kim ◽  
Su-Young Kim ◽  
Hee Jae Huh ◽  
Nam Yong Lee ◽  
Won-Jung Koh ◽  
...  

ABSTRACT We evaluated the in vitro activity of rifamycin derivatives, including rifampin, rifapentine, rifaximin, and rifabutin, against clinical nontuberculous mycobacteria (NTM) isolates. Of the rifamycin derivatives, rifabutin showed the lowest MICs against all NTM species, including Mycobacterium avium complex, M. abscessus, and M. kansasii. Rifabutin also had effective in vitro activity against macrolide- and aminoglycoside-resistant NTM isolates. Rifabutin could be worth considering as a therapeutic option for NTM disease, particularly drug-resistant disease.


Mycotaxon ◽  
2020 ◽  
Vol 135 (4) ◽  
pp. 845-852
Author(s):  
Junaid Khan ◽  
Hassan Sher ◽  
Shah Hussain ◽  
Abdul Nasir Khalid

Hericium cirrhatum, a widespread but locally rare tooth fungus is reported for the first time from Pakistan. This species is characterized by white to cream semicircular basidiomata (usually arranged in tiers) with a granular to slightly spiny sterile upper surface, a fertile under surface with 10–15 mm long pointed spines, and ellipsoid to subglobose basidiospores. Morphological characters and DNA sequence analyses inferred from the nuclear ribosomal internal transcribed spacer region (nrITS) confirm the identity of the taxon.


Zootaxa ◽  
2006 ◽  
Vol 1344 (1) ◽  
pp. 23
Author(s):  
ANDRÉS BASELGA ◽  
FRANCISCO NOVOA

Mature larva of Hydrothassa fairmairei (Brisout) is described and illustrated for the first time, based on specimens collected on Ranunculus spp. (Ranunculaceae) in Spain. A key to known larvae of genus Hydrothassa Thomson is provided. Diagnostic characters for the identification of species within this genus are number and size of abdominal dorsal tubercles, size of prothoracical setae and number of setae of anterior dorsal mesoand metathoracical tubercles. The larva of H. fairmairei is similar to that of H. marginella (Linné), but is clearly separated by the number of setae of dorsal anterior tubercle of mesoand metathorax. Notes on distribution and host plants are included.


Zootaxa ◽  
2008 ◽  
Vol 1745 (1) ◽  
pp. 47
Author(s):  
ANDRÉS BASELGA

Mature larva of Gonioctena (Spartoxena) pseudogobanzi Kippenberg, 2001 is described and illustrated for the first time, based on specimens collected on Genista umbellata (L’Hér.) Dum. Cours. [Fabaceae] in southeastern Spain. Diagnostic characters for the identification of species within the subgenus Sparoxena Motschulsky, 1860 are number of dorsal tubercles of abdominal segments, shape of tarsal claw, shape of labrum and disposition of microtrichia of epipharynx. A key to known larvae of subgenus Spartoxena is provided, along with figures illustrating the diagnostic characters. Notes on the distribution and host plant of G. pseudogobanzi are included.


1981 ◽  
Vol 44 (11) ◽  
pp. 877-880 ◽  
Author(s):  
DANIEL Y. C. FUNG ◽  
NELSON A. COX

A symposium at the 1980 Annual Meeting of IAMFES permitted manufacturers to explain their diagnostic kits and food microbiologists to relate their experiences with the kits. Discussions between manufacturers and users resulted in the identification of means for improving systems used to rapidly identify pathogens and nonpathogens in foods. The general conclusions reached were: (a) food microbiologists continue to seek rapid methods to identify isolates from foods, and commercial diagnostic kits are valuable for this purpose, (b) current identification methods established by diagnostic kit manufacturers, although useful for clinical isolates, need improvements when applied to food isolates, (c) the ideal situation would be for manufacturers to generate specific identification methods for food isolates, (d) comparative analytical studies should be made before adapting commercial systems to routine use and (e) under special conditions, researchers can expand the use of existing diagnostic kits to purposes not originally envisioned by the manufacturers.


Water ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 363 ◽  
Author(s):  
Neila Annabi-Trabelsi ◽  
Gamal El-Shabrawy ◽  
Mohamed E. Goher ◽  
Madhavapeddi N. V. Subrahmanyam ◽  
Yousef Al-Enezi ◽  
...  

The copepod assemblages and abiotic parameters were investigated at 11 stations in a large coastal lake (Lake Manzalah, Nile Delta) from 2009–2010 in order to verify any impacts of eutrophication and salinity on the copepod species composition. The environmental conditions and the copepod assemblages appeared to have changed in comparison with previous studies, possibly because of increasing eutrophication and invasions of non-indigenous species (NIS). The aim of the present study was the identification of species which can be used as ecological indicators of high trophic status. Among the nine copepod species of Lake Manzalah, Acartia tonsa, Mesocyclops ogunnus, and Apocyclops panamensis were reported for the first time. Acartia tonsa, a well-known NIS for the Mediterranean, numerically dominated the copepod assemblages in some portions of the lake. The distribution of Acanthocyclops trajani and Thermocyclops consimilis was insensible to eutrophication because they can stand high levels of nutrients and hypoxia. Compared with previous reports, the copepod assemblage of Lake Manzalah was richer in species. The invasions of NIS, in addition to the heterogeneous progress of eutrophication in the lake, created an environmental mosaic with many species in total, but with single areas suitable for only a small number of them.


2020 ◽  
Vol 222 (6) ◽  
pp. 1046-1050 ◽  
Author(s):  
Matt D Johansen ◽  
Laurent Kremer

Abstract Mycobacterium kansasii is a slow-growing nontuberculous mycobacteria responsible for coinfections particularly in patients with human immunodeficiency virus. To date, our knowledge of M. kansasii infection has been hampered owing to the lack of an effective animal model to study pathogenesis. In the current study, we showed that the zebrafish embryo is permissive to M. kansasii infection, resulting in chronic infection and formation of granulomas. On macrophage depletion, we identified M. kansasii forms extracellular cords, resulting in acute infection and rapid larval death. These findings highlight the feasibility of zebrafish for studying M. kansasii pathogenesis and for the first time identify extracellular cords in this species.


Sign in / Sign up

Export Citation Format

Share Document