Bactericide Effect of Silver Nanoparticles as a Final Irrigation Agent in Endodontics onEnterococcus faecalis: AnEx VivoStudy

The objective of this study was to determine the bactericidal effect of silver nanoparticles as a final irrigation agent in endodontics. This study included 120 uniradicular extracted dental organs inoculated withEnterococcus faecalis(E. faecalis) and organized into 4 groups: (A) 30 teeth irrigated with a dispersion of silver nanoparticles (537 μg/mL); (B) 30 teeth irrigated with a sodium hypochlorite solution (2.25%); (C) 30 teeth irrigated with a dispersion of silver nanoparticles (537 μg/mL) + EDTA (17%); and (D) 30 teeth with a saline solution. After the irrigation protocol, the samples were analyzed through a spectrophotometer to measure the bactericidal effect and scanning electron microscope and atomic force microscope in order to observe the presence of dental smear layer. The results showed that nanoparticles of 10 nm and the sodium hypochlorite at 2.25% were effective for eliminatingE. faecalis, with no significant difference between them.

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IMBIBITION EFFECT OF IMMERSION HYDROCOLLOID IRREVERSIBLE ALGINATE IN SODIUM HYPOCHLORITE SOLUTIO

Andalas Dental Journal ◽

10.25077/adj.v3i2.51 ◽

2015 ◽

Vol 3 (2) ◽

pp. 59-67

Author(s):

Siti Coryniken ◽

Didin Kustantiningtyastuti ◽

Afriwardi Afriwardi

Keyword(s):

Sodium Hypochlorite ◽

Repeated Measure ◽

Control Group ◽

Sodium Hypochlorite Solution ◽

Control Group Design ◽

Impression Material ◽

Hypochlorite Solution ◽

Significant Difference ◽

Weight Calculation ◽

Disinfectant Solution

materials have character that absorb water it is imbibition that can affect the dimensional stability when immersed in disinfectant . The purpose of this study is to see the effect of imbibition at alginate impression material immersed in a disinfectant solution of Sodium Hypochlorite .The method used was experimental with pretest and posttest with control group design . The mold were diameter of 28 mm and height 18 mm .the impression were immersed in sodium hypochlorite 0.5 % and 1 % for 3 minutes , 5 minutes and 10 minutes . Imbibition weight calculation is done by using digital scales.The results showed that the presence of imbibition on impression material alginate that immersed in Sodium Hypochlorite solution for 3 minutes , 5 minutes and 10 minutes . Statistical Test Two Way Repeated Measure ANOVA showed a significant difference ( p< 0.05 ) in minutes imbibition , while the concentration of the solution had a significant difference ( p > 0.05 ) .The conclusion of this study is the alginate impression material has the effect of imbibition to immersion Sodium Hypochlorite solution. Keywords : hydrocolloid Alginate , Sodium Hypochlorite , imbibition effects , immersio

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Inactivation of heat adapted and chlorine adapted Listeria monocytogenes ATCC 7644 on tomatoes using sodium dodecyl sulphate, levulinic acid and sodium hypochlorite solution

Italian Journal of Food Safety ◽

10.4081/ijfs.2017.5916 ◽

2017 ◽

Vol 6 (2) ◽

Cited By ~ 1

Author(s):

Oluwatosin Ademola Ijabadeniyi ◽

Elizabeth Mnyandu

Keyword(s):

Listeria Monocytogenes ◽

Sodium Hypochlorite ◽

Sodium Dodecyl Sulphate ◽

Levulinic Acid ◽

Sodium Dodecyl ◽

Storage Period ◽

Sodium Hypochlorite Solution ◽

Hypochlorite Solution ◽

Log Reduction ◽

Significant Difference

The effectiveness of sodium dodecyl sulphate (SDS), sodium hypochlorite solution and levulinic acid in reducing the survival of heat adapted and chlorine adapted Listeria monocytogenes ATCC 7644 was evaluated. The results against heat adapted L. monocytognes revealed that sodium hypochlorite solution was the least effective, achieving log reduction of 2.75, 2.94 and 3.97 log CFU/mL for 1, 3 and 5 minutes, respectively. SDS was able to achieve 8 log reduction for both heat adapted and chlorine adapted bacteria. When used against chlorine adapted L. monocytogenes sodium hypochlorite solution achieved log reduction of 2.76, 2.93 and 3.65 log CFU/mL for 1, 3 and 5 minutes, respectively. Using levulinic acid on heat adapted bacteria achieved log reduction of 3.07, 2.78 and 4.97 log CFU/mL for 1, 3, 5 minutes, respectively. On chlorine adapted bacteria levulinic acid achieved log reduction of 2.77, 3.07 and 5.21 log CFU/mL for 1, 3 and 5 minutes, respectively. Using a mixture of 0.05% SDS and 0.5% levulinic acid on heat adapted bacteria achieved log reduction of 3.13, 3.32 and 4.79 log CFU/mL for 1, 3 and 5 minutes while on chlorine adapted bacteria it achieved 3.20, 3.33 and 5.66 log CFU/mL, respectively. Increasing contact time also increased log reduction for both test pathogens. A storage period of up to 72 hours resulted in progressive log reduction for both test pathogens. Results also revealed that there was a significant difference (P ≤ 0.05) between contact times, storage times and sanitizers. Findings from this study can be used to select suitable sanitizers and contact times for heat adapted and chlorine adapted L. monocytogenes in the fresh produce industry.

