scholarly journals Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Joanna Berłowska ◽  
Katarzyna Pielech-Przybylska ◽  
Maria Balcerek ◽  
Urszula Dziekońska-Kubczak ◽  
Piotr Patelski ◽  
...  

Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter. Enzymatic hydrolysis was conducted using Viscozyme and Ultraflo Max (Novozymes) enzyme preparations (0.015–0.02 mL/g dry matter). Two yeast strains were used for fermentation: Ethanol Red (S. cerevisiae) (1 g/L) andPichia stipitis(0.5 g/L), applied sequentially. The results show that efficient simultaneous saccharification and fermentation of sugar beet pulp was achieved. A 6 h interval for enzymatic activation between the application of enzyme preparations and inoculation with Ethanol Red further improved the fermentation performance, with the highest ethanol concentration reaching26.9±1.2 g/L and86.5±2.1%fermentation efficiency relative to the theoretical yield.

Molecules ◽  
2016 ◽  
Vol 21 (10) ◽  
pp. 1380 ◽  
Author(s):  
Joanna Berlowska ◽  
Weronika Cieciura ◽  
Sebastian Borowski ◽  
Marta Dudkiewicz ◽  
Michal Binczarski ◽  
...  

1986 ◽  
Vol 43 (2) ◽  
pp. 351-354 ◽  
Author(s):  
J. J. Parkins ◽  
R. G. Hemingway ◽  
J. Fraser

ABSTRACTSugar-beet pulp as normally produced in Great Britain contains about 400 g molasses per kg dry matter (DM). It has been demonstrated that either a reduction in the amount or even the full removal of the molasses did not affect the yield or composition of the milk of cows (e.g. Ronning and Bath, 1962; Hemingway, Parkins and Fraser, 1986). The dried, shredded pulp without molasses was, however, generally less palatable.


2020 ◽  
Vol 13 (6) ◽  
pp. 923-936 ◽  
Author(s):  
Ewelina Strąk-Graczyk ◽  
Maria Balcerek

Abstract The rising population and increasing demand for food place added pressure on the agricultural sector to maintain high process efficiency while implementing environmentally friendly methods. In this study, we investigate the effect of pre-hydrolysis of native rye starch and its influence on the yield of ethanol obtained by simultaneous saccharification and fermentation (SSF) from high gravity rye mashes with 25% and 28% w w−1 dry matter content. Fermentation was carried out in a 3-day system at a temperature of 35 ± 1 °C using the dry distillery yeast Ethanol Red (Saccharomyces cerevisiae). The characteristics of the tested raw material and changes in the native rye starch during enzymatic hydrolysis were analyzed using a scanning electron microscope (SEM). The SEM images revealed characteristic changes on the surface of the starch, which was found to have a layered structure, as well as interesting behavior by the yeast during SSF when the glucose concentration in the environment was lowered. Both in the mashes with 25% and 28% w w−1 dry matter, starch pre-hydrolysis did not significantly increase either the initial amounts of sugars available to the yeast or the fermentation efficiency and ethanol yield in comparison to the mashes without this pre-treatment.


2011 ◽  
Vol 50 (No. 12) ◽  
pp. 553-560 ◽  
Author(s):  
P. Doležal ◽  
V. Pyrochta ◽  
J. Doležal

This study deals with effects of pressing of ensiled sugar-beet pulp and of application of a chemical preservative on the quality of fermentation process. The experimental silages had a better sensory evaluation than the control ones. In silages treated chemically with a mixture of acids, statistically significantly (P < 0.01) higher dry matter content, lowest pH value, the value of lactic acid and the lowest content of all acids in dry matter were found after 180 days of storage from the beginning of the experiment. The statistically significantly (P < 0.01) highest lactic acid content (43.39 ± 1.25 g/kg DM) was determined in the control pressed silage. The highest LA/VFA ratio (1.40 ± 0.18) was calculated for non-pressed experimental silage (D – 3 l/t of KEM). As compared with untreated control the highest percentage (P < 0.01) of lactic acid and of all fermentation acids was found out in silage D treated with 3 l/t of KEM (58.18 ± 0.47 g/kg DM). Undesirable butyric and propionic acids were not found in chemically treated silage samples (C, D, E, F). However, the highest (P < 0.01) contents of butyric acid (26.37 ± 0.91 g/DM) and propionic acid (4.58 ± 0.78 g/DM) were measured in untreated non-pressed silage samples (B). The highest (P < 0.01) contents of acetic acid and ethanol were found in control silage samples. The quality of these silages was evaluated as very low.  


1972 ◽  
Vol 78 (3) ◽  
pp. 371-377 ◽  
Author(s):  
M. E. Castle

SUMMARYTwo 16-week experiments with lactating Ayrshire cows were conducted to study the effect on milk production of replacing barley with dried molassed sugar-beet pulp on an equal dry-matter basis. The cows on all treatments received equal weights of hay, groundnut cake and minerals but the contents of barley and of sugar-beet pulp each varied from 0 to 80% in the different concentrate mixtures. The total daily intake of dry matter expressed as a percentage of live weight averaged 2·64 and 2·72 in the two experiments.The average yield of milk in the two experiments was 18·8 kg/cow per day, and, within each experiment, the mean yields of milk and the contents of solids-not-fat and crude protein were not significantly different on the contrasting feeding treatments. The intake of water was significantly increased as the dried beet pulp replaced the barley but no major changes occurred in the proportions of V.F.A.S in the rumen liquor.It is concluded that barley and dried molassed sugar-beet pulp had the same feeding value on a dry-matter basis when used in the production ration of milking cows. It is suggested that for most purposes barley and dried sugar-beet pulp are interchangeable on an equal weight basis, but for more precise feeding the two feeds should replace each other on an equal dry-weight basis.


1999 ◽  
Vol 1999 ◽  
pp. 140-140
Author(s):  
J. J. Hyslop ◽  
D. Cuddeford

There is increased interest in using sugar beet pulp (SBP) products as forage replacers in equine diets. Previous work (Hyslop et al 1998) has indicated that SBP products may suppress voluntary feed intake (VFI) in ponies when they are the major fibre source at between 550-700 g/kg total diet dry matter (DM). This study examines VFI and apparent digestibility in vivo where soaked unmolassed sugar beet pulp (USBP) was offered as a partial forage replacement to ponies receiving ad libitum grass hay.Four mature Welsh-cross pony geldings (280 kg LW s.e. 6.6) were individually housed and offered 0.86 kg/d of micronised barley (MB) and given ad libitum access to grass hay (HAY). In addition, ponies were offered either 0, 1, 2, or 3 kg/d USBP (U0, U1, U2 and U3 respectively) according to a 4 x 4 latin square changeover experimental design.


Author(s):  
P N Johnson

Silage effluent represents a potential pollutant and a loss of ensiled nutrients. An absorbent provides the possibility of absorbing effluent, aiding fermentation and increasing the feeding value of the silage. Sugar beet pulp (SBP) nuts and rolled barley (RB) were evaluated as absorbents with direct cut grass silage.Perennial ryegrass swards of 222 g/kg average dry matter were direct cut between 10 and 12 July 1989. Grass was ensiled with the following rates of absorbent per tonne of fresh material:1.20 kg SBP (S20).2.40 kg SBP (S40).3.40 kg RB (B40).4.Untreated (U).Production of silage effluent was measured with a tippler mechanism attached to an electronic logger and recorded daily until 22 September. The silages were individually fed through Calan Broadbent doors to 48 x 12 month old Friesian steers between 24 October and 19 December.


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