The Th17/Treg Immune Imbalance in Ulcerative Colitis Disease in a Chinese Han Population

Objective. To investigate the Th17/Treg immune balance in the ulcerative colitis (UC) patients in a Chinese Han population. Methods. Ninety UC patients and 30 healthy subjects were enrolled. The serum IL-17 and TGF-β1 levels of these participants were measured with ELISA; the percentage of Th17 and Treg cells in peripheral blood was determined with flow cytometry. Results. In UC patients, the levels of IL-17 and Th17 were significantly higher compared with healthy subjects; the percentage of Th17 and IL-17 level in moderate and severe subgroup was significantly higher than in mild subgroup; a positive correlation existed between these two indexes and clinical activity index and endoscopic evaluation. TGF-β1 level and Treg cells in UC patients were lower than healthy subjects. TGF-β1 level in moderate and severe subgroup was lower than in mild subgroup. There was a negative linear correlation between Treg cells and clinical activity index, endoscopic evaluation. A positive correlation was detected between Treg cells and TGF-β1 level. Conclusions. Th17/Treg immune imbalance might play a crucial role in the development of UC. To induce the production of Treg cells and TGF-β1, inhibit the level of Th17 and IL-17, and thus recover the Th17/Treg immune balance might imply new therapeutic targets in UC management.

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The protective effect of curcumin on rats with DSS-induced ulcerative colitis and its mechanisms

10.21203/rs.3.rs-191627/v1 ◽

2021 ◽

Author(s):

Jing Guo ◽

Yan-yan Zhang ◽

Mei Sun ◽

Ling-fen Xu

Keyword(s):

Ulcerative Colitis ◽

Th17 Cells ◽

Dextran Sulfate ◽

Activity Index ◽

Disease Activity Index ◽

Treg Cells ◽

Enzyme Linked Immunosorbent Assay ◽

T Helper ◽

T Helper 17 ◽

Inflammatory Bowel

Abstract Aim This study aimed to explore effect of curcumin on inflammatory bowel disease (IBD) in rats and its mechanism.Methods: A dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) rat model was established. The disease activity index (DAI) scores were calculated. The histopathological damage scores were determined by haematoxylin-eosin (H&E) staining. Regulatory T (Treg) cells and T helper 17 (Th17) cells in the spleen were analysed by flow cytometry. The levels of interleukin (IL)-10 and IL-17A were determined by enzyme-linked immunosorbent assay (ELISA). Results: Compared with the DSS model group, the curcumin group exhibited significantly reduced DAI scores and improvements in histopathological damage. The expression of CD4+IL-17+ Th17 cells was significantly lower and the expression of CD4+CD25+Foxp3+ Treg cells was significantly higher in the curcumin group than in the DSS group.Conclusion: Curcumin may be a new and effective treatment for IBD by regulating the balance of Treg/Th17 cells and the expression of IL-10 and IL-17A.

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P187 The significance of netrin-1 level in the clinical activity of ulcerative colitis, its association between TNF-α and IL-6

Journal of Crohn s and Colitis ◽

10.1093/ecco-jcc/jjz203.316 ◽

2020 ◽

Vol 14 (Supplement_1) ◽

pp. S232-S232

Author(s):

H Korkmaz ◽

K Fidan

Keyword(s):

Ulcerative Colitis ◽

Proinflammatory Cytokines ◽

Activity Index ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Clinical Activity ◽

Tnf Α ◽

Significant Difference ◽

And Control ◽

Control Study

Abstract Background In this study, we investigated the importance of netrin-1 levels in ulcerative colitis (UC) in clinical activity of the disease, and its association with other proinflammatory cytokines IL-6 and TNF-α. Methods This study is a type of case–control study. Sixty-seven patients with UC (36 of them activation, 31 of remission) and 50 healthy controls were included in the study. UC patients; ‘Truelove Witts clinical activity index by remission (n = 31), mild activation (n = 21), moderate activation (n = 6) and severe activation (n = 9) were divided into groups. Netrin, IL-6 and TNF-α measurements in plasma samples were performed using enzyme-linked immunosorbent assay kit. Results Between the patient group and the control group; there was a statistically significant difference between netrin-1, IL-6, TNF-α, neutrophil, platelet (p < 0.05 for all). The plasma netrin-1 mean of UC with severe activation group (139.21 ± 48.09 pg/ml) was statistically significantly higher than that of the mild activation (p = 0,037), remission group (p = 0,001) and control group(p = 0,011). The plasma netrin-1 mean of UC with moderate activation group was statistically significantly higher than that of the mild activation(p = 0,045) and remission group(p = 0,004). Conclusion Our results reveal that plasma netrin-1 levels have been shown to be associated with UC activation, similar to proinflammatory cytokines such as TNF-α and IL-6, in UC.

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P471 What is the appropriate cut-off value of CRP to predict endoscopic remission in ulcerative colitis patients with clinical remission?

Journal of Crohn s and Colitis ◽

10.1093/ecco-jcc/jjz203.600 ◽

2020 ◽

Vol 14 (Supplement_1) ◽

pp. S415-S415

Author(s):

J Shin ◽

G Seong ◽

J H Song ◽

S M Kong ◽

T J Kim ◽

...