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Structural effects of sodium hypochlorite solution on RaCe rotary nickel-titanium instruments: an atomic force microscopy study

Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology ◽

10.1016/j.tripleo.2007.11.006 ◽

2008 ◽

Vol 105 (5) ◽

pp. 661-665 ◽

Cited By ~ 18

Author(s):

Ozgur Topuz ◽

Cumhur Aydin ◽

Ozgur Uzun ◽

Ugur Inan ◽

Tayfun Alacam ◽

...

Keyword(s):

Atomic Force Microscopy ◽

Sodium Hypochlorite ◽

Microscopy Study ◽

Nickel Titanium ◽

Sodium Hypochlorite Solution ◽

Structural Effects ◽

Atomic Force Microscopy Study ◽

Hypochlorite Solution ◽

Force Microscopy ◽

Atomic Force

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PERBEDAAN STABILITAS DIMENSI ANTARA CETAKAN ALGINAT YANG DISEMPROT DENGAN LARUTAN NATRIUM HIPOKLORIT 0,5% DAN DETTOL 5%

Andalas Dental Journal ◽

10.25077/adj.v5i1.71 ◽

2017 ◽

Vol 5 (1) ◽

pp. 59-70

Author(s):

Wira Putri Winata ◽

Kuswardani Susari Putri ◽

Febrian Febrian

Keyword(s):

Sodium Hypochlorite ◽

Dimensional Stability ◽

Control Group ◽

Sodium Hypochlorite Solution ◽

Control Group Design ◽

Hypochlorite Solution ◽

Impression Materials ◽

Significant Difference ◽

Post Test ◽

The Difference

The impression materials that most commonly used in dentistry was alginate. Factors that must be considered was the controlled of infection transmission from alginate, it was important to do disinfection using a disinfectant solution. Alginate had imbibition properties, so that disinfection by spraying technique allowed the expansion of alginate affecting the dimensional stability of impressions. The purpose of this research was to determine the difference between the dimensional stability of alginate impression were sprayed with a solution of sodium hypochlorite 0,5% and Dettol® 5%. The method used was an experimental laboratory with post test only control group design. 27 samples were divided into 3 groups: spraying with 0.5% sodium hypochlorite solution, spraying with 5% Dettol® solution, and spraying with aquades as a controlled. Alginate impressions were sprayed with 0,5% sodium hypochlorite solution, Dettol® 5%, and aquades then filled with gypsum, and then measured dimensional stability by using micrometer screw. Data were analyzed with the Kruskal-Wallis test. The results showed that differences between the dimensional stability of alginate impressions were sprayed with a solution of sodium hypochlorite 0.5% and 5% Dettol® was not significant (p> 0,05). The conclusion of this research was there was no significant difference between the alginate impressions were sprayed with a solution of sodium hypochlorite 0,5% and Dettol® 5% to dimensional stability. Keywords : dimensional stability, alginate, sprayed, sodium hypochlorite, Dettol®

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Comparison of the Clinical Efficacy of 2.5% Non-Heated Sodium Hypochlorite and 2.5% Heated Sodium Hypochlorite as Irrigation to Control Postoperative Pain in Single Visit Root Canal Treatment

10.53350/pjmhs211582220 ◽

2021 ◽

Vol 15 (8) ◽

pp. 2220-2223

Author(s):

Muhammad Waleed ◽

Farah Tasleem ◽

Sarwanand . ◽

Noorullah Jagirani ◽

Fozia Rajput ◽

...

Keyword(s):

Postoperative Pain ◽

Clinical Efficacy ◽

Sodium Hypochlorite ◽

Root Canal ◽

Root Canal Treatment ◽

Sodium Hypochlorite Solution ◽

Hypochlorite Solution ◽

Single Visit ◽

Significant Difference ◽

Reduction Of Pain

Objective: To compare the clinical efficacy of 2.5% non-heated sodium hypochlorite and 2.5% heated sodium hypochlorite as irrigation to control postoperative pain in single visit root canal treatment. Study Design: Comparative analytical study Place and Duration: Operative dentistry department of Liaquat University of Medical and Health Sciences, Jamshoro for 6 months duration from March 2017 to September 2017. Material and Methods: A total of 60 patients with single rooted maxillary and mandibular permanent teeth were randomly allocated into two groups. 30 patients were in group A and treated with non heated sodium hypochlorite solution and 30 patients in group B were treated with 2.5% heated sodium hypochlorite solution. In both groups, the level of pain was measured postoperatively after 48 hours by using Heft Parker visual Analog Scale of 10 and information was collected on Performa. Results: The average age of the patients was 28.62±6.06 years. There were 40(66.7%) male and 20(33.3%) female. Pain was reduced in both groups but there were no significant difference in reduction of pain between groups (p=0.640). Conclusion: Severity of pain was not statistically significant between groups. At 48 hours, Pain was reduced in both groups but there were no significant difference in reduction of pain between groups. Heated sodium hypochlorite to relieve postoperative pain, it should be new trend for recommendation for treatment of irreversible pulpitis in single visit root canal treatment. Key Words: Root canal, Heated sodium hypochlorite, Postoperative pain.

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Effect of alteration in organic material of the occlusal caries on DIAGNOdent readings

Brazilian Oral Research ◽

10.1590/s1806-83242004000200009 ◽

2004 ◽

Vol 18 (2) ◽

pp. 141-144 ◽

Cited By ~ 15

Author(s):

Fausto Medeiros Mendes ◽

Sérgio Luiz Pinheiro ◽

Antonio Lucindo Bengtson

Keyword(s):

Sodium Hypochlorite ◽

Organic Material ◽

Saline Solution ◽

Organic Content ◽

Laser Fluorescence ◽

Control Group ◽

Sodium Hypochlorite Solution ◽

Occlusal Caries ◽

Hypochlorite Solution ◽

Carious Lesions

DIAGNOdent is a laser fluorescence device used for dental caries diagnosis in occlusal and smooth surfaces. Despite the promising preliminary results, the molecules involved in the increase of fluorescence in carious lesions remain unclear. The aim of this study was to compare the laser fluorescence readings before and after changes in the organic material of occlusal carious lesions in primary teeth. Twenty-four primary molars stored in saline solution with at least one site with occlusal caries were divided into two groups. The control group had 17 sites with caries and the experimental one had 16 sites. The carious lesions were measured with laser fluorescence. The experimental samples were then removed from the storage solution and immersed in a 2% sodium hypochlorite solution for 24 hours. After washing with water, the teeth were measured again with the laser fluorescence device. The teeth of the control group were submitted to the same procedures, but saline solution was used instead of the sodium hypochlorite solution. A statistically significant reduction in the mean of the readings after immersion in the two tested solutions compared with the initial readings was observed in both groups, but the decrease was statistically higher in the experimental group (p < 0.0001). In this study, the data indicate that changes in the fluorescence of carious lesions measured by the laser fluorescence are mainly due to the organic content alterations rather than to the mineral loss.

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In vitro study of the effect of Er: YAG laser irradiation on the apical sealing of different root canal sealers

Journal of Applied Oral Science ◽

10.1590/s1678-77572006000400009 ◽

2006 ◽

Vol 14 (4) ◽

pp. 260-263 ◽

Cited By ~ 1

Author(s):

Fernanda Vieira Medina ◽

Manoel Damião Souza-Neto ◽

Jacy Ribeiro Carvalho-Junior ◽

Heid Sueli Leme dos Santos ◽

Melissa Albanese Mezzena ◽

...

Keyword(s):