Keyword(s):

Ulcerative Colitis ◽

Sensitivity And Specificity ◽

Clinical Remission ◽

Activity Index ◽

Area Under The Curve ◽

Endoscopic Evaluation ◽

Faecal Calprotectin ◽

Reactive Protein ◽

Endoscopic Remission ◽

Low Sensitivity

Abstract Background A noninvasive and reliable markers for predicting endoscopic remission (ER) in ulcerative colitis (UC) patients with clinical remission (CR) provide important information in predicting disease progression and in determining treatment. Faecal calprotectin test is known to be the most accurate to predict ER, but patients are reluctant to handle faecal materials. C-reactive protein (CRP) is one of the surrogate markers for assessing disease activity, but it is known to have low sensitivity and specificity of normal CRP value (<0.3 mg/dl). The sensitivity of the CRP test has been improved, and even fine values within the normal range can be measured. The aim of this study was to determine appropriate CRP cut-off values for the prediction of ER in UC patients with CR even though within normal CRP range. Methods A total of 132 UC patients who underwent endoscopic evaluation in CR were retrospectively reviewed. Serum biomarkers including haemoglobin, leukocytes, platelets, erythrocyte sedimentation rate, and CRP were evaluated within 1 week period from endoscopic evaluation. The clinical and endoscopic activity was measured by simple clinical colitis activity index and endoscopic Mayo subscore. Results In UC patient with CR, CRP level was significantly lower in ER (median 0.05, 0.03–2.57) vs. non-ER (median 0.11 0.03-2.81). (p < 0.005) The proportion of males in non-ER was slightly higher than in ER (24, 72.7% vs. 52, 52.5 %; p = 0.042), and only gender and CRP showed statistical differences in baseline clinical characteristics. CRP had predictive value of ER [Area under the curve (AUC = 0.760)] and the sensitivity was 71.4%, specificity was 71.7 % at cut-off value of 0.09mg/dl. In contrast, the sensitivity and specificity of normal CRP (0.3mg/dl) were low. (sensitivity 27.3%, specificity 90.9%). Conclusion Norma CRP cut-off values are not sufficient to reflect ER. It may be helpful to change the CRP cut-off value that predicts ER in CR to value other than 0.3 mg/dl.

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Su1259 Additional Item of Fecal Hemoglobin As a Marker for Mucosal Inflammation to Rachmilewitz's Clinical Activity Index Is Useful for the Assessment of the Activity of Ulcerative Colitis

Gastroenterology ◽

10.1016/s0016-5085(15)31530-4 ◽

2015 ◽

Vol 148 (4) ◽

pp. S-453-S-454

Author(s):

Shigeru Kanaoka ◽

Shigeru Kuriyama ◽

Shigeto Yoshii ◽

Yasushi Iwaoka ◽

Moriya Iwaizumi ◽

...

Keyword(s):

Ulcerative Colitis ◽

Activity Index ◽

Mucosal Inflammation ◽

Clinical Activity ◽

Additional Item

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Association of Single Nucleotide Polymorphisms of IL23R and IL17 with Ulcerative Colitis Risk in a Chinese Han Population

PLoS ONE ◽

10.1371/journal.pone.0044380 ◽

2012 ◽

Vol 7 (9) ◽

pp. e44380 ◽

Cited By ~ 15

Author(s):

Pengli Yu ◽

Fangcheng Shen ◽

Xiaofei Zhang ◽

Risheng Cao ◽

Xiaodan Zhao ◽

...

Keyword(s):

Ulcerative Colitis ◽

Single Nucleotide Polymorphisms ◽

Chinese Han Population ◽

Nucleotide Polymorphisms ◽

Chinese Han ◽

Single Nucleotide ◽

Han Population

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The shifted balance between circulating follicular regulatory T cells and follicular helper T cells in patients with ulcerative colitis

Clinical Science ◽

10.1042/cs20171258 ◽

2017 ◽

Vol 131 (24) ◽

pp. 2933-2945 ◽

Cited By ~ 12

Author(s):

Xinrui Wang ◽

Yonggang Zhu ◽

Manli Zhang ◽

Jie Hou ◽

Hongjuan Wang ◽

...

Keyword(s):

Ulcerative Colitis ◽

T Cells ◽

B Cell ◽

Mayo Clinic ◽

Treg Cells ◽

Tfh Cells ◽

Follicular Helper ◽

Immune Balance ◽

Cell Immunity ◽

B Cell Immunity

B-cell immunity participates in the pathogenesis of ulcerative colitis (UC). The immune balance between follicular regulatory T (TFR) cells and follicular helper T (TFH) cells is important in regulating B-cell responses. However, the alteration of TFR/TFH balance in UC remains unclear. Peripheral blood from 25 UC patients and 15 healthy controls was examined for the frequencies of circulating TFR, TFH, and regulatory T (Treg) cells by flow cytometry. Levels of serum cytokines were measured using cytometric bead array (CBA). Disease activity was evaluated by the Mayo Clinic Score. Compared with controls, UC patients exhibited significant reductions in circulating Foxp3+CXCR5+ TFR cells, the subset interleukin (IL)-10+Foxp3+CXCR5+ cells, and Treg cells, but significant expansions in Foxp3−CXCR5+ TFH cells and IL-21+Foxp3−CXCR5+ cells. UC patients also had reduced levels of serum IL-10 and elevated levels of serum IL-21. The values of Mayo Clinic Score, C-reactive protein (CRP), or erythrocyte sedimentation rate (ESR) in UC patients were negatively correlated with circulating TFR cells, serum IL-10 level, and TFR/TFH ratio, while positively correlated with circulating TFH cells and serum IL-21 level. Alterations in circulating TFR and TFH cells shift the balance from immune tolerance to immune responsive state, contributing to dysregulated B-cell immunity and the pathogenesis of UC.

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