Laser Irradiation ◽

Sodium Hypochlorite ◽

Root Canal ◽

Er:Yag Laser ◽

Sodium Hypochlorite Solution ◽

Laser Application ◽

Hypochlorite Solution ◽

Root Canals ◽

Group I ◽

Significant Difference

OBJECTIVES: The aim of this study was to determine the effect of Er:YAG laser irradiation used to clean dentinal walls on the apical sealing of root canals filled with different types of sealers. Background Data: Laser application to the dentinal walls removed debris, rendering the root canals free of smear layers and leaving the dentinal canaliculi open. METHODS: Sixty-four maxillary canines obtained from laboratory files were instrumented with K-files (Dentsply, Maillefer, Ballaigues, Switzerland) using the crown-down technique, and irrigated with a 0.5% sodium hypochlorite solution. The specimens were divided into two groups of 32 teeth each. In group I, the teeth were instrumented and irrigated with sodium hypochlorite solution, and divided into four subgroups to be sealed with the different materials (Endofill, N-Rickert, Sealapex and Sealer 26). In group II, the root canals were subjected to Er:YAG laser irradiation (200 mJ, 7 Hz and 60 J total energy), followed by root canal sealing as in group I. RESULTS: The data showed lower levels of apical microleakage in the teeth filled with N-Rickert, Sealapex and Sealer 26 cements than in those sealed with Endofill (p < 0.01). No significant difference in microleakage was observed between teeth irrigated only with 0.5% sodium hypochlorite and those submitted to Er:YAG laser application (p > 0.05). CONCLUSIONS: The Er:YAG laser irradiation applied to the root canal walls was not able to prevent apical microleakage.

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Efficacy of Electrolyzed Water in the Inactivation of Planktonic and Biofilm Listeria monocytogenes in the Presence of Organic Matter

Journal of Food Protection ◽

10.4315/0362-028x-69.9.2143 ◽

2006 ◽

Vol 69 (9) ◽

pp. 2143-2150 ◽

Cited By ~ 49

Author(s):

BEATRICE AYEBAH ◽

YEN-CON HUNG ◽

CHYER KIM ◽

JOSEPH F. FRANK

Keyword(s):

Organic Matter ◽

Listeria Monocytogenes ◽

Serum Concentration ◽

Sodium Hypochlorite ◽

Bactericidal Effect ◽

Sodium Hypochlorite Solution ◽

Chicken Serum ◽

Hypochlorite Solution ◽

Log Reduction ◽

Oxidation Reduction

The ability of electrolyzed (EO) water to inactivate Listeria monocytogenes in suspension and biofilms on stainless steel in the presence of organic matter (sterile filtered chicken serum) was investigated. A five-strain mixture of L. monocytogenes was treated with deionized, alkaline EO, and acidic EO water containing chicken serum (0, 5, and 10 ml/liter) for 1 and 5 min. Coupons containing L. monocytogenes biofilms were also overlaid with chicken serum (0, 2.5, 5.0, and 7.5 ml/liter) and then treated with deionized water, alkaline EO water, acidic EO water, alkaline EO water followed by acidic EO water, and a sodium hypochlorite solution for 30 and 60 s. Chicken serum decreased the oxidation-reduction potential and chlorine concentration of acidic EO water but did not significantly affect its pH. In the absence of serum, acidic EO water containing chlorine at a concentration of 44 mg/liter produced a >6-log reduction in L. monocytogenes in suspension, but its bactericidal activity decreased with increasing serum concentration. Acidic EO water and acidified sodium hypochlorite solution inactivated L. monocytogenes biofilms to similar levels, and their bactericidal effect decreased with increasing serum concentration and increased with increasing time of exposure. The sequential 30-s treatment of alkaline EO water followed by acidic EO water produced 4- to 5-log reductions in L. monocytogenes biofilms, even in the presence of organic matter.

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Evaluation of the surface of root canal walls after utilization of endodontic rotary systems: SEM study

Journal of Applied Oral Science ◽

10.1590/s1678-77572005000100016 ◽

2005 ◽

Vol 13 (1) ◽

pp. 78-82

Author(s):

José Carlos Yamashita ◽

Marco Antonio Hungaro Duarte ◽

Fernanda Araújo Valim ◽

Juliano Milanezi de Almeida ◽

Milton Carlos Kuga ◽

...

Keyword(s):

Root Canal ◽

Smear Layer ◽

Sodium Hypochlorite Solution ◽

Root Canal Preparation ◽

Hypochlorite Solution ◽

Root Canals ◽

System P ◽

Significant Difference ◽

Sem Study ◽

Scanning Electron

The objective of the present study was to determine, by scanning electron microscopy, the aspect of root canal walls after utilization of different rotary instrumentation systems for root canal preparation. Forty-two single-rooted and straight teeth were divided into six groups, whose root canals were prepared using the following rotary systems: groups 1 and 2 - Quantec; groups 3 and 4 - Pow-R; groups 5 and 6 - Profile. Irrigation was performed with 2.5% sodium hypochlorite solution in all groups, while only groups 2, 4 and 6 received a final flush with EDTA. Data were analyzed by non-parametric Kruskal-Wallis and Miller tests. Removal of the smear layer and other debris was more effective in the groups receiving EDTA as final irrigant. The Profile system showed the best cleaning, generating only a minimum smear layer, with a significant difference being observed compared to the Pow-R system (p<0.05), but no difference when compared with Quantec system. The final use of EDTA favored cleaner surfaces in all groups.

